Arbuscular mycorrhizae improve low temperature tolerance in cucumber via alterations in H2O2 accumulation and ATPase activity.
Identifieur interne : 001752 ( Main/Corpus ); précédent : 001751; suivant : 001753Arbuscular mycorrhizae improve low temperature tolerance in cucumber via alterations in H2O2 accumulation and ATPase activity.
Auteurs : Airong Liu ; Shuangchen Chen ; Rui Chang ; Dilin Liu ; Haoran Chen ; Golam Jalal Ahammed ; Xiaomin Lin ; Chaoxing HeSource :
- Journal of plant research [ 1618-0860 ] ; 2014.
English descriptors
- KwdEn :
- Adenosine Triphosphatases (genetics), Adenosine Triphosphatases (metabolism), Cold Temperature (MeSH), Cucumis sativus (genetics), Cucumis sativus (microbiology), Cucumis sativus (physiology), Glomeromycota (physiology), Hydrogen Peroxide (metabolism), Mycorrhizae (physiology), Plant Proteins (genetics), Plant Proteins (metabolism).
- MESH :
- chemical , genetics : Adenosine Triphosphatases, Plant Proteins.
- chemical , metabolism : Adenosine Triphosphatases, Hydrogen Peroxide, Plant Proteins.
- genetics : Cucumis sativus.
- microbiology : Cucumis sativus.
- physiology : Cucumis sativus, Glomeromycota, Mycorrhizae.
- Cold Temperature.
Abstract
The combined effects of arbuscular mycorrhizal fungi (AMF) and low temperature (LT) on cucumber plants were investigated with respect to biomass production, H2O2 accumulation, NADPH oxidase, ATPase activity and related gene expression. Mycorrhizal colonization ratio was gradually increased after AMF-inoculation. However, LT significantly decreased mycorrhizal colonization ability and mycorrhizal dependency. Regardless of temperature, the total fresh and dry mass, and root activity of AMF-inoculated plants were significantly higher than that of the non-AMF control. The H2O2 accumulation in AMF-inoculated roots was decreased by 42.44% compared with the control under LT. H2O2 predominantly accumulated on the cell walls of apoplast but was hardly detectable in the cytosol or organelles of roots. Again, NADPH oxidase activity involved in H2O2 production was significantly reduced by AMF inoculation under LT. AMF-inoculation remarkably increased the activities of P-type H(+)-ATPase, P-Ca(2+)-ATPase, V-type H(+)-ATPase, total ATPase activity, ATP concentration and plasma membrane protein content in the roots under LT. Additionally, ATP concentration and expression of plasma membrane ATPase genes were increased by AMF-inoculation. These results indicate that NADPH oxidase and ATPase might play an important role in AMF-mediated tolerance to chilling stress, thereby maintaining a lower H2O2 accumulation in the roots of cucumber.
DOI: 10.1007/s10265-014-0657-8
PubMed: 25160659
Links to Exploration step
pubmed:25160659Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Arbuscular mycorrhizae improve low temperature tolerance in cucumber via alterations in H2O2 accumulation and ATPase activity.</title><author><name sortKey="Liu, Airong" sort="Liu, Airong" uniqKey="Liu A" first="Airong" last="Liu">Airong Liu</name><affiliation><nlm:affiliation>College of Forestry, Henan University of Science and Technology, Luoyang, 471003, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Shuangchen" sort="Chen, Shuangchen" uniqKey="Chen S" first="Shuangchen" last="Chen">Shuangchen Chen</name></author><author><name sortKey="Chang, Rui" sort="Chang, Rui" uniqKey="Chang R" first="Rui" last="Chang">Rui Chang</name></author><author><name sortKey="Liu, Dilin" sort="Liu, Dilin" uniqKey="Liu D" first="Dilin" last="Liu">Dilin Liu</name></author><author><name sortKey="Chen, Haoran" sort="Chen, Haoran" uniqKey="Chen H" first="Haoran" last="Chen">Haoran Chen</name></author><author><name sortKey="Ahammed, Golam Jalal" sort="Ahammed, Golam Jalal" uniqKey="Ahammed G" first="Golam Jalal" last="Ahammed">Golam Jalal Ahammed</name></author><author><name sortKey="Lin, Xiaomin" sort="Lin, Xiaomin" uniqKey="Lin X" first="Xiaomin" last="Lin">Xiaomin Lin</name></author><author><name sortKey="He, Chaoxing" sort="He, Chaoxing" uniqKey="He C" first="Chaoxing" last="He">Chaoxing He</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:25160659</idno><idno type="pmid">25160659</idno><idno type="doi">10.1007/s10265-014-0657-8</idno><idno type="wicri:Area/Main/Corpus">001752</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001752</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Arbuscular mycorrhizae improve low temperature tolerance in cucumber via alterations in H2O2 accumulation and ATPase activity.</title><author><name sortKey="Liu, Airong" sort="Liu, Airong" uniqKey="Liu A" first="Airong" last="Liu">Airong Liu</name><affiliation><nlm:affiliation>College of Forestry, Henan University of Science and Technology, Luoyang, 471003, People's Republic of China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Shuangchen" sort="Chen, Shuangchen" uniqKey="Chen S" first="Shuangchen" last="Chen">Shuangchen Chen</name></author><author><name sortKey="Chang, Rui" sort="Chang, Rui" uniqKey="Chang R" first="Rui" last="Chang">Rui Chang</name></author><author><name sortKey="Liu, Dilin" sort="Liu, Dilin" uniqKey="Liu D" first="Dilin" last="Liu">Dilin Liu</name></author><author><name sortKey="Chen, Haoran" sort="Chen, Haoran" uniqKey="Chen H" first="Haoran" last="Chen">Haoran Chen</name></author><author><name sortKey="Ahammed, Golam Jalal" sort="Ahammed, Golam Jalal" uniqKey="Ahammed G" first="Golam Jalal" last="Ahammed">Golam Jalal Ahammed</name></author><author><name sortKey="Lin, Xiaomin" sort="Lin, Xiaomin" uniqKey="Lin X" first="Xiaomin" last="Lin">Xiaomin Lin</name></author><author><name sortKey="He, Chaoxing" sort="He, Chaoxing" uniqKey="He C" first="Chaoxing" last="He">Chaoxing He</name></author></analytic><series><title level="j">Journal of plant research</title><idno type="eISSN">1618-0860</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphatases (genetics)</term><term>Adenosine Triphosphatases (metabolism)</term><term>Cold Temperature (MeSH)</term><term>Cucumis sativus (genetics)</term><term>Cucumis sativus (microbiology)</term><term>Cucumis sativus (physiology)</term><term>Glomeromycota (physiology)</term><term>Hydrogen Peroxide (metabolism)</term><term>Mycorrhizae (physiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Adenosine Triphosphatases</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphatases</term><term>Hydrogen Peroxide</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cucumis sativus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Cucumis sativus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cucumis sativus</term><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cold Temperature</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The combined effects of arbuscular mycorrhizal fungi (AMF) and low temperature (LT) on cucumber plants were investigated with respect to biomass production, H2O2 accumulation, NADPH oxidase, ATPase activity and related gene expression. Mycorrhizal colonization ratio was gradually increased after AMF-inoculation. However, LT significantly decreased mycorrhizal colonization ability and mycorrhizal dependency. Regardless of temperature, the total fresh and dry mass, and root activity of AMF-inoculated plants were significantly higher than that of the non-AMF control. The H2O2 accumulation in AMF-inoculated roots was decreased by 42.44% compared with the control under LT. H2O2 predominantly accumulated on the cell walls of apoplast but was hardly detectable in the cytosol or organelles of roots. Again, NADPH oxidase activity involved in H2O2 production was significantly reduced by AMF inoculation under LT. AMF-inoculation remarkably increased the activities of P-type H(+)-ATPase, P-Ca(2+)-ATPase, V-type H(+)-ATPase, total ATPase activity, ATP concentration and plasma membrane protein content in the roots under LT. Additionally, ATP concentration and expression of plasma membrane ATPase genes were increased by AMF-inoculation. These results indicate that NADPH oxidase and ATPase might play an important role in AMF-mediated tolerance to chilling stress, thereby maintaining a lower H2O2 accumulation in the roots of cucumber.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25160659</PMID><DateCompleted><Year>2015</Year><Month>10</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0860</ISSN><JournalIssue CitedMedium="Internet"><Volume>127</Volume><Issue>6</Issue><PubDate><Year>2014</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of plant research</Title><ISOAbbreviation>J Plant Res</ISOAbbreviation></Journal><ArticleTitle>Arbuscular mycorrhizae improve low temperature tolerance in cucumber via alterations in H2O2 accumulation and ATPase activity.</ArticleTitle><Pagination><MedlinePgn>775-85</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10265-014-0657-8</ELocationID><Abstract><AbstractText>The combined effects of arbuscular mycorrhizal fungi (AMF) and low temperature (LT) on cucumber plants were investigated with respect to biomass production, H2O2 accumulation, NADPH oxidase, ATPase activity and related gene expression. Mycorrhizal colonization ratio was gradually increased after AMF-inoculation. However, LT significantly decreased mycorrhizal colonization ability and mycorrhizal dependency. Regardless of temperature, the total fresh and dry mass, and root activity of AMF-inoculated plants were significantly higher than that of the non-AMF control. The H2O2 accumulation in AMF-inoculated roots was decreased by 42.44% compared with the control under LT. H2O2 predominantly accumulated on the cell walls of apoplast but was hardly detectable in the cytosol or organelles of roots. Again, NADPH oxidase activity involved in H2O2 production was significantly reduced by AMF inoculation under LT. AMF-inoculation remarkably increased the activities of P-type H(+)-ATPase, P-Ca(2+)-ATPase, V-type H(+)-ATPase, total ATPase activity, ATP concentration and plasma membrane protein content in the roots under LT. Additionally, ATP concentration and expression of plasma membrane ATPase genes were increased by AMF-inoculation. These results indicate that NADPH oxidase and ATPase might play an important role in AMF-mediated tolerance to chilling stress, thereby maintaining a lower H2O2 accumulation in the roots of cucumber.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Airong</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>College of Forestry, Henan University of Science and Technology, Luoyang, 471003, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shuangchen</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Chang</LastName><ForeName>Rui</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Dilin</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Haoran</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Ahammed</LastName><ForeName>Golam Jalal</ForeName><Initials>GJ</Initials></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Xiaomin</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>He</LastName><ForeName>Chaoxing</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>08</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Plant Res</MedlineTA><NlmUniqueID>9887853</NlmUniqueID><ISSNLinking>0918-9440</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.6.1.-</RegistryNumber><NameOfSubstance UI="D000251">Adenosine Triphosphatases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000251" MajorTopicYN="N">Adenosine Triphosphatases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003080" MajorTopicYN="N">Cold Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018553" MajorTopicYN="N">Cucumis sativus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>11</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>06</Month><Day>25</Day></PubMedPubDate><PubMedPubDate 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