Proteomic analysis reveals the mechanisms of Mycena dendrobii promoting transplantation survival and growth of tissue culture seedlings of Dendrobium officinale.
Identifieur interne : 001530 ( Main/Corpus ); précédent : 001529; suivant : 001531Proteomic analysis reveals the mechanisms of Mycena dendrobii promoting transplantation survival and growth of tissue culture seedlings of Dendrobium officinale.
Auteurs : X B Xu ; X Y Ma ; H H Lei ; H M Song ; Q C Ying ; M J Xu ; S B Liu ; H Z WangSource :
- Journal of applied microbiology [ 1365-2672 ] ; 2015.
English descriptors
- KwdEn :
- Agaricales (growth & development), Agaricales (physiology), Dendrobium (chemistry), Dendrobium (growth & development), Dendrobium (metabolism), Dendrobium (microbiology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (chemistry), Plant Roots (growth & development), Plant Roots (metabolism), Plant Roots (microbiology), Proteomics (MeSH), Seedlings (chemistry), Seedlings (growth & development), Seedlings (metabolism), Seedlings (microbiology).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemistry : Dendrobium, Plant Roots, Seedlings.
- chemical , genetics : Plant Proteins.
- growth & development : Agaricales, Dendrobium, Plant Roots, Seedlings.
- metabolism : Dendrobium, Plant Proteins, Plant Roots, Seedlings.
- microbiology : Dendrobium, Plant Roots, Seedlings.
- physiology : Agaricales.
- Proteomics.
Abstract
AIMS
Dendrobium officinale is an important traditional Chinese medicinal herb. Its seedlings generally show low survival and growth when transferred from in vitro tissue culture to a greenhouse or field environment. In this study, the effect of Mycena dendrobii on the survival and growth of D. officinale tissue culture seedlings and the mechanisms involved was explored.
METHODS AND RESULTS
Mycena dendrobii were applied underneath the roots of D. officinale tissue culture seedlings. The seedling survival and growth were analysed. The root proteins induced by M. dendrobii were identified using two-dimensional (2-D) electrophoresis and matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS). Mycena dendrobii treatment significantly enhanced survival and growth of D. officinale seedlings. Forty-one proteins induced by M. dendrobii were identified. Among them, 10 were involved in defence and stress response, two were involved in the formation of root or mycorrhizae, and three were related to the biosynthesis of bioactive constituents.
CONCLUSIONS
These results suggest that enhancing stress tolerance and promoting new root formation induced by M. dendrobii may improve the survival and growth of D. officinale tissue culture seedlings.
SIGNIFICANCE AND IMPACT OF THE STUDY
This study provides a foundation for future use of M. dendrobii in the large-scale cultivation of Dendrobiums.
DOI: 10.1111/jam.12781
PubMed: 25732577
Links to Exploration step
pubmed:25732577Le document en format XML
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, H M" sort="Song, H M" uniqKey="Song H" first="H M" last="Song">H M Song</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ying, Q C" sort="Ying, Q C" uniqKey="Ying Q" first="Q C" last="Ying">Q C Ying</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, M J" sort="Xu, M J" uniqKey="Xu M" first="M J" last="Xu">M J Xu</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, S B" sort="Liu, S B" uniqKey="Liu S" first="S B" last="Liu">S B Liu</name><affiliation><nlm:affiliation>College of Life and Environmental 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sort="Xu, X B" uniqKey="Xu X" first="X B" last="Xu">X B Xu</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, X Y" sort="Ma, X Y" uniqKey="Ma X" first="X Y" last="Ma">X Y Ma</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lei, H H" sort="Lei, H H" uniqKey="Lei H" first="H H" last="Lei">H H Lei</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, H M" sort="Song, H M" uniqKey="Song H" first="H M" last="Song">H M Song</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ying, Q C" sort="Ying, Q C" uniqKey="Ying Q" first="Q C" last="Ying">Q C Ying</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, M J" sort="Xu, M J" uniqKey="Xu M" first="M J" last="Xu">M J Xu</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Liu, S B" sort="Liu, S B" uniqKey="Liu S" first="S B" last="Liu">S B Liu</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, H Z" sort="Wang, H Z" uniqKey="Wang H" first="H Z" last="Wang">H Z Wang</name><affiliation><nlm:affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Journal of applied microbiology</title><idno type="eISSN">1365-2672</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agaricales (growth & development)</term><term>Agaricales (physiology)</term><term>Dendrobium (chemistry)</term><term>Dendrobium (growth & development)</term><term>Dendrobium (metabolism)</term><term>Dendrobium (microbiology)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (chemistry)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (metabolism)</term><term>Plant Roots (microbiology)</term><term>Proteomics (MeSH)</term><term>Seedlings (chemistry)</term><term>Seedlings (growth & development)</term><term>Seedlings (metabolism)</term><term>Seedlings (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Dendrobium</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Agaricales</term><term>Dendrobium</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Dendrobium</term><term>Plant Proteins</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Dendrobium</term><term>Plant Roots</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agaricales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Proteomics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>AIMS</b></p><p>Dendrobium officinale is an important traditional Chinese medicinal herb. Its seedlings generally show low survival and growth when transferred from in vitro tissue culture to a greenhouse or field environment. In this study, the effect of Mycena dendrobii on the survival and growth of D. officinale tissue culture seedlings and the mechanisms involved was explored.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS AND RESULTS</b></p><p>Mycena dendrobii were applied underneath the roots of D. officinale tissue culture seedlings. The seedling survival and growth were analysed. The root proteins induced by M. dendrobii were identified using two-dimensional (2-D) electrophoresis and matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS). Mycena dendrobii treatment significantly enhanced survival and growth of D. officinale seedlings. Forty-one proteins induced by M. dendrobii were identified. Among them, 10 were involved in defence and stress response, two were involved in the formation of root or mycorrhizae, and three were related to the biosynthesis of bioactive constituents.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>These results suggest that enhancing stress tolerance and promoting new root formation induced by M. dendrobii may improve the survival and growth of D. officinale tissue culture seedlings.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>This study provides a foundation for future use of M. dendrobii in the large-scale cultivation of Dendrobiums.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">25732577</PMID><DateCompleted><Year>2016</Year><Month>04</Month><Day>13</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-2672</ISSN><JournalIssue CitedMedium="Internet"><Volume>118</Volume><Issue>6</Issue><PubDate><Year>2015</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of applied microbiology</Title><ISOAbbreviation>J Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Proteomic analysis reveals the mechanisms of Mycena dendrobii promoting transplantation survival and growth of tissue culture seedlings of Dendrobium officinale.</ArticleTitle><Pagination><MedlinePgn>1444-55</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/jam.12781</ELocationID><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Dendrobium officinale is an important traditional Chinese medicinal herb. Its seedlings generally show low survival and growth when transferred from in vitro tissue culture to a greenhouse or field environment. In this study, the effect of Mycena dendrobii on the survival and growth of D. officinale tissue culture seedlings and the mechanisms involved was explored.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">Mycena dendrobii were applied underneath the roots of D. officinale tissue culture seedlings. The seedling survival and growth were analysed. The root proteins induced by M. dendrobii were identified using two-dimensional (2-D) electrophoresis and matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF-MS). Mycena dendrobii treatment significantly enhanced survival and growth of D. officinale seedlings. Forty-one proteins induced by M. dendrobii were identified. Among them, 10 were involved in defence and stress response, two were involved in the formation of root or mycorrhizae, and three were related to the biosynthesis of bioactive constituents.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">These results suggest that enhancing stress tolerance and promoting new root formation induced by M. dendrobii may improve the survival and growth of D. officinale tissue culture seedlings.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">This study provides a foundation for future use of M. dendrobii in the large-scale cultivation of Dendrobiums.</AbstractText><CopyrightInformation>© 2015 The Society for Applied Microbiology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>X B</ForeName><Initials>XB</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>X Y</ForeName><Initials>XY</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lei</LastName><ForeName>H H</ForeName><Initials>HH</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>H M</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ying</LastName><ForeName>Q C</ForeName><Initials>QC</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>M J</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>S B</ForeName><Initials>SB</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>H Z</ForeName><Initials>HZ</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>04</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Appl Microbiol</MedlineTA><NlmUniqueID>9706280</NlmUniqueID><ISSNLinking>1364-5072</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000363" MajorTopicYN="N">Agaricales</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031667" MajorTopicYN="N">Dendrobium</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Dendrobium</Keyword><Keyword MajorTopicYN="N">Mycena dendrobii</Keyword><Keyword MajorTopicYN="N">mycorrhiza</Keyword><Keyword MajorTopicYN="N">proteome</Keyword><Keyword MajorTopicYN="N">transplantation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>11</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>02</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>02</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>3</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25732577</ArticleId><ArticleId IdType="doi">10.1111/jam.12781</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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