Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.
Identifieur interne : 001264 ( Main/Corpus ); précédent : 001263; suivant : 001265Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.
Auteurs : M Sudhakara Reddy ; Manpreet Kour ; Sipla Aggarwal ; Shanky Ahuja ; Roland Marmeisse ; Laurence Fraissinet-TachetSource :
- Environmental microbiology [ 1462-2920 ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Basidiomycota (metabolism), Cadmium (toxicity), Copper (toxicity), Eucalyptus (drug effects), Eucalyptus (microbiology), Metallothionein (metabolism), Mycorrhizae (metabolism), Plant Development (drug effects), Plant Development (physiology), Sequence Alignment (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , metabolism : Metallothionein.
- chemical , toxicity : Cadmium, Copper.
- drug effects : Eucalyptus, Plant Development.
- metabolism : Basidiomycota, Mycorrhizae.
- microbiology : Eucalyptus.
- physiology : Plant Development.
- Amino Acid Sequence, Sequence Alignment, Symbiosis.
Abstract
Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.
DOI: 10.1111/1462-2920.13149
PubMed: 26626627
Links to Exploration step
pubmed:26626627Le document en format XML
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<series><title level="j">Environmental microbiology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Basidiomycota (metabolism)</term>
<term>Cadmium (toxicity)</term>
<term>Copper (toxicity)</term>
<term>Eucalyptus (drug effects)</term>
<term>Eucalyptus (microbiology)</term>
<term>Metallothionein (metabolism)</term>
<term>Mycorrhizae (metabolism)</term>
<term>Plant Development (drug effects)</term>
<term>Plant Development (physiology)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Symbiosis (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Metallothionein</term>
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<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term>
<term>Copper</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Eucalyptus</term>
<term>Plant Development</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term>
<term>Mycorrhizae</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Eucalyptus</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Development</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Sequence Alignment</term>
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<front><div type="abstract" xml:lang="en">Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.</div>
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<Abstract><AbstractText>Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.</AbstractText>
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