Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.
Identifieur interne : 001264 ( Main/Corpus ); précédent : 001263; suivant : 001265Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.
Auteurs : M Sudhakara Reddy ; Manpreet Kour ; Sipla Aggarwal ; Shanky Ahuja ; Roland Marmeisse ; Laurence Fraissinet-TachetSource :
- Environmental microbiology [ 1462-2920 ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Basidiomycota (metabolism), Cadmium (toxicity), Copper (toxicity), Eucalyptus (drug effects), Eucalyptus (microbiology), Metallothionein (metabolism), Mycorrhizae (metabolism), Plant Development (drug effects), Plant Development (physiology), Sequence Alignment (MeSH), Symbiosis (MeSH).
- MESH :
- chemical , metabolism : Metallothionein.
- chemical , toxicity : Cadmium, Copper.
- drug effects : Eucalyptus, Plant Development.
- metabolism : Basidiomycota, Mycorrhizae.
- microbiology : Eucalyptus.
- physiology : Plant Development.
- Amino Acid Sequence, Sequence Alignment, Symbiosis.
Abstract
Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.
DOI: 10.1111/1462-2920.13149
PubMed: 26626627
Links to Exploration step
pubmed:26626627Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.</title><author><name sortKey="Reddy, M Sudhakara" sort="Reddy, M Sudhakara" uniqKey="Reddy M" first="M Sudhakara" last="Reddy">M Sudhakara Reddy</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Kour, Manpreet" sort="Kour, Manpreet" uniqKey="Kour M" first="Manpreet" last="Kour">Manpreet Kour</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Aggarwal, Sipla" sort="Aggarwal, Sipla" uniqKey="Aggarwal S" first="Sipla" last="Aggarwal">Sipla Aggarwal</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Ahuja, Shanky" sort="Ahuja, Shanky" uniqKey="Ahuja S" first="Shanky" last="Ahuja">Shanky Ahuja</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name><affiliation><nlm:affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name><affiliation><nlm:affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26626627</idno><idno type="pmid">26626627</idno><idno type="doi">10.1111/1462-2920.13149</idno><idno type="wicri:Area/Main/Corpus">001264</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001264</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.</title><author><name sortKey="Reddy, M Sudhakara" sort="Reddy, M Sudhakara" uniqKey="Reddy M" first="M Sudhakara" last="Reddy">M Sudhakara Reddy</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Kour, Manpreet" sort="Kour, Manpreet" uniqKey="Kour M" first="Manpreet" last="Kour">Manpreet Kour</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Aggarwal, Sipla" sort="Aggarwal, Sipla" uniqKey="Aggarwal S" first="Sipla" last="Aggarwal">Sipla Aggarwal</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Ahuja, Shanky" sort="Ahuja, Shanky" uniqKey="Ahuja S" first="Shanky" last="Ahuja">Shanky Ahuja</name><affiliation><nlm:affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</nlm:affiliation></affiliation></author><author><name sortKey="Marmeisse, Roland" sort="Marmeisse, Roland" uniqKey="Marmeisse R" first="Roland" last="Marmeisse">Roland Marmeisse</name><affiliation><nlm:affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</nlm:affiliation></affiliation></author><author><name sortKey="Fraissinet Tachet, Laurence" sort="Fraissinet Tachet, Laurence" uniqKey="Fraissinet Tachet L" first="Laurence" last="Fraissinet-Tachet">Laurence Fraissinet-Tachet</name><affiliation><nlm:affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental microbiology</title><idno type="eISSN">1462-2920</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Basidiomycota (metabolism)</term><term>Cadmium (toxicity)</term><term>Copper (toxicity)</term><term>Eucalyptus (drug effects)</term><term>Eucalyptus (microbiology)</term><term>Metallothionein (metabolism)</term><term>Mycorrhizae (metabolism)</term><term>Plant Development (drug effects)</term><term>Plant Development (physiology)</term><term>Sequence Alignment (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Metallothionein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term><term>Copper</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Eucalyptus</term><term>Plant Development</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Eucalyptus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Development</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Sequence Alignment</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">26626627</PMID><DateCompleted><Year>2017</Year><Month>09</Month><Day>07</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-2920</ISSN><JournalIssue CitedMedium="Internet"><Volume>18</Volume><Issue>8</Issue><PubDate><Year>2016</Year><Month>09</Month></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.</ArticleTitle><Pagination><MedlinePgn>2446-54</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1462-2920.13149</ELocationID><Abstract><AbstractText>Metallothioneins (MTs) are small, cysteine-rich peptides involved in intracellular sequestration of heavy metals in eukaryotes. We examined the role in metal homeostasis and detoxification of an MT from the ectomycorrhizal fungus Pisolithus albus (PaMT1). PaMT1 encodes a 35 amino acid-long polypeptide, with 7 cysteine residues; most of them part of a C-x-C motif found in other known basidiomycete MTs. The expression levels of PaMT1 increased as a function of increased external Cu and Cd concentrations and were higher with Cu than with Cd. Heterologous complementation assays in metal-sensitive yeast mutants indicated that PaMT1 encodes a polypeptide capable of conferring higher tolerance to both Cu and Cd. Eucalyptus tereticornis plantlets colonized with P. albus grown in the presence of Cu and Cd showed better growth compared with those with non-mycorrhizal plants. Higher PaMT1 expression levels were recorded in mycorrhizal plants grown in the presence of Cu and Cd compared with those in control mycorrhizal plants not exposed to heavy metals. These data provide the first evidence to our knowledge that fungal MTs could protect ectomycorrhizal fungi from heavy metal stress and in turn help the plants to establish in metal-contaminated sites.</AbstractText><CopyrightInformation>© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Reddy</LastName><ForeName>M Sudhakara</ForeName><Initials>MS</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kour</LastName><ForeName>Manpreet</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Aggarwal</LastName><ForeName>Sipla</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahuja</LastName><ForeName>Shanky</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Thapar University, Bhadson Road, Patiala, 147 004, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Marmeisse</LastName><ForeName>Roland</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fraissinet-Tachet</LastName><ForeName>Laurence</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Microbial Ecology, CNRS UMR 5557, USC INRA 1364, Université Lyon1, Université de Lyon, F-69622, Villeurbanne, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AAS19463</AccessionNumber><AccessionNumber>ABP02008</AccessionNumber><AccessionNumber>ACT83730</AccessionNumber><AccessionNumber>AF440868</AccessionNumber><AccessionNumber>AGO04615</AccessionNumber><AccessionNumber>AHA31882</AccessionNumber><AccessionNumber>AJO67962</AccessionNumber><AccessionNumber>BF942677</AccessionNumber><AccessionNumber>CO501612</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>01</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>789U1901C5</RegistryNumber><NameOfSubstance UI="D003300">Copper</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003300" MajorTopicYN="N">Copper</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005052" MajorTopicYN="N">Eucalyptus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D063245" MajorTopicYN="N">Plant Development</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2015</Year><Month>11</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2015</Year><Month>11</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>12</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2017</Year><Month>9</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26626627</ArticleId><ArticleId IdType="doi">10.1111/1462-2920.13149</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MycorrhizaeV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001264 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 001264 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MycorrhizaeV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:26626627
|texte= Metal induction of a Pisolithus albus metallothionein and its potential involvement in heavy metal tolerance during mycorrhizal symbiosis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:26626627" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MycorrhizaeV1
| This area was generated with Dilib version V0.6.37. |  |