Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings.
Identifieur interne : 001174 ( Main/Corpus ); précédent : 001173; suivant : 001175Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings.
Auteurs : Munemasa Teramoto ; Bingyun Wu ; Taizo HogetsuSource :
- Mycorrhiza [ 1432-1890 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical : Carbon Radioisotopes.
- metabolism : Pinus, Seedlings.
- physiology : Basidiomycota, Mycorrhizae, Photosynthesis, Symbiosis.
- Autoradiography.
Abstract
The purpose of this study was to identify the pathway and sink activity of photosynthate translocation in the extraradical mycelium (ERM) of a Pisolithus isolate. We labelled ectomycorrhizal (ECM) Pinus thunbergii seedlings with (14)CO2 and followed (14)C distribution within the ERM by autoradiography. (14)C photosynthate translocation in the ERM resulted in (14)C distribution in rhizomorphs throughout the ERM, with (14)C accumulation at the front. When most radial mycelial connections between ECM root tips and the ERM front were cut, the whole allocation of (14)C photosynthates to the ERM was reduced. However, the overall pattern of (14)C distribution in the ERM was maintained even in regions immediately above and below the cut, with no local (14)C depletion or accumulation. We inferred from this result that every portion in the ERM has a significant sink activity and a definite sink capacity for photosynthates and that photosynthates detour the cut and reach throughout the ERM by translocation in every direction. Next, we prepared paired ECM seedlings, ERMs of which had been connected with each other by hyphal fusion, alongside, labelled the left seedling with (14)CO2, and shaded none, one or both of them. (14)C photosynthates were acropetally and basipetally translocated from the left ERM to ECM root tips of the right seedling through rhizomorphs in the left and right ERMs, respectively. With the left seedling illuminated, (14)C translocation from the left to the right ERM increased by shading the right seedling. This result suggests that reduced photosynthate transfer from the host to its ERM increased sink activity of the ERM.
DOI: 10.1007/s00572-016-0684-5
PubMed: 26861479
Links to Exploration step
pubmed:26861479Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings.</title><author><name sortKey="Teramoto, Munemasa" sort="Teramoto, Munemasa" uniqKey="Teramoto M" first="Munemasa" last="Teramoto">Munemasa Teramoto</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan. mthope007@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Present address: Center for Global Environmental Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, 305-8506, Japan. mthope007@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Bingyun" sort="Wu, Bingyun" uniqKey="Wu B" first="Bingyun" last="Wu">Bingyun Wu</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hogetsu, Taizo" sort="Hogetsu, Taizo" uniqKey="Hogetsu T" first="Taizo" last="Hogetsu">Taizo Hogetsu</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26861479</idno><idno type="pmid">26861479</idno><idno type="doi">10.1007/s00572-016-0684-5</idno><idno type="wicri:Area/Main/Corpus">001174</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001174</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings.</title><author><name sortKey="Teramoto, Munemasa" sort="Teramoto, Munemasa" uniqKey="Teramoto M" first="Munemasa" last="Teramoto">Munemasa Teramoto</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan. mthope007@gmail.com.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Present address: Center for Global Environmental Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, 305-8506, Japan. mthope007@gmail.com.</nlm:affiliation></affiliation></author><author><name sortKey="Wu, Bingyun" sort="Wu, Bingyun" uniqKey="Wu B" first="Bingyun" last="Wu">Bingyun Wu</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Hogetsu, Taizo" sort="Hogetsu, Taizo" uniqKey="Hogetsu T" first="Taizo" last="Hogetsu">Taizo Hogetsu</name><affiliation><nlm:affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autoradiography (MeSH)</term><term>Basidiomycota (physiology)</term><term>Carbon Radioisotopes (MeSH)</term><term>Mycorrhizae (physiology)</term><term>Photosynthesis (physiology)</term><term>Pinus (metabolism)</term><term>Seedlings (metabolism)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Carbon Radioisotopes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pinus</term><term>Seedlings</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term><term>Mycorrhizae</term><term>Photosynthesis</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autoradiography</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The purpose of this study was to identify the pathway and sink activity of photosynthate translocation in the extraradical mycelium (ERM) of a Pisolithus isolate. We labelled ectomycorrhizal (ECM) Pinus thunbergii seedlings with (14)CO2 and followed (14)C distribution within the ERM by autoradiography. (14)C photosynthate translocation in the ERM resulted in (14)C distribution in rhizomorphs throughout the ERM, with (14)C accumulation at the front. When most radial mycelial connections between ECM root tips and the ERM front were cut, the whole allocation of (14)C photosynthates to the ERM was reduced. However, the overall pattern of (14)C distribution in the ERM was maintained even in regions immediately above and below the cut, with no local (14)C depletion or accumulation. We inferred from this result that every portion in the ERM has a significant sink activity and a definite sink capacity for photosynthates and that photosynthates detour the cut and reach throughout the ERM by translocation in every direction. Next, we prepared paired ECM seedlings, ERMs of which had been connected with each other by hyphal fusion, alongside, labelled the left seedling with (14)CO2, and shaded none, one or both of them. (14)C photosynthates were acropetally and basipetally translocated from the left ERM to ECM root tips of the right seedling through rhizomorphs in the left and right ERMs, respectively. With the left seedling illuminated, (14)C translocation from the left to the right ERM increased by shading the right seedling. This result suggests that reduced photosynthate transfer from the host to its ERM increased sink activity of the ERM. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26861479</PMID><DateCompleted><Year>2017</Year><Month>02</Month><Day>21</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>26</Volume><Issue>5</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Pathway and sink activity for photosynthate translocation in Pisolithus extraradical mycelium of ectomycorrhizal Pinus thunbergii seedlings.</ArticleTitle><Pagination><MedlinePgn>453-64</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-016-0684-5</ELocationID><Abstract><AbstractText>The purpose of this study was to identify the pathway and sink activity of photosynthate translocation in the extraradical mycelium (ERM) of a Pisolithus isolate. We labelled ectomycorrhizal (ECM) Pinus thunbergii seedlings with (14)CO2 and followed (14)C distribution within the ERM by autoradiography. (14)C photosynthate translocation in the ERM resulted in (14)C distribution in rhizomorphs throughout the ERM, with (14)C accumulation at the front. When most radial mycelial connections between ECM root tips and the ERM front were cut, the whole allocation of (14)C photosynthates to the ERM was reduced. However, the overall pattern of (14)C distribution in the ERM was maintained even in regions immediately above and below the cut, with no local (14)C depletion or accumulation. We inferred from this result that every portion in the ERM has a significant sink activity and a definite sink capacity for photosynthates and that photosynthates detour the cut and reach throughout the ERM by translocation in every direction. Next, we prepared paired ECM seedlings, ERMs of which had been connected with each other by hyphal fusion, alongside, labelled the left seedling with (14)CO2, and shaded none, one or both of them. (14)C photosynthates were acropetally and basipetally translocated from the left ERM to ECM root tips of the right seedling through rhizomorphs in the left and right ERMs, respectively. With the left seedling illuminated, (14)C translocation from the left to the right ERM increased by shading the right seedling. This result suggests that reduced photosynthate transfer from the host to its ERM increased sink activity of the ERM. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Teramoto</LastName><ForeName>Munemasa</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan. mthope007@gmail.com.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Present address: Center for Global Environmental Research, National Institute for Environmental Studies, Onogawa 16-2, Tsukuba, 305-8506, Japan. mthope007@gmail.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Bingyun</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hogetsu</LastName><ForeName>Taizo</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Department of Forest Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>02</Month><Day>09</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002250">Carbon 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MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D036226" MajorTopicYN="N">Seedlings</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Acropetal and basipetal</Keyword><Keyword MajorTopicYN="N">Common mycelial network</Keyword><Keyword MajorTopicYN="N">Ectomycorrhizal symbiosis</Keyword><Keyword MajorTopicYN="N">Extramatrical mycelium</Keyword><Keyword MajorTopicYN="N">Soil nutrients</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year><Month>08</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>01</Month><Day>29</Day></PubMedPubDate><PubMedPubDate 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