Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP.
Identifieur interne : 001008 ( Main/Corpus ); précédent : 001007; suivant : 001009Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP.
Auteurs : Shintaro Hara ; Masanori SaitoSource :
- Microbes and environments [ 1347-4405 ] ; 2016.
English descriptors
- KwdEn :
- 6-Phytase (genetics), Alginates (MeSH), Bacteria (isolation & purification), Bacteria (metabolism), Bacteriological Techniques (methods), Biocompatible Materials (MeSH), Biodiversity (MeSH), Biotransformation (MeSH), Caulobacter (MeSH), Cluster Analysis (MeSH), DNA, Bacterial (chemistry), DNA, Bacterial (genetics), DNA, Ribosomal (chemistry), DNA, Ribosomal (genetics), Denaturing Gradient Gel Electrophoresis (MeSH), Glucuronic Acid (MeSH), Hexuronic Acids (MeSH), Mycorrhizae (MeSH), Phylogeny (MeSH), Phytic Acid (metabolism), RNA, Ribosomal, 16S (genetics), Sequence Analysis, DNA (MeSH), Sphingomonas (MeSH).
- MESH :
- chemical , chemistry : DNA, Bacterial, DNA, Ribosomal.
- chemical , genetics : 6-Phytase, DNA, Bacterial, DNA, Ribosomal, RNA, Ribosomal, 16S.
- chemical , metabolism : Phytic Acid.
- chemical : Alginates, Biocompatible Materials, Glucuronic Acid, Hexuronic Acids.
- isolation & purification : Bacteria.
- metabolism : Bacteria.
- methods : Bacteriological Techniques.
- Biodiversity, Biotransformation, Caulobacter, Cluster Analysis, Denaturing Gradient Gel Electrophoresis, Mycorrhizae, Phylogeny, Sequence Analysis, DNA, Sphingomonas.
Abstract
Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF.
DOI: 10.1264/jsme2.ME15206
PubMed: 27383681
PubMed Central: PMC5017799
Links to Exploration step
pubmed:27383681Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP.</title><author><name sortKey="Hara, Shintaro" sort="Hara, Shintaro" uniqKey="Hara S" first="Shintaro" last="Hara">Shintaro Hara</name><affiliation><nlm:affiliation>Graduate School of Agricultural Science, Tohoku University.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, Masanori" sort="Saito, Masanori" uniqKey="Saito M" first="Masanori" last="Saito">Masanori Saito</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27383681</idno><idno type="pmid">27383681</idno><idno type="doi">10.1264/jsme2.ME15206</idno><idno type="pmc">PMC5017799</idno><idno type="wicri:Area/Main/Corpus">001008</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001008</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP.</title><author><name sortKey="Hara, Shintaro" sort="Hara, Shintaro" uniqKey="Hara S" first="Shintaro" last="Hara">Shintaro Hara</name><affiliation><nlm:affiliation>Graduate School of Agricultural Science, Tohoku University.</nlm:affiliation></affiliation></author><author><name sortKey="Saito, Masanori" sort="Saito, Masanori" uniqKey="Saito M" first="Masanori" last="Saito">Masanori Saito</name></author></analytic><series><title level="j">Microbes and environments</title><idno type="eISSN">1347-4405</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>6-Phytase (genetics)</term><term>Alginates (MeSH)</term><term>Bacteria (isolation & purification)</term><term>Bacteria (metabolism)</term><term>Bacteriological Techniques (methods)</term><term>Biocompatible Materials (MeSH)</term><term>Biodiversity (MeSH)</term><term>Biotransformation (MeSH)</term><term>Caulobacter (MeSH)</term><term>Cluster Analysis (MeSH)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (genetics)</term><term>DNA, Ribosomal (chemistry)</term><term>DNA, Ribosomal (genetics)</term><term>Denaturing Gradient Gel Electrophoresis (MeSH)</term><term>Glucuronic Acid (MeSH)</term><term>Hexuronic Acids (MeSH)</term><term>Mycorrhizae (MeSH)</term><term>Phylogeny (MeSH)</term><term>Phytic Acid (metabolism)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sphingomonas (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>6-Phytase</term><term>DNA, Bacterial</term><term>DNA, Ribosomal</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Phytic Acid</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Alginates</term><term>Biocompatible Materials</term><term>Glucuronic Acid</term><term>Hexuronic Acids</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Bacteriological Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Biotransformation</term><term>Caulobacter</term><term>Cluster Analysis</term><term>Denaturing Gradient Gel Electrophoresis</term><term>Mycorrhizae</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Sphingomonas</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27383681</PMID><DateCompleted><Year>2017</Year><Month>03</Month><Day>27</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4405</ISSN><JournalIssue CitedMedium="Internet"><Volume>31</Volume><Issue>3</Issue><PubDate><Year>2016</Year><Month>Sep</Month><Day>29</Day></PubDate></JournalIssue><Title>Microbes and environments</Title><ISOAbbreviation>Microbes Environ</ISOAbbreviation></Journal><ArticleTitle>Isolation of Inositol Hexaphosphate (IHP)-Degrading Bacteria from Arbuscular Mycorrhizal Fungal Hyphal Compartments Using a Modified Baiting Method Involving Alginate Beads Containing IHP.</ArticleTitle><Pagination><MedlinePgn>234-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1264/jsme2.ME15206</ELocationID><Abstract><AbstractText>Phytate (inositol hexaphosphate; IHP)-degrading microbes have been suggested to contribute to arbuscular mycorrhizal fungi (AMF)-mediated P transfer from IHP to plants; however, no IHP degrader involved in AMF-mediated P transfer has been isolated to date. We herein report the isolation of IHP-degrading bacteria using a modified baiting method. We applied alginate beads as carriers of IHP powder, and used them as recoverable IHP in the AM fungal compartment of plant cultivation experiments. P transfer from IHP in alginate beads via AMF was confirmed, and extracted DNA from alginate beads was analyzed by denaturing gradient gel electrophoresis targeting the 16S rRNA gene and a clone library method for the beta-propeller phytase (BPP) gene. The diversities of the 16S rRNA and BPP genes of microbes growing on IHP beads were simple and those of Sphingomonas spp. and Caulobacter spp. dominated. A total of 187 IHP-utilizing bacteria were isolated and identified, and they were consistent with the results of DNA analysis. Furthermore, some isolated Sphingomonas spp. and Caulobacter sp. showed IHP-degrading activity. Therefore, we successfully isolated dominant IHP-degrading bacteria from IHP in an AMF hyphal compartment. These strains may contribute to P transfer from IHP via AMF. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hara</LastName><ForeName>Shintaro</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Graduate School of Agricultural Science, Tohoku University.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saito</LastName><ForeName>Masanori</ForeName><Initials>M</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>07</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Microbes Environ</MedlineTA><NlmUniqueID>9710937</NlmUniqueID><ISSNLinking>1342-6311</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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