The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi.
Identifieur interne : 000985 ( Main/Corpus ); précédent : 000984; suivant : 000986The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi.
Auteurs : Massimo Turina ; Stefano Ghignone ; Nausicaa Astolfi ; Alessandro Silvestri ; Paola Bonfante ; Luisa LanfrancoSource :
- Environmental microbiology [ 1462-2920 ] ; 2018.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : DNA, Viral.
- genetics : Fungal Viruses, Fungi, Glomeromycota, Mycorrhizae, RNA Viruses.
- isolation & purification : RNA Viruses.
- virology : Glomeromycota, Mycorrhizae.
Abstract
Arbuscular Mycorrhizal Fungi (AMF) are key components of the plant microbiota. AMF genetic complexity is increased by the presence of endobacteria, which live inside many species. A further component of such complexity is the virome associated to AMF, whose knowledge is still very limited. Here, by exploiting transcriptomic data we describe the virome of Gigaspora margarita. A BLAST search for viral RNA-dependent RNA polymerases sequences allowed the identification of four mitoviruses, one Ourmia-like narnavirus, one Giardia-like virus, and two sequences related to Fusarium graminearum mycoviruses. Northern blot and RT-PCR confirmed the authenticity of all the sequences with the exception of the F. graminearum-related ones. All the mitoviruses are replicative and functional since both positive strand and negative strand RNA are present. The abundance of the viral RNA molecules is not regulated by the presence or absence of Candidatus Glomeribacter gigasporarum, the endobacterium hosted by G. margarita, with the exception of the Ourmia-like sequence which is absent in bacteria-cured spores. In addition, we report, for the first time, DNA fragments corresponding to mitovirus sequences associated to the presence of viral RNA. These sequences are not integrated in the mitochondrial DNA and preliminary evidence seems to exclude integration in the nuclear genome.
DOI: 10.1111/1462-2920.14060
PubMed: 29393558
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pubmed:29393558Le document en format XML
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Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Silvestri, Alessandro" sort="Silvestri, Alessandro" uniqKey="Silvestri A" first="Alessandro" last="Silvestri">Alessandro Silvestri</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Lanfranco, Luisa" sort="Lanfranco, Luisa" uniqKey="Lanfranco L" first="Luisa" last="Lanfranco">Luisa Lanfranco</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29393558</idno><idno type="pmid">29393558</idno><idno type="doi">10.1111/1462-2920.14060</idno><idno type="wicri:Area/Main/Corpus">000985</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000985</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi.</title><author><name sortKey="Turina, Massimo" sort="Turina, Massimo" uniqKey="Turina M" first="Massimo" last="Turina">Massimo Turina</name><affiliation><nlm:affiliation>Institute for Sustainable Plant Protection - CNR Torino, Strada delle Cacce 73, 10131 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Ghignone, Stefano" sort="Ghignone, Stefano" uniqKey="Ghignone S" first="Stefano" last="Ghignone">Stefano Ghignone</name><affiliation><nlm:affiliation>Institute for Sustainable Plant Protection - CNR Torino, Strada delle Cacce 73, 10131 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Astolfi, Nausicaa" sort="Astolfi, Nausicaa" uniqKey="Astolfi N" first="Nausicaa" last="Astolfi">Nausicaa Astolfi</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Silvestri, Alessandro" sort="Silvestri, Alessandro" uniqKey="Silvestri A" first="Alessandro" last="Silvestri">Alessandro Silvestri</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Bonfante, Paola" sort="Bonfante, Paola" uniqKey="Bonfante P" first="Paola" last="Bonfante">Paola Bonfante</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Lanfranco, Luisa" sort="Lanfranco, Luisa" uniqKey="Lanfranco L" first="Luisa" last="Lanfranco">Luisa Lanfranco</name><affiliation><nlm:affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Environmental microbiology</title><idno type="eISSN">1462-2920</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Viral (isolation & purification)</term><term>Fungal Viruses (genetics)</term><term>Fungi (genetics)</term><term>Glomeromycota (genetics)</term><term>Glomeromycota (virology)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (virology)</term><term>RNA Viruses (genetics)</term><term>RNA Viruses (isolation & purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungal Viruses</term><term>Fungi</term><term>Glomeromycota</term><term>Mycorrhizae</term><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular Mycorrhizal Fungi (AMF) are key components of the plant microbiota. AMF genetic complexity is increased by the presence of endobacteria, which live inside many species. A further component of such complexity is the virome associated to AMF, whose knowledge is still very limited. Here, by exploiting transcriptomic data we describe the virome of Gigaspora margarita. A BLAST search for viral RNA-dependent RNA polymerases sequences allowed the identification of four mitoviruses, one Ourmia-like narnavirus, one Giardia-like virus, and two sequences related to Fusarium graminearum mycoviruses. Northern blot and RT-PCR confirmed the authenticity of all the sequences with the exception of the F. graminearum-related ones. All the mitoviruses are replicative and functional since both positive strand and negative strand RNA are present. The abundance of the viral RNA molecules is not regulated by the presence or absence of Candidatus Glomeribacter gigasporarum, the endobacterium hosted by G. margarita, with the exception of the Ourmia-like sequence which is absent in bacteria-cured spores. In addition, we report, for the first time, DNA fragments corresponding to mitovirus sequences associated to the presence of viral RNA. These sequences are not integrated in the mitochondrial DNA and preliminary evidence seems to exclude integration in the nuclear genome.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">29393558</PMID><DateCompleted><Year>2019</Year><Month>09</Month><Day>23</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>23</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1462-2920</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>6</Issue><PubDate><Year>2018</Year><Month>06</Month></PubDate></JournalIssue><Title>Environmental microbiology</Title><ISOAbbreviation>Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>The virome of the arbuscular mycorrhizal fungus Gigaspora margarita reveals the first report of DNA fragments corresponding to replicating non-retroviral RNA viruses in fungi.</ArticleTitle><Pagination><MedlinePgn>2012-2025</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1462-2920.14060</ELocationID><Abstract><AbstractText>Arbuscular Mycorrhizal Fungi (AMF) are key components of the plant microbiota. AMF genetic complexity is increased by the presence of endobacteria, which live inside many species. A further component of such complexity is the virome associated to AMF, whose knowledge is still very limited. Here, by exploiting transcriptomic data we describe the virome of Gigaspora margarita. A BLAST search for viral RNA-dependent RNA polymerases sequences allowed the identification of four mitoviruses, one Ourmia-like narnavirus, one Giardia-like virus, and two sequences related to Fusarium graminearum mycoviruses. Northern blot and RT-PCR confirmed the authenticity of all the sequences with the exception of the F. graminearum-related ones. All the mitoviruses are replicative and functional since both positive strand and negative strand RNA are present. The abundance of the viral RNA molecules is not regulated by the presence or absence of Candidatus Glomeribacter gigasporarum, the endobacterium hosted by G. margarita, with the exception of the Ourmia-like sequence which is absent in bacteria-cured spores. In addition, we report, for the first time, DNA fragments corresponding to mitovirus sequences associated to the presence of viral RNA. These sequences are not integrated in the mitochondrial DNA and preliminary evidence seems to exclude integration in the nuclear genome.</AbstractText><CopyrightInformation>© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Turina</LastName><ForeName>Massimo</ForeName><Initials>M</Initials><Identifier Source="ORCID">0000-0002-9659-9470</Identifier><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection - CNR Torino, Strada delle Cacce 73, 10131 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ghignone</LastName><ForeName>Stefano</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-2033-2286</Identifier><AffiliationInfo><Affiliation>Institute for Sustainable Plant Protection - CNR Torino, Strada delle Cacce 73, 10131 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Astolfi</LastName><ForeName>Nausicaa</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silvestri</LastName><ForeName>Alessandro</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bonfante</LastName><ForeName>Paola</ForeName><Initials>P</Initials><Identifier Source="ORCID">0000-0003-3576-8530</Identifier><AffiliationInfo><Affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lanfranco</LastName><ForeName>Luisa</ForeName><Initials>L</Initials><Identifier Source="ORCID">0000-0002-3961-2552</Identifier><AffiliationInfo><Affiliation>Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli 25, 10125 Torino, Italy.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>60% Project</GrantID><Agency>University of Torino</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Environ Microbiol</MedlineTA><NlmUniqueID>100883692</NlmUniqueID><ISSNLinking>1462-2912</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000067148" MajorTopicYN="N">Fungal Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012328" MajorTopicYN="N">RNA Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>01</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>01</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>9</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>2</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29393558</ArticleId><ArticleId IdType="doi">10.1111/1462-2920.14060</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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