Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants.
Identifieur interne : 000741 ( Main/Corpus ); précédent : 000740; suivant : 000742Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants.
Auteurs : Francisco Arenas ; Alfonso Navarro-R Denas ; Daniel Chávez ; Almudena Gutiérrez ; Manuela Pérez-Gilabert ; Asunci N MorteSource :
- Mycorrhiza [ 1432-1890 ] ; 2018.
English descriptors
- KwdEn :
- MESH :
- growth & development : Ascomycota, Mycelium, Mycorrhizae.
- microbiology : Cistaceae, Plant Roots.
- physiology : Ascomycota, Cistaceae, Mycelium, Mycorrhizae, Plant Roots.
Abstract
Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L-1 dry weight and productivity increased from 10.7 to 95.8 mg L-1 day-1 dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.
DOI: 10.1007/s00572-018-0867-3
PubMed: 30238152
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pubmed:30238152Le document en format XML
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Arenas</name><affiliation><nlm:affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Navarro R Denas, Alfonso" sort="Navarro R Denas, Alfonso" uniqKey="Navarro R Denas A" first="Alfonso" last="Navarro-R Denas">Alfonso Navarro-R Denas</name><affiliation><nlm:affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Chavez, Daniel" sort="Chavez, Daniel" uniqKey="Chavez D" first="Daniel" last="Chávez">Daniel Chávez</name><affiliation><nlm:affiliation>Departamento de Ciencias y Tecnología Vegetal, Campus Los Ángeles, Universidad de Concepción, Escuela de Ciencias y Tecnologías, Los Angeles, Chile.</nlm:affiliation></affiliation></author><author><name sortKey="Gutierrez, Almudena" sort="Gutierrez, Almudena" uniqKey="Gutierrez A" first="Almudena" last="Gutiérrez">Almudena Gutiérrez</name><affiliation><nlm:affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Perez Gilabert, Manuela" sort="Perez Gilabert, Manuela" uniqKey="Perez Gilabert M" first="Manuela" last="Pérez-Gilabert">Manuela Pérez-Gilabert</name><affiliation><nlm:affiliation>Departamento de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, Campues de Espinardo, 30100, Murcia, Spain.</nlm:affiliation></affiliation></author><author><name sortKey="Morte, Asunci N" sort="Morte, Asunci N" uniqKey="Morte A" first="Asunci N" last="Morte">Asunci N Morte</name><affiliation><nlm:affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain. amorte@um.es.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="eISSN">1432-1890</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (growth & development)</term><term>Ascomycota (physiology)</term><term>Cistaceae (microbiology)</term><term>Cistaceae (physiology)</term><term>Mycelium (growth & development)</term><term>Mycelium (physiology)</term><term>Mycorrhizae (growth & development)</term><term>Mycorrhizae (physiology)</term><term>Plant Roots (microbiology)</term><term>Plant Roots (physiology)</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Ascomycota</term><term>Mycelium</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Cistaceae</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Ascomycota</term><term>Cistaceae</term><term>Mycelium</term><term>Mycorrhizae</term><term>Plant Roots</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L<sup>-1</sup> dry weight and productivity increased from 10.7 to 95.8 mg L<sup>-1</sup> day<sup>-1</sup> dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30238152</PMID><DateCompleted><Year>2018</Year><Month>12</Month><Day>11</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>11</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-1890</ISSN><JournalIssue CitedMedium="Internet"><Volume>28</Volume><Issue>7</Issue><PubDate><Year>2018</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>Mycelium of Terfezia claveryi as inoculum source to produce desert truffle mycorrhizal plants.</ArticleTitle><Pagination><MedlinePgn>691-701</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-018-0867-3</ELocationID><Abstract><AbstractText>Terfezia claveryi Chatin was the first desert truffle species to be cultivated, the mycorrhizal plants being successfully produced by using both desert truffle spores and mycelia. However, it is more advisable to use mycelium than spores whenever possible and profitable. Given the low yields of mycelia obtained using traditional culture methods of this truffle, the medium composition was modified in an attempt to determine its nutritional requirements. For this, an assay involving response surface methodology was performed using Box-Behnken design to find the optimal parameters for the high production of mycelial biomass. The best results were obtained with glucose as carbon source, buffering the pH at 5 during culture, adding a pool of vitamins, and adjusting the optimal concentrations of carbon and nitrogen sources of the MMN medium. Biomass production increased from 0.3 to 3 g L<sup>-1</sup> dry weight and productivity increased from 10.7 to 95.8 mg L<sup>-1</sup> day<sup>-1</sup> dry weight. The produced mycelium was able to colonize Helianthemum roots efficiently, providing more than 50% ectomycorrhizal colonization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Arenas</LastName><ForeName>Francisco</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Navarro-Ródenas</LastName><ForeName>Alfonso</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chávez</LastName><ForeName>Daniel</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Departamento de Ciencias y Tecnología Vegetal, Campus Los Ángeles, Universidad de Concepción, Escuela de Ciencias y Tecnologías, Los Angeles, Chile.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gutiérrez</LastName><ForeName>Almudena</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 30100, Murcia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pérez-Gilabert</LastName><ForeName>Manuela</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Bioquímica y Biología Molecular A, Facultad de Veterinaria, Universidad de Murcia, Campues de Espinardo, 30100, Murcia, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morte</LastName><ForeName>Asunción</ForeName><Initials>A</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-6426-0202</Identifier><AffiliationInfo><Affiliation>Departamento de Biología Vegetal, Facultad de Biología, Universidad de Murcia, Campus de Espinardo, 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