Transcriptomic analysis reveals the possible roles of sugar metabolism and export for positive mycorrhizal growth responses in soybean.
Identifieur interne : 000723 ( Main/Corpus ); précédent : 000722; suivant : 000724Transcriptomic analysis reveals the possible roles of sugar metabolism and export for positive mycorrhizal growth responses in soybean.
Auteurs : Shaopeng Zhao ; A. Chen ; Chengjie Chen ; Chengchen Li ; Rui Xia ; Xiurong WangSource :
- Physiologia plantarum [ 1399-3054 ] ; 2019.
English descriptors
- KwdEn :
- MESH :
- genetics : Gene Expression Regulation, Plant, Plant Roots, Soybeans, Symbiosis.
- methods : Gene Expression Profiling.
- microbiology : Plant Roots, Soybeans.
- physiology : Mycorrhizae, Symbiosis.
- Sequence Analysis, RNA.
Abstract
To elucidate molecular mechanisms controlling differential growth responses to root colonization by arbuscular mycorrhizal (AM) fungi varying in colonization and cooperative behavior, a pot experiment was carried out using two soybean genotypes and three AM inocula. The results showed that inoculation by cooperative Rhizophagus irregularis (Ri) or less cooperative Glomus aggregatum with high AM colonization (Ga-H) significantly promoted plant growth compared with inoculation by G. aggregatum with low AM colonization (Ga-L). A comparative RNA sequencing analysis of the root transcriptomes showed that fatty acid synthesis pathway was significantly enriched in all three AM inoculation roots. However, sugar metabolism and transport were significantly enriched only in Ri and Ga-H inoculation, which was consistent with positive growth responses in these two inoculation treatments. Accordingly, the expression levels of the key genes related to sugar metabolism and transport were also upregulated in Ri and Ga-H roots compared with Ga-L roots. Of them, two sugars will eventually be exported transporters (SWEET) transporter genes, GmSWEET6 (Glyma.04G198600) and GmSWEET15 (Glyma.06G166800), and one invertase (Glyma.17G227900) gene were exclusively induced only in Ri and Ga-H roots. Promoter analyses in transgenic soybean roots further demonstrated that GUS driven by the GmSWEET6 promoter was highly expressed in arbuscule-containing cortical cells. Additionally, Ri and Ga-H inoculation increased the contents of sucrose, glucose and fructose in both shoots and roots compared with those of Ga-L and non-mycorrhizal. These results imply that positive mycorrhizal growth responses in plants might mostly be due to the stimulation of photosynthate metabolism and transport by AM fungal inoculum with high colonization capabilities.
DOI: 10.1111/ppl.12847
PubMed: 30288747
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Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Chengjie" sort="Chen, Chengjie" uniqKey="Chen C" first="Chengjie" last="Chen">Chengjie Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Chengchen" sort="Li, Chengchen" uniqKey="Li C" first="Chengchen" last="Li">Chengchen Li</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xia, Rui" sort="Xia, Rui" uniqKey="Xia R" first="Rui" last="Xia">Rui Xia</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xiurong" sort="Wang, Xiurong" uniqKey="Wang X" first="Xiurong" last="Wang">Xiurong Wang</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30288747</idno><idno type="pmid">30288747</idno><idno type="doi">10.1111/ppl.12847</idno><idno type="wicri:Area/Main/Corpus">000723</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000723</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transcriptomic analysis reveals the possible roles of sugar metabolism and export for positive mycorrhizal growth responses in soybean.</title><author><name sortKey="Zhao, Shaopeng" sort="Zhao, Shaopeng" uniqKey="Zhao S" first="Shaopeng" last="Zhao">Shaopeng Zhao</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, A" sort="Chen, A" uniqKey="Chen A" first="A" last="Chen">A. Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Chengjie" sort="Chen, Chengjie" uniqKey="Chen C" first="Chengjie" last="Chen">Chengjie Chen</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Chengchen" sort="Li, Chengchen" uniqKey="Li C" first="Chengchen" last="Li">Chengchen Li</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xia, Rui" sort="Xia, Rui" uniqKey="Xia R" first="Rui" last="Xia">Rui Xia</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Xiurong" sort="Wang, Xiurong" uniqKey="Wang X" first="Xiurong" last="Wang">Xiurong Wang</name><affiliation><nlm:affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Profiling (methods)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Mycorrhizae (physiology)</term><term>Plant Roots (genetics)</term><term>Plant Roots (microbiology)</term><term>Sequence Analysis, RNA (MeSH)</term><term>Soybeans (genetics)</term><term>Soybeans (microbiology)</term><term>Symbiosis (genetics)</term><term>Symbiosis (physiology)</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plant Roots</term><term>Soybeans</term><term>Symbiosis</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Soybeans</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Mycorrhizae</term><term>Symbiosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Sequence Analysis, RNA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To elucidate molecular mechanisms controlling differential growth responses to root colonization by arbuscular mycorrhizal (AM) fungi varying in colonization and cooperative behavior, a pot experiment was carried out using two soybean genotypes and three AM inocula. The results showed that inoculation by cooperative Rhizophagus irregularis (Ri) or less cooperative Glomus aggregatum with high AM colonization (Ga-H) significantly promoted plant growth compared with inoculation by G. aggregatum with low AM colonization (Ga-L). A comparative RNA sequencing analysis of the root transcriptomes showed that fatty acid synthesis pathway was significantly enriched in all three AM inoculation roots. However, sugar metabolism and transport were significantly enriched only in Ri and Ga-H inoculation, which was consistent with positive growth responses in these two inoculation treatments. Accordingly, the expression levels of the key genes related to sugar metabolism and transport were also upregulated in Ri and Ga-H roots compared with Ga-L roots. Of them, two sugars will eventually be exported transporters (SWEET) transporter genes, GmSWEET6 (Glyma.04G198600) and GmSWEET15 (Glyma.06G166800), and one invertase (Glyma.17G227900) gene were exclusively induced only in Ri and Ga-H roots. Promoter analyses in transgenic soybean roots further demonstrated that GUS driven by the GmSWEET6 promoter was highly expressed in arbuscule-containing cortical cells. Additionally, Ri and Ga-H inoculation increased the contents of sucrose, glucose and fructose in both shoots and roots compared with those of Ga-L and non-mycorrhizal. These results imply that positive mycorrhizal growth responses in plants might mostly be due to the stimulation of photosynthate metabolism and transport by AM fungal inoculum with high colonization capabilities.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30288747</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2019</Year><Month>08</Month><Day>06</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1399-3054</ISSN><JournalIssue CitedMedium="Internet"><Volume>166</Volume><Issue>3</Issue><PubDate><Year>2019</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Physiologia plantarum</Title><ISOAbbreviation>Physiol Plant</ISOAbbreviation></Journal><ArticleTitle>Transcriptomic analysis reveals the possible roles of sugar metabolism and export for positive mycorrhizal growth responses in soybean.</ArticleTitle><Pagination><MedlinePgn>712-728</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/ppl.12847</ELocationID><Abstract><AbstractText>To elucidate molecular mechanisms controlling differential growth responses to root colonization by arbuscular mycorrhizal (AM) fungi varying in colonization and cooperative behavior, a pot experiment was carried out using two soybean genotypes and three AM inocula. The results showed that inoculation by cooperative Rhizophagus irregularis (Ri) or less cooperative Glomus aggregatum with high AM colonization (Ga-H) significantly promoted plant growth compared with inoculation by G. aggregatum with low AM colonization (Ga-L). A comparative RNA sequencing analysis of the root transcriptomes showed that fatty acid synthesis pathway was significantly enriched in all three AM inoculation roots. However, sugar metabolism and transport were significantly enriched only in Ri and Ga-H inoculation, which was consistent with positive growth responses in these two inoculation treatments. Accordingly, the expression levels of the key genes related to sugar metabolism and transport were also upregulated in Ri and Ga-H roots compared with Ga-L roots. Of them, two sugars will eventually be exported transporters (SWEET) transporter genes, GmSWEET6 (Glyma.04G198600) and GmSWEET15 (Glyma.06G166800), and one invertase (Glyma.17G227900) gene were exclusively induced only in Ri and Ga-H roots. Promoter analyses in transgenic soybean roots further demonstrated that GUS driven by the GmSWEET6 promoter was highly expressed in arbuscule-containing cortical cells. Additionally, Ri and Ga-H inoculation increased the contents of sucrose, glucose and fructose in both shoots and roots compared with those of Ga-L and non-mycorrhizal. These results imply that positive mycorrhizal growth responses in plants might mostly be due to the stimulation of photosynthate metabolism and transport by AM fungal inoculum with high colonization capabilities.</AbstractText><CopyrightInformation>© 2018 Scandinavian Plant Physiology Society.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Shaopeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Chengjie</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chengchen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Rui</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiurong</ForeName><Initials>X</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-0699-8233</Identifier><AffiliationInfo><Affiliation>State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Root Biology Center, South China Agricultural University, Guangzhou 510642, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>31672237</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>2017YFD0200200/2017YFD0200203</GrantID><Agency>National Key R&D Program of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>Denmark</Country><MedlineTA>Physiol Plant</MedlineTA><NlmUniqueID>1256322</NlmUniqueID><ISSNLinking>0031-9317</ISSNLinking></MedlineJournalInfo><MeshHeadingList><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017423" MajorTopicYN="N">Sequence Analysis, RNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013025" MajorTopicYN="N">Soybeans</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="N">Symbiosis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>09</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>10</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>10</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>10</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30288747</ArticleId><ArticleId IdType="doi">10.1111/ppl.12847</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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