Structure-Specific Regulation of Nutrient Transport and Metabolism in Arbuscular Mycorrhizal Fungi.
Identifieur interne : 000408 ( Main/Corpus ); précédent : 000407; suivant : 000409Structure-Specific Regulation of Nutrient Transport and Metabolism in Arbuscular Mycorrhizal Fungi.
Auteurs : Hiromu Kameoka ; Taro Maeda ; Nao Okuma ; Masayoshi KawaguchiSource :
- Plant & cell physiology [ 1471-9053 ] ; 2019.
English descriptors
- KwdEn :
- Biological Transport (MeSH), Daucus carota (microbiology), Gene Expression Profiling (MeSH), Gene Expression Regulation, Fungal (MeSH), Gene Library (MeSH), Glomeromycota (genetics), Glomeromycota (physiology), Hyphae (MeSH), Mycelium (MeSH), Mycorrhizae (genetics), Mycorrhizae (physiology), Nutrients (metabolism), Plant Roots (microbiology), Sequence Analysis, RNA (MeSH), Soil (chemistry), Spores, Fungal (MeSH), Symbiosis (MeSH), Transcriptome (MeSH).
- MESH :
- chemical , chemistry : Soil.
- genetics : Glomeromycota, Mycorrhizae.
- metabolism : Nutrients.
- microbiology : Daucus carota, Plant Roots.
- physiology : Glomeromycota, Mycorrhizae.
- Biological Transport, Gene Expression Profiling, Gene Expression Regulation, Fungal, Gene Library, Hyphae, Mycelium, Sequence Analysis, RNA, Spores, Fungal, Symbiosis, Transcriptome.
Abstract
Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with most land plants, mainly for the purpose of nutrient exchange. Many studies have revealed the regulation of processes in AMF, such as nutrient absorption from soil, metabolism and exchange with host plants, and the genes involved. However, the spatial regulation of the genes within the structures comprising each developmental stage is not well understood. Here, we demonstrate the structure-specific transcriptome of the model AMF species, Rhizophagus irregularis. We performed an ultra-low input RNA-seq analysis, SMART-seq2, comparing five extraradical structures, germ tubes, runner hyphae, branched absorbing structures (BAS), immature spores and mature spores. In addition, we reanalyzed the recently reported RNA-seq data comparing intraradical mycelium and arbuscule. Our analyses captured the distinct features of each structure and revealed the structure-specific expression patterns of genes related to nutrient transport and metabolism. Of note, the transcriptional profiles suggest distinct functions of BAS in nutrient absorption. These findings provide a comprehensive dataset to advance our understanding of the transcriptional dynamics of fungal nutrition in this symbiotic system.
DOI: 10.1093/pcp/pcz122
PubMed: 31241164
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Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kawaguchi, Masayoshi" sort="Kawaguchi, Masayoshi" uniqKey="Kawaguchi M" first="Masayoshi" last="Kawaguchi">Masayoshi Kawaguchi</name><affiliation><nlm:affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31241164</idno><idno type="pmid">31241164</idno><idno type="doi">10.1093/pcp/pcz122</idno><idno type="wicri:Area/Main/Corpus">000408</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" 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University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation></author><author><name sortKey="Kawaguchi, Masayoshi" sort="Kawaguchi, Masayoshi" uniqKey="Kawaguchi M" first="Masayoshi" last="Kawaguchi">Masayoshi Kawaguchi</name><affiliation><nlm:affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>The Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biological Transport (MeSH)</term><term>Daucus carota (microbiology)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Fungal (MeSH)</term><term>Gene Library (MeSH)</term><term>Glomeromycota (genetics)</term><term>Glomeromycota (physiology)</term><term>Hyphae (MeSH)</term><term>Mycelium (MeSH)</term><term>Mycorrhizae (genetics)</term><term>Mycorrhizae (physiology)</term><term>Nutrients (metabolism)</term><term>Plant Roots (microbiology)</term><term>Sequence Analysis, RNA (MeSH)</term><term>Soil (chemistry)</term><term>Spores, Fungal (MeSH)</term><term>Symbiosis (MeSH)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nutrients</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Daucus carota</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Transport</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Fungal</term><term>Gene Library</term><term>Hyphae</term><term>Mycelium</term><term>Sequence Analysis, RNA</term><term>Spores, Fungal</term><term>Symbiosis</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with most land plants, mainly for the purpose of nutrient exchange. Many studies have revealed the regulation of processes in AMF, such as nutrient absorption from soil, metabolism and exchange with host plants, and the genes involved. However, the spatial regulation of the genes within the structures comprising each developmental stage is not well understood. Here, we demonstrate the structure-specific transcriptome of the model AMF species, Rhizophagus irregularis. We performed an ultra-low input RNA-seq analysis, SMART-seq2, comparing five extraradical structures, germ tubes, runner hyphae, branched absorbing structures (BAS), immature spores and mature spores. In addition, we reanalyzed the recently reported RNA-seq data comparing intraradical mycelium and arbuscule. Our analyses captured the distinct features of each structure and revealed the structure-specific expression patterns of genes related to nutrient transport and metabolism. Of note, the transcriptional profiles suggest distinct functions of BAS in nutrient absorption. These findings provide a comprehensive dataset to advance our understanding of the transcriptional dynamics of fungal nutrition in this symbiotic system.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31241164</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>03</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>11</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1471-9053</ISSN><JournalIssue CitedMedium="Internet"><Volume>60</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>Oct</Month><Day>01</Day></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Structure-Specific Regulation of Nutrient Transport and Metabolism in Arbuscular Mycorrhizal Fungi.</ArticleTitle><Pagination><MedlinePgn>2272-2281</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/pcp/pcz122</ELocationID><Abstract><AbstractText>Arbuscular mycorrhizal fungi (AMF) establish symbiotic relationships with most land plants, mainly for the purpose of nutrient exchange. Many studies have revealed the regulation of processes in AMF, such as nutrient absorption from soil, metabolism and exchange with host plants, and the genes involved. However, the spatial regulation of the genes within the structures comprising each developmental stage is not well understood. Here, we demonstrate the structure-specific transcriptome of the model AMF species, Rhizophagus irregularis. We performed an ultra-low input RNA-seq analysis, SMART-seq2, comparing five extraradical structures, germ tubes, runner hyphae, branched absorbing structures (BAS), immature spores and mature spores. In addition, we reanalyzed the recently reported RNA-seq data comparing intraradical mycelium and arbuscule. Our analyses captured the distinct features of each structure and revealed the structure-specific expression patterns of genes related to nutrient transport and metabolism. Of note, the transcriptional profiles suggest distinct functions of BAS in nutrient absorption. These findings provide a comprehensive dataset to advance our understanding of the transcriptional dynamics of fungal nutrition in this symbiotic system.</AbstractText><CopyrightInformation>� The Author(s) 2019. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kameoka</LastName><ForeName>Hiromu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maeda</LastName><ForeName>Taro</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Division of Symbiotic Systems, National Institute for Basic Biology, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Okuma</LastName><ForeName>Nao</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>The Graduate University for Advanced Studies, 38 Nishigonaka, Myodaiji, Okazaki, Aichi, Japan.</Affiliation></AffiliationInfo></Author><Author 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MajorTopicYN="N">Biological Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018552" MajorTopicYN="N">Daucus carota</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="Y">Gene Expression Regulation, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015723" MajorTopicYN="N">Gene Library</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D025282" 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