Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations.
Identifieur interne : 000343 ( Main/Corpus ); précédent : 000342; suivant : 000344Ca2+-regulated Ca2+ channels with an RCK gating ring control plant symbiotic associations.
Auteurs : Sunghoon Kim ; Weizhong Zeng ; Shane Bernard ; Jun Liao ; Muthusubramanian Venkateshwaran ; Jean-Michel Ane ; Youxing JiangSource :
- Nature communications [ 2041-1723 ] ; 2019.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Calcium, Calcium Channels, Plant Proteins.
- metabolism : Nuclear Envelope.
- Ion Channel Gating, Lotus, Mycorrhizae, Protein Domains, Rhizobium, Symbiosis.
Abstract
A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca2+ spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca2+ channel whose activation requires cytosolic/nucleosolic Ca2+, contrary to the previous suggestion of it being a K+ channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K+) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca2+-gated K+ (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca2+ sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca2+ binding sites to the physiological function of CASTOR as well as DMI1.
DOI: 10.1038/s41467-019-11698-5
PubMed: 31420535
PubMed Central: PMC6697748
Links to Exploration step
pubmed:31420535Le document en format XML
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USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bernard, Shane" sort="Bernard, Shane" uniqKey="Bernard S" first="Shane" last="Bernard">Shane Bernard</name><affiliation><nlm:affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Liao, Jun" sort="Liao, Jun" uniqKey="Liao J" first="Jun" last="Liao">Jun Liao</name><affiliation><nlm:affiliation>School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Venkateshwaran, Muthusubramanian" sort="Venkateshwaran, Muthusubramanian" uniqKey="Venkateshwaran M" first="Muthusubramanian" last="Venkateshwaran">Muthusubramanian Venkateshwaran</name><affiliation><nlm:affiliation>School of Agriculture, University of Wisconsin-Platteville, Platteville, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ane, Jean Michel" sort="Ane, Jean Michel" uniqKey="Ane J" first="Jean-Michel" last="Ane">Jean-Michel Ane</name><affiliation><nlm:affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Youxing" sort="Jiang, Youxing" uniqKey="Jiang Y" first="Youxing" last="Jiang">Youxing Jiang</name><affiliation><nlm:affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31420535</idno><idno type="pmid">31420535</idno><idno type="doi">10.1038/s41467-019-11698-5</idno><idno type="pmc">PMC6697748</idno><idno type="wicri:Area/Main/Corpus">000343</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000343</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Ca<sup>2+</sup>-regulated Ca<sup>2+</sup> channels with an RCK gating ring control plant symbiotic associations.</title><author><name sortKey="Kim, Sunghoon" sort="Kim, Sunghoon" uniqKey="Kim S" first="Sunghoon" last="Kim">Sunghoon Kim</name><affiliation><nlm:affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Weizhong" sort="Zeng, Weizhong" uniqKey="Zeng W" first="Weizhong" last="Zeng">Weizhong Zeng</name><affiliation><nlm:affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Bernard, Shane" sort="Bernard, Shane" uniqKey="Bernard S" first="Shane" last="Bernard">Shane Bernard</name><affiliation><nlm:affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Liao, Jun" sort="Liao, Jun" uniqKey="Liao J" first="Jun" last="Liao">Jun Liao</name><affiliation><nlm:affiliation>School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Venkateshwaran, Muthusubramanian" sort="Venkateshwaran, Muthusubramanian" uniqKey="Venkateshwaran M" first="Muthusubramanian" last="Venkateshwaran">Muthusubramanian Venkateshwaran</name><affiliation><nlm:affiliation>School of Agriculture, University of Wisconsin-Platteville, Platteville, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Ane, Jean Michel" sort="Ane, Jean Michel" uniqKey="Ane J" first="Jean-Michel" last="Ane">Jean-Michel Ane</name><affiliation><nlm:affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Jiang, Youxing" sort="Jiang, Youxing" uniqKey="Jiang Y" first="Youxing" last="Jiang">Youxing Jiang</name><affiliation><nlm:affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Nature communications</title><idno type="eISSN">2041-1723</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Calcium (metabolism)</term><term>Calcium Channels (metabolism)</term><term>Ion Channel Gating (MeSH)</term><term>Lotus (MeSH)</term><term>Mycorrhizae (MeSH)</term><term>Nuclear Envelope (metabolism)</term><term>Plant Proteins (metabolism)</term><term>Protein Domains (MeSH)</term><term>Rhizobium (MeSH)</term><term>Symbiosis (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term><term>Calcium Channels</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nuclear Envelope</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Ion Channel Gating</term><term>Lotus</term><term>Mycorrhizae</term><term>Protein Domains</term><term>Rhizobium</term><term>Symbiosis</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca<sup>2+</sup> spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca<sup>2+</sup> channel whose activation requires cytosolic/nucleosolic Ca<sup>2+</sup>, contrary to the previous suggestion of it being a K<sup>+</sup> channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K<sup>+</sup>) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca<sup>2+</sup>-gated K<sup>+</sup> (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca<sup>2+</sup> sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca<sup>2+</sup> binding sites to the physiological function of CASTOR as well as DMI1.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31420535</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>17</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2041-1723</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>08</Month><Day>16</Day></PubDate></JournalIssue><Title>Nature communications</Title><ISOAbbreviation>Nat Commun</ISOAbbreviation></Journal><ArticleTitle>Ca<sup>2+</sup>-regulated Ca<sup>2+</sup> channels with an RCK gating ring control plant symbiotic associations.</ArticleTitle><Pagination><MedlinePgn>3703</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41467-019-11698-5</ELocationID><Abstract><AbstractText>A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca<sup>2+</sup> spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca<sup>2+</sup> channel whose activation requires cytosolic/nucleosolic Ca<sup>2+</sup>, contrary to the previous suggestion of it being a K<sup>+</sup> channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K<sup>+</sup>) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca<sup>2+</sup>-gated K<sup>+</sup> (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca<sup>2+</sup> sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca<sup>2+</sup> binding sites to the physiological function of CASTOR as well as DMI1.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Sunghoon</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-5351-453X</Identifier><AffiliationInfo><Affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Weizhong</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bernard</LastName><ForeName>Shane</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liao</LastName><ForeName>Jun</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0001-7487-5545</Identifier><AffiliationInfo><Affiliation>School of Life Science and Technology, ShanghaiTech University, 201210, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Venkateshwaran</LastName><ForeName>Muthusubramanian</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>School of Agriculture, University of Wisconsin-Platteville, Platteville, WI, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ane</LastName><ForeName>Jean-Michel</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Youxing</ForeName><Initials>Y</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-1874-0504</Identifier><AffiliationInfo><Affiliation>Howard Hughes Medical Institute and Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, USA. youxing.jiang@utsouthwestern.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>R01 GM079179</GrantID><Acronym>GM</Acronym><Agency>NIGMS NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>08</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nat Commun</MedlineTA><NlmUniqueID>101528555</NlmUniqueID><ISSNLinking>2041-1723</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015220">Calcium Channels</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber><NameOfSubstance UI="D002118">Calcium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>Nat Commun. 2019 Oct 7;10(1):4607</RefSource><PMID Version="1">31591395</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015220" MajorTopicYN="N">Calcium Channels</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015640" MajorTopicYN="N">Ion Channel Gating</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000070116" MajorTopicYN="Y">Lotus</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="Y">Mycorrhizae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009685" MajorTopicYN="N">Nuclear Envelope</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="Y">Rhizobium</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013559" MajorTopicYN="Y">Symbiosis</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>02</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31420535</ArticleId><ArticleId IdType="doi">10.1038/s41467-019-11698-5</ArticleId><ArticleId IdType="pii">10.1038/s41467-019-11698-5</ArticleId><ArticleId IdType="pmc">PMC6697748</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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