Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.
Identifieur interne : 000066 ( Main/Corpus ); précédent : 000065; suivant : 000067Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.
Auteurs : Shuangshuang Wang ; Aiqun Chen ; Kun Xie ; Xiaofeng Yang ; Zhenzhen Luo ; Jiadong Chen ; Dechao Zeng ; Yuhan Ren ; Congfan Yang ; Lingxiao Wang ; Huimin Feng ; Damar Lizbeth L Pez-Arredondo ; Luis Rafael Herrera-Estrella ; Guohua XuSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.
English descriptors
- KwdEn :
- Anion Transport Proteins (genetics), Anion Transport Proteins (metabolism), Gene Expression Regulation, Plant (MeSH), Glomeromycota (physiology), Mycorrhizae (physiology), Nitrates (metabolism), Nitrogen (metabolism), Oryza (genetics), Oryza (growth & development), Oryza (metabolism), Oryza (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (growth & development), Plant Roots (metabolism), Plant Roots (microbiology), Sorghum (genetics), Sorghum (metabolism), Sorghum (microbiology), Zea mays (genetics), Zea mays (metabolism), Zea mays (microbiology).
- MESH :
- chemical , genetics : Anion Transport Proteins, Plant Proteins.
- chemical , metabolism : Anion Transport Proteins, Nitrates, Nitrogen, Plant Proteins.
- genetics : Oryza, Plant Roots, Sorghum, Zea mays.
- growth & development : Oryza, Plant Roots.
- metabolism : Oryza, Plant Roots, Sorghum, Zea mays.
- microbiology : Oryza, Plant Roots, Sorghum, Zea mays.
- physiology : Glomeromycota, Mycorrhizae.
- Gene Expression Regulation, Plant.
Abstract
Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus Rhizophagus irregularis remarkably promoted rice (Oryza sativa) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO3- supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene OsNPF4.5 in rice roots, and its orthologs ZmNPF4.5 in Zea mays and SbNPF4.5 in Sorghum bicolor OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO3- transport activity when expressed in Xenopus laevis oocytes. Moreover, knockout of OsNPF4.5 resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO3- was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO3- acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.
DOI: 10.1073/pnas.2000926117
PubMed: 32586957
PubMed Central: PMC7368293
Links to Exploration step
pubmed:32586957Le document en format XML
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Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Kun" sort="Xie, Kun" uniqKey="Xie K" first="Kun" last="Xie">Kun Xie</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Zhenzhen" sort="Luo, Zhenzhen" uniqKey="Luo Z" first="Zhenzhen" last="Luo">Zhenzhen Luo</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Dechao" sort="Zeng, Dechao" uniqKey="Zeng D" first="Dechao" last="Zeng">Dechao Zeng</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Yuhan" sort="Ren, Yuhan" uniqKey="Ren Y" first="Yuhan" last="Ren">Yuhan Ren</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Congfan" sort="Yang, Congfan" uniqKey="Yang C" first="Congfan" last="Yang">Congfan Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lingxiao" sort="Wang, Lingxiao" uniqKey="Wang L" first="Lingxiao" last="Wang">Lingxiao Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Feng, Huimin" sort="Feng, Huimin" uniqKey="Feng H" first="Huimin" last="Feng">Huimin Feng</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="L Pez Arredondo, Damar Lizbeth" sort="L Pez Arredondo, Damar Lizbeth" uniqKey="L Pez Arredondo D" first="Damar Lizbeth" last="L Pez-Arredondo">Damar Lizbeth L Pez-Arredondo</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation></affiliation></author><author><name sortKey="Herrera Estrella, Luis Rafael" sort="Herrera Estrella, Luis Rafael" uniqKey="Herrera Estrella L" first="Luis Rafael" last="Herrera-Estrella">Luis Rafael Herrera-Estrella</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 36500 Irapuato, Mexico.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32586957</idno><idno type="pmid">32586957</idno><idno type="doi">10.1073/pnas.2000926117</idno><idno type="pmc">PMC7368293</idno><idno type="wicri:Area/Main/Corpus">000066</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000066</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.</title><author><name sortKey="Wang, Shuangshuang" sort="Wang, Shuangshuang" uniqKey="Wang S" first="Shuangshuang" last="Wang">Shuangshuang Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Kun" sort="Xie, Kun" uniqKey="Xie K" first="Kun" last="Xie">Kun Xie</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Zhenzhen" sort="Luo, Zhenzhen" uniqKey="Luo Z" first="Zhenzhen" last="Luo">Zhenzhen Luo</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zeng, Dechao" sort="Zeng, Dechao" uniqKey="Zeng D" first="Dechao" last="Zeng">Dechao Zeng</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ren, Yuhan" sort="Ren, Yuhan" uniqKey="Ren Y" first="Yuhan" last="Ren">Yuhan Ren</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, Congfan" sort="Yang, Congfan" uniqKey="Yang C" first="Congfan" last="Yang">Congfan Yang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Lingxiao" sort="Wang, Lingxiao" uniqKey="Wang L" first="Lingxiao" last="Wang">Lingxiao Wang</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="Feng, Huimin" sort="Feng, Huimin" uniqKey="Feng H" first="Huimin" last="Feng">Huimin Feng</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author><author><name sortKey="L Pez Arredondo, Damar Lizbeth" sort="L Pez Arredondo, Damar Lizbeth" uniqKey="L Pez Arredondo D" first="Damar Lizbeth" last="L Pez-Arredondo">Damar Lizbeth L Pez-Arredondo</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation></affiliation></author><author><name sortKey="Herrera Estrella, Luis Rafael" sort="Herrera Estrella, Luis Rafael" uniqKey="Herrera Estrella L" first="Luis Rafael" last="Herrera-Estrella">Luis Rafael Herrera-Estrella</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 36500 Irapuato, Mexico.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name><affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anion Transport Proteins (genetics)</term><term>Anion Transport Proteins (metabolism)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glomeromycota (physiology)</term><term>Mycorrhizae (physiology)</term><term>Nitrates (metabolism)</term><term>Nitrogen (metabolism)</term><term>Oryza (genetics)</term><term>Oryza (growth & development)</term><term>Oryza (metabolism)</term><term>Oryza (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plant Roots (genetics)</term><term>Plant Roots (growth & development)</term><term>Plant Roots (metabolism)</term><term>Plant Roots (microbiology)</term><term>Sorghum (genetics)</term><term>Sorghum (metabolism)</term><term>Sorghum (microbiology)</term><term>Zea mays (genetics)</term><term>Zea mays (metabolism)</term><term>Zea mays (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Anion Transport Proteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anion Transport Proteins</term><term>Nitrates</term><term>Nitrogen</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term><term>Plant Roots</term><term>Sorghum</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oryza</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term><term>Plant Roots</term><term>Sorghum</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Oryza</term><term>Plant Roots</term><term>Sorghum</term><term>Zea mays</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus <i>Rhizophagus irregularis</i> remarkably promoted rice (<i>Oryza sativa</i>) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO<sub>3</sub><sup>-</sup> supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene <i>OsNPF4.5</i> in rice roots, and its orthologs <i>ZmNPF4.5</i> in <i>Zea mays</i> and <i>SbNPF4.5</i> in <i>Sorghum bicolor</i> OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO<sub>3</sub><sup>-</sup> transport activity when expressed in <i>Xenopus laevis</i> oocytes. Moreover, knockout of <i>OsNPF4.5</i> resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO<sub>3</sub><sup>-</sup> was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO<sub>3</sub><sup>-</sup> acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32586957</PMID><DateCompleted><Year>2020</Year><Month>09</Month><Day>21</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>117</Volume><Issue>28</Issue><PubDate><Year>2020</Year><Month>07</Month><Day>14</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation></Journal><ArticleTitle>Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.</ArticleTitle><Pagination><MedlinePgn>16649-16659</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.2000926117</ELocationID><Abstract><AbstractText>Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus <i>Rhizophagus irregularis</i> remarkably promoted rice (<i>Oryza sativa</i>) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO<sub>3</sub><sup>-</sup> supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene <i>OsNPF4.5</i> in rice roots, and its orthologs <i>ZmNPF4.5</i> in <i>Zea mays</i> and <i>SbNPF4.5</i> in <i>Sorghum bicolor</i> OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO<sub>3</sub><sup>-</sup> transport activity when expressed in <i>Xenopus laevis</i> oocytes. Moreover, knockout of <i>OsNPF4.5</i> resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO<sub>3</sub><sup>-</sup> was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO<sub>3</sub><sup>-</sup> acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.</AbstractText><CopyrightInformation>Copyright © 2020 the Author(s). Published by PNAS.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shuangshuang</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Aiqun</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-1679-4226</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Kun</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiaofeng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Zhenzhen</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jiadong</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Dechao</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Yuhan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Congfan</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Lingxiao</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Huimin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Arredondo</LastName><ForeName>Damar Lizbeth</ForeName><Initials>DL</Initials><Identifier Source="ORCID">0000-0001-7389-3143</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Herrera-Estrella</LastName><ForeName>Luis Rafael</ForeName><Initials>LR</Initials><Identifier Source="ORCID">0000-0001-7936-3856</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 36500 Irapuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Guohua</ForeName><Initials>G</Initials><Identifier Source="ORCID">0000-0002-3283-2392</Identifier><AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D027321">Anion Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009566">Nitrates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C078921">nitrate transporters</NameOfSubstance></Chemical><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D027321" MajorTopicYN="N">Anion Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045868" MajorTopicYN="N">Sorghum</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">OsNPF4.5</Keyword><Keyword MajorTopicYN="Y">RNA sequencing</Keyword><Keyword MajorTopicYN="Y">arbuscular mycorrhiza</Keyword><Keyword MajorTopicYN="Y">nitrate transporter</Keyword><Keyword MajorTopicYN="Y">nitrogen uptake</Keyword></KeywordList><CoiStatement>The authors declare no competing interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>9</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32586957</ArticleId><ArticleId IdType="pii">2000926117</ArticleId><ArticleId IdType="doi">10.1073/pnas.2000926117</ArticleId><ArticleId 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