Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.
Identifieur interne : 000066 ( Main/Corpus ); précédent : 000065; suivant : 000067Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.
Auteurs : Shuangshuang Wang ; Aiqun Chen ; Kun Xie ; Xiaofeng Yang ; Zhenzhen Luo ; Jiadong Chen ; Dechao Zeng ; Yuhan Ren ; Congfan Yang ; Lingxiao Wang ; Huimin Feng ; Damar Lizbeth L Pez-Arredondo ; Luis Rafael Herrera-Estrella ; Guohua XuSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2020.
English descriptors
- KwdEn :
- Anion Transport Proteins (genetics), Anion Transport Proteins (metabolism), Gene Expression Regulation, Plant (MeSH), Glomeromycota (physiology), Mycorrhizae (physiology), Nitrates (metabolism), Nitrogen (metabolism), Oryza (genetics), Oryza (growth & development), Oryza (metabolism), Oryza (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plant Roots (genetics), Plant Roots (growth & development), Plant Roots (metabolism), Plant Roots (microbiology), Sorghum (genetics), Sorghum (metabolism), Sorghum (microbiology), Zea mays (genetics), Zea mays (metabolism), Zea mays (microbiology).
- MESH :
- chemical , genetics : Anion Transport Proteins, Plant Proteins.
- chemical , metabolism : Anion Transport Proteins, Nitrates, Nitrogen, Plant Proteins.
- genetics : Oryza, Plant Roots, Sorghum, Zea mays.
- growth & development : Oryza, Plant Roots.
- metabolism : Oryza, Plant Roots, Sorghum, Zea mays.
- microbiology : Oryza, Plant Roots, Sorghum, Zea mays.
- physiology : Glomeromycota, Mycorrhizae.
- Gene Expression Regulation, Plant.
Abstract
Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus Rhizophagus irregularis remarkably promoted rice (Oryza sativa) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO3- supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene OsNPF4.5 in rice roots, and its orthologs ZmNPF4.5 in Zea mays and SbNPF4.5 in Sorghum bicolor OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO3- transport activity when expressed in Xenopus laevis oocytes. Moreover, knockout of OsNPF4.5 resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO3- was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO3- acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.
DOI: 10.1073/pnas.2000926117
PubMed: 32586957
PubMed Central: PMC7368293
Links to Exploration step
pubmed:32586957Le document en format XML
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<author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name>
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<author><name sortKey="Xie, Kun" sort="Xie, Kun" uniqKey="Xie K" first="Kun" last="Xie">Kun Xie</name>
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<author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name>
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<author><name sortKey="Ren, Yuhan" sort="Ren, Yuhan" uniqKey="Ren Y" first="Yuhan" last="Ren">Yuhan Ren</name>
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<author><name sortKey="Yang, Congfan" sort="Yang, Congfan" uniqKey="Yang C" first="Congfan" last="Yang">Congfan Yang</name>
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<author><name sortKey="Wang, Lingxiao" sort="Wang, Lingxiao" uniqKey="Wang L" first="Lingxiao" last="Wang">Lingxiao Wang</name>
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</affiliation>
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<author><name sortKey="Herrera Estrella, Luis Rafael" sort="Herrera Estrella, Luis Rafael" uniqKey="Herrera Estrella L" first="Luis Rafael" last="Herrera-Estrella">Luis Rafael Herrera-Estrella</name>
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<author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation>
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<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.</title>
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</affiliation>
<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
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<author><name sortKey="Chen, Aiqun" sort="Chen, Aiqun" uniqKey="Chen A" first="Aiqun" last="Chen">Aiqun Chen</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation>
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<author><name sortKey="Xie, Kun" sort="Xie, Kun" uniqKey="Xie K" first="Kun" last="Xie">Kun Xie</name>
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</affiliation>
<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Yang, Xiaofeng" sort="Yang, Xiaofeng" uniqKey="Yang X" first="Xiaofeng" last="Yang">Xiaofeng Yang</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
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<author><name sortKey="Luo, Zhenzhen" sort="Luo, Zhenzhen" uniqKey="Luo Z" first="Zhenzhen" last="Luo">Zhenzhen Luo</name>
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</affiliation>
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<author><name sortKey="Chen, Jiadong" sort="Chen, Jiadong" uniqKey="Chen J" first="Jiadong" last="Chen">Jiadong Chen</name>
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</affiliation>
<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
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<author><name sortKey="Zeng, Dechao" sort="Zeng, Dechao" uniqKey="Zeng D" first="Dechao" last="Zeng">Dechao Zeng</name>
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</affiliation>
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<author><name sortKey="Ren, Yuhan" sort="Ren, Yuhan" uniqKey="Ren Y" first="Yuhan" last="Ren">Yuhan Ren</name>
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</affiliation>
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<author><name sortKey="Yang, Congfan" sort="Yang, Congfan" uniqKey="Yang C" first="Congfan" last="Yang">Congfan Yang</name>
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</affiliation>
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<author><name sortKey="Wang, Lingxiao" sort="Wang, Lingxiao" uniqKey="Wang L" first="Lingxiao" last="Wang">Lingxiao Wang</name>
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</affiliation>
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<author><name sortKey="Feng, Huimin" sort="Feng, Huimin" uniqKey="Feng H" first="Huimin" last="Feng">Huimin Feng</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="L Pez Arredondo, Damar Lizbeth" sort="L Pez Arredondo, Damar Lizbeth" uniqKey="L Pez Arredondo D" first="Damar Lizbeth" last="L Pez-Arredondo">Damar Lizbeth L Pez-Arredondo</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Herrera Estrella, Luis Rafael" sort="Herrera Estrella, Luis Rafael" uniqKey="Herrera Estrella L" first="Luis Rafael" last="Herrera-Estrella">Luis Rafael Herrera-Estrella</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</nlm:affiliation>
</affiliation>
<affiliation><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 36500 Irapuato, Mexico.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Xu, Guohua" sort="Xu, Guohua" uniqKey="Xu G" first="Guohua" last="Xu">Guohua Xu</name>
<affiliation><nlm:affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</nlm:affiliation>
</affiliation>
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</affiliation>
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<term>Anion Transport Proteins (metabolism)</term>
<term>Gene Expression Regulation, Plant (MeSH)</term>
<term>Glomeromycota (physiology)</term>
<term>Mycorrhizae (physiology)</term>
<term>Nitrates (metabolism)</term>
<term>Nitrogen (metabolism)</term>
<term>Oryza (genetics)</term>
<term>Oryza (growth & development)</term>
<term>Oryza (metabolism)</term>
<term>Oryza (microbiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plant Roots (genetics)</term>
<term>Plant Roots (growth & development)</term>
<term>Plant Roots (metabolism)</term>
<term>Plant Roots (microbiology)</term>
<term>Sorghum (genetics)</term>
<term>Sorghum (metabolism)</term>
<term>Sorghum (microbiology)</term>
<term>Zea mays (genetics)</term>
<term>Zea mays (metabolism)</term>
<term>Zea mays (microbiology)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Anion Transport Proteins</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anion Transport Proteins</term>
<term>Nitrates</term>
<term>Nitrogen</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Oryza</term>
<term>Plant Roots</term>
<term>Sorghum</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oryza</term>
<term>Plant Roots</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Oryza</term>
<term>Plant Roots</term>
<term>Sorghum</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Oryza</term>
<term>Plant Roots</term>
<term>Sorghum</term>
<term>Zea mays</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Glomeromycota</term>
<term>Mycorrhizae</term>
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<front><div type="abstract" xml:lang="en">Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus <i>Rhizophagus irregularis</i>
remarkably promoted rice (<i>Oryza sativa</i>
) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO<sub>3</sub>
<sup>-</sup>
supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene <i>OsNPF4.5</i>
in rice roots, and its orthologs <i>ZmNPF4.5</i>
in <i>Zea mays</i>
and <i>SbNPF4.5</i>
in <i>Sorghum bicolor</i>
OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO<sub>3</sub>
<sup>-</sup>
transport activity when expressed in <i>Xenopus laevis</i>
oocytes. Moreover, knockout of <i>OsNPF4.5</i>
resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO<sub>3</sub>
<sup>-</sup>
was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO<sub>3</sub>
<sup>-</sup>
acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32586957</PMID>
<DateCompleted><Year>2020</Year>
<Month>09</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>09</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN>
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<Issue>28</Issue>
<PubDate><Year>2020</Year>
<Month>07</Month>
<Day>14</Day>
</PubDate>
</JournalIssue>
<Title>Proceedings of the National Academy of Sciences of the United States of America</Title>
<ISOAbbreviation>Proc Natl Acad Sci U S A</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional analysis of the OsNPF4.5 nitrate transporter reveals a conserved mycorrhizal pathway of nitrogen acquisition in plants.</ArticleTitle>
<Pagination><MedlinePgn>16649-16659</MedlinePgn>
</Pagination>
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<Abstract><AbstractText>Low availability of nitrogen (N) is often a major limiting factor to crop yield in most nutrient-poor soils. Arbuscular mycorrhizal (AM) fungi are beneficial symbionts of most land plants that enhance plant nutrient uptake, particularly of phosphate. A growing number of reports point to the substantially increased N accumulation in many mycorrhizal plants; however, the contribution of AM symbiosis to plant N nutrition and the mechanisms underlying the AM-mediated N acquisition are still in the early stages of being understood. Here, we report that inoculation with AM fungus <i>Rhizophagus irregularis</i>
remarkably promoted rice (<i>Oryza sativa</i>
) growth and N acquisition, and about 42% of the overall N acquired by rice roots could be delivered via the symbiotic route under N-NO<sub>3</sub>
<sup>-</sup>
supply condition. Mycorrhizal colonization strongly induced expression of the putative nitrate transporter gene <i>OsNPF4.5</i>
in rice roots, and its orthologs <i>ZmNPF4.5</i>
in <i>Zea mays</i>
and <i>SbNPF4.5</i>
in <i>Sorghum bicolor</i>
OsNPF4.5 is exclusively expressed in the cells containing arbuscules and displayed a low-affinity NO<sub>3</sub>
<sup>-</sup>
transport activity when expressed in <i>Xenopus laevis</i>
oocytes. Moreover, knockout of <i>OsNPF4.5</i>
resulted in a 45% decrease in symbiotic N uptake and a significant reduction in arbuscule incidence when NO<sub>3</sub>
<sup>-</sup>
was supplied as an N source. Based on our results, we propose that the NPF4.5 plays a key role in mycorrhizal NO<sub>3</sub>
<sup>-</sup>
acquisition, a symbiotic N uptake route that might be highly conserved in gramineous species.</AbstractText>
<CopyrightInformation>Copyright © 2020 the Author(s). Published by PNAS.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Shuangshuang</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Aiqun</ForeName>
<Initials>A</Initials>
<Identifier Source="ORCID">0000-0002-1679-4226</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xie</LastName>
<ForeName>Kun</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Xiaofeng</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Luo</LastName>
<ForeName>Zhenzhen</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Jiadong</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zeng</LastName>
<ForeName>Dechao</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ren</LastName>
<ForeName>Yuhan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Congfan</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Lingxiao</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Feng</LastName>
<ForeName>Huimin</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>López-Arredondo</LastName>
<ForeName>Damar Lizbeth</ForeName>
<Initials>DL</Initials>
<Identifier Source="ORCID">0000-0001-7389-3143</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Herrera-Estrella</LastName>
<ForeName>Luis Rafael</ForeName>
<Initials>LR</Initials>
<Identifier Source="ORCID">0000-0001-7936-3856</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Sciences, Texas Tech University, Lubbock, TX 79409.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Unidad de Genómica Avanzada del Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, 36500 Irapuato, Mexico.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Guohua</ForeName>
<Initials>G</Initials>
<Identifier Source="ORCID">0000-0002-3283-2392</Identifier>
<AffiliationInfo><Affiliation>State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Resources and Environmental Sciences, Nanjing Agricultural University, 210095 Nanjing, China; chenaq8@njau.edu.cn ghxu@njau.edu.cn luis.herrera-estrella@ttu.edu.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095 Nanjing, China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>06</Month>
<Day>25</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Proc Natl Acad Sci U S A</MedlineTA>
<NlmUniqueID>7505876</NlmUniqueID>
<ISSNLinking>0027-8424</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D027321">Anion Transport Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009566">Nitrates</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C078921">nitrate transporters</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>N762921K75</RegistryNumber>
<NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D027321" MajorTopicYN="N">Anion Transport Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D055137" MajorTopicYN="N">Glomeromycota</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009566" MajorTopicYN="N">Nitrates</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045868" MajorTopicYN="N">Sorghum</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003313" MajorTopicYN="N">Zea mays</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">OsNPF4.5</Keyword>
<Keyword MajorTopicYN="Y">RNA sequencing</Keyword>
<Keyword MajorTopicYN="Y">arbuscular mycorrhiza</Keyword>
<Keyword MajorTopicYN="Y">nitrate transporter</Keyword>
<Keyword MajorTopicYN="Y">nitrogen uptake</Keyword>
</KeywordList>
<CoiStatement>The authors declare no competing interest.</CoiStatement>
</MedlineCitation>
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<Month>9</Month>
<Day>22</Day>
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<Day>27</Day>
<Hour>6</Hour>
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<ArticleIdList><ArticleId IdType="pubmed">32586957</ArticleId>
<ArticleId IdType="pii">2000926117</ArticleId>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Plant Cell Environ. 2019 May;42(5):1758-1774</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30578745</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2009 May;150(1):73-83</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19329566</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycorrhiza. 2011 Nov;21(8):659-667</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21448812</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2015 Apr;27(4):1352-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25841038</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1720-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17242358</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2002 Oct 1;99(20):13324-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12271140</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Biotechnol J. 2016 Aug;14(8):1705-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26826052</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2013 May;198(3):853-65</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23461653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2012 Oct;24(10):4236-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23073651</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7118-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27274069</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24753421</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2019 May;180(1):465-479</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30760639</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2017 Jun 16;356(6343):1172-1175</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28596307</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2001 Sep 20;413(6853):297-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11565029</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2014 Apr 29;26(4):1808-1817</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24781114</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2005 Jun 9;435(7043):819-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15944705</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2018 Mar;30(3):638-651</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29475937</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):E9239-E9246</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30209216</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 1996 Jun;133(2):273-280</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29681069</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2011;62:227-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21391813</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Commun Biol. 2019 Jun 21;2:233</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31263777</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2017 Jun 16;356(6343):1175-1178</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28596311</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1999 May;11(5):865-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10330471</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2014 Mar 6;507(7490):73-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24572362</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Plant Biol. 2009 Jan 22;9:10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19161626</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2007;173(4):817-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17286830</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2013 Oct;23(10):1233-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23999856</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 May 31;102(22):8066-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15905328</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2012 Dec;160(4):2052-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23093362</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2017 Jun;214(4):1631-1645</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28380681</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Cell Dev Biol. 2018 Feb;74:80-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28647533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycorrhiza. 2018 Jan;28(1):93-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28993893</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol Biochem. 2014 Feb;75:1-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24361504</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2013 Dec 10;110(50):20117-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24277808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Physiol. 2010 Jul;153(3):1175-87</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20448102</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Genet. 2015 Jul;47(7):834-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26053497</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1999 Aug;11(8):1381-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10449574</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Elife. 2017 Jul 20;6:</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28726631</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Cell Dev Biol. 2013;29:593-617</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24099088</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2018 Jan 8;8(1):4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29311719</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2018 Nov 5;11(11):1344-1359</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30292683</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2011 Mar;189(4):1157-69</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21106037</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2009;182(1):200-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19192192</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2011 Aug 12;333(6044):880-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21836016</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Bot. 2015 Jan;66(1):317-31</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25332358</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>New Phytol. 2009 Mar;181(4):950-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19140941</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W590-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15215457</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant. 2017 Sep 12;10(9):1147-1158</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28782719</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2010 Sep;51(9):1411-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20627949</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2019 Feb 21;10:197</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30846998</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Structure. 2013 Oct 8;21(10):1735-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24035711</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Ann Bot. 2005 Sep;96(4):639-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16024557</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2014 Apr 29;26(4):1818-1830</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24781115</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Plant Biol. 2012;63:153-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22224450</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell Physiol. 2017 Jun 1;58(6):1003-1017</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28387868</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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