Molecular characterization of OsPAP2: transgenic expression of a purple acid phosphatase up-regulated in phosphate-deprived rice suspension cells.
Identifieur interne : 000230 ( Main/Exploration ); précédent : 000229; suivant : 000231Molecular characterization of OsPAP2: transgenic expression of a purple acid phosphatase up-regulated in phosphate-deprived rice suspension cells.
Auteurs : Yeon Jae Hur [Corée du Sud] ; Byung Rae Jin ; Jaesung Nam ; Young Soo Chung ; Jai Heon Lee ; Hong Kyu Choi ; Dae Jin Yun ; Gihwan Yi ; Yong Hwan Kim ; Doh Hoon KimSource :
- Biotechnology letters [ 1573-6776 ] ; 2010.
Descripteurs français
- KwdFr :
- Acid phosphatase (composition chimique), Acid phosphatase (génétique), Acid phosphatase (métabolisme), Animaux (MeSH), Arabidopsis (enzymologie), Arabidopsis (génétique), Arabidopsis (métabolisme), Cellules cultivées (MeSH), Données de séquences moléculaires (MeSH), Glycoprotéines (composition chimique), Glycoprotéines (génétique), Glycoprotéines (métabolisme), Lignée cellulaire (MeSH), Oryza (enzymologie), Oryza (génétique), Phosphates (déficit), Phosphates (physiologie), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Rhizobium (génétique), Réaction de polymérisation en chaîne (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Similitude de séquences d'acides aminés (MeSH), Spodoptera (MeSH), Séquence d'acides aminés (MeSH), Technique de Northern (MeSH), Végétaux génétiquement modifiés (enzymologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- composition chimique : Acid phosphatase, Glycoprotéines, Protéines végétales.
- déficit : Phosphates.
- enzymologie : Arabidopsis, Oryza, Végétaux génétiquement modifiés.
- génétique : Acid phosphatase, Arabidopsis, Glycoprotéines, Oryza, Protéines végétales, Rhizobium, Végétaux génétiquement modifiés.
- métabolisme : Acid phosphatase, Arabidopsis, Glycoprotéines, Protéines végétales, Végétaux génétiquement modifiés.
- physiologie : Phosphates.
- Animaux, Cellules cultivées, Données de séquences moléculaires, Lignée cellulaire, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes végétaux, Similitude de séquences d'acides aminés, Spodoptera, Séquence d'acides aminés, Technique de Northern.
English descriptors
- KwdEn :
- Acid Phosphatase (chemistry), Acid Phosphatase (genetics), Acid Phosphatase (metabolism), Amino Acid Sequence (MeSH), Animals (MeSH), Arabidopsis (enzymology), Arabidopsis (genetics), Arabidopsis (metabolism), Blotting, Northern (MeSH), Cell Line (MeSH), Cells, Cultured (MeSH), Gene Expression Regulation, Plant (MeSH), Glycoproteins (chemistry), Glycoproteins (genetics), Glycoproteins (metabolism), Molecular Sequence Data (MeSH), Oryza (enzymology), Oryza (genetics), Phosphates (deficiency), Phosphates (physiology), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (enzymology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Polymerase Chain Reaction (MeSH), Rhizobium (genetics), Sequence Homology, Amino Acid (MeSH), Spodoptera (MeSH).
- MESH :
- chemical , chemistry : Acid Phosphatase, Glycoproteins, Plant Proteins.
- chemical , deficiency : Phosphates.
- chemical , genetics : Acid Phosphatase, Glycoproteins, Plant Proteins.
- chemical , metabolism : Acid Phosphatase, Glycoproteins, Plant Proteins.
- enzymology : Arabidopsis, Oryza, Plants, Genetically Modified.
- genetics : Arabidopsis, Oryza, Plants, Genetically Modified, Rhizobium.
- metabolism : Arabidopsis, Plants, Genetically Modified.
- chemical , physiology : Phosphates.
- Amino Acid Sequence, Animals, Blotting, Northern, Cell Line, Cells, Cultured, Gene Expression Regulation, Plant, Molecular Sequence Data, Polymerase Chain Reaction, Sequence Homology, Amino Acid, Spodoptera.
Abstract
A phosphate starvation-induced, purple, acid phosphatase cDNA was cloned from rice, Oryza sativa. The cDNA encoding the phosphatase (OsPAP2) has 1,893 bp with an open reading frame of 630 amino acid residues. The deduced amino acid sequence of OsPAP2 shows identities of 60-63% with other plant purple acid phosphatases and appears to have five conserved motifs containing the residues involved in metal binding. OsPAP2 expression is up-regulated in the rice plant and in cell cultures in the absence of phosphate (P( i )). The induced expression of OsPAP2 is a specific response to P( i ) starvation, and is not affected by the deprivation of other nutrients. OsPAP2 expression was responsive to the level of P( i )-supply, and transcripts of OsPAP2 were abundant in P( i )-deprived roots. The OsPAP2 cDNA was expressed as a 69 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsPAP2 gene was introduced into Arabidopsis via an Agrobacterium-mediated transformation. Functional expression of the OsPAP2 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analyses, as well as by phosphatase activity assays. These results suggest that the OsPAP2 gene can be used to develop new transgenic dicotyledonous plants that are able to adapt to P( i )-deficient conditions.
DOI: 10.1007/s10529-009-0131-1
PubMed: 19838636
Affiliations:
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xml:lang="fr">Corée du Sud</country><wicri:regionArea>College of Natural Resources and Life Science, Dong-A University, Busan, 604-714</wicri:regionArea><wicri:noRegion>604-714</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Byung Rae" sort="Jin, Byung Rae" uniqKey="Jin B" first="Byung Rae" last="Jin">Byung Rae Jin</name></author><author><name sortKey="Nam, Jaesung" sort="Nam, Jaesung" uniqKey="Nam J" first="Jaesung" last="Nam">Jaesung Nam</name></author><author><name sortKey="Chung, Young Soo" sort="Chung, Young Soo" uniqKey="Chung Y" first="Young Soo" last="Chung">Young Soo Chung</name></author><author><name sortKey="Lee, Jai Heon" sort="Lee, Jai Heon" uniqKey="Lee J" first="Jai Heon" last="Lee">Jai Heon Lee</name></author><author><name sortKey="Choi, Hong Kyu" sort="Choi, Hong Kyu" uniqKey="Choi H" first="Hong Kyu" last="Choi">Hong Kyu Choi</name></author><author><name sortKey="Yun, Dae Jin" sort="Yun, Dae Jin" uniqKey="Yun D" first="Dae Jin" last="Yun">Dae Jin Yun</name></author><author><name sortKey="Yi, Gihwan" sort="Yi, Gihwan" uniqKey="Yi G" first="Gihwan" last="Yi">Gihwan Yi</name></author><author><name sortKey="Kim, Yong Hwan" sort="Kim, Yong Hwan" uniqKey="Kim Y" first="Yong Hwan" last="Kim">Yong Hwan Kim</name></author><author><name sortKey="Kim, Doh Hoon" sort="Kim, Doh Hoon" uniqKey="Kim D" first="Doh Hoon" last="Kim">Doh Hoon Kim</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acid Phosphatase (chemistry)</term><term>Acid Phosphatase (genetics)</term><term>Acid Phosphatase (metabolism)</term><term>Amino Acid Sequence (MeSH)</term><term>Animals (MeSH)</term><term>Arabidopsis (enzymology)</term><term>Arabidopsis (genetics)</term><term>Arabidopsis (metabolism)</term><term>Blotting, Northern (MeSH)</term><term>Cell Line (MeSH)</term><term>Cells, Cultured (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glycoproteins (chemistry)</term><term>Glycoproteins (genetics)</term><term>Glycoproteins (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Oryza (enzymology)</term><term>Oryza (genetics)</term><term>Phosphates (deficiency)</term><term>Phosphates (physiology)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (enzymology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Rhizobium (genetics)</term><term>Sequence Homology, Amino Acid (MeSH)</term><term>Spodoptera (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acid phosphatase (composition chimique)</term><term>Acid phosphatase (génétique)</term><term>Acid phosphatase (métabolisme)</term><term>Animaux (MeSH)</term><term>Arabidopsis (enzymologie)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Cellules cultivées (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Glycoprotéines (composition chimique)</term><term>Glycoprotéines (génétique)</term><term>Glycoprotéines (métabolisme)</term><term>Lignée cellulaire (MeSH)</term><term>Oryza (enzymologie)</term><term>Oryza (génétique)</term><term>Phosphates (déficit)</term><term>Phosphates (physiologie)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Rhizobium (génétique)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Similitude de séquences d'acides aminés (MeSH)</term><term>Spodoptera (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Technique de Northern (MeSH)</term><term>Végétaux génétiquement modifiés (enzymologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acid Phosphatase</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Phosphates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acid Phosphatase</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acid Phosphatase</term><term>Glycoproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acid phosphatase</term><term>Glycoprotéines</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="déficit" xml:lang="fr"><term>Phosphates</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term><term>Oryza</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term><term>Oryza</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Oryza</term><term>Plants, Genetically Modified</term><term>Rhizobium</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Acid phosphatase</term><term>Arabidopsis</term><term>Glycoprotéines</term><term>Oryza</term><term>Protéines végétales</term><term>Rhizobium</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acid phosphatase</term><term>Arabidopsis</term><term>Glycoprotéines</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Phosphates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Phosphates</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Blotting, Northern</term><term>Cell Line</term><term>Cells, Cultured</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Sequence Homology, Amino Acid</term><term>Spodoptera</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Lignée cellulaire</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes végétaux</term><term>Similitude de séquences d'acides aminés</term><term>Spodoptera</term><term>Séquence d'acides aminés</term><term>Technique de Northern</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A phosphate starvation-induced, purple, acid phosphatase cDNA was cloned from rice, Oryza sativa. The cDNA encoding the phosphatase (OsPAP2) has 1,893 bp with an open reading frame of 630 amino acid residues. The deduced amino acid sequence of OsPAP2 shows identities of 60-63% with other plant purple acid phosphatases and appears to have five conserved motifs containing the residues involved in metal binding. OsPAP2 expression is up-regulated in the rice plant and in cell cultures in the absence of phosphate (P( i )). The induced expression of OsPAP2 is a specific response to P( i ) starvation, and is not affected by the deprivation of other nutrients. OsPAP2 expression was responsive to the level of P( i )-supply, and transcripts of OsPAP2 were abundant in P( i )-deprived roots. The OsPAP2 cDNA was expressed as a 69 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsPAP2 gene was introduced into Arabidopsis via an Agrobacterium-mediated transformation. Functional expression of the OsPAP2 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analyses, as well as by phosphatase activity assays. These results suggest that the OsPAP2 gene can be used to develop new transgenic dicotyledonous plants that are able to adapt to P( i )-deficient conditions.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19838636</PMID><DateCompleted><Year>2010</Year><Month>03</Month><Day>04</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>1</Issue><PubDate><Year>2010</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol Lett</ISOAbbreviation></Journal><ArticleTitle>Molecular characterization of OsPAP2: transgenic expression of a purple acid phosphatase up-regulated in phosphate-deprived rice suspension cells.</ArticleTitle><Pagination><MedlinePgn>163-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-009-0131-1</ELocationID><Abstract><AbstractText>A phosphate starvation-induced, purple, acid phosphatase cDNA was cloned from rice, Oryza sativa. The cDNA encoding the phosphatase (OsPAP2) has 1,893 bp with an open reading frame of 630 amino acid residues. The deduced amino acid sequence of OsPAP2 shows identities of 60-63% with other plant purple acid phosphatases and appears to have five conserved motifs containing the residues involved in metal binding. OsPAP2 expression is up-regulated in the rice plant and in cell cultures in the absence of phosphate (P( i )). The induced expression of OsPAP2 is a specific response to P( i ) starvation, and is not affected by the deprivation of other nutrients. OsPAP2 expression was responsive to the level of P( i )-supply, and transcripts of OsPAP2 were abundant in P( i )-deprived roots. The OsPAP2 cDNA was expressed as a 69 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsPAP2 gene was introduced into Arabidopsis via an Agrobacterium-mediated transformation. Functional expression of the OsPAP2 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analyses, as well as by phosphatase activity assays. These results suggest that the OsPAP2 gene can be used to develop new transgenic dicotyledonous plants that are able to adapt to P( i )-deficient conditions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hur</LastName><ForeName>Yeon Jae</ForeName><Initials>YJ</Initials><AffiliationInfo><Affiliation>College of Natural Resources and Life Science, Dong-A University, Busan, 604-714, Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Byung Rae</ForeName><Initials>BR</Initials></Author><Author ValidYN="Y"><LastName>Nam</LastName><ForeName>Jaesung</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Chung</LastName><ForeName>Young Soo</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Jai Heon</ForeName><Initials>JH</Initials></Author><Author ValidYN="Y"><LastName>Choi</LastName><ForeName>Hong Kyu</ForeName><Initials>HK</Initials></Author><Author ValidYN="Y"><LastName>Yun</LastName><ForeName>Dae Jin</ForeName><Initials>DJ</Initials></Author><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Gihwan</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Yong Hwan</ForeName><Initials>YH</Initials></Author><Author ValidYN="Y"><LastName>Kim</LastName><ForeName>Doh Hoon</ForeName><Initials>DH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>10</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006023">Glycoproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010710">Phosphates</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.3.-</RegistryNumber><NameOfSubstance UI="C091137">purple acid phosphatase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.3.2</RegistryNumber><NameOfSubstance UI="D000135">Acid Phosphatase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000135" MajorTopicYN="N">Acid Phosphatase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015152" MajorTopicYN="N">Blotting, Northern</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006023" MajorTopicYN="N">Glycoproteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012275" MajorTopicYN="N">Oryza</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010710" MajorTopicYN="N">Phosphates</DescriptorName><QualifierName UI="Q000172" MajorTopicYN="Y">deficiency</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017386" MajorTopicYN="N">Sequence Homology, Amino Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018411" MajorTopicYN="N">Spodoptera</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>08</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>09</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>3</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19838636</ArticleId><ArticleId IdType="doi">10.1007/s10529-009-0131-1</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country></list><tree><noCountry><name sortKey="Choi, Hong Kyu" sort="Choi, Hong Kyu" uniqKey="Choi H" first="Hong Kyu" last="Choi">Hong Kyu Choi</name><name sortKey="Chung, Young Soo" sort="Chung, Young Soo" uniqKey="Chung Y" first="Young Soo" last="Chung">Young Soo Chung</name><name sortKey="Jin, Byung Rae" sort="Jin, Byung Rae" uniqKey="Jin B" first="Byung Rae" last="Jin">Byung Rae Jin</name><name sortKey="Kim, Doh Hoon" sort="Kim, Doh Hoon" uniqKey="Kim D" first="Doh Hoon" last="Kim">Doh Hoon Kim</name><name sortKey="Kim, Yong Hwan" sort="Kim, Yong Hwan" uniqKey="Kim Y" first="Yong Hwan" last="Kim">Yong Hwan Kim</name><name sortKey="Lee, Jai Heon" sort="Lee, Jai Heon" uniqKey="Lee J" first="Jai Heon" last="Lee">Jai Heon Lee</name><name sortKey="Nam, Jaesung" sort="Nam, Jaesung" uniqKey="Nam J" first="Jaesung" last="Nam">Jaesung Nam</name><name sortKey="Yi, Gihwan" sort="Yi, Gihwan" uniqKey="Yi G" first="Gihwan" last="Yi">Gihwan Yi</name><name sortKey="Yun, Dae Jin" sort="Yun, Dae Jin" uniqKey="Yun D" first="Dae Jin" last="Yun">Dae Jin Yun</name></noCountry><country name="Corée du Sud"><noRegion><name sortKey="Hur, Yeon Jae" sort="Hur, Yeon Jae" uniqKey="Hur Y" first="Yeon Jae" last="Hur">Yeon Jae Hur</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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