Irreversible, oriented immobilization of antibodies to cobalt-iminodiacetate resin for use as immunoaffinity media.
Identifieur interne : 000485 ( Main/Exploration ); précédent : 000484; suivant : 000486Irreversible, oriented immobilization of antibodies to cobalt-iminodiacetate resin for use as immunoaffinity media.
Auteurs : J E Hale [États-Unis]Source :
- Analytical biochemistry [ 0003-2697 ] ; 1995.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Antibodies, Cobalt, Imino Acids, Immunosorbents, Resins, Plant.
- methods : Chromatography, Affinity.
- Animals, Antibody Affinity, Humans.
Abstract
A method for the construction of antibody affinity columns has been developed which utilizes the innate affinity of antibodies for metal. Antibodies that are reversibly bound to a cobalt-iminodiacetate resin are attached in an "exchange inert" fashion when the cobalt is oxidized from the 2+ to 3+ state. Antibodies bound in this fashion are not removed by metal chelating reagents, high salt, detergents, or chaotropic agents. Only reagents which reduce the metal are capable of removing the antibody. Since the metal binding site in antibodies is in the C-terminus of the heavy chain, antibodies bound to the resin are oriented with the combining site directed away from the resin thus allowing maximal antigen binding. The oxidation conditions used are mild and do not subject the antibody to harsh immobilization chemistry. This technique may be used to construct affinity media using multiple subclasses of antibodies since the metal binding site is conserved. This immobilization strategy may be a general technique for the oriented immobilization of antibody to surfaces.
DOI: 10.1006/abio.1995.1501
PubMed: 8678319
Affiliations:
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Antibodies that are reversibly bound to a cobalt-iminodiacetate resin are attached in an "exchange inert" fashion when the cobalt is oxidized from the 2+ to 3+ state. Antibodies bound in this fashion are not removed by metal chelating reagents, high salt, detergents, or chaotropic agents. Only reagents which reduce the metal are capable of removing the antibody. Since the metal binding site in antibodies is in the C-terminus of the heavy chain, antibodies bound to the resin are oriented with the combining site directed away from the resin thus allowing maximal antigen binding. The oxidation conditions used are mild and do not subject the antibody to harsh immobilization chemistry. This technique may be used to construct affinity media using multiple subclasses of antibodies since the metal binding site is conserved. This immobilization strategy may be a general technique for the oriented immobilization of antibody to surfaces.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8678319</PMID><DateCompleted><Year>1996</Year><Month>08</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0003-2697</ISSN><JournalIssue CitedMedium="Print"><Volume>231</Volume><Issue>1</Issue><PubDate><Year>1995</Year><Month>Oct</Month><Day>10</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal Biochem</ISOAbbreviation></Journal><ArticleTitle>Irreversible, oriented immobilization of antibodies to cobalt-iminodiacetate resin for use as immunoaffinity media.</ArticleTitle><Pagination><MedlinePgn>46-9</MedlinePgn></Pagination><Abstract><AbstractText>A method for the construction of antibody affinity columns has been developed which utilizes the innate affinity of antibodies for metal. Antibodies that are reversibly bound to a cobalt-iminodiacetate resin are attached in an "exchange inert" fashion when the cobalt is oxidized from the 2+ to 3+ state. Antibodies bound in this fashion are not removed by metal chelating reagents, high salt, detergents, or chaotropic agents. Only reagents which reduce the metal are capable of removing the antibody. Since the metal binding site in antibodies is in the C-terminus of the heavy chain, antibodies bound to the resin are oriented with the combining site directed away from the resin thus allowing maximal antigen binding. The oxidation conditions used are mild and do not subject the antibody to harsh immobilization chemistry. This technique may be used to construct affinity media using multiple subclasses of antibodies since the metal binding site is conserved. This immobilization strategy may be a general technique for the oriented immobilization of antibody to surfaces.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hale</LastName><ForeName>J E</ForeName><Initials>JE</Initials><AffiliationInfo><Affiliation>Biotechnology Research Department, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000906">Antibodies</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007098">Imino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007164">Immunosorbents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012116">Resins, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>13869-30-2</RegistryNumber><NameOfSubstance UI="C097998">cobalt iminodiacetate</NameOfSubstance></Chemical><Chemical><RegistryNumber>3G0H8C9362</RegistryNumber><NameOfSubstance UI="D003035">Cobalt</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000906" MajorTopicYN="N">Antibodies</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000915" MajorTopicYN="N">Antibody Affinity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002846" MajorTopicYN="N">Chromatography, Affinity</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003035" MajorTopicYN="N">Cobalt</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007098" MajorTopicYN="N">Imino Acids</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007164" MajorTopicYN="Y">Immunosorbents</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012116" MajorTopicYN="N">Resins, Plant</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1995</Year><Month>10</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1995</Year><Month>10</Month><Day>10</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1995</Year><Month>10</Month><Day>10</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">8678319</ArticleId><ArticleId IdType="pii">S0003-2697(85)71501-1</ArticleId><ArticleId IdType="doi">10.1006/abio.1995.1501</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Hale, J E" sort="Hale, J E" uniqKey="Hale J" first="J E" last="Hale">J E Hale</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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