Detoxification of arsenic by phytochelatins in plants.
Identifieur interne : 000440 ( Main/Corpus ); précédent : 000439; suivant : 000441Detoxification of arsenic by phytochelatins in plants.
Auteurs : M E Schmöger ; M. Oven ; E. GrillSource :
- Plant physiology [ 0032-0889 ] ; 2000.
English descriptors
- KwdEn :
- Arsenic (metabolism), Arsenic (toxicity), Binding Sites (MeSH), Glutathione (MeSH), Inactivation, Metabolic (MeSH), Mass Spectrometry (MeSH), Metalloproteins (chemistry), Metalloproteins (metabolism), Phytochelatins (MeSH), Plant Proteins (chemistry), Plant Proteins (metabolism), Plants (drug effects), Plants (metabolism).
- MESH :
- chemical , chemistry : Metalloproteins, Plant Proteins.
- chemical , metabolism : Arsenic, Metalloproteins, Plant Proteins.
- chemical , toxicity : Arsenic.
- drug effects : Plants.
- metabolism : Plants.
- Binding Sites, Glutathione, Inactivation, Metabolic, Mass Spectrometry, Phytochelatins.
Abstract
As is a ubiquitous element present in the atmosphere as well as in the aquatic and terrestrial environments. Arsenite and arsenate are the major forms of As intoxication, and these anions are readily taken up by plants. Both anions efficiently induce the biosynthesis of phytochelatins (PCs) ([gamma-glutamate-cysteine](n)-glycine) in vivo and in vitro. The rapid induction of the metal-binding PCs has been observed in cell suspension cultures of Rauvolfia serpentina, in seedlings of Arabidopsis, and in enzyme preparations of Silene vulgaris upon challenge to arsenicals. The rate of PC formation in enzyme preparations was lower compared with Cd-induced biosynthesis, but was accompanied by a prolonged induction phase that resulted finally in higher peptide levels. An approximately 3:1 ratio of the sulfhydryl groups from PCs to As is compatible with reported As-glutathione complexes. The identity of the As-induced PCs and of reconstituted metal-peptide complexes has unequivocally been demonstrated by electrospray ionization mass spectroscopy. Gel filtration experiments and inhibitor studies also indicate a complexation and detoxification of As by the induced PCs.
DOI: 10.1104/pp.122.3.793
PubMed: 10712543
PubMed Central: PMC58915
Links to Exploration step
pubmed:10712543Le document en format XML
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Grill</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10712543</idno><idno type="pmid">10712543</idno><idno type="pmc">PMC58915</idno><idno type="doi">10.1104/pp.122.3.793</idno><idno type="wicri:Area/Main/Corpus">000440</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000440</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detoxification of arsenic by phytochelatins in plants.</title><author><name sortKey="Schmoger, M E" sort="Schmoger, M E" uniqKey="Schmoger M" first="M E" last="Schmöger">M E Schmöger</name><affiliation><nlm:affiliation>Lehrstuhl für Botanik, Technische Universität München, Biologikum-Weihenstephan, Am Hochanger 4, 85350 Freising, Germany.</nlm:affiliation></affiliation></author><author><name sortKey="Oven, M" sort="Oven, M" uniqKey="Oven M" first="M" last="Oven">M. Oven</name></author><author><name sortKey="Grill, E" sort="Grill, E" uniqKey="Grill E" first="E" last="Grill">E. Grill</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arsenic (metabolism)</term><term>Arsenic (toxicity)</term><term>Binding Sites (MeSH)</term><term>Glutathione (MeSH)</term><term>Inactivation, Metabolic (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Metalloproteins (chemistry)</term><term>Metalloproteins (metabolism)</term><term>Phytochelatins (MeSH)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Plants (drug effects)</term><term>Plants (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Metalloproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Arsenic</term><term>Metalloproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Arsenic</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Binding Sites</term><term>Glutathione</term><term>Inactivation, Metabolic</term><term>Mass Spectrometry</term><term>Phytochelatins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">As is a ubiquitous element present in the atmosphere as well as in the aquatic and terrestrial environments. Arsenite and arsenate are the major forms of As intoxication, and these anions are readily taken up by plants. Both anions efficiently induce the biosynthesis of phytochelatins (PCs) ([gamma-glutamate-cysteine](n)-glycine) in vivo and in vitro. The rapid induction of the metal-binding PCs has been observed in cell suspension cultures of Rauvolfia serpentina, in seedlings of Arabidopsis, and in enzyme preparations of Silene vulgaris upon challenge to arsenicals. The rate of PC formation in enzyme preparations was lower compared with Cd-induced biosynthesis, but was accompanied by a prolonged induction phase that resulted finally in higher peptide levels. An approximately 3:1 ratio of the sulfhydryl groups from PCs to As is compatible with reported As-glutathione complexes. The identity of the As-induced PCs and of reconstituted metal-peptide complexes has unequivocally been demonstrated by electrospray ionization mass spectroscopy. Gel filtration experiments and inhibitor studies also indicate a complexation and detoxification of As by the induced PCs.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">10712543</PMID><DateCompleted><Year>2000</Year><Month>05</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>14</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>122</Volume><Issue>3</Issue><PubDate><Year>2000</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Detoxification of arsenic by phytochelatins in plants.</ArticleTitle><Pagination><MedlinePgn>793-801</MedlinePgn></Pagination><Abstract><AbstractText>As is a ubiquitous element present in the atmosphere as well as in the aquatic and terrestrial environments. Arsenite and arsenate are the major forms of As intoxication, and these anions are readily taken up by plants. Both anions efficiently induce the biosynthesis of phytochelatins (PCs) ([gamma-glutamate-cysteine](n)-glycine) in vivo and in vitro. The rapid induction of the metal-binding PCs has been observed in cell suspension cultures of Rauvolfia serpentina, in seedlings of Arabidopsis, and in enzyme preparations of Silene vulgaris upon challenge to arsenicals. The rate of PC formation in enzyme preparations was lower compared with Cd-induced biosynthesis, but was accompanied by a prolonged induction phase that resulted finally in higher peptide levels. An approximately 3:1 ratio of the sulfhydryl groups from PCs to As is compatible with reported As-glutathione complexes. The identity of the As-induced PCs and of reconstituted metal-peptide complexes has unequivocally been demonstrated by electrospray ionization mass spectroscopy. Gel filtration experiments and inhibitor studies also indicate a complexation and detoxification of As by the induced PCs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Schmöger</LastName><ForeName>M E</ForeName><Initials>ME</Initials><AffiliationInfo><Affiliation>Lehrstuhl für Botanik, Technische Universität München, Biologikum-Weihenstephan, Am Hochanger 4, 85350 Freising, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Oven</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Grill</LastName><ForeName>E</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D008667">Metalloproteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>98726-08-0</RegistryNumber><NameOfSubstance UI="D054811">Phytochelatins</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical><Chemical><RegistryNumber>N712M78A8G</RegistryNumber><NameOfSubstance UI="D001151">Arsenic</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001151" MajorTopicYN="N">Arsenic</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000633" 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