Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.
Identifieur interne : 000412 ( Main/Corpus ); précédent : 000411; suivant : 000413Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.
Auteurs : W. Bae ; R K Mehra ; A. Mulchandani ; W. ChenSource :
- Applied and environmental microbiology [ 0099-2240 ] ; 2001.
English descriptors
- KwdEn :
- Chelating Agents (chemical synthesis), Chelating Agents (metabolism), Escherichia coli (genetics), Escherichia coli (growth & development), Escherichia coli (metabolism), Genetic Engineering (methods), Glutathione (MeSH), Mercury (metabolism), Metalloproteins (chemical synthesis), Metalloproteins (genetics), Metalloproteins (metabolism), Phytochelatins (MeSH), Plant Proteins (chemical synthesis), Plant Proteins (genetics), Plant Proteins (metabolism).
- MESH :
- chemical , chemical synthesis : Chelating Agents, Metalloproteins, Plant Proteins.
- chemical , genetics : Metalloproteins, Plant Proteins.
- chemical , metabolism : Chelating Agents, Mercury, Metalloproteins, Plant Proteins.
- genetics : Escherichia coli.
- growth & development : Escherichia coli.
- metabolism : Escherichia coli.
- methods : Genetic Engineering.
- chemical : Glutathione, Phytochelatins.
Abstract
Synthetic phytochelatins (ECs) are a new class of metal-binding peptides with a repetitive metal-binding motif, (Glu-Cys)(n)Gly, which were shown to bind heavy metals more effectively than metallothioneins. However, the limited uptake across the cell membrane is often the rate-limiting factor for the intracellular bioaccumulation of heavy metals by genetically engineered organisms expressing these metal-binding peptides. In this paper, two potential solutions were investigated to overcome this uptake limitation either by coexpressing an Hg(2+) transport system with (Glu-Cys)(20)Gly (EC20) or by directly expressing EC20 on the cell surface. Both approaches were equally effective in increasing the bioaccumulation of Hg(2+). Since the available transport systems are presently limited to only a few heavy metals, our results suggest that bioaccumulation by bacterial sorbents with surface-expressed metal-binding peptides may be useful as a universal strategy for the cleanup of heavy metal contamination.
DOI: 10.1128/AEM.67.11.5335-5338.2001
PubMed: 11679366
PubMed Central: PMC93311
Links to Exploration step
pubmed:11679366Le document en format XML
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Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2001">2001</date><idno type="RBID">pubmed:11679366</idno><idno type="pmid">11679366</idno><idno type="doi">10.1128/AEM.67.11.5335-5338.2001</idno><idno type="pmc">PMC93311</idno><idno type="wicri:Area/Main/Corpus">000412</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000412</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.</title><author><name sortKey="Bae, W" sort="Bae, W" uniqKey="Bae W" first="W" last="Bae">W. Bae</name><affiliation><nlm:affiliation>Department of Chemical and Environmental Engineering, University of California, Riverside, California 92521, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Mehra, R K" sort="Mehra, R K" uniqKey="Mehra R" first="R K" last="Mehra">R K Mehra</name></author><author><name sortKey="Mulchandani, A" sort="Mulchandani, A" uniqKey="Mulchandani A" first="A" last="Mulchandani">A. Mulchandani</name></author><author><name sortKey="Chen, W" sort="Chen, W" uniqKey="Chen W" first="W" last="Chen">W. Chen</name></author></analytic><series><title level="j">Applied and environmental microbiology</title><idno type="ISSN">0099-2240</idno><imprint><date when="2001" type="published">2001</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chelating Agents (chemical synthesis)</term><term>Chelating Agents (metabolism)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (growth & development)</term><term>Escherichia coli (metabolism)</term><term>Genetic Engineering (methods)</term><term>Glutathione (MeSH)</term><term>Mercury (metabolism)</term><term>Metalloproteins (chemical synthesis)</term><term>Metalloproteins (genetics)</term><term>Metalloproteins (metabolism)</term><term>Phytochelatins (MeSH)</term><term>Plant Proteins (chemical synthesis)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Chelating Agents</term><term>Metalloproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metalloproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chelating Agents</term><term>Mercury</term><term>Metalloproteins</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Glutathione</term><term>Phytochelatins</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Synthetic phytochelatins (ECs) are a new class of metal-binding peptides with a repetitive metal-binding motif, (Glu-Cys)(n)Gly, which were shown to bind heavy metals more effectively than metallothioneins. However, the limited uptake across the cell membrane is often the rate-limiting factor for the intracellular bioaccumulation of heavy metals by genetically engineered organisms expressing these metal-binding peptides. In this paper, two potential solutions were investigated to overcome this uptake limitation either by coexpressing an Hg(2+) transport system with (Glu-Cys)(20)Gly (EC20) or by directly expressing EC20 on the cell surface. Both approaches were equally effective in increasing the bioaccumulation of Hg(2+). Since the available transport systems are presently limited to only a few heavy metals, our results suggest that bioaccumulation by bacterial sorbents with surface-expressed metal-binding peptides may be useful as a universal strategy for the cleanup of heavy metal contamination.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11679366</PMID><DateCompleted><Year>2002</Year><Month>01</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0099-2240</ISSN><JournalIssue CitedMedium="Print"><Volume>67</Volume><Issue>11</Issue><PubDate><Year>2001</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Applied and environmental microbiology</Title><ISOAbbreviation>Appl Environ Microbiol</ISOAbbreviation></Journal><ArticleTitle>Genetic engineering of Escherichia coli for enhanced uptake and bioaccumulation of mercury.</ArticleTitle><Pagination><MedlinePgn>5335-8</MedlinePgn></Pagination><Abstract><AbstractText>Synthetic phytochelatins (ECs) are a new class of metal-binding peptides with a repetitive metal-binding motif, (Glu-Cys)(n)Gly, which were shown to bind heavy metals more effectively than metallothioneins. However, the limited uptake across the cell membrane is often the rate-limiting factor for the intracellular bioaccumulation of heavy metals by genetically engineered organisms expressing these metal-binding peptides. In this paper, two potential solutions were investigated to overcome this uptake limitation either by coexpressing an Hg(2+) transport system with (Glu-Cys)(20)Gly (EC20) or by directly expressing EC20 on the cell surface. Both approaches were equally effective in increasing the bioaccumulation of Hg(2+). 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MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>10</Month><Day>27</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2002</Year><Month>1</Month><Day>18</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>10</Month><Day>27</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11679366</ArticleId><ArticleId IdType="doi">10.1128/AEM.67.11.5335-5338.2001</ArticleId><ArticleId IdType="pmc">PMC93311</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>J Bacteriol. 1998 May;180(9):2280-4</Citation><ArticleIdList><ArticleId 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