Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.
Identifieur interne : 000104 ( Main/Corpus ); précédent : 000103; suivant : 000105Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.
Auteurs : Lan-Song Li ; Yu-Ping Meng ; Qiu-Fen Cao ; Yong-Zhen Yang ; Fan Wang ; Hu-Sheng Jia ; Shu-Biao Wu ; Xu-Guang LiuSource :
- Biochemistry. Biokhimiia [ 1608-3040 ] ; 2016.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Cadmium (metabolism), Cadmium (toxicity), Cloning, Molecular (MeSH), DNA, Complementary (genetics), DNA, Complementary (metabolism), Electrophoresis, Polyacrylamide Gel (MeSH), Escherichia coli (drug effects), Escherichia coli (metabolism), Metallothionein (chemistry), Metallothionein (genetics), Metallothionein (metabolism), Metals, Heavy (metabolism), Metals, Heavy (toxicity), Molecular Sequence Data (MeSH), Plasmids (genetics), Plasmids (metabolism), Real-Time Polymerase Chain Reaction (MeSH), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (chemistry), Recombinant Fusion Proteins (isolation & purification), Sequence Alignment (MeSH), Ziziphus (drug effects), Ziziphus (metabolism).
- MESH :
- chemical , biosynthesis : Recombinant Fusion Proteins.
- chemical , chemistry : Metallothionein, Recombinant Fusion Proteins.
- chemical , genetics : DNA, Complementary, Metallothionein.
- chemical , isolation & purification : Recombinant Fusion Proteins.
- chemical , metabolism : Cadmium, DNA, Complementary, Metallothionein, Metals, Heavy.
- chemical , toxicity : Cadmium, Metals, Heavy.
- drug effects : Escherichia coli, Ziziphus.
- genetics : Plasmids.
- metabolism : Escherichia coli, Plasmids, Ziziphus.
- Amino Acid Sequence, Cloning, Molecular, Electrophoresis, Polyacrylamide Gel, Molecular Sequence Data, Real-Time Polymerase Chain Reaction, Sequence Alignment.
Abstract
Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater.
DOI: 10.1134/S000629791606002X
PubMed: 27301284
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.</title><author><name sortKey="Li, Lan Song" sort="Li, Lan Song" uniqKey="Li L" first="Lan-Song" last="Li">Lan-Song Li</name><affiliation><nlm:affiliation>College of Chemistry and Chemical Engineering, Taiyuan University of Technology, 030024 Taiyuan, China. liuxuguang@tyut.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Meng, Yu Ping" sort="Meng, Yu Ping" uniqKey="Meng Y" first="Yu-Ping" last="Meng">Yu-Ping Meng</name></author><author><name sortKey="Cao, Qiu Fen" sort="Cao, Qiu Fen" uniqKey="Cao Q" first="Qiu-Fen" last="Cao">Qiu-Fen Cao</name></author><author><name sortKey="Yang, Yong Zhen" sort="Yang, Yong Zhen" uniqKey="Yang Y" first="Yong-Zhen" last="Yang">Yong-Zhen Yang</name></author><author><name sortKey="Wang, Fan" sort="Wang, Fan" uniqKey="Wang F" first="Fan" last="Wang">Fan Wang</name></author><author><name sortKey="Jia, Hu Sheng" sort="Jia, Hu Sheng" uniqKey="Jia H" first="Hu-Sheng" last="Jia">Hu-Sheng Jia</name></author><author><name sortKey="Wu, Shu Biao" sort="Wu, Shu Biao" uniqKey="Wu S" first="Shu-Biao" last="Wu">Shu-Biao Wu</name></author><author><name sortKey="Liu, Xu Guang" sort="Liu, Xu Guang" uniqKey="Liu X" first="Xu-Guang" last="Liu">Xu-Guang Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:27301284</idno><idno type="pmid">27301284</idno><idno type="doi">10.1134/S000629791606002X</idno><idno type="wicri:Area/Main/Corpus">000104</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000104</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.</title><author><name sortKey="Li, Lan Song" sort="Li, Lan Song" uniqKey="Li L" first="Lan-Song" last="Li">Lan-Song Li</name><affiliation><nlm:affiliation>College of Chemistry and Chemical Engineering, Taiyuan University of Technology, 030024 Taiyuan, China. liuxuguang@tyut.edu.cn.</nlm:affiliation></affiliation></author><author><name sortKey="Meng, Yu Ping" sort="Meng, Yu Ping" uniqKey="Meng Y" first="Yu-Ping" last="Meng">Yu-Ping Meng</name></author><author><name sortKey="Cao, Qiu Fen" sort="Cao, Qiu Fen" uniqKey="Cao Q" first="Qiu-Fen" last="Cao">Qiu-Fen Cao</name></author><author><name sortKey="Yang, Yong Zhen" sort="Yang, Yong Zhen" uniqKey="Yang Y" first="Yong-Zhen" last="Yang">Yong-Zhen Yang</name></author><author><name sortKey="Wang, Fan" sort="Wang, Fan" uniqKey="Wang F" first="Fan" last="Wang">Fan Wang</name></author><author><name sortKey="Jia, Hu Sheng" sort="Jia, Hu Sheng" uniqKey="Jia H" first="Hu-Sheng" last="Jia">Hu-Sheng Jia</name></author><author><name sortKey="Wu, Shu Biao" sort="Wu, Shu Biao" uniqKey="Wu S" first="Shu-Biao" last="Wu">Shu-Biao Wu</name></author><author><name sortKey="Liu, Xu Guang" sort="Liu, Xu Guang" uniqKey="Liu X" first="Xu-Guang" last="Liu">Xu-Guang Liu</name></author></analytic><series><title level="j">Biochemistry. Biokhimiia</title><idno type="eISSN">1608-3040</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Cadmium (metabolism)</term><term>Cadmium (toxicity)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (metabolism)</term><term>Electrophoresis, Polyacrylamide Gel (MeSH)</term><term>Escherichia coli (drug effects)</term><term>Escherichia coli (metabolism)</term><term>Metallothionein (chemistry)</term><term>Metallothionein (genetics)</term><term>Metallothionein (metabolism)</term><term>Metals, Heavy (metabolism)</term><term>Metals, Heavy (toxicity)</term><term>Molecular Sequence Data (MeSH)</term><term>Plasmids (genetics)</term><term>Plasmids (metabolism)</term><term>Real-Time Polymerase Chain Reaction (MeSH)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (chemistry)</term><term>Recombinant Fusion Proteins (isolation & purification)</term><term>Sequence Alignment (MeSH)</term><term>Ziziphus (drug effects)</term><term>Ziziphus (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Metallothionein</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>Metallothionein</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term><term>DNA, Complementary</term><term>Metallothionein</term><term>Metals, Heavy</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term><term>Metals, Heavy</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term><term>Ziziphus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Plasmids</term><term>Ziziphus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Molecular Sequence Data</term><term>Real-Time Polymerase Chain Reaction</term><term>Sequence Alignment</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27301284</PMID><DateCompleted><Year>2017</Year><Month>01</Month><Day>11</Day></DateCompleted><DateRevised><Year>2017</Year><Month>01</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1608-3040</ISSN><JournalIssue CitedMedium="Internet"><Volume>81</Volume><Issue>6</Issue><PubDate><Year>2016</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Biochemistry. Biokhimiia</Title><ISOAbbreviation>Biochemistry (Mosc)</ISOAbbreviation></Journal><ArticleTitle>Type 1 Metallothionein (ZjMT) Is Responsible for Heavy Metal Tolerance in Ziziphus jujuba.</ArticleTitle><Pagination><MedlinePgn>565-73</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1134/S000629791606002X</ELocationID><Abstract><AbstractText>Metallothioneins (MTs) are a family of low molecular weight, cysteine-rich, metal-binding proteins that are able to make cells to uptake heavy metals from the environment. Molecular and functional characterization of this gene family improves understanding of the mechanisms underlying heavy metal tolerance in higher organisms. In this study, a cDNA clone, encoding 74-a.a. metallothionein type 1 protein (ZjMT), was isolated from the cDNA library of Ziziphus jujuba. At the N- and C-terminals of the deduced amino acid sequence of ZjMT, six cysteine residues were arranged in a CXCXXXCXCXXXCXC and CXCXXXCXCXXCXC structure, respectively, indicating that ZjMT is a type 1 MT. Quantitative PCR analysis of plants subjected to cadmium stress showed enhanced expression of ZjMT gene in Z. jujuba within 24 h upon Cd exposure. Escherichia coli cells expressing ZjMT exhibited enhanced metal tolerance and higher accumulation of metal ions compared with control cells. The results indicate that ZjMT contributes to the detoxification of metal ions and provides marked tolerance against metal stresses. Therefore, ZjMT may be a potential candidate for tolerance enhancement in vulnerable plants to heavy metal stress and E. coli cells containing the ZjMT gene may be applied to adsorb heavy metals in polluted wastewater. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Lan-Song</ForeName><Initials>LS</Initials><AffiliationInfo><Affiliation>College of Chemistry and Chemical Engineering, Taiyuan University of Technology, 030024 Taiyuan, China. liuxuguang@tyut.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>Yu-Ping</ForeName><Initials>YP</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Qiu-Fen</ForeName><Initials>QF</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Yong-Zhen</ForeName><Initials>YZ</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Fan</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Hu-Sheng</ForeName><Initials>HS</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Shu-Biao</ForeName><Initials>SB</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xu-Guang</ForeName><Initials>XG</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biochemistry (Mosc)</MedlineTA><NlmUniqueID>0376536</NlmUniqueID><ISSNLinking>0006-2979</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019216">Metals, Heavy</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>9038-94-2</RegistryNumber><NameOfSubstance UI="D008668">Metallothionein</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008668" MajorTopicYN="N">Metallothionein</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019216" MajorTopicYN="N">Metals, Heavy</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence 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