Characterization analysis and heavy metal-binding properties of CsMTL3 in Escherichia coli.
Identifieur interne : 000043 ( Main/Corpus ); précédent : 000042; suivant : 000044Characterization analysis and heavy metal-binding properties of CsMTL3 in Escherichia coli.
Auteurs : Xing Xu ; Ling Duan ; Jingwen Yu ; Chenggang Su ; Jinhua Li ; Dan Chen ; Xingguo Zhang ; Hongyuan Song ; Yu PanSource :
- FEBS open bio [ 2211-5463 ] ; 2018.
Abstract
Members of the metallothionein (MT) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, CsMTL3, was characterized from a complementary DNA (cDNA) library from young cucumber fruit; CsMTL3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N-terminal Cys residues was different from that of MT-like 2. Heterologous expression of CsMTL3 in Escherichia coli improved their heavy metal tolerance, particularly to Cd2+ and Cu2+, and led to increased uptake of Cd2+ and Cu2+; increased uptake was also observed for cells expressing Arabidopsis thaliana metallothionein 3 (AtMT3) and phytochelatin-like (PCL), with greatest uptake in PCL-expressing cells. These findings demonstrate that CsMTL3 can improve metal tolerance, especially for Cd2+ ions, when heterologously expressed in E. coli, and suggest that the composition and arrangement of N-terminal Cys residues are associated with binding capacity and preference for different metal ions.
DOI: 10.1002/2211-5463.12520
PubMed: 30410861
PubMed Central: PMC6212650
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Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Jingwen" sort="Yu, Jingwen" uniqKey="Yu J" first="Jingwen" last="Yu">Jingwen Yu</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Su, Chenggang" sort="Su, Chenggang" uniqKey="Su C" first="Chenggang" last="Su">Chenggang Su</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jinhua" sort="Li, Jinhua" uniqKey="Li J" first="Jinhua" last="Li">Jinhua Li</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Dan" sort="Chen, Dan" uniqKey="Chen D" first="Dan" last="Chen">Dan Chen</name><affiliation><nlm:affiliation>Comprehensive Testing Center of Guangzhou Entry-Exit Inspection & Quarantine Bureau Guangzhou China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Xingguo" sort="Zhang, Xingguo" uniqKey="Zhang X" first="Xingguo" last="Zhang">Xingguo Zhang</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern 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Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Duan, Ling" sort="Duan, Ling" uniqKey="Duan L" first="Ling" last="Duan">Ling Duan</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Jingwen" sort="Yu, Jingwen" uniqKey="Yu J" first="Jingwen" last="Yu">Jingwen Yu</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Su, Chenggang" sort="Su, Chenggang" uniqKey="Su C" first="Chenggang" last="Su">Chenggang Su</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Jinhua" sort="Li, Jinhua" uniqKey="Li J" first="Jinhua" last="Li">Jinhua Li</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Chen, Dan" sort="Chen, Dan" uniqKey="Chen D" first="Dan" last="Chen">Dan Chen</name><affiliation><nlm:affiliation>Comprehensive Testing Center of Guangzhou Entry-Exit Inspection & Quarantine Bureau Guangzhou China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Xingguo" sort="Zhang, Xingguo" uniqKey="Zhang X" first="Xingguo" last="Zhang">Xingguo Zhang</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Song, Hongyuan" sort="Song, Hongyuan" uniqKey="Song H" first="Hongyuan" last="Song">Hongyuan Song</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author><author><name sortKey="Pan, Yu" sort="Pan, Yu" uniqKey="Pan Y" first="Yu" last="Pan">Yu Pan</name><affiliation><nlm:affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">FEBS open bio</title><idno type="ISSN">2211-5463</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Members of the metallothionein (MT) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, <i>CsMTL3</i>, was characterized from a complementary DNA (cDNA) library from young cucumber fruit; CsMTL3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N-terminal Cys residues was different from that of MT-like 2. Heterologous expression of <i>CsMTL3</i> in <i>Escherichia coli</i> improved their heavy metal tolerance, particularly to Cd<sup>2+</sup> and Cu<sup>2+</sup>, and led to increased uptake of Cd<sup>2+</sup> and Cu<sup>2+</sup>; increased uptake was also observed for cells expressing <i>Arabidopsis thaliana</i> metallothionein 3 (AtMT3) and phytochelatin-like (PCL), with greatest uptake in PCL-expressing cells. These findings demonstrate that <i>CsMTL3</i> can improve metal tolerance, especially for Cd<sup>2+</sup> ions, when heterologously expressed in <i>E. coli</i>, and suggest that the composition and arrangement of N-terminal Cys residues are associated with binding capacity and preference for different metal ions.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30410861</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2211-5463</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>11</Issue><PubDate><Year>2018</Year><Month>Nov</Month></PubDate></JournalIssue><Title>FEBS open bio</Title><ISOAbbreviation>FEBS Open Bio</ISOAbbreviation></Journal><ArticleTitle>Characterization analysis and heavy metal-binding properties of <i>CsMTL3</i> in <i>Escherichia coli</i>.</ArticleTitle><Pagination><MedlinePgn>1820-1829</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/2211-5463.12520</ELocationID><Abstract><AbstractText>Members of the metallothionein (MT) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, <i>CsMTL3</i>, was characterized from a complementary DNA (cDNA) library from young cucumber fruit; CsMTL3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N-terminal Cys residues was different from that of MT-like 2. Heterologous expression of <i>CsMTL3</i> in <i>Escherichia coli</i> improved their heavy metal tolerance, particularly to Cd<sup>2+</sup> and Cu<sup>2+</sup>, and led to increased uptake of Cd<sup>2+</sup> and Cu<sup>2+</sup>; increased uptake was also observed for cells expressing <i>Arabidopsis thaliana</i> metallothionein 3 (AtMT3) and phytochelatin-like (PCL), with greatest uptake in PCL-expressing cells. These findings demonstrate that <i>CsMTL3</i> can improve metal tolerance, especially for Cd<sup>2+</sup> ions, when heterologously expressed in <i>E. coli</i>, and suggest that the composition and arrangement of N-terminal Cys residues are associated with binding capacity and preference for different metal ions.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Xing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Duan</LastName><ForeName>Ling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Jingwen</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Su</LastName><ForeName>Chenggang</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Jinhua</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Dan</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Comprehensive Testing Center of Guangzhou Entry-Exit Inspection & Quarantine Bureau Guangzhou China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xingguo</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Hongyuan</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Yu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Horticulture Science for Southern Mountainous Regions Ministry of Education Southwest University Chongqing China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Horticulture and Landscape Architecture Southwest University Chongqing China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>09</Month><Day>19</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Open Bio</MedlineTA><NlmUniqueID>101580716</NlmUniqueID><ISSNLinking>2211-5463</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">CsMTL3</Keyword><Keyword MajorTopicYN="N">Escherichia coli</Keyword><Keyword MajorTopicYN="N">heavy metal tolerance</Keyword><Keyword MajorTopicYN="N">heavy metal‐binding</Keyword><Keyword MajorTopicYN="N">metallothionein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>06</Month><Day>04</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>08</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>08</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>11</Month><Day>10</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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