Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini.
Identifieur interne : 000047 ( PubMed/Curation ); précédent : 000046; suivant : 000048Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini.
Auteurs : Claire Anderson [Australie] ; Muhammad Adil Khan [Australie] ; Ann-Maree Catanzariti [Australie] ; Cameron A. Jack [Australie] ; Adnane Nemri [Australie, Allemagne] ; Gregory J. Lawrence [Australie] ; Narayana M. Upadhyaya [Australie] ; Adrienne R. Hardham [Australie] ; Jeffrey G. Ellis [Australie] ; Peter N. Dodds [Australie] ; David A. Jones [Australie]Source :
- BMC genomics [ 1471-2164 ] ; 2016.
Descripteurs français
- KwdFr :
- Basidiomycota (génétique), Basidiomycota (pathogénicité), Biologie informatique (méthodes), Cartographie chromosomique (MeSH), Fréquence d'allèle (MeSH), Génome fongique (MeSH), Génomique (méthodes), Locus génétiques (MeSH), Mutation (MeSH), Perte d'hétérozygotie (MeSH), Phénotype (MeSH), Polymorphisme de nucléotide simple (MeSH), Recombinaison génétique (MeSH), Séquence d'acides aminés (MeSH), Séquençage nucléotidique à haut débit (MeSH), Virulence (génétique).
- MESH :
- génétique : Basidiomycota, Virulence.
- méthodes : Biologie informatique, Génomique.
- pathogénicité : Basidiomycota.
- Cartographie chromosomique, Fréquence d'allèle, Génome fongique, Locus génétiques, Mutation, Perte d'hétérozygotie, Phénotype, Polymorphisme de nucléotide simple, Recombinaison génétique, Séquence d'acides aminés, Séquençage nucléotidique à haut débit.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Basidiomycota (genetics), Basidiomycota (pathogenicity), Chromosome Mapping (MeSH), Computational Biology (methods), Gene Frequency (MeSH), Genetic Loci (MeSH), Genome, Fungal (MeSH), Genomics (methods), High-Throughput Nucleotide Sequencing (MeSH), Loss of Heterozygosity (MeSH), Mutation (MeSH), Phenotype (MeSH), Polymorphism, Single Nucleotide (MeSH), Recombination, Genetic (MeSH), Virulence (genetics).
- MESH :
- genetics : Basidiomycota, Virulence.
- methods : Computational Biology, Genomics.
- pathogenicity : Basidiomycota.
- Amino Acid Sequence, Chromosome Mapping, Gene Frequency, Genetic Loci, Genome, Fungal, High-Throughput Nucleotide Sequencing, Loss of Heterozygosity, Mutation, Phenotype, Polymorphism, Single Nucleotide, Recombination, Genetic.
Abstract
BACKGROUND
Rust fungi are an important group of plant pathogens that cause devastating losses in agricultural, silvicultural and natural ecosystems. Plants can be protected from rust disease by resistance genes encoding receptors that trigger a highly effective defence response upon recognition of specific pathogen avirulence proteins. Identifying avirulence genes is crucial for understanding how virulence evolves in the field.
RESULTS
To facilitate avirulence gene cloning in the flax rust fungus, Melampsora lini, we constructed a high-density genetic linkage map using single nucleotide polymorphisms detected in restriction site-associated DNA sequencing (RADseq) data. The map comprises 13,412 RADseq markers in 27 linkage groups that together span 5860 cM and contain 2756 recombination bins. The marker sequences were used to anchor 68.9 % of the M. lini genome assembly onto the genetic map. The map and anchored assembly were then used to: 1) show that M. lini has a high overall meiotic recombination rate, but recombination distribution is uneven and large coldspots exist; 2) show that substantial genome rearrangements have occurred in spontaneous loss-of-avirulence mutants; and 3) identify the AvrL2 and AvrM14 avirulence genes by map-based cloning. AvrM14 is a dual-specificity avirulence gene that encodes a predicted nudix hydrolase. AvrL2 is located in the region of the M. lini genome with the lowest recombination rate and encodes a small, highly-charged proline-rich protein.
CONCLUSIONS
The M. lini high-density linkage map has greatly advanced our understanding of virulence mechanisms in this pathogen by providing novel insights into genome variability and enabling identification of two new avirulence genes.
DOI: 10.1186/s12864-016-3011-9
PubMed: 27550217
PubMed Central: PMC4994203
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<term>Basidiomycota (genetics)</term>
<term>Basidiomycota (pathogenicity)</term>
<term>Chromosome Mapping (MeSH)</term>
<term>Computational Biology (methods)</term>
<term>Gene Frequency (MeSH)</term>
<term>Genetic Loci (MeSH)</term>
<term>Genome, Fungal (MeSH)</term>
<term>Genomics (methods)</term>
<term>High-Throughput Nucleotide Sequencing (MeSH)</term>
<term>Loss of Heterozygosity (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Phenotype (MeSH)</term>
<term>Polymorphism, Single Nucleotide (MeSH)</term>
<term>Recombination, Genetic (MeSH)</term>
<term>Virulence (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Basidiomycota (génétique)</term>
<term>Basidiomycota (pathogénicité)</term>
<term>Biologie informatique (méthodes)</term>
<term>Cartographie chromosomique (MeSH)</term>
<term>Fréquence d'allèle (MeSH)</term>
<term>Génome fongique (MeSH)</term>
<term>Génomique (méthodes)</term>
<term>Locus génétiques (MeSH)</term>
<term>Mutation (MeSH)</term>
<term>Perte d'hétérozygotie (MeSH)</term>
<term>Phénotype (MeSH)</term>
<term>Polymorphisme de nucléotide simple (MeSH)</term>
<term>Recombinaison génétique (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Séquençage nucléotidique à haut débit (MeSH)</term>
<term>Virulence (génétique)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Basidiomycota</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Computational Biology</term>
<term>Genomics</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biologie informatique</term>
<term>Génomique</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Chromosome Mapping</term>
<term>Gene Frequency</term>
<term>Genetic Loci</term>
<term>Genome, Fungal</term>
<term>High-Throughput Nucleotide Sequencing</term>
<term>Loss of Heterozygosity</term>
<term>Mutation</term>
<term>Phenotype</term>
<term>Polymorphism, Single Nucleotide</term>
<term>Recombination, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cartographie chromosomique</term>
<term>Fréquence d'allèle</term>
<term>Génome fongique</term>
<term>Locus génétiques</term>
<term>Mutation</term>
<term>Perte d'hétérozygotie</term>
<term>Phénotype</term>
<term>Polymorphisme de nucléotide simple</term>
<term>Recombinaison génétique</term>
<term>Séquence d'acides aminés</term>
<term>Séquençage nucléotidique à haut débit</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Rust fungi are an important group of plant pathogens that cause devastating losses in agricultural, silvicultural and natural ecosystems. Plants can be protected from rust disease by resistance genes encoding receptors that trigger a highly effective defence response upon recognition of specific pathogen avirulence proteins. Identifying avirulence genes is crucial for understanding how virulence evolves in the field.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>To facilitate avirulence gene cloning in the flax rust fungus, Melampsora lini, we constructed a high-density genetic linkage map using single nucleotide polymorphisms detected in restriction site-associated DNA sequencing (RADseq) data. The map comprises 13,412 RADseq markers in 27 linkage groups that together span 5860 cM and contain 2756 recombination bins. The marker sequences were used to anchor 68.9 % of the M. lini genome assembly onto the genetic map. The map and anchored assembly were then used to: 1) show that M. lini has a high overall meiotic recombination rate, but recombination distribution is uneven and large coldspots exist; 2) show that substantial genome rearrangements have occurred in spontaneous loss-of-avirulence mutants; and 3) identify the AvrL2 and AvrM14 avirulence genes by map-based cloning. AvrM14 is a dual-specificity avirulence gene that encodes a predicted nudix hydrolase. AvrL2 is located in the region of the M. lini genome with the lowest recombination rate and encodes a small, highly-charged proline-rich protein.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The M. lini high-density linkage map has greatly advanced our understanding of virulence mechanisms in this pathogen by providing novel insights into genome variability and enabling identification of two new avirulence genes.</p>
</div>
</front>
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<Day>11</Day>
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<DateRevised><Year>2018</Year>
<Month>11</Month>
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<Month>08</Month>
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<Title>BMC genomics</Title>
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<ArticleTitle>Genome analysis and avirulence gene cloning using a high-density RADseq linkage map of the flax rust fungus, Melampsora lini.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12864-016-3011-9</ELocationID>
<Abstract><AbstractText Label="BACKGROUND">Rust fungi are an important group of plant pathogens that cause devastating losses in agricultural, silvicultural and natural ecosystems. Plants can be protected from rust disease by resistance genes encoding receptors that trigger a highly effective defence response upon recognition of specific pathogen avirulence proteins. Identifying avirulence genes is crucial for understanding how virulence evolves in the field.</AbstractText>
<AbstractText Label="RESULTS">To facilitate avirulence gene cloning in the flax rust fungus, Melampsora lini, we constructed a high-density genetic linkage map using single nucleotide polymorphisms detected in restriction site-associated DNA sequencing (RADseq) data. The map comprises 13,412 RADseq markers in 27 linkage groups that together span 5860 cM and contain 2756 recombination bins. The marker sequences were used to anchor 68.9 % of the M. lini genome assembly onto the genetic map. The map and anchored assembly were then used to: 1) show that M. lini has a high overall meiotic recombination rate, but recombination distribution is uneven and large coldspots exist; 2) show that substantial genome rearrangements have occurred in spontaneous loss-of-avirulence mutants; and 3) identify the AvrL2 and AvrM14 avirulence genes by map-based cloning. AvrM14 is a dual-specificity avirulence gene that encodes a predicted nudix hydrolase. AvrL2 is located in the region of the M. lini genome with the lowest recombination rate and encodes a small, highly-charged proline-rich protein.</AbstractText>
<AbstractText Label="CONCLUSIONS">The M. lini high-density linkage map has greatly advanced our understanding of virulence mechanisms in this pathogen by providing novel insights into genome variability and enabling identification of two new avirulence genes.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Anderson</LastName>
<ForeName>Claire</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Research School of Biology, The Australian National University, 134 Linnaeus Way, Acton, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Khan</LastName>
<ForeName>Muhammad Adil</ForeName>
<Initials>MA</Initials>
<AffiliationInfo><Affiliation>Research School of Biology, The Australian National University, 134 Linnaeus Way, Acton, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Current address: ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Catanzariti</LastName>
<ForeName>Ann-Maree</ForeName>
<Initials>AM</Initials>
<AffiliationInfo><Affiliation>Research School of Biology, The Australian National University, 134 Linnaeus Way, Acton, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jack</LastName>
<ForeName>Cameron A</ForeName>
<Initials>CA</Initials>
<AffiliationInfo><Affiliation>ANU Bioinformatics Consulting Unit, The John Curtin School of Medical Research, The Australian National University, 131 Garran Road, Acton, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Nemri</LastName>
<ForeName>Adnane</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Current address: KWS SAAT SE, Grimsehlstraße 31, Einbeck, 37574, Germany.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lawrence</LastName>
<ForeName>Gregory J</ForeName>
<Initials>GJ</Initials>
<AffiliationInfo><Affiliation>CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Upadhyaya</LastName>
<ForeName>Narayana M</ForeName>
<Initials>NM</Initials>
<AffiliationInfo><Affiliation>CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hardham</LastName>
<ForeName>Adrienne R</ForeName>
<Initials>AR</Initials>
<AffiliationInfo><Affiliation>Research School of Biology, The Australian National University, 134 Linnaeus Way, Acton, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ellis</LastName>
<ForeName>Jeffrey G</ForeName>
<Initials>JG</Initials>
<AffiliationInfo><Affiliation>CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dodds</LastName>
<ForeName>Peter N</ForeName>
<Initials>PN</Initials>
<AffiliationInfo><Affiliation>CSIRO Agriculture, GPO Box 1600, Canberra, ACT, 2601, Australia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jones</LastName>
<ForeName>David A</ForeName>
<Initials>DA</Initials>
<AffiliationInfo><Affiliation>Research School of Biology, The Australian National University, 134 Linnaeus Way, Acton, ACT, 2601, Australia. david.jones@anu.edu.au.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>08</Month>
<Day>22</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Genomics</MedlineTA>
<NlmUniqueID>100965258</NlmUniqueID>
<ISSNLinking>1471-2164</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002874" MajorTopicYN="Y">Chromosome Mapping</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005787" MajorTopicYN="N">Gene Frequency</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D056426" MajorTopicYN="N">Genetic Loci</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016681" MajorTopicYN="Y">Genome, Fungal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D023281" MajorTopicYN="Y">Genomics</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059014" MajorTopicYN="N">High-Throughput Nucleotide Sequencing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019656" MajorTopicYN="N">Loss of Heterozygosity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010641" MajorTopicYN="N">Phenotype</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020641" MajorTopicYN="N">Polymorphism, Single Nucleotide</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011995" MajorTopicYN="N">Recombination, Genetic</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Avirulence gene</Keyword>
<Keyword MajorTopicYN="Y">Genetic linkage map</Keyword>
<Keyword MajorTopicYN="Y">Loss of heterozygosity (LOH)</Keyword>
<Keyword MajorTopicYN="Y">Map-based cloning</Keyword>
<Keyword MajorTopicYN="Y">Melampsora lini</Keyword>
<Keyword MajorTopicYN="Y">Recombination</Keyword>
<Keyword MajorTopicYN="Y">Restriction-site associated DNA sequencing (RADseq)</Keyword>
<Keyword MajorTopicYN="Y">Rust fungus</Keyword>
<Keyword MajorTopicYN="Y">Scaffold anchoring</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>04</Month>
<Day>19</Day>
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<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>08</Month>
<Day>11</Day>
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<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
<Month>8</Month>
<Day>24</Day>
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<Month>9</Month>
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<ArticleId IdType="doi">10.1186/s12864-016-3011-9</ArticleId>
<ArticleId IdType="pii">10.1186/s12864-016-3011-9</ArticleId>
<ArticleId IdType="pmc">PMC4994203</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Mol Syst Biol. 2011 Oct 11;7:539</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21988835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Heredity (Edinb). 2012 Apr;108(4):447-55</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22072068</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>G3 (Bethesda). 2014 Dec 11;5(1):133-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25504737</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mamm Genome. 2001 Apr;12(4):318-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11309665</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2012 Aug;15(4):375-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22658703</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Biol Evol. 2009 Nov;26(11):2499-513</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19633228</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2007 Jul;8(4):349-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20507505</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2013 Sep;25(9):3153-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24038649</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2010;11(11):R109</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21067574</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genes Dev. 2008 Jan 15;22(2):194-211</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18198337</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2015 Dec;16(9):1006-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25784533</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2015 Jan;16(1):18-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25531225</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2014 Sep 11;10(9):e1004329</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25211126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Mol Life Sci. 2006 Jan;63(2):123-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16378245</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2012 Oct;25(10):1350-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22712506</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2008;3(10):e3376</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18852878</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2011 Oct;14(5):512-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21723182</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2011 Aug;7(8):e1002230</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21876677</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2010 Nov 16;11:637</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21080964</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2006 Jan;18(1):243-56</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16326930</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2011 Nov 29;13(1):36-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22124482</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Bioinformatics. 2012 May 24;13:111</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22624656</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2009 Mar;19(3):510-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19088305</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Aug 26;5:422</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25206357</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Biol. 2010 Mar 09;8(3):e1000326</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20231873</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 Aug 30;108(35):14682-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21821794</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2013 Jan;14(1):96-107</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22998218</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Methods. 2011 Sep 29;8(10):785-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21959131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Methods Mol Biol. 2011;772:157-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22065437</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2014 Aug;20:19-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24780462</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2006 Apr;18(4):1038-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16531493</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Pathog. 2011 Nov;7(11):e1002353</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22102810</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Res. 2009 Nov;19(11):1925-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19596977</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1996 Oct;8(10):1773-91</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8914325</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 2004 Mar;16(3):755-68</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14973158</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Genomics. 2010 Apr;11(2):91-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20885817</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Fungal Genet Biol. 2011 Mar;48(3):266-74</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20888926</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2012;8(11):e1003088</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23209441</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2011 Jun;7(6):e1002070</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21695235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2013 Apr 4;496(7443):91-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23535592</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Biol Sci. 2013 Sep 25;280(1771):20131945</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24068360</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2015 Aug;16(6):623-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25346350</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Funct Plant Biol. 2009;36(5):395-408</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21760756</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Oct 31;5:594</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25400655</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2007 Sep 7;317(5843):1400-2</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17823352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant J. 2006 Feb;45(4):616-29</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16441352</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2015 Jan 08;5:759</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25620970</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>G3 (Bethesda). 2011 Aug;1(3):171-82</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22384329</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Microbiol. 2011 Dec;13(12):1849-57</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21848815</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Genet. 2015 Jun 19;6:220</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26150829</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Genet. 2015 Jul;31(7):393-401</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25907025</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Phytopathol. 2011;49:465-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21568701</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1990 Oct 5;215(3):403-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2231712</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011 May 04;6(5):e19379</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21573248</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mycologia. 2010 Nov-Dec;102(6):1295-302</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20943541</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Ecol. 2008 Jul;17(14):3401-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18573166</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Chem Rev. 2014 Jul 9;114(13):6589-631</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24773235</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS Genet. 2008 Oct;4(10):e1000212</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18846212</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1999 Mar;11(3):495-506</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10072407</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Heredity (Edinb). 2016 Apr;116(4):384-94</Citation>
<ArticleIdList><ArticleId IdType="pubmed">26758189</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eukaryot Cell. 2013 Jan;12(1):2-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">22962277</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2009 Feb;22(2):115-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19132864</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Curr Opin Plant Biol. 2012 Nov;15(5):556-61</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23017241</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Genet. 2007 Mar;23(3):134-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17275131</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(8):e24230</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21909385</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 1980 Jul 25;209(4455):501-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17831369</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2011 May 31;108(22):9166-71</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21536894</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2011 Jan;12(1):93-102</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21118351</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Genet. 2014 Jul 07;5:208</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25071835</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2008 Jul 24;454(7203):479-85</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18615017</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytopathology. 2000 Aug;90(8):819-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18944502</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiology. 2005 Sep;151(Pt 9):2873-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16151200</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cold Spring Harb Symp Quant Biol. 2012;77:235-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23223409</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genome Biol. 2006;7 Suppl 1:S10.1-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16925832</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>G3 (Bethesda). 2014 Sep 15;4(11):2219-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25227227</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Genetics. 2005 Nov;171(3):985-1001</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16079234</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Genet. 2013 Mar;14(3):157-67</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23358380</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Theor Appl Genet. 2013 Nov;126(11):2699-716</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23918062</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Plant Sci. 2002 Aug;7(8):352-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12167330</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2011 Feb 15;2:202</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21326234</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genomics. 2014 Aug 23;15:708</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25150411</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Annu Rev Genet. 2013;47:563-99</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24050176</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>BMC Genet. 2014 Apr 11;15:45</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24725999</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Sep 10;5:454</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25309554</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Microbiol Rev. 1995 Dec;59(4):686-98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8531892</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W375-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15215414</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Microbe Interact. 2013 Mar;26(3):330-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23075041</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Plant Pathol. 2010 Jan;11(1):19-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20078773</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Plant Cell. 1997 Apr;9(4):641-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9144966</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Plant Sci. 2014 Mar 24;5:98</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24715894</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Environ Microbiol. 2007 Dec;9(12):2978-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17991027</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>G3 (Bethesda). 2014 Oct 29;4(12):2519-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25360032</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Commun. 2013;4:2673</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24150273</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2013 May 29;8(5):e64799</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23734219</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2006 Nov 2;444(7115):97-101</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17080091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>FEMS Microbiol Lett. 2007 Aug;273(2):125-32</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17610516</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
</record>
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