Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini.
Identifieur interne : 000208 ( PubMed/Corpus ); précédent : 000207; suivant : 000209Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini.
Auteurs : M J Dickinson ; R. Zhang ; A. PryorSource :
- Current genetics [ 0172-8083 ] ; 1993.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Basidiomycota (genetics), Blotting, Northern (MeSH), Cloning, Molecular (MeSH), DNA, Fungal (MeSH), Molecular Sequence Data (MeSH), Open Reading Frames (MeSH), RNA, Double-Stranded (genetics), RNA, Fungal (genetics), Restriction Mapping (MeSH), Sequence Homology, Nucleic Acid (MeSH).
- MESH :
- chemical , genetics : RNA, Double-Stranded, RNA, Fungal.
- chemical : DNA, Fungal.
- genetics : Basidiomycota.
- Amino Acid Sequence, Base Sequence, Blotting, Northern, Cloning, Molecular, Molecular Sequence Data, Open Reading Frames, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
Flax rust, Melampsora lini strain SP6, contains 11 double-stranded (ds) RNA molecules with a total length of about 25 kbp. The dsRNAs are inherited in three genetic units: the L unit comprising a single 5.2 kbp dsRNA and contained within a 40-nm virus-like particle, and the A and B units each consisting of five dsRNAs (A1-A5, and B1-B5) ranging in size from 1.2 to 2.7 kbp. This paper reports the isolation of a cDNA library representing 10 of the 11 dsRNAs. By nucleic-acid hybridization techniques it has been shown that all ten sequences are unique showing no detectable cross-hybridization with any other dsRNA present in the rust. A near full-length sequence of 1932 bp of the B3 dsRNA is reported and contains several open reading frames, the largest of which comprises most of the molecule.
DOI: 10.1007/BF00351852
PubMed: 8299159
Links to Exploration step
pubmed:8299159Le document en format XML
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Pryor</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8299159</idno><idno type="pmid">8299159</idno><idno type="doi">10.1007/BF00351852</idno><idno type="wicri:Area/PubMed/Corpus">000208</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000208</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini.</title><author><name sortKey="Dickinson, M J" sort="Dickinson, M J" uniqKey="Dickinson M" first="M J" last="Dickinson">M J Dickinson</name><affiliation><nlm:affiliation>CSIRO, Division of Plant Industry, Canberra, ACT, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, R" sort="Zhang, R" uniqKey="Zhang R" first="R" last="Zhang">R. 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The dsRNAs are inherited in three genetic units: the L unit comprising a single 5.2 kbp dsRNA and contained within a 40-nm virus-like particle, and the A and B units each consisting of five dsRNAs (A1-A5, and B1-B5) ranging in size from 1.2 to 2.7 kbp. This paper reports the isolation of a cDNA library representing 10 of the 11 dsRNAs. By nucleic-acid hybridization techniques it has been shown that all ten sequences are unique showing no detectable cross-hybridization with any other dsRNA present in the rust. A near full-length sequence of 1932 bp of the B3 dsRNA is reported and contains several open reading frames, the largest of which comprises most of the molecule.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">8299159</PMID><DateCompleted><Year>1994</Year><Month>03</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0172-8083</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>5</Issue><PubDate><Year>1993</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Current genetics</Title><ISOAbbreviation>Curr Genet</ISOAbbreviation></Journal><ArticleTitle>Nucleotide sequence relationships of double-stranded RNAs in flax rust, Melampsora lini.</ArticleTitle><Pagination><MedlinePgn>428-32</MedlinePgn></Pagination><Abstract><AbstractText>Flax rust, Melampsora lini strain SP6, contains 11 double-stranded (ds) RNA molecules with a total length of about 25 kbp. The dsRNAs are inherited in three genetic units: the L unit comprising a single 5.2 kbp dsRNA and contained within a 40-nm virus-like particle, and the A and B units each consisting of five dsRNAs (A1-A5, and B1-B5) ranging in size from 1.2 to 2.7 kbp. This paper reports the isolation of a cDNA library representing 10 of the 11 dsRNAs. By nucleic-acid hybridization techniques it has been shown that all ten sequences are unique showing no detectable cross-hybridization with any other dsRNA present in the rust. 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