MelampsoraV2, PubMed, Corpus, bibRecord, 000183

[Specific molecular markers of the rust resistance gene M4 in flax].

Identifieur interne : 000183 ( PubMed/Corpus ); précédent : 000182; suivant : 000184

[Specific molecular markers of the rust resistance gene M4 in flax].

Auteurs : Tian-Yue Bo ; Hua-Zhi Ye ; Shi-Quan Wang ; Jian-Chun Yang ; Xiao-Bing Li ; Wen-Xue Zhai

Source :

Yi chuan xue bao = Acta genetica Sinica [ 0379-4172 ] ; 2002.

RBID : pubmed:12561479

English descriptors

KwdEn :
- Base Sequence (MeSH), Basidiomycota (growth & development), Crosses, Genetic (MeSH), DNA Restriction Enzymes (metabolism), DNA, Plant (genetics), DNA, Plant (metabolism), Flax (genetics), Flax (microbiology), Genes, Plant (genetics), Genetic Markers (MeSH), Immunity, Innate (genetics), Molecular Sequence Data (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Random Amplified Polymorphic DNA Technique (MeSH).
MESH :
- chemical , genetics : DNA, Plant.
- chemical , metabolism : DNA Restriction Enzymes, DNA, Plant.
- genetics : Flax, Genes, Plant, Immunity, Innate, Plant Diseases.
- growth & development : Basidiomycota.
- microbiology : Flax, Plant Diseases.
- Base Sequence, Crosses, Genetic, Genetic Markers, Molecular Sequence Data, Random Amplified Polymorphic DNA Technique.

Abstract

Flax (Linum usitatissimum L.) is an important fiber and oil-producing crop. Flax rust, caused by Melampsora lini Ehrenb. Lev., occurs worldwide and can cause severe losses in seed yield and fiber quality. In order to identify molecular markers linked to the flax rust resistant gene M4, RAPD analysis of NM4, a near-isogenic line containing the M4 gene, and the recurrent parent Bison was carried out with 540 decamer primers. The primer OPA18 could stably amplify a specific fragment, OPA18(432), in the NM4 line. The OPA18(432) marker was testified to be closely linked to the M4 gene with a genetic distance of 2.1 cM through the analysis of the F2 mapping population derived from a cross of Bison x NM4. Based on the sequence of OPA18(432), the specific PCR primers were designed, and a SCAR marker for the M4 gene was produced. Amplification of different resistant materials proved that the maker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.

PubMed: 12561479

Links to Exploration step

pubmed:12561479

Le document en format XML

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<author><name sortKey="Bo, Tian Yue" sort="Bo, Tian Yue" uniqKey="Bo T" first="Tian-Yue" last="Bo">Tian-Yue Bo</name>
<affiliation><nlm:affiliation>Cold Region Crop Research Institute, Shanxi Academy of Agricultural Sciences, Datong 037008, China.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Ye, Hua Zhi" sort="Ye, Hua Zhi" uniqKey="Ye H" first="Hua-Zhi" last="Ye">Hua-Zhi Ye</name>
</author>
<author><name sortKey="Wang, Shi Quan" sort="Wang, Shi Quan" uniqKey="Wang S" first="Shi-Quan" last="Wang">Shi-Quan Wang</name>
</author>
<author><name sortKey="Yang, Jian Chun" sort="Yang, Jian Chun" uniqKey="Yang J" first="Jian-Chun" last="Yang">Jian-Chun Yang</name>
</author>
<author><name sortKey="Li, Xiao Bing" sort="Li, Xiao Bing" uniqKey="Li X" first="Xiao-Bing" last="Li">Xiao-Bing Li</name>
</author>
<author><name sortKey="Zhai, Wen Xue" sort="Zhai, Wen Xue" uniqKey="Zhai W" first="Wen-Xue" last="Zhai">Wen-Xue Zhai</name>
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<affiliation><nlm:affiliation>Cold Region Crop Research Institute, Shanxi Academy of Agricultural Sciences, Datong 037008, China.</nlm:affiliation>
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<author><name sortKey="Ye, Hua Zhi" sort="Ye, Hua Zhi" uniqKey="Ye H" first="Hua-Zhi" last="Ye">Hua-Zhi Ye</name>
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<author><name sortKey="Wang, Shi Quan" sort="Wang, Shi Quan" uniqKey="Wang S" first="Shi-Quan" last="Wang">Shi-Quan Wang</name>
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<author><name sortKey="Yang, Jian Chun" sort="Yang, Jian Chun" uniqKey="Yang J" first="Jian-Chun" last="Yang">Jian-Chun Yang</name>
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<author><name sortKey="Li, Xiao Bing" sort="Li, Xiao Bing" uniqKey="Li X" first="Xiao-Bing" last="Li">Xiao-Bing Li</name>
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<author><name sortKey="Zhai, Wen Xue" sort="Zhai, Wen Xue" uniqKey="Zhai W" first="Wen-Xue" last="Zhai">Wen-Xue Zhai</name>
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<series><title level="j">Yi chuan xue bao = Acta genetica Sinica</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term>
<term>Basidiomycota (growth & development)</term>
<term>Crosses, Genetic (MeSH)</term>
<term>DNA Restriction Enzymes (metabolism)</term>
<term>DNA, Plant (genetics)</term>
<term>DNA, Plant (metabolism)</term>
<term>Flax (genetics)</term>
<term>Flax (microbiology)</term>
<term>Genes, Plant (genetics)</term>
<term>Genetic Markers (MeSH)</term>
<term>Immunity, Innate (genetics)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Random Amplified Polymorphic DNA Technique (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Plant</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA Restriction Enzymes</term>
<term>DNA, Plant</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flax</term>
<term>Genes, Plant</term>
<term>Immunity, Innate</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Flax</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Crosses, Genetic</term>
<term>Genetic Markers</term>
<term>Molecular Sequence Data</term>
<term>Random Amplified Polymorphic DNA Technique</term>
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<front><div type="abstract" xml:lang="en">Flax (Linum usitatissimum L.) is an important fiber and oil-producing crop. Flax rust, caused by Melampsora lini Ehrenb. Lev., occurs worldwide and can cause severe losses in seed yield and fiber quality. In order to identify molecular markers linked to the flax rust resistant gene M4, RAPD analysis of NM4, a near-isogenic line containing the M4 gene, and the recurrent parent Bison was carried out with 540 decamer primers. The primer OPA18 could stably amplify a specific fragment, OPA18(432), in the NM4 line. The OPA18(432) marker was testified to be closely linked to the M4 gene with a genetic distance of 2.1 cM through the analysis of the F2 mapping population derived from a cross of Bison x NM4. Based on the sequence of OPA18(432), the specific PCR primers were designed, and a SCAR marker for the M4 gene was produced. Amplification of different resistant materials proved that the maker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.</div>
</front>
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<DateCompleted><Year>2003</Year>
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<Day>23</Day>
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<DateRevised><Year>2008</Year>
<Month>11</Month>
<Day>21</Day>
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<Article PubModel="Print"><Journal><ISSN IssnType="Print">0379-4172</ISSN>
<JournalIssue CitedMedium="Print"><Volume>29</Volume>
<Issue>10</Issue>
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<Month>Oct</Month>
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<Title>Yi chuan xue bao = Acta genetica Sinica</Title>
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<ArticleTitle>[Specific molecular markers of the rust resistance gene M4 in flax].</ArticleTitle>
<Pagination><MedlinePgn>922-7</MedlinePgn>
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<Abstract><AbstractText>Flax (Linum usitatissimum L.) is an important fiber and oil-producing crop. Flax rust, caused by Melampsora lini Ehrenb. Lev., occurs worldwide and can cause severe losses in seed yield and fiber quality. In order to identify molecular markers linked to the flax rust resistant gene M4, RAPD analysis of NM4, a near-isogenic line containing the M4 gene, and the recurrent parent Bison was carried out with 540 decamer primers. The primer OPA18 could stably amplify a specific fragment, OPA18(432), in the NM4 line. The OPA18(432) marker was testified to be closely linked to the M4 gene with a genetic distance of 2.1 cM through the analysis of the F2 mapping population derived from a cross of Bison x NM4. Based on the sequence of OPA18(432), the specific PCR primers were designed, and a SCAR marker for the M4 gene was produced. Amplification of different resistant materials proved that the maker is specific for the M4 gene. This marker has been used successfully in marker-assisted selection in the flax breeding program.</AbstractText>
</Abstract>
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<ForeName>Tian-Yue</ForeName>
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<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
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<QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName>
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<MeshHeading><DescriptorName UI="D019105" MajorTopicYN="N">Random Amplified Polymorphic DNA Technique</DescriptorName>
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[Specific molecular markers of the rust resistance gene M4 in flax].

[Specific molecular markers of the rust resistance gene M4 in flax].

Source :

English descriptors

Abstract

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki