A rust-inducible gene from flax (fis1) is involved in proline catabolism.
Identifieur interne : 000163 ( PubMed/Corpus ); précédent : 000162; suivant : 000164A rust-inducible gene from flax (fis1) is involved in proline catabolism.
Auteurs : Heidi J. Mitchell ; Michael A. Ayliffe ; Khalid Y. Rashid ; Anthony J. PryorSource :
- Planta [ 0032-0935 ] ; 2006.
English descriptors
- KwdEn :
- 1-Pyrroline-5-Carboxylate Dehydrogenase (biosynthesis), 1-Pyrroline-5-Carboxylate Dehydrogenase (genetics), 1-Pyrroline-5-Carboxylate Dehydrogenase (physiology), Basidiomycota (pathogenicity), DNA Mutational Analysis (MeSH), Flax (enzymology), Flax (genetics), Flax (microbiology), Gene Expression Regulation, Plant (MeSH), Genes, Reporter (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (biosynthesis), Plant Proteins (genetics), Plant Proteins (physiology), Plants, Genetically Modified (anatomy & histology), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (metabolism), Proline (metabolism), Proline (toxicity), Promoter Regions, Genetic (MeSH), Transcriptional Activation (MeSH).
- MESH :
- chemical , biosynthesis : 1-Pyrroline-5-Carboxylate Dehydrogenase, Plant Proteins.
- chemical , genetics : 1-Pyrroline-5-Carboxylate Dehydrogenase, Plant Proteins.
- chemical , metabolism : Proline.
- chemical , physiology : 1-Pyrroline-5-Carboxylate Dehydrogenase, Plant Proteins.
- anatomy & histology : Plants, Genetically Modified.
- drug effects : Plants, Genetically Modified.
- enzymology : Flax.
- genetics : Flax, Plant Diseases.
- metabolism : Plants, Genetically Modified.
- microbiology : Flax, Plant Diseases.
- pathogenicity : Basidiomycota.
- chemical , toxicity : Proline.
- DNA Mutational Analysis, Gene Expression Regulation, Plant, Genes, Reporter, Promoter Regions, Genetic, Transcriptional Activation.
Abstract
A gene fis1 from flax (Linum usitatissimum), which is induced in mesophyll cells at the site of rust (Melampsora lini) infection, is also expressed in vascular tissue, particularly in floral structures of healthy plants. This paper reports that the promoter controlling this expression is contained within 282 bp 5' to the coding region and that fis1 gene induction is specifically by the rust pathogen and not by other fungal pathogens or by wounding. The fis1 gene has 73% homology with an Arabidopsis gene which encodes delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDH) which is a part of the proline degradation pathway. Transgenic flax plants that either over-express fis1 or show reduced fis1 expression due to RNA-mediated gene silencing have an unaltered morphology. However, plants with reduced fis1 expression have markedly increased sensitivity to exogenous proline and show alteration in epidermal cell morphology, callose deposition and the production of hydrogen peroxide during proline-induced death. These lines, which show a biologically significant level of fis1 suppression, have an unaltered reaction to either virulent or avirulent rust infections, as do fis1 over-expression lines. These data indicate that the fis1 gene plays a role in proline metabolism and most likely encodes for a P5CDH enzyme. However, the precise role of fis1 and P5C catabolism in the development of rust disease remains unclear.
DOI: 10.1007/s00425-005-0079-x
PubMed: 16079997
Links to Exploration step
pubmed:16079997Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A rust-inducible gene from flax (fis1) is involved in proline catabolism.</title><author><name sortKey="Mitchell, Heidi J" sort="Mitchell, Heidi J" uniqKey="Mitchell H" first="Heidi J" last="Mitchell">Heidi J. Mitchell</name><affiliation><nlm:affiliation>CSIRO Division of Plant Industry, Box 1600, Canberra, ACT 2601, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Ayliffe, Michael A" sort="Ayliffe, Michael A" uniqKey="Ayliffe M" first="Michael A" last="Ayliffe">Michael A. Ayliffe</name></author><author><name sortKey="Rashid, Khalid Y" sort="Rashid, Khalid Y" uniqKey="Rashid K" first="Khalid Y" last="Rashid">Khalid Y. Rashid</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. Pryor</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16079997</idno><idno type="pmid">16079997</idno><idno type="doi">10.1007/s00425-005-0079-x</idno><idno type="wicri:Area/PubMed/Corpus">000163</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000163</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A rust-inducible gene from flax (fis1) is involved in proline catabolism.</title><author><name sortKey="Mitchell, Heidi J" sort="Mitchell, Heidi J" uniqKey="Mitchell H" first="Heidi J" last="Mitchell">Heidi J. Mitchell</name><affiliation><nlm:affiliation>CSIRO Division of Plant Industry, Box 1600, Canberra, ACT 2601, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Ayliffe, Michael A" sort="Ayliffe, Michael A" uniqKey="Ayliffe M" first="Michael A" last="Ayliffe">Michael A. Ayliffe</name></author><author><name sortKey="Rashid, Khalid Y" sort="Rashid, Khalid Y" uniqKey="Rashid K" first="Khalid Y" last="Rashid">Khalid Y. Rashid</name></author><author><name sortKey="Pryor, Anthony J" sort="Pryor, Anthony J" uniqKey="Pryor A" first="Anthony J" last="Pryor">Anthony J. Pryor</name></author></analytic><series><title level="j">Planta</title><idno type="ISSN">0032-0935</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>1-Pyrroline-5-Carboxylate Dehydrogenase (biosynthesis)</term><term>1-Pyrroline-5-Carboxylate Dehydrogenase (genetics)</term><term>1-Pyrroline-5-Carboxylate Dehydrogenase (physiology)</term><term>Basidiomycota (pathogenicity)</term><term>DNA Mutational Analysis (MeSH)</term><term>Flax (enzymology)</term><term>Flax (genetics)</term><term>Flax (microbiology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Reporter (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (biosynthesis)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (physiology)</term><term>Plants, Genetically Modified (anatomy & histology)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (metabolism)</term><term>Proline (metabolism)</term><term>Proline (toxicity)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Transcriptional Activation (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>1-Pyrroline-5-Carboxylate Dehydrogenase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>1-Pyrroline-5-Carboxylate Dehydrogenase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Proline</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>1-Pyrroline-5-Carboxylate Dehydrogenase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Flax</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Flax</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Flax</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Proline</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Mutational Analysis</term><term>Gene Expression Regulation, Plant</term><term>Genes, Reporter</term><term>Promoter Regions, Genetic</term><term>Transcriptional Activation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A gene fis1 from flax (Linum usitatissimum), which is induced in mesophyll cells at the site of rust (Melampsora lini) infection, is also expressed in vascular tissue, particularly in floral structures of healthy plants. This paper reports that the promoter controlling this expression is contained within 282 bp 5' to the coding region and that fis1 gene induction is specifically by the rust pathogen and not by other fungal pathogens or by wounding. The fis1 gene has 73% homology with an Arabidopsis gene which encodes delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDH) which is a part of the proline degradation pathway. Transgenic flax plants that either over-express fis1 or show reduced fis1 expression due to RNA-mediated gene silencing have an unaltered morphology. However, plants with reduced fis1 expression have markedly increased sensitivity to exogenous proline and show alteration in epidermal cell morphology, callose deposition and the production of hydrogen peroxide during proline-induced death. These lines, which show a biologically significant level of fis1 suppression, have an unaltered reaction to either virulent or avirulent rust infections, as do fis1 over-expression lines. These data indicate that the fis1 gene plays a role in proline metabolism and most likely encodes for a P5CDH enzyme. However, the precise role of fis1 and P5C catabolism in the development of rust disease remains unclear.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16079997</PMID><DateCompleted><Year>2006</Year><Month>04</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0935</ISSN><JournalIssue CitedMedium="Print"><Volume>223</Volume><Issue>2</Issue><PubDate><Year>2006</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>A rust-inducible gene from flax (fis1) is involved in proline catabolism.</ArticleTitle><Pagination><MedlinePgn>213-22</MedlinePgn></Pagination><Abstract><AbstractText>A gene fis1 from flax (Linum usitatissimum), which is induced in mesophyll cells at the site of rust (Melampsora lini) infection, is also expressed in vascular tissue, particularly in floral structures of healthy plants. This paper reports that the promoter controlling this expression is contained within 282 bp 5' to the coding region and that fis1 gene induction is specifically by the rust pathogen and not by other fungal pathogens or by wounding. The fis1 gene has 73% homology with an Arabidopsis gene which encodes delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDH) which is a part of the proline degradation pathway. Transgenic flax plants that either over-express fis1 or show reduced fis1 expression due to RNA-mediated gene silencing have an unaltered morphology. However, plants with reduced fis1 expression have markedly increased sensitivity to exogenous proline and show alteration in epidermal cell morphology, callose deposition and the production of hydrogen peroxide during proline-induced death. These lines, which show a biologically significant level of fis1 suppression, have an unaltered reaction to either virulent or avirulent rust infections, as do fis1 over-expression lines. These data indicate that the fis1 gene plays a role in proline metabolism and most likely encodes for a P5CDH enzyme. However, the precise role of fis1 and P5C catabolism in the development of rust disease remains unclear.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mitchell</LastName><ForeName>Heidi J</ForeName><Initials>HJ</Initials><AffiliationInfo><Affiliation>CSIRO Division of Plant Industry, Box 1600, Canberra, ACT 2601, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ayliffe</LastName><ForeName>Michael A</ForeName><Initials>MA</Initials></Author><Author ValidYN="Y"><LastName>Rashid</LastName><ForeName>Khalid Y</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Pryor</LastName><ForeName>Anthony J</ForeName><Initials>AJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2005</Year><Month>08</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Planta</MedlineTA><NlmUniqueID>1250576</NlmUniqueID><ISSNLinking>0032-0935</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>9DLQ4CIU6V</RegistryNumber><NameOfSubstance UI="D011392">Proline</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.2.1.88</RegistryNumber><NameOfSubstance UI="D050842">1-Pyrroline-5-Carboxylate Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D050842" MajorTopicYN="N">1-Pyrroline-5-Carboxylate Dehydrogenase</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004252" MajorTopicYN="N">DNA Mutational Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019597" MajorTopicYN="N">Flax</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017930" MajorTopicYN="N">Genes, Reporter</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011392" MajorTopicYN="N">Proline</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015533" MajorTopicYN="N">Transcriptional Activation</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2005</Year><Month>06</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2005</Year><Month>07</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>8</Month><Day>5</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>4</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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