Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.
Identifieur interne : 000126 ( PubMed/Corpus ); précédent : 000125; suivant : 000127Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.
Auteurs : Marlien M. Van Der Merwe ; Mark W. Kinnear ; Luke G. Barrett ; Peter N. Dodds ; Lars Ericson ; Peter H. Thrall ; Jeremy J. BurdonSource :
- Proceedings. Biological sciences [ 0962-8452 ] ; 2009.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Fungal Proteins (chemistry), Fungal Proteins (genetics), Fungal Proteins (metabolism), Fungi (classification), Fungi (genetics), Fungi (pathogenicity), Gene Expression Regulation, Fungal (physiology), Genetic Variation (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Diseases (microbiology), Selection, Genetic (MeSH), Virulence (MeSH).
- MESH :
- chemical , chemistry : Fungal Proteins.
- chemical , genetics : Fungal Proteins.
- chemical , metabolism : Fungal Proteins.
- classification : Fungi.
- genetics : Fungi.
- microbiology : Plant Diseases.
- pathogenicity : Fungi.
- physiology : Gene Expression Regulation, Fungal.
- Amino Acid Sequence, Genetic Variation, Molecular Sequence Data, Phylogeny, Selection, Genetic, Virulence.
Abstract
Pathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, AvrP4, in the plant pathogen Melampsora lini, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the Melampsora species. We amplified AvrP4 homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the AvrP4 sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus non-synonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.
DOI: 10.1098/rspb.2009.0328
PubMed: 19457888
PubMed Central: PMC2817207
Links to Exploration step
pubmed:19457888Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.</title><author><name sortKey="Van Der Merwe, Marlien M" sort="Van Der Merwe, Marlien M" uniqKey="Van Der Merwe M" first="Marlien M" last="Van Der Merwe">Marlien M. Van Der Merwe</name><affiliation><nlm:affiliation>CSIRO-Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia. marlien.vandermerwe@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Kinnear, Mark W" sort="Kinnear, Mark W" uniqKey="Kinnear M" first="Mark W" last="Kinnear">Mark W. Kinnear</name></author><author><name sortKey="Barrett, Luke G" sort="Barrett, Luke G" uniqKey="Barrett L" first="Luke G" last="Barrett">Luke G. Barrett</name></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N" last="Dodds">Peter N. Dodds</name></author><author><name sortKey="Ericson, Lars" sort="Ericson, Lars" uniqKey="Ericson L" first="Lars" last="Ericson">Lars Ericson</name></author><author><name sortKey="Thrall, Peter H" sort="Thrall, Peter H" uniqKey="Thrall P" first="Peter H" last="Thrall">Peter H. Thrall</name></author><author><name sortKey="Burdon, Jeremy J" sort="Burdon, Jeremy J" uniqKey="Burdon J" first="Jeremy J" last="Burdon">Jeremy J. Burdon</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19457888</idno><idno type="pmid">19457888</idno><idno type="doi">10.1098/rspb.2009.0328</idno><idno type="pmc">PMC2817207</idno><idno type="wicri:Area/PubMed/Corpus">000126</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000126</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.</title><author><name sortKey="Van Der Merwe, Marlien M" sort="Van Der Merwe, Marlien M" uniqKey="Van Der Merwe M" first="Marlien M" last="Van Der Merwe">Marlien M. Van Der Merwe</name><affiliation><nlm:affiliation>CSIRO-Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia. marlien.vandermerwe@gmail.com</nlm:affiliation></affiliation></author><author><name sortKey="Kinnear, Mark W" sort="Kinnear, Mark W" uniqKey="Kinnear M" first="Mark W" last="Kinnear">Mark W. Kinnear</name></author><author><name sortKey="Barrett, Luke G" sort="Barrett, Luke G" uniqKey="Barrett L" first="Luke G" last="Barrett">Luke G. Barrett</name></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N" last="Dodds">Peter N. Dodds</name></author><author><name sortKey="Ericson, Lars" sort="Ericson, Lars" uniqKey="Ericson L" first="Lars" last="Ericson">Lars Ericson</name></author><author><name sortKey="Thrall, Peter H" sort="Thrall, Peter H" uniqKey="Thrall P" first="Peter H" last="Thrall">Peter H. Thrall</name></author><author><name sortKey="Burdon, Jeremy J" sort="Burdon, Jeremy J" uniqKey="Burdon J" first="Jeremy J" last="Burdon">Jeremy J. Burdon</name></author></analytic><series><title level="j">Proceedings. Biological sciences</title><idno type="ISSN">0962-8452</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Fungal Proteins (chemistry)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Fungi (classification)</term><term>Fungi (genetics)</term><term>Fungi (pathogenicity)</term><term>Gene Expression Regulation, Fungal (physiology)</term><term>Genetic Variation (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phylogeny (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Selection, Genetic (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Genetic Variation</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Selection, Genetic</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, AvrP4, in the plant pathogen Melampsora lini, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the Melampsora species. We amplified AvrP4 homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the AvrP4 sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus non-synonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19457888</PMID><DateCompleted><Year>2009</Year><Month>09</Month><Day>17</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0962-8452</ISSN><JournalIssue CitedMedium="Print"><Volume>276</Volume><Issue>1669</Issue><PubDate><Year>2009</Year><Month>Aug</Month><Day>22</Day></PubDate></JournalIssue><Title>Proceedings. Biological sciences</Title><ISOAbbreviation>Proc Biol Sci</ISOAbbreviation></Journal><ArticleTitle>Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.</ArticleTitle><Pagination><MedlinePgn>2913-22</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1098/rspb.2009.0328</ELocationID><Abstract><AbstractText>Pathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, AvrP4, in the plant pathogen Melampsora lini, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the Melampsora species. We amplified AvrP4 homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the AvrP4 sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus non-synonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Van der Merwe</LastName><ForeName>Marlien M</ForeName><Initials>MM</Initials><AffiliationInfo><Affiliation>CSIRO-Plant Industry, GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia. marlien.vandermerwe@gmail.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kinnear</LastName><ForeName>Mark W</ForeName><Initials>MW</Initials></Author><Author ValidYN="Y"><LastName>Barrett</LastName><ForeName>Luke G</ForeName><Initials>LG</Initials></Author><Author ValidYN="Y"><LastName>Dodds</LastName><ForeName>Peter N</ForeName><Initials>PN</Initials></Author><Author ValidYN="Y"><LastName>Ericson</LastName><ForeName>Lars</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Thrall</LastName><ForeName>Peter H</ForeName><Initials>PH</Initials></Author><Author ValidYN="Y"><LastName>Burdon</LastName><ForeName>Jeremy J</ForeName><Initials>JJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>05</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Proc Biol Sci</MedlineTA><NlmUniqueID>101245157</NlmUniqueID><ISSNLinking>0962-8452</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015966" MajorTopicYN="N">Gene Expression Regulation, Fungal</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014644" MajorTopicYN="N">Genetic Variation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012641" MajorTopicYN="Y">Selection, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>5</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>5</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>9</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19457888</ArticleId><ArticleId IdType="pii">rspb.2009.0328</ArticleId><ArticleId IdType="doi">10.1098/rspb.2009.0328</ArticleId><ArticleId IdType="pmc">PMC2817207</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Biol Sci. 2000 Nov 7;267(1458):2183-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11413631</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 2000 May;155(1):431-49</Citation><ArticleIdList><ArticleId IdType="pubmed">10790415</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 1998 Jul;29(1):165-77</Citation><ArticleIdList><ArticleId IdType="pubmed">9701811</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2006 May;60(4):951-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16677306</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2005 Nov;18(11):1148-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16353550</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Sep 1;313(5791):1261-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16946064</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Evol. 1992 Feb;34(2):126-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1556748</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2007 Jul;8(4):349-64</Citation><ArticleIdList><ArticleId IdType="pubmed">20507505</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1998 Mar;148(3):929-36</Citation><ArticleIdList><ArticleId IdType="pubmed">9539414</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2004;42:385-414</Citation><ArticleIdList><ArticleId IdType="pubmed">15283671</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2005 Jan 15;21(2):260-2</Citation><ArticleIdList><ArticleId IdType="pubmed">15377507</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2004 Dec 10;306(5703):1957-60</Citation><ArticleIdList><ArticleId IdType="pubmed">15591208</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2004 Sep;53(5):1373-83</Citation><ArticleIdList><ArticleId IdType="pubmed">15387816</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Jan;18(1):243-56</Citation><ArticleIdList><ArticleId IdType="pubmed">16326930</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2003 Aug 12;19(12):1572-4</Citation><ArticleIdList><ArticleId IdType="pubmed">12912839</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2005 Mar;22(3):659-72</Citation><ArticleIdList><ArticleId IdType="pubmed">15548752</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Genet. 2001 Jul;2(7):516-27</Citation><ArticleIdList><ArticleId IdType="pubmed">11433358</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1997 Mar 10;404(2-3):153-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9119054</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Sci. 1994 Oct;3(10):1833-9</Citation><ArticleIdList><ArticleId IdType="pubmed">7849598</ArticleId></ArticleIdList></Reference><Reference><Citation>Mycol Res. 2007 Feb;111(Pt 2):163-75</Citation><ArticleIdList><ArticleId IdType="pubmed">17324755</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Genet. 2007 Dec;39(12):1461-8</Citation><ArticleIdList><ArticleId IdType="pubmed">17987029</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2001 Jun 14;411(6839):826-33</Citation><ArticleIdList><ArticleId IdType="pubmed">11459065</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2005 Apr;22(4):1107-18</Citation><ArticleIdList><ArticleId IdType="pubmed">15689528</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2007 Aug;19(8):2349-69</Citation><ArticleIdList><ArticleId IdType="pubmed">17675403</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2004 Mar;16(3):755-68</Citation><ArticleIdList><ArticleId IdType="pubmed">14973158</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2005 Sep;18(9):1011-21</Citation><ArticleIdList><ArticleId IdType="pubmed">16167771</ArticleId></ArticleIdList></Reference><Reference><Citation>Evolution. 2007 Aug;61(8):1812-22</Citation><ArticleIdList><ArticleId IdType="pubmed">17683425</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2006 Jan 13;311(5758):222-6</Citation><ArticleIdList><ArticleId IdType="pubmed">16373536</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbiology. 2001 May;147(Pt 5):1171-82</Citation><ArticleIdList><ArticleId IdType="pubmed">11320120</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2002 Jan;15(1):6-16</Citation><ArticleIdList><ArticleId IdType="pubmed">11843304</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Jun 6;103(23):8888-93</Citation><ArticleIdList><ArticleId IdType="pubmed">16731621</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2007 Oct;20(10):1271-83</Citation><ArticleIdList><ArticleId IdType="pubmed">17918629</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 1998;14(9):817-8</Citation><ArticleIdList><ArticleId IdType="pubmed">9918953</ArticleId></ArticleIdList></Reference><Reference><Citation>J Theor Biol. 1999 Nov 7;201(1):1-12</Citation><ArticleIdList><ArticleId IdType="pubmed">10534431</ArticleId></ArticleIdList></Reference><Reference><Citation>EMBO J. 2000 Jul 3;19(13):3204-14</Citation><ArticleIdList><ArticleId IdType="pubmed">10880434</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2007;45:289-306</Citation><ArticleIdList><ArticleId IdType="pubmed">17430087</ArticleId></ArticleIdList></Reference><Reference><Citation>Comput Appl Biosci. 1997 Oct;13(5):555-6</Citation><ArticleIdList><ArticleId IdType="pubmed">9367129</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Biol Sci. 2007 Mar 22;274(1611):809-17</Citation><ArticleIdList><ArticleId IdType="pubmed">17251091</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4874-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18344324</ArticleId></ArticleIdList></Reference><Reference><Citation>Brief Bioinform. 2004 Jun;5(2):150-63</Citation><ArticleIdList><ArticleId IdType="pubmed">15260895</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2001 Aug;18(8):1585-92</Citation><ArticleIdList><ArticleId IdType="pubmed">11470850</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2001 Aug;17(8):754-5</Citation><ArticleIdList><ArticleId IdType="pubmed">11524383</ArticleId></ArticleIdList></Reference><Reference><Citation>Syst Biol. 2008 Oct;57(5):758-71</Citation><ArticleIdList><ArticleId IdType="pubmed">18853362</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioinformatics. 2000 Jun;16(6):562-3</Citation><ArticleIdList><ArticleId IdType="pubmed">10980155</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2005 Jun;17(6):1839-50</Citation><ArticleIdList><ArticleId IdType="pubmed">15894715</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1999 Dec 20;265(2):218-25</Citation><ArticleIdList><ArticleId IdType="pubmed">10600594</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000126 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000126 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:19457888
|texte= Positive selection in AvrP4 avirulence gene homologues across the genus Melampsora.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:19457888" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a MelampsoraV2
| This area was generated with Dilib version V0.6.38. |  |