Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense.
Identifieur interne : 000123 ( PubMed/Corpus ); précédent : 000122; suivant : 000124Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense.
Auteurs : Valérie Levée ; Ian Major ; Caroline Levasseur ; Laurence Tremblay ; John Mackay ; Armand SéguinSource :
- The New phytologist [ 1469-8137 ] ; 2009.
English descriptors
- KwdEn :
- Basidiomycota (drug effects), Basidiomycota (growth & development), Cell Wall (drug effects), Cell Wall (genetics), Chitosan (pharmacology), DNA, Complementary (isolation & purification), Gene Expression Profiling (MeSH), Gene Expression Regulation, Plant (drug effects), Hydrogen Peroxide (metabolism), Lignin (metabolism), Oligonucleotide Array Sequence Analysis (MeSH), Peroxidase (metabolism), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Leaves (drug effects), Plant Leaves (genetics), Plant Leaves (microbiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (MeSH), Populus (drug effects), Populus (enzymology), Populus (genetics), Populus (immunology), RNA, Messenger (genetics), RNA, Messenger (metabolism), Salicylic Acid (pharmacology), Stress, Physiological (drug effects), Stress, Physiological (genetics), Wood (drug effects), Wood (genetics).
- MESH :
- chemical , genetics : Plant Proteins, RNA, Messenger.
- chemical , isolation & purification : DNA, Complementary.
- chemical , metabolism : Hydrogen Peroxide, Lignin, Peroxidase, Plant Proteins, RNA, Messenger.
- chemical , pharmacology : Chitosan, Salicylic Acid.
- drug effects : Basidiomycota, Cell Wall, Gene Expression Regulation, Plant, Plant Leaves, Populus, Stress, Physiological, Wood.
- enzymology : Populus.
- genetics : Cell Wall, Plant Diseases, Plant Leaves, Populus, Stress, Physiological, Wood.
- growth & development : Basidiomycota.
- immunology : Populus.
- microbiology : Plant Diseases, Plant Leaves.
- Gene Expression Profiling, Oligonucleotide Array Sequence Analysis, Plants, Genetically Modified.
Abstract
WRKY transcription factors are key regulators that activate and fine-tune stress responses, including defense responses against pathogens. We isolated a poplar (Populus tremulaxPopulus alba) cDNA sequence, PtWRKY23, that encodes the ortholog of Arabidopsis WRKY23 and present the functional analysis of WRKY23, with emphasis on its potential role in resistance to rust infection. To investigate the function of PtWRKY23, we examined PtWRKY23 expression after stress treatments by qRT-PCR and generated PtWRKY23-misexpressing plants. Transgenic plants were assessed for resistance to Melampsora rust and were analyzed using the poplar Affymetrix GeneChip and histological techniques to study the consequences of PtWRKY23 misexpression. PtWRKY23 is rapidly induced by Melampsora infection and elicitor treatments and poplars overexpressing and underexpressing PtWRKY23 were both more susceptible to Melampsora infection than wild type. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also suggests that PtWRKY23 affects redox homeostasis and cell wall-related metabolism, which was confirmed by analyses that showed that PtWRKY23-misexpressing plants have altered peroxidase activity, apparent H(2)O(2) accumulation and lignin deposition. Our results show that PtWRKY23 affects resistance to Melampsora infection and that this may be caused by deregulation of genes that disrupt redox homeostasis and cell wall metabolism.
DOI: 10.1111/j.1469-8137.2009.02955.x
PubMed: 19674332
Links to Exploration step
pubmed:19674332Le document en format XML
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uniqKey="Mackay J" first="John" last="Mackay">John Mackay</name></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19674332</idno><idno type="pmid">19674332</idno><idno type="doi">10.1111/j.1469-8137.2009.02955.x</idno><idno type="wicri:Area/PubMed/Corpus">000123</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000123</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense.</title><author><name sortKey="Levee, Valerie" sort="Levee, Valerie" uniqKey="Levee V" first="Valérie" last="Levée">Valérie Levée</name><affiliation><nlm:affiliation>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Stn Sainte-Foy, Québec, Quebec, Canada G1V 4C7.</nlm:affiliation></affiliation></author><author><name sortKey="Major, Ian" sort="Major, Ian" uniqKey="Major I" first="Ian" last="Major">Ian Major</name></author><author><name sortKey="Levasseur, Caroline" sort="Levasseur, Caroline" uniqKey="Levasseur C" first="Caroline" last="Levasseur">Caroline Levasseur</name></author><author><name sortKey="Tremblay, Laurence" sort="Tremblay, Laurence" uniqKey="Tremblay L" first="Laurence" last="Tremblay">Laurence Tremblay</name></author><author><name sortKey="Mackay, John" sort="Mackay, John" uniqKey="Mackay J" first="John" last="Mackay">John Mackay</name></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (drug effects)</term><term>Basidiomycota (growth & development)</term><term>Cell Wall (drug effects)</term><term>Cell Wall (genetics)</term><term>Chitosan (pharmacology)</term><term>DNA, Complementary (isolation & purification)</term><term>Gene Expression Profiling (MeSH)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Hydrogen Peroxide (metabolism)</term><term>Lignin (metabolism)</term><term>Oligonucleotide Array Sequence Analysis (MeSH)</term><term>Peroxidase (metabolism)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (drug effects)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (immunology)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>Salicylic Acid (pharmacology)</term><term>Stress, Physiological (drug effects)</term><term>Stress, Physiological (genetics)</term><term>Wood (drug effects)</term><term>Wood (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hydrogen Peroxide</term><term>Lignin</term><term>Peroxidase</term><term>Plant Proteins</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chitosan</term><term>Salicylic Acid</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Basidiomycota</term><term>Cell Wall</term><term>Gene Expression Regulation, Plant</term><term>Plant Leaves</term><term>Populus</term><term>Stress, Physiological</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Wall</term><term>Plant Diseases</term><term>Plant Leaves</term><term>Populus</term><term>Stress, Physiological</term><term>Wood</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Oligonucleotide Array Sequence Analysis</term><term>Plants, Genetically Modified</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">WRKY transcription factors are key regulators that activate and fine-tune stress responses, including defense responses against pathogens. We isolated a poplar (Populus tremulaxPopulus alba) cDNA sequence, PtWRKY23, that encodes the ortholog of Arabidopsis WRKY23 and present the functional analysis of WRKY23, with emphasis on its potential role in resistance to rust infection. To investigate the function of PtWRKY23, we examined PtWRKY23 expression after stress treatments by qRT-PCR and generated PtWRKY23-misexpressing plants. Transgenic plants were assessed for resistance to Melampsora rust and were analyzed using the poplar Affymetrix GeneChip and histological techniques to study the consequences of PtWRKY23 misexpression. PtWRKY23 is rapidly induced by Melampsora infection and elicitor treatments and poplars overexpressing and underexpressing PtWRKY23 were both more susceptible to Melampsora infection than wild type. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also suggests that PtWRKY23 affects redox homeostasis and cell wall-related metabolism, which was confirmed by analyses that showed that PtWRKY23-misexpressing plants have altered peroxidase activity, apparent H(2)O(2) accumulation and lignin deposition. Our results show that PtWRKY23 affects resistance to Melampsora infection and that this may be caused by deregulation of genes that disrupt redox homeostasis and cell wall metabolism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19674332</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>184</Volume><Issue>1</Issue><PubDate><Year>2009</Year></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>Expression profiling and functional analysis of Populus WRKY23 reveals a regulatory role in defense.</ArticleTitle><Pagination><MedlinePgn>48-70</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1469-8137.2009.02955.x</ELocationID><Abstract><AbstractText>WRKY transcription factors are key regulators that activate and fine-tune stress responses, including defense responses against pathogens. We isolated a poplar (Populus tremulaxPopulus alba) cDNA sequence, PtWRKY23, that encodes the ortholog of Arabidopsis WRKY23 and present the functional analysis of WRKY23, with emphasis on its potential role in resistance to rust infection. To investigate the function of PtWRKY23, we examined PtWRKY23 expression after stress treatments by qRT-PCR and generated PtWRKY23-misexpressing plants. Transgenic plants were assessed for resistance to Melampsora rust and were analyzed using the poplar Affymetrix GeneChip and histological techniques to study the consequences of PtWRKY23 misexpression. PtWRKY23 is rapidly induced by Melampsora infection and elicitor treatments and poplars overexpressing and underexpressing PtWRKY23 were both more susceptible to Melampsora infection than wild type. Transcriptome analysis of PtWRKY23 overexpressors revealed a significant overlap with the Melampsora-infection response. Transcriptome analysis also suggests that PtWRKY23 affects redox homeostasis and cell wall-related metabolism, which was confirmed by analyses that showed that PtWRKY23-misexpressing plants have altered peroxidase activity, apparent H(2)O(2) accumulation and lignin deposition. Our results show that PtWRKY23 affects resistance to Melampsora infection and that this may be caused by deregulation of genes that disrupt redox homeostasis and cell wall metabolism.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Levée</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Natural Resources Canada, Canadian Forest Service, Laurentian Forestry Centre, Stn Sainte-Foy, Québec, Quebec, Canada G1V 4C7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Major</LastName><ForeName>Ian</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Levasseur</LastName><ForeName>Caroline</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Tremblay</LastName><ForeName>Laurence</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>MacKay</LastName><ForeName>John</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Séguin</LastName><ForeName>Armand</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>07</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>9012-76-4</RegistryNumber><NameOfSubstance UI="D048271">Chitosan</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.7</RegistryNumber><NameOfSubstance UI="D009195">Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D048271" MajorTopicYN="N">Chitosan</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="Y">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009195" MajorTopicYN="N">Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug 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Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate 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