Melampsora larici-populina transcript profiling during germination and timecourse infection of poplar leaves reveals dynamic expression patterns associated with virulence and biotrophy.
Identifieur interne : 000104 ( PubMed/Corpus ); précédent : 000103; suivant : 000105Melampsora larici-populina transcript profiling during germination and timecourse infection of poplar leaves reveals dynamic expression patterns associated with virulence and biotrophy.
Auteurs : Sébastien Duplessis ; Stéphane Hacquard ; Christine Delaruelle ; Emilie Tisserant ; Pascal Frey ; Francis Martin ; Annegret KohlerSource :
- Molecular plant-microbe interactions : MPMI [ 0894-0282 ] ; 2011.
English descriptors
- KwdEn :
- Basidiomycota (enzymology), Basidiomycota (genetics), Basidiomycota (pathogenicity), Basidiomycota (physiology), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation, Fungal (MeSH), Oligonucleotide Array Sequence Analysis (MeSH), Plant Diseases (microbiology), Plant Leaves (microbiology), Populus (microbiology), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Spores, Fungal (physiology).
- MESH :
- chemical , genetics : Fungal Proteins.
- enzymology : Basidiomycota.
- genetics : Basidiomycota.
- chemical , metabolism : Fungal Proteins.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- pathogenicity : Basidiomycota.
- physiology : Basidiomycota, Spores, Fungal.
- Gene Expression Profiling, Gene Expression Regulation, Fungal, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Melampsora larici-populina is responsible for poplar leaf rust disease and causes severe epidemics in poplar plantations in Europe. The poplar rust genome has been recently sequenced and, in order to find the genetic determinants associated with its biotrophic lifestyle, we generated a whole-genome custom oligoarray and analyzed transcript profiles of M. larici-populina during the infection timecourse in poplar leaves. Different stages were investigated during the asexual development of the rust fungus, including resting and germinating urediniospores and seven in planta stages in the telial host. In total, 76% of the transcripts were detected during leaf infection as well as in urediniospores, whereas 20% were only detected in planta, including several transporters and many small secreted proteins (SSP). We focused our analysis on gene categories known to be related to plant colonization and biotrophic growth in rust pathogens, such as SSP, carbohydrate active enzymes (CAZymes), transporters, lipases, and proteases. Distinct sets of SSP transcripts were expressed all along the infection process, suggesting highly dynamic expression of candidate rust effectors. In contrast, transcripts encoding transporters and proteases were mostly expressed after 48 h postinoculation, when numerous haustoria are already formed in the leaf mesophyll until uredinia formation, supporting their role in nutrient acquisition during biotrophic growth. Finally, CAZymes and lipase transcripts were predominantly expressed at late stages of infection, highlighting their importance during sporulation.
DOI: 10.1094/MPMI-01-11-0006
PubMed: 21644839
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pubmed:21644839Le document en format XML
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first="Emilie" last="Tisserant">Emilie Tisserant</name></author><author><name sortKey="Frey, Pascal" sort="Frey, Pascal" uniqKey="Frey P" first="Pascal" last="Frey">Pascal Frey</name></author><author><name sortKey="Martin, Francis" sort="Martin, Francis" uniqKey="Martin F" first="Francis" last="Martin">Francis Martin</name></author><author><name sortKey="Kohler, Annegret" sort="Kohler, Annegret" uniqKey="Kohler A" first="Annegret" last="Kohler">Annegret Kohler</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21644839</idno><idno type="pmid">21644839</idno><idno type="doi">10.1094/MPMI-01-11-0006</idno><idno type="wicri:Area/PubMed/Corpus">000104</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000104</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Melampsora larici-populina transcript profiling during germination and timecourse infection of poplar leaves reveals dynamic expression patterns associated with virulence and biotrophy.</title><author><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation><nlm:affiliation>Institut National de la Recherche Agronomique, Champenoux, France. duplessi@nancy.inra.fr</nlm:affiliation></affiliation></author><author><name sortKey="Hacquard, Stephane" sort="Hacquard, Stephane" uniqKey="Hacquard S" first="Stéphane" last="Hacquard">Stéphane Hacquard</name></author><author><name sortKey="Delaruelle, Christine" sort="Delaruelle, Christine" uniqKey="Delaruelle C" first="Christine" last="Delaruelle">Christine Delaruelle</name></author><author><name sortKey="Tisserant, Emilie" sort="Tisserant, Emilie" uniqKey="Tisserant E" first="Emilie" last="Tisserant">Emilie Tisserant</name></author><author><name sortKey="Frey, Pascal" sort="Frey, Pascal" uniqKey="Frey P" 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Sequence Analysis (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Populus (microbiology)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Spores, Fungal (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Basidiomycota</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Fungal</term><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Melampsora larici-populina is responsible for poplar leaf rust disease and causes severe epidemics in poplar plantations in Europe. The poplar rust genome has been recently sequenced and, in order to find the genetic determinants associated with its biotrophic lifestyle, we generated a whole-genome custom oligoarray and analyzed transcript profiles of M. larici-populina during the infection timecourse in poplar leaves. Different stages were investigated during the asexual development of the rust fungus, including resting and germinating urediniospores and seven in planta stages in the telial host. In total, 76% of the transcripts were detected during leaf infection as well as in urediniospores, whereas 20% were only detected in planta, including several transporters and many small secreted proteins (SSP). We focused our analysis on gene categories known to be related to plant colonization and biotrophic growth in rust pathogens, such as SSP, carbohydrate active enzymes (CAZymes), transporters, lipases, and proteases. Distinct sets of SSP transcripts were expressed all along the infection process, suggesting highly dynamic expression of candidate rust effectors. In contrast, transcripts encoding transporters and proteases were mostly expressed after 48 h postinoculation, when numerous haustoria are already formed in the leaf mesophyll until uredinia formation, supporting their role in nutrient acquisition during biotrophic growth. Finally, CAZymes and lipase transcripts were predominantly expressed at late stages of infection, highlighting their importance during sporulation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21644839</PMID><DateCompleted><Year>2011</Year><Month>12</Month><Day>13</Day></DateCompleted><DateRevised><Year>2011</Year><Month>06</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0894-0282</ISSN><JournalIssue CitedMedium="Print"><Volume>24</Volume><Issue>7</Issue><PubDate><Year>2011</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Molecular plant-microbe interactions : MPMI</Title><ISOAbbreviation>Mol Plant Microbe Interact</ISOAbbreviation></Journal><ArticleTitle>Melampsora larici-populina transcript profiling during germination and timecourse infection of poplar leaves reveals dynamic expression patterns associated with virulence and biotrophy.</ArticleTitle><Pagination><MedlinePgn>808-18</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/MPMI-01-11-0006</ELocationID><Abstract><AbstractText>Melampsora larici-populina is responsible for poplar leaf rust disease and causes severe epidemics in poplar plantations in Europe. The poplar rust genome has been recently sequenced and, in order to find the genetic determinants associated with its biotrophic lifestyle, we generated a whole-genome custom oligoarray and analyzed transcript profiles of M. larici-populina during the infection timecourse in poplar leaves. Different stages were investigated during the asexual development of the rust fungus, including resting and germinating urediniospores and seven in planta stages in the telial host. In total, 76% of the transcripts were detected during leaf infection as well as in urediniospores, whereas 20% were only detected in planta, including several transporters and many small secreted proteins (SSP). We focused our analysis on gene categories known to be related to plant colonization and biotrophic growth in rust pathogens, such as SSP, carbohydrate active enzymes (CAZymes), transporters, lipases, and proteases. Distinct sets of SSP transcripts were expressed all along the infection process, suggesting highly dynamic expression of candidate rust effectors. In contrast, transcripts encoding transporters and proteases were mostly expressed after 48 h postinoculation, when numerous haustoria are already formed in the leaf mesophyll until uredinia formation, supporting their role in nutrient acquisition during biotrophic growth. 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