The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.
Identifieur interne : 000040 ( PubMed/Corpus ); précédent : 000039; suivant : 000041The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.
Auteurs : Yuanzhong Jiang ; Li Guo ; Xiaodong Ma ; Xin Zhao ; Bo Jiao ; Chaofeng Li ; Keming LuoSource :
- Tree physiology [ 1758-4469 ] ; 2017.
English descriptors
- KwdEn :
- Basidiomycota (pathogenicity), Disease Resistance (genetics), Gene Expression Regulation, Plant (MeSH), Plant Diseases (genetics), Plant Diseases (microbiology), Plant Proteins (genetics), Plant Proteins (physiology), Plants, Genetically Modified (genetics), Plants, Genetically Modified (microbiology), Populus (genetics), Populus (microbiology), Salicylic Acid (MeSH), Transcription Factors (genetics), Transcription Factors (physiology).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- genetics : Disease Resistance, Plant Diseases, Plants, Genetically Modified, Populus.
- microbiology : Plant Diseases, Plants, Genetically Modified, Populus.
- pathogenicity : Basidiomycota.
- chemical , physiology : Plant Proteins, Transcription Factors.
- Gene Expression Regulation, Plant, Salicylic Acid.
Abstract
WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.
DOI: 10.1093/treephys/tpx008
PubMed: 28338710
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Guo</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Xiaodong" sort="Ma, Xiaodong" uniqKey="Ma X" first="Xiaodong" last="Ma">Xiaodong Ma</name><affiliation><nlm:affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, No. 3, Bayi Mid Road, Xining, Qinghai 810007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Xin" sort="Zhao, Xin" uniqKey="Zhao X" first="Xin" last="Zhao">Xin Zhao</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiao, Bo" sort="Jiao, Bo" uniqKey="Jiao B" first="Bo" last="Jiao">Bo Jiao</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Chaofeng" sort="Li, Chaofeng" uniqKey="Li C" first="Chaofeng" last="Li">Chaofeng Li</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28338710</idno><idno type="pmid">28338710</idno><idno type="doi">10.1093/treephys/tpx008</idno><idno type="wicri:Area/PubMed/Corpus">000040</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000040</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.</title><author><name sortKey="Jiang, Yuanzhong" sort="Jiang, Yuanzhong" uniqKey="Jiang Y" first="Yuanzhong" last="Jiang">Yuanzhong Jiang</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, No. 24, South Section 1, Yihuan Road, Chengdu 610065, China.</nlm:affiliation></affiliation></author><author><name sortKey="Guo, Li" sort="Guo, Li" uniqKey="Guo L" first="Li" last="Guo">Li Guo</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Ma, Xiaodong" sort="Ma, Xiaodong" uniqKey="Ma X" first="Xiaodong" last="Ma">Xiaodong Ma</name><affiliation><nlm:affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, No. 3, Bayi Mid Road, Xining, Qinghai 810007, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhao, Xin" sort="Zhao, Xin" uniqKey="Zhao X" first="Xin" last="Zhao">Xin Zhao</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Jiao, Bo" sort="Jiao, Bo" uniqKey="Jiao B" first="Bo" last="Jiao">Bo Jiao</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, Chaofeng" sort="Li, Chaofeng" uniqKey="Li C" first="Chaofeng" last="Li">Chaofeng Li</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</nlm:affiliation></affiliation></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name><affiliation><nlm:affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Basidiomycota (pathogenicity)</term><term>Disease Resistance (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (physiology)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (microbiology)</term><term>Populus (genetics)</term><term>Populus (microbiology)</term><term>Salicylic Acid (MeSH)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Disease Resistance</term><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Salicylic Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">28338710</PMID><DateCompleted><Year>2018</Year><Month>01</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>02</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>37</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>05</Month><Day>01</Day></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.</ArticleTitle><Pagination><MedlinePgn>665-675</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tpx008</ELocationID><Abstract><AbstractText>WRKY transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. In a previous study, we have shown that poplar WRKY89 is induced by salicylic acid (SA) treatment and plays an important role in resistance against fungi in transgenic poplars. Here, we determined an increase in transcript levels of Group IIa WRKY members in transgenic poplars overexpressing WRKY89 using quantitative real-time polymerase chain reaction analysis. Yeast one-hybrid assay showed that PtrWRKY18 and PtrWRKY35 were potential target genes of WRKY89. Furthermore, we demonstrated that PtrWRKY18 and PtrWRKY35 were localized in the nucleus, and exhibited no transcription activation activity. Constitutive overexpression of PtrWRKY18 and PtrWRKY35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen Melampsora. The results also provided support for the involvement of SA-mediated signaling in Melampsora resistance.</AbstractText><CopyrightInformation>© The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Yuanzhong</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>MOE Key Laboratory of Bio-Resources and Eco-Environment, College of Life Science, Sichuan University, No. 24, South Section 1, Yihuan Road, Chengdu 610065, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Li</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Xiaodong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Chemistry and Chemical Engineering, Qinghai University for Nationalities, No. 3, Bayi Mid Road, Xining, Qinghai 810007, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Xin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Bo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chaofeng</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, No. 23, Xinning Road, Xining, Qinghai 810008, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Keming</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Chongqing Key Laboratory of Transgenic Plant and Safety Control, Institute of Resources Botany, School of Life Sciences, Southwest University, No. 1, Tiansheng Road, Beibei, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001487" MajorTopicYN="N">Basidiomycota</DescriptorName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">CRISPER/Cas9</Keyword><Keyword MajorTopicYN="Y">Populus</Keyword><Keyword MajorTopicYN="Y">WRKY transcription factor</Keyword><Keyword MajorTopicYN="Y">fungal pathogen</Keyword><Keyword MajorTopicYN="Y">plant defense</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>07</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>01</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28338710</ArticleId><ArticleId IdType="pii">2996586</ArticleId><ArticleId IdType="doi">10.1093/treephys/tpx008</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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