Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar
Identifieur interne : 000588 ( Pmc/Curation ); précédent : 000587; suivant : 000589Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar
Auteurs : Nicole J. Dafoe [Canada, États-Unis] ; Brent E. Gowen [Canada] ; C Peter Constabel [Canada]Source :
- BMC Plant Biology [ 1471-2229 ] ; 2010.
Abstract
Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (
Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.
TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.
Url:
DOI: 10.1186/1471-2229-10-191
PubMed: 20796310
PubMed Central: 2956541
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Dafoe</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Centre for Forest Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I3">Agricultural Research Service, US Department of Agriculture, Gainesville, FL, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Agricultural Research Service, US Department of Agriculture, Gainesville, FL</wicri:regionArea></affiliation></author><author><name sortKey="Gowen, Brent E" sort="Gowen, Brent E" uniqKey="Gowen B" first="Brent E" last="Gowen">Brent E. Gowen</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Centre for Forest Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20796310</idno><idno type="pmc">2956541</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2956541</idno><idno type="RBID">PMC:2956541</idno><idno type="doi">10.1186/1471-2229-10-191</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000588</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000588</idno><idno type="wicri:Area/Pmc/Curation">000588</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000588</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar</title><author><name sortKey="Dafoe, Nicole J" sort="Dafoe, Nicole J" uniqKey="Dafoe N" first="Nicole J" last="Dafoe">Nicole J. Dafoe</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Centre for Forest Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I3">Agricultural Research Service, US Department of Agriculture, Gainesville, FL, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Agricultural Research Service, US Department of Agriculture, Gainesville, FL</wicri:regionArea></affiliation></author><author><name sortKey="Gowen, Brent E" sort="Gowen, Brent E" uniqKey="Gowen B" first="Brent E" last="Gowen">Brent E. Gowen</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="I2">Centre for Forest Biology, University of Victoria, Victoria, BC, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea>Centre for Forest Biology, University of Victoria, Victoria, BC</wicri:regionArea></affiliation></author></analytic><series><title level="j">BMC Plant Biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (<italic>Populus trichocarpa </italic>× <italic>P. deltoides) </italic>using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</p></sec><sec><title>Results</title><p>Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</p></sec><sec><title>Conclusions</title><p>TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Dafoe, Nj" uniqKey="Dafoe N">NJ Dafoe</name></author><author><name sortKey="Zamani, A" uniqKey="Zamani A">A Zamani</name></author><author><name sortKey="Ekramoddoullah, Akm" uniqKey="Ekramoddoullah A">AKM Ekramoddoullah</name></author><author><name sortKey="Lippert, D" uniqKey="Lippert D">D Lippert</name></author><author><name sortKey="Bohlmann, J" uniqKey="Bohlmann J">J Bohlmann</name></author><author><name sortKey="Constabel, Cp" uniqKey="Constabel C">CP Constabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Selitrennikoff, Cp" uniqKey="Selitrennikoff C">CP 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<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Plant Biol</journal-id><journal-title-group><journal-title>BMC Plant Biology</journal-title></journal-title-group><issn pub-type="epub">1471-2229</issn><publisher><publisher-name>BioMed Central</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20796310</article-id><article-id pub-id-type="pmc">2956541</article-id><article-id pub-id-type="publisher-id">1471-2229-10-191</article-id><article-id pub-id-type="doi">10.1186/1471-2229-10-191</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar</article-title></title-group><contrib-group><contrib contrib-type="author" id="A1"><name><surname>Dafoe</surname><given-names>Nicole J</given-names></name><xref ref-type="aff" rid="I1">1</xref><xref ref-type="aff" rid="I2">2</xref><xref ref-type="aff" rid="I3">3</xref><email>Nicole.Dafoe@ARS.USDA.GOV</email></contrib><contrib contrib-type="author" id="A2"><name><surname>Gowen</surname><given-names>Brent E</given-names></name><xref ref-type="aff" rid="I1">1</xref><email>bgowen@uvic.ca</email></contrib><contrib contrib-type="author" corresp="yes" id="A3"><name><surname>Constabel</surname><given-names>C Peter</given-names></name><xref ref-type="aff" rid="I1">1</xref><xref ref-type="aff" rid="I2">2</xref><email>cpc@uvic.ca</email></contrib></contrib-group><aff id="I1"><label>1</label>Department of Biology, University of Victoria, Victoria, BC, Canada</aff><aff id="I2"><label>2</label>Centre for Forest Biology, University of Victoria, Victoria, BC, Canada</aff><aff id="I3"><label>3</label>Agricultural Research Service, US Department of Agriculture, Gainesville, FL, USA</aff><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>26</day><month>8</month><year>2010</year></pub-date><volume>10</volume><fpage>191</fpage><lpage>191</lpage><history><date date-type="received"><day>25</day><month>9</month><year>2009</year></date><date date-type="accepted"><day>26</day><month>8</month><year>2010</year></date></history><permissions><copyright-statement>Copyright ©2010 Dafoe et al; licensee BioMed Central Ltd.</copyright-statement><copyright-year>2010</copyright-year><copyright-holder>Dafoe et al; licensee BioMed Central Ltd.</copyright-holder><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/2.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:href="http://www.biomedcentral.com/1471-2229/10/191"></self-uri><abstract><sec><title>Background</title><p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (<italic>Populus trichocarpa </italic>× <italic>P. deltoides) </italic>using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</p></sec><sec><title>Results</title><p>Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</p></sec><sec><title>Conclusions</title><p>TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.</p></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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