Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Positive selection in <italic>AvrP4</italic> avirulence gene homologues across the genus <italic>Melampsora</italic></title><author><name sortKey="Van Der Merwe, Marlien M" sort="Van Der Merwe, Marlien M" uniqKey="Van Der Merwe M" first="Marlien M." last="Van Der Merwe">Marlien M. Van Der Merwe</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Botanic Gardens Trust, National Herbarium of New South Wales</institution>,<addr-line>Sydney, New South Wales 2000, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kinnear, Mark W" sort="Kinnear, Mark W" uniqKey="Kinnear M" first="Mark W." last="Kinnear">Mark W. Kinnear</name><affiliation><nlm:aff id="aff3"><institution>The School of Biology, Australian National University</institution>,<addr-line>Canberra, Australian Capital Territory 2601, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Barrett, Luke G" sort="Barrett, Luke G" uniqKey="Barrett L" first="Luke G." last="Barrett">Luke G. Barrett</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>The School of Biology, Australian National University</institution>,<addr-line>Canberra, Australian Capital Territory 2601, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Department of Ecology and Evolution, University of Chicago</institution>,<addr-line>Chicago, IL 60637, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ericson, Lars" sort="Ericson, Lars" uniqKey="Ericson L" first="Lars" last="Ericson">Lars Ericson</name><affiliation><nlm:aff id="aff5"><institution>Department of Ecology and Environmental Science, Umeå University</institution>,<addr-line>90187 Umeå, Sweden</addr-line></nlm:aff></affiliation></author><author><name sortKey="Thrall, Peter H" sort="Thrall, Peter H" uniqKey="Thrall P" first="Peter H." last="Thrall">Peter H. Thrall</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Burdon, Jeremy J" sort="Burdon, Jeremy J" uniqKey="Burdon J" first="Jeremy J." last="Burdon">Jeremy J. Burdon</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19457888</idno><idno type="pmc">2817207</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2817207</idno><idno type="RBID">PMC:2817207</idno><idno type="doi">10.1098/rspb.2009.0328</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000503</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000503</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Positive selection in <italic>AvrP4</italic> avirulence gene homologues across the genus <italic>Melampsora</italic></title><author><name sortKey="Van Der Merwe, Marlien M" sort="Van Der Merwe, Marlien M" uniqKey="Van Der Merwe M" first="Marlien M." last="Van Der Merwe">Marlien M. Van Der Merwe</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff2"><institution>Botanic Gardens Trust, National Herbarium of New South Wales</institution>,<addr-line>Sydney, New South Wales 2000, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kinnear, Mark W" sort="Kinnear, Mark W" uniqKey="Kinnear M" first="Mark W." last="Kinnear">Mark W. Kinnear</name><affiliation><nlm:aff id="aff3"><institution>The School of Biology, Australian National University</institution>,<addr-line>Canberra, Australian Capital Territory 2601, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Barrett, Luke G" sort="Barrett, Luke G" uniqKey="Barrett L" first="Luke G." last="Barrett">Luke G. Barrett</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff3"><institution>The School of Biology, Australian National University</institution>,<addr-line>Canberra, Australian Capital Territory 2601, Australia</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="aff4"><institution>Department of Ecology and Evolution, University of Chicago</institution>,<addr-line>Chicago, IL 60637, USA</addr-line></nlm:aff></affiliation></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Ericson, Lars" sort="Ericson, Lars" uniqKey="Ericson L" first="Lars" last="Ericson">Lars Ericson</name><affiliation><nlm:aff id="aff5"><institution>Department of Ecology and Environmental Science, Umeå University</institution>,<addr-line>90187 Umeå, Sweden</addr-line></nlm:aff></affiliation></author><author><name sortKey="Thrall, Peter H" sort="Thrall, Peter H" uniqKey="Thrall P" first="Peter H." last="Thrall">Peter H. Thrall</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author><author><name sortKey="Burdon, Jeremy J" sort="Burdon, Jeremy J" uniqKey="Burdon J" first="Jeremy J." last="Burdon">Jeremy J. Burdon</name><affiliation><nlm:aff id="aff1"><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the Royal Society B: Biological Sciences</title><idno type="ISSN">0962-8452</idno><idno type="eISSN">1471-2954</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Pathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, <italic>AvrP4</italic>, in the plant pathogen <italic>Melampsora lini</italic>, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the <italic>Melampsora</italic> species. We amplified <italic>AvrP4</italic> homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the <italic>AvrP4</italic> sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus non-synonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Biol Sci</journal-id><journal-id journal-id-type="hwp">royprsb</journal-id><journal-title-group><journal-title>Proceedings of the Royal Society B: Biological Sciences</journal-title></journal-title-group><issn pub-type="ppub">0962-8452</issn><issn pub-type="epub">1471-2954</issn><publisher><publisher-name>The Royal Society</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19457888</article-id><article-id pub-id-type="pmc">2817207</article-id><article-id pub-id-type="publisher-id">rspb20090328</article-id><article-id pub-id-type="doi">10.1098/rspb.2009.0328</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research articles</subject></subj-group><subj-group subj-group-type="hwp-journal-coll"><subject>1001</subject><subject>70</subject></subj-group></article-categories><title-group><article-title>Positive selection in <italic>AvrP4</italic> avirulence gene homologues across the genus <italic>Melampsora</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Van der Merwe</surname><given-names>Marlien M.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Kinnear</surname><given-names>Mark W.</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Barrett</surname><given-names>Luke G.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Dodds</surname><given-names>Peter N.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ericson</surname><given-names>Lars</given-names></name><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Thrall</surname><given-names>Peter H.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Burdon</surname><given-names>Jeremy J.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1"><label>1</label><institution>CSIRO-Plant Industry</institution>,<addr-line>GPO Box 1600, Canberra, Australian Capital Territory 2600, Australia</addr-line></aff><aff id="aff2"><label>2</label><institution>Botanic Gardens Trust, National Herbarium of New South Wales</institution>,<addr-line>Sydney, New South Wales 2000, Australia</addr-line></aff><aff id="aff3"><label>3</label><institution>The School of Biology, Australian National University</institution>,<addr-line>Canberra, Australian Capital Territory 2601, Australia</addr-line></aff><aff id="aff4"><label>4</label><institution>Department of Ecology and Evolution, University of Chicago</institution>,<addr-line>Chicago, IL 60637, USA</addr-line></aff><aff id="aff5"><label>5</label><institution>Department of Ecology and Environmental Science, Umeå University</institution>,<addr-line>90187 Umeå, Sweden</addr-line></aff><author-notes><corresp id="cor1"><label>*</label>Author for correspondence (<email>marlien.vandermerwe@gmail.com</email>)</corresp></author-notes><pub-date pub-type="ppub"><day>22</day><month>8</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>20</day><month>5</month><year>2009</year></pub-date><volume>276</volume><issue>1669</issue><fpage>2913</fpage><lpage>2922</lpage><history><date date-type="received"><day>25</day><month>2</month><year>2009</year></date><date date-type="accepted"><day>21</day><month>4</month><year>2009</year></date></history><permissions><copyright-statement>© 2009 The Royal Society</copyright-statement><copyright-year>2009</copyright-year></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="2913.pdf"></self-uri><abstract><p>Pathogen genes involved in interactions with their plant hosts are expected to evolve under positive Darwinian selection or balancing selection. In this study a single copy avirulence gene, <italic>AvrP4</italic>, in the plant pathogen <italic>Melampsora lini</italic>, was used to investigate the evolution of such a gene across species. Partial translation elongation factor 1-alpha sequences were obtained to establish phylogenetic relationships among the <italic>Melampsora</italic> species. We amplified <italic>AvrP4</italic> homologues from species pathogenic on hosts from different plant families and orders, across the inferred phylogeny. Translations of the <italic>AvrP4</italic> sequences revealed a predicted signal peptide and towards the C-terminus of the protein, six identically spaced cysteines were identified in all sequences. Maximum likelihood analysis of synonymous versus non-synonymous substitution rates indicated that positive selection played a role in the evolution of the gene during the diversification of the genus. Fourteen codons under significant positive selection reside in the C-terminal 28 amino acid region, suggesting that this region interacts with host molecules in most sequenced accessions. Selection pressures on the gene may be either due to the pathogenicity or avirulence function of the gene or both.</p></abstract><kwd-group><kwd><italic>Melampsora</italic></kwd><kwd>rust fungi</kwd><kwd>pathogen</kwd><kwd>avirulence</kwd><kwd>effector</kwd><kwd>selection</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000503 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000503 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.38. |  |