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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM</title><author><name sortKey="Ve, Thomas" sort="Ve, Thomas" uniqKey="Ve T" first="Thomas" last="Ve">Thomas Ve</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="J Williams, Simon" sort="J Williams, Simon" uniqKey="J Williams S" first="Simon" last="J. Williams">Simon J. Williams</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Stamp, Anna" sort="Stamp, Anna" uniqKey="Stamp A" first="Anna" last="Stamp">Anna Stamp</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Valkov, Eugene" sort="Valkov, Eugene" uniqKey="Valkov E" first="Eugene" last="Valkov">Eugene Valkov</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><affiliation><nlm:aff id="b"><institution>CSIRO Plant Industry</institution>, Canberra, Australian Capital Territory 2601,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Anderson, Peter A" sort="Anderson, Peter A" uniqKey="Anderson P" first="Peter A." last="Anderson">Peter A. Anderson</name><affiliation><nlm:aff id="c">The School of Biological Sciences,<institution>Flinders University</institution>, Adelaide,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22139177</idno><idno type="pmc">3232150</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232150</idno><idno type="RBID">PMC:3232150</idno><idno type="doi">10.1107/S1744309111037675</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">000422</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000422</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM</title><author><name sortKey="Ve, Thomas" sort="Ve, Thomas" uniqKey="Ve T" first="Thomas" last="Ve">Thomas Ve</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="J Williams, Simon" sort="J Williams, Simon" uniqKey="J Williams S" first="Simon" last="J. Williams">Simon J. Williams</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Stamp, Anna" sort="Stamp, Anna" uniqKey="Stamp A" first="Anna" last="Stamp">Anna Stamp</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Valkov, Eugene" sort="Valkov, Eugene" uniqKey="Valkov E" first="Eugene" last="Valkov">Eugene Valkov</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name><affiliation><nlm:aff id="b"><institution>CSIRO Plant Industry</institution>, Canberra, Australian Capital Territory 2601,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Anderson, Peter A" sort="Anderson, Peter A" uniqKey="Anderson P" first="Peter A." last="Anderson">Peter A. Anderson</name><affiliation><nlm:aff id="c">The School of Biological Sciences,<institution>Flinders University</institution>, Adelaide,<country>Australia</country></nlm:aff></affiliation></author><author><name sortKey="Kobe, Bostjan" sort="Kobe, Bostjan" uniqKey="Kobe B" first="Bostjan" last="Kobe">Bostjan Kobe</name><affiliation><nlm:aff id="a">School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></nlm:aff></affiliation></author></analytic><series><title level="j">Acta Crystallographica Section F: Structural Biology and Crystallization Communications</title><idno type="eISSN">1744-3091</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>This report describes the crystallization and X-ray diffraction analysis of two different allelic variants of the AvrM effector from flax rust.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Crystallogr Sect F Struct Biol Cryst Commun</journal-id><journal-id journal-id-type="publisher-id">Acta Cryst. F</journal-id><journal-title-group><journal-title>Acta Crystallographica Section F: Structural Biology and Crystallization Communications</journal-title></journal-title-group><issn pub-type="epub">1744-3091</issn><publisher><publisher-name>International Union of Crystallography</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22139177</article-id><article-id pub-id-type="pmc">3232150</article-id><article-id pub-id-type="publisher-id">uo5031</article-id><article-id pub-id-type="doi">10.1107/S1744309111037675</article-id><article-id pub-id-type="coden">ACSFCL</article-id><article-id pub-id-type="pii">S1744309111037675</article-id><article-categories><subj-group subj-group-type="heading"><subject>Crystallization Communications</subject></subj-group></article-categories><title-group><article-title>Crystallization and X-ray diffraction analysis of the C-terminal domain of the flax rust effector protein AvrM</article-title><alt-title>AvrM</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ve</surname><given-names>Thomas</given-names></name><xref ref-type="aff" rid="a">a</xref><xref ref-type="corresp" rid="cor">*</xref></contrib><contrib contrib-type="author"><name><surname>J. Williams</surname><given-names>Simon</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Stamp</surname><given-names>Anna</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><contrib contrib-type="author"><name><surname>Valkov</surname><given-names>Eugene</given-names></name><xref ref-type="aff" rid="a">a</xref><xref ref-type="author-notes" rid="aunote1">‡</xref></contrib><contrib contrib-type="author"><name><surname>Dodds</surname><given-names>Peter N.</given-names></name><xref ref-type="aff" rid="b">b</xref></contrib><contrib contrib-type="author"><name><surname>Anderson</surname><given-names>Peter A.</given-names></name><xref ref-type="aff" rid="c">c</xref></contrib><contrib contrib-type="author"><name><surname>Kobe</surname><given-names>Bostjan</given-names></name><xref ref-type="aff" rid="a">a</xref></contrib><aff id="a"><label>a</label>School of Chemistry and Molecular Biosciences, Institute for Molecular Bioscience (Division of Chemistry and Structural Biology) and Centre for Infectious Disease Research,<institution>University of Queensland</institution>, Brisbane, Queensland 4072,<country>Australia</country></aff><aff id="b"><label>b</label><institution>CSIRO Plant Industry</institution>, Canberra, Australian Capital Territory 2601,<country>Australia</country></aff><aff id="c"><label>c</label>The School of Biological Sciences,<institution>Flinders University</institution>, Adelaide,<country>Australia</country></aff></contrib-group><author-notes><corresp id="cor">Correspondence e-mail: <email>t.ve@uq.edu.au</email></corresp><fn id="aunote1"><label>‡</label><p>Current address: Department of Biochemistry, Sanger Building, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, England.</p></fn></author-notes><pub-date pub-type="collection"><day>1</day><month>12</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>26</day><month>11</month><year>2011</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>12</month><year>2013</year></pub-date><pmc-comment> PMC Release delay is 24 months and 0 days and was based on the
<volume>67</volume><issue>Pt 12</issue><issue-id pub-id-type="publisher-id">f111200</issue-id><fpage>1603</fpage><lpage>1607</lpage><history><date date-type="received"><day>11</day><month>8</month><year>2011</year></date><date date-type="accepted"><day>15</day><month>9</month><year>2011</year></date></history><permissions><copyright-statement>© International Union of Crystallography 2011</copyright-statement><copyright-year>2011</copyright-year></permissions><self-uri xlink:type="simple" xlink:href="http://dx.doi.org/10.1107/S1744309111037675">A full version of this article is available from Crystallography Journals Online.</self-uri><abstract abstract-type="toc"><p>This report describes the crystallization and X-ray diffraction analysis of two different allelic variants of the AvrM effector from flax rust.</p></abstract><abstract><p>The flax rust effector AvrM is a secreted protein of unknown fold that is recognized by the M resistance protein in flax. In order to investigate the structural basis of the AvrM–M interaction and possible virulence-associated functions of AvrM, the C-terminal domains of two different AvrM variants (AvrM-A and avrM) were crystallized. Crystals of native AvrM-A were obtained using pentaerythritol ethoxylate (15/4 EO/OH) as a precipitant and diffracted X-rays to 2.9 Å resolution. Selenomethionine-derivative crystals of similar quality were obtained using PEG 1500 as a precipitant. Both the native and selenomethionine-labelled AvrM-A crystals had symmetry of space group <italic>C</italic>222<sub>1</sub> with eight molecules in the asymmetric unit. Crystals of avrM had symmetry of space group <italic>P</italic>2<sub>1</sub>2<sub>1</sub>2<sub>1</sub> and diffracted X-rays to 2.7 Å resolution. Initial AvrM-A phases were calculated using the single-wavelength anomalous dispersion (SAD) method and a partial model was built. Phases for avrM were obtained by molecular replacement using the partial AvrM-A model.</p></abstract><kwd-group><kwd>AvrM</kwd><kwd>fungal effectors</kwd><kwd>plant disease resistance</kwd><kwd>single-wavelength anomalous dispersion</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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