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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transformation of the flax rust fungus, <italic>Melampsora lini</italic>: selection via silencing of an avirulence gene</title><author><name sortKey="Lawrence, Gregory J" sort="Lawrence, Gregory J" uniqKey="Lawrence G" first="Gregory J." last="Lawrence">Gregory J. Lawrence</name></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name></author><author><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. Ellis</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19874543</idno><idno type="pmc">3142615</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3142615</idno><idno type="RBID">PMC:3142615</idno><idno type="doi">10.1111/j.1365-313X.2009.04052.x</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000389</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000389</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transformation of the flax rust fungus, <italic>Melampsora lini</italic>: selection via silencing of an avirulence gene</title><author><name sortKey="Lawrence, Gregory J" sort="Lawrence, Gregory J" uniqKey="Lawrence G" first="Gregory J." last="Lawrence">Gregory J. Lawrence</name></author><author><name sortKey="Dodds, Peter N" sort="Dodds, Peter N" uniqKey="Dodds P" first="Peter N." last="Dodds">Peter N. Dodds</name></author><author><name sortKey="Ellis, Jeffrey G" sort="Ellis, Jeffrey G" uniqKey="Ellis J" first="Jeffrey G." last="Ellis">Jeffrey G. Ellis</name></author></analytic><series><title level="j">The Plant journal : for cell and molecular biology</title><idno type="ISSN">0960-7412</idno><idno type="eISSN">1365-313X</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>SUMMARY</title><p id="P2">Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an <italic>Agrobacterium</italic>-mediated genetic transformation procedure for the model flax rust fungus <italic>Melampsora lini</italic>, which infects flax (<italic>Linum usitatissimum</italic>). Selection of transgenic rust lines is based on silencing of <italic>AvrL567</italic>, which encodes a rust effector protein that is recognised by the flax <italic>L6</italic> immune receptor. The non-transgenic rust line is unable to infect flax plants expressing <italic>L6</italic>, while silenced transgenic lines are virulent on these plants, providing an effective selection system. This directly confirms that the cloned <italic>AvrL567</italic> gene is responsible for flax rust virulence phenotypes, and demonstrates the utility of this system to probe rust gene function.</p></div></front></TEI><pmc article-type="research-article" xml:lang="en"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9207397</journal-id><journal-id journal-id-type="pubmed-jr-id">2425</journal-id><journal-id journal-id-type="nlm-ta">Plant J</journal-id><journal-title-group><journal-title>The Plant journal : for cell and molecular biology</journal-title></journal-title-group><issn pub-type="ppub">0960-7412</issn><issn pub-type="epub">1365-313X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">19874543</article-id><article-id pub-id-type="pmc">3142615</article-id><article-id pub-id-type="doi">10.1111/j.1365-313X.2009.04052.x</article-id><article-id pub-id-type="manuscript">NIHMS307118</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Transformation of the flax rust fungus, <italic>Melampsora lini</italic>: selection via silencing of an avirulence gene</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lawrence</surname><given-names>Gregory J.</given-names></name><xref rid="FN1" ref-type="author-notes">*</xref></contrib><contrib contrib-type="author"><name><surname>Dodds</surname><given-names>Peter N.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ellis</surname><given-names>Jeffrey G.</given-names></name></contrib><aff id="A1">CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia</aff></contrib-group><author-notes><corresp id="FN1"><label>*</label>For correspondence (fax +61 2 6246 5000; <email>greg.lawrence@csiro.au</email>)</corresp></author-notes><pub-date pub-type="nihms-submitted"><day>29</day><month>6</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>26</day><month>10</month><year>2009</year></pub-date><pub-date pub-type="ppub"><month>1</month><year>2010</year></pub-date><pub-date pub-type="pmc-release"><day>25</day><month>7</month><year>2011</year></pub-date><volume>61</volume><issue>2</issue><fpage>364</fpage><lpage>369</lpage><permissions><copyright-statement>© 2009 CSIRO</copyright-statement><copyright-year>2009</copyright-year></permissions><abstract><title>SUMMARY</title><p id="P2">Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an <italic>Agrobacterium</italic>-mediated genetic transformation procedure for the model flax rust fungus <italic>Melampsora lini</italic>, which infects flax (<italic>Linum usitatissimum</italic>). Selection of transgenic rust lines is based on silencing of <italic>AvrL567</italic>, which encodes a rust effector protein that is recognised by the flax <italic>L6</italic> immune receptor. The non-transgenic rust line is unable to infect flax plants expressing <italic>L6</italic>, while silenced transgenic lines are virulent on these plants, providing an effective selection system. This directly confirms that the cloned <italic>AvrL567</italic> gene is responsible for flax rust virulence phenotypes, and demonstrates the utility of this system to probe rust gene function.</p></abstract><kwd-group><kwd>rust</kwd><kwd>transformation</kwd><kwd>avirulence</kwd><kwd>effector</kwd><kwd>gene silencing</kwd><kwd><italic>Agrobacterium</italic></kwd></kwd-group><funding-group><award-group><funding-source country="United States">National Institute of General Medical Sciences : NIGMS</funding-source><award-id>R01 GM074265-01A2 || GM</award-id></award-group></funding-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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