Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by <italic>Phytophthora sojae effector</italic> Avr1b<xref ref-type="fn" rid="fn3">[W]</xref></title><author><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Kale, Shiv D" sort="Kale, Shiv D" uniqKey="Kale S" first="Shiv D." last="Kale">Shiv D. Kale</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Wang, Xinle" sort="Wang, Xinle" uniqKey="Wang X" first="Xinle" last="Wang">Xinle Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Chen, Yubo" sort="Chen, Yubo" uniqKey="Chen Y" first="Yubo" last="Chen">Yubo Chen</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Wang, Qunqing" sort="Wang, Qunqing" uniqKey="Wang Q" first="Qunqing" last="Wang">Qunqing Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Wang, Xia" sort="Wang, Xia" uniqKey="Wang X" first="Xia" last="Wang">Xia Wang</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Jiang, Rays H Y" sort="Jiang, Rays H Y" uniqKey="Jiang R" first="Rays H. Y." last="Jiang">Rays H. Y. Jiang</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Laboratory of Phytopathology, Wageningen University, NL-6709 PD Wageningen, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Arredondo, Felipe D" sort="Arredondo, Felipe D" uniqKey="Arredondo F" first="Felipe D." last="Arredondo">Felipe D. Arredondo</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Anderson, Ryan G" sort="Anderson, Ryan G" uniqKey="Anderson R" first="Ryan G." last="Anderson">Ryan G. Anderson</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Thakur, Poulami B" sort="Thakur, Poulami B" uniqKey="Thakur P" first="Poulami B." last="Thakur">Poulami B. Thakur</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Mcdowell, John M" sort="Mcdowell, John M" uniqKey="Mcdowell J" first="John M." last="Mcdowell">John M. Mcdowell</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Tyler, Brett M" sort="Tyler, Brett M" uniqKey="Tyler B" first="Brett M." last="Tyler">Brett M. Tyler</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18390593</idno><idno type="pmc">2390733</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2390733</idno><idno type="RBID">PMC:2390733</idno><idno type="doi">10.1105/tpc.107.057067</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000282</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000282</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by <italic>Phytophthora sojae effector</italic> Avr1b<xref ref-type="fn" rid="fn3">[W]</xref></title><author><name sortKey="Dou, Daolong" sort="Dou, Daolong" uniqKey="Dou D" first="Daolong" last="Dou">Daolong Dou</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Kale, Shiv D" sort="Kale, Shiv D" uniqKey="Kale S" first="Shiv D." last="Kale">Shiv D. Kale</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Wang, Xinle" sort="Wang, Xinle" uniqKey="Wang X" first="Xinle" last="Wang">Xinle Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Chen, Yubo" sort="Chen, Yubo" uniqKey="Chen Y" first="Yubo" last="Chen">Yubo Chen</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Wang, Qunqing" sort="Wang, Qunqing" uniqKey="Wang Q" first="Qunqing" last="Wang">Qunqing Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Wang, Xia" sort="Wang, Xia" uniqKey="Wang X" first="Xia" last="Wang">Xia Wang</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Jiang, Rays H Y" sort="Jiang, Rays H Y" uniqKey="Jiang R" first="Rays H. Y." last="Jiang">Rays H. Y. Jiang</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation><affiliation><nlm:aff id="aff3">Laboratory of Phytopathology, Wageningen University, NL-6709 PD Wageningen, The Netherlands</nlm:aff></affiliation></author><author><name sortKey="Arredondo, Felipe D" sort="Arredondo, Felipe D" uniqKey="Arredondo F" first="Felipe D." last="Arredondo">Felipe D. Arredondo</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Anderson, Ryan G" sort="Anderson, Ryan G" uniqKey="Anderson R" first="Ryan G." last="Anderson">Ryan G. Anderson</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Thakur, Poulami B" sort="Thakur, Poulami B" uniqKey="Thakur P" first="Poulami B." last="Thakur">Poulami B. Thakur</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Mcdowell, John M" sort="Mcdowell, John M" uniqKey="Mcdowell J" first="John M." last="Mcdowell">John M. Mcdowell</name><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author><author><name sortKey="Wang, Yuanchao" sort="Wang, Yuanchao" uniqKey="Wang Y" first="Yuanchao" last="Wang">Yuanchao Wang</name><affiliation><nlm:aff id="aff2">Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</nlm:aff></affiliation></author><author><name sortKey="Tyler, Brett M" sort="Tyler, Brett M" uniqKey="Tyler B" first="Brett M." last="Tyler">Brett M. Tyler</name><affiliation><nlm:aff id="aff1">Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation><affiliation><nlm:aff id="aff4">Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</nlm:aff></affiliation></author></analytic><series><title level="j">The Plant Cell</title><idno type="ISSN">1040-4651</idno><idno type="eISSN">1532-298X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The sequenced genomes of oomycete plant pathogens contain large superfamilies of effector proteins containing the protein translocation motif RXLR-dEER. However, the contributions of these effectors to pathogenicity remain poorly understood. Here, we show that the <italic>Phytophthora sojae</italic> effector protein Avr1b can contribute positively to virulence and can suppress programmed cell death (PCD) triggered by the mouse BAX protein in yeast, soybean (<italic>Glycine max</italic>), and <italic>Nicotiana benthamiana</italic> cells. We identify three conserved motifs (K, W, and Y) in the C terminus of the Avr1b protein and show that mutations in the conserved residues of the W and Y motifs reduce or abolish the ability of Avr1b to suppress PCD and also abolish the avirulence interaction of Avr1b with the <italic>Rps</italic>1b resistance gene in soybean. W and Y motifs are present in at least half of the identified oomycete RXLR-dEER effector candidates, and we show that three of these candidates also suppress PCD in soybean. Together, these results indicate that the W and Y motifs are critical for the interaction of Avr1b with host plant target proteins and support the hypothesis that these motifs are critical for the functions of the very large number of predicted oomycete effectors that contain them.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Cell</journal-id><journal-id journal-id-type="publisher-id">plantcell</journal-id><journal-title>The Plant Cell</journal-title><issn pub-type="ppub">1040-4651</issn><issn pub-type="epub">1532-298X</issn><publisher><publisher-name>American Society of Plant Biologists</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18390593</article-id><article-id pub-id-type="pmc">2390733</article-id><article-id pub-id-type="publisher-id">057067</article-id><article-id pub-id-type="doi">10.1105/tpc.107.057067</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Articles</subject></subj-group></article-categories><title-group><article-title>Conserved C-Terminal Motifs Required for Avirulence and Suppression of Cell Death by <italic>Phytophthora sojae effector</italic> Avr1b<xref ref-type="fn" rid="fn3">[W]</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dou</surname><given-names>Daolong</given-names></name><xref ref-type="aff" rid="aff1">a</xref><xref ref-type="fn" rid="fn1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kale</surname><given-names>Shiv D.</given-names></name><xref ref-type="aff" rid="aff1">a</xref><xref ref-type="fn" rid="fn1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xinle</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Yubo</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Qunqing</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xia</given-names></name><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Jiang</surname><given-names>Rays H.Y.</given-names></name><xref ref-type="aff" rid="aff1">a</xref><xref ref-type="aff" rid="aff3">c</xref><xref ref-type="fn" rid="fn2">2</xref></contrib><contrib contrib-type="author"><name><surname>Arredondo</surname><given-names>Felipe D.</given-names></name><xref ref-type="aff" rid="aff1">a</xref></contrib><contrib contrib-type="author"><name><surname>Anderson</surname><given-names>Ryan G.</given-names></name><xref ref-type="aff" rid="aff4">d</xref></contrib><contrib contrib-type="author"><name><surname>Thakur</surname><given-names>Poulami B.</given-names></name><xref ref-type="aff" rid="aff4">d</xref></contrib><contrib contrib-type="author"><name><surname>McDowell</surname><given-names>John M.</given-names></name><xref ref-type="aff" rid="aff4">d</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Yuanchao</given-names></name><xref ref-type="aff" rid="aff2">b</xref></contrib><contrib contrib-type="author"><name><surname>Tyler</surname><given-names>Brett M.</given-names></name><xref ref-type="aff" rid="aff1">a</xref><xref ref-type="aff" rid="aff4">d</xref><xref ref-type="corresp" rid="cor1">3</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</aff><aff id="aff2"><label>b</label>Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China</aff><aff id="aff3"><label>c</label>Laboratory of Phytopathology, Wageningen University, NL-6709 PD Wageningen, The Netherlands</aff><aff id="aff4"><label>d</label>Department of Plant Pathology, Physiology, and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061</aff><author-notes><fn id="fn1"><label>1</label><p>These authors contributed equally to this work.</p></fn><fn id="fn2"><label>2</label><p>Current address: Broad Institute, Massachusetts Institute of Technology, 7 Cambridge Center, Cambridge, MA 02142.</p></fn><fn id="cor1"><label>3</label><p>Address correspondence to <email>bmtyler@vt.edu</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2008</year></pub-date><volume>20</volume><issue>4</issue><fpage>1118</fpage><lpage>1133</lpage><history><date date-type="received"><day>20</day><month>11</month><year>2007</year></date><date date-type="rev-recd"><day>26</day><month>2</month><year>2008</year></date><date date-type="accepted"><day>17</day><month>3</month><year>2008</year></date></history><permissions><copyright-statement>Copyright © 2008, American Society of Plant Biologists</copyright-statement></permissions><abstract><p>The sequenced genomes of oomycete plant pathogens contain large superfamilies of effector proteins containing the protein translocation motif RXLR-dEER. However, the contributions of these effectors to pathogenicity remain poorly understood. Here, we show that the <italic>Phytophthora sojae</italic> effector protein Avr1b can contribute positively to virulence and can suppress programmed cell death (PCD) triggered by the mouse BAX protein in yeast, soybean (<italic>Glycine max</italic>), and <italic>Nicotiana benthamiana</italic> cells. We identify three conserved motifs (K, W, and Y) in the C terminus of the Avr1b protein and show that mutations in the conserved residues of the W and Y motifs reduce or abolish the ability of Avr1b to suppress PCD and also abolish the avirulence interaction of Avr1b with the <italic>Rps</italic>1b resistance gene in soybean. W and Y motifs are present in at least half of the identified oomycete RXLR-dEER effector candidates, and we show that three of these candidates also suppress PCD in soybean. Together, these results indicate that the W and Y motifs are critical for the interaction of Avr1b with host plant target proteins and support the hypothesis that these motifs are critical for the functions of the very large number of predicted oomycete effectors that contain them.</p></abstract></article-meta><notes><fn-group><fn><p>The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors (<ext-link ext-link-type="uri" xlink:href="www.plantcell.org">www.plantcell.org</ext-link>) is: Brett M. Tyler (<email>bmtyler@vt.edu</email>).</p></fn><fn id="fn3"><label>[W]</label><p>Online version contains Web-only data.</p></fn><fn><p><ext-link ext-link-type="uri" xlink:href="www.plantcell.org/cgi/doi/10.1105/tpc.107.057067">www.plantcell.org/cgi/doi/10.1105/tpc.107.057067</ext-link></p></fn></fn-group></notes></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000282 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000282 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.38. |  |