Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid
Identifieur interne : 000077 ( Pmc/Checkpoint ); précédent : 000076; suivant : 000078Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid
Auteurs : Maria L. Irigoyen [États-Unis] ; Danielle C. Garceau [États-Unis] ; Adriana Bohorquez-Chaux [Colombie] ; Luis Augusto Becerra Lopez-Lavalle [Colombie] ; Laura Perez-Fons [Royaume-Uni] ; Paul D. Fraser [Royaume-Uni] ; Linda L. Walling [États-Unis]Source :
- BMC Genomics [ 1471-2164 ] ; 2020.
Abstract
Whiteflies are a threat to cassava (
The cassava genome possesses 14 of the 17 plant
This study represents the first genome-wide characterization of
Url:
DOI: 10.1186/s12864-019-6443-1
PubMed: 31996126
PubMed Central: 6990599
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:6990599Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genome-wide analyses of cassava <italic>Pathogenesis-related</italic>
(<italic>PR</italic>
) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid</title>
<author><name sortKey="Irigoyen, Maria L" sort="Irigoyen, Maria L" uniqKey="Irigoyen M" first="Maria L." last="Irigoyen">Maria L. Irigoyen</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Garceau, Danielle C" sort="Garceau, Danielle C" uniqKey="Garceau D" first="Danielle C." last="Garceau">Danielle C. Garceau</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bohorquez Chaux, Adriana" sort="Bohorquez Chaux, Adriana" uniqKey="Bohorquez Chaux A" first="Adriana" last="Bohorquez-Chaux">Adriana Bohorquez-Chaux</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0943 556X</institution-id>
<institution-id institution-id-type="GRID">grid.418348.2</institution-id>
<institution>International Center of Tropical Agriculture (CIAT),</institution>
</institution-wrap>
Cali, Colombia</nlm:aff>
<country xml:lang="fr">Colombie</country>
<wicri:regionArea>Cali</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lopez Lavalle, Luis Augusto Becerra" sort="Lopez Lavalle, Luis Augusto Becerra" uniqKey="Lopez Lavalle L" first="Luis Augusto Becerra" last="Lopez-Lavalle">Luis Augusto Becerra Lopez-Lavalle</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0943 556X</institution-id>
<institution-id institution-id-type="GRID">grid.418348.2</institution-id>
<institution>International Center of Tropical Agriculture (CIAT),</institution>
</institution-wrap>
Cali, Colombia</nlm:aff>
<country xml:lang="fr">Colombie</country>
<wicri:regionArea>Cali</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Perez Fons, Laura" sort="Perez Fons, Laura" uniqKey="Perez Fons L" first="Laura" last="Perez-Fons">Laura Perez-Fons</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2188 881X</institution-id>
<institution-id institution-id-type="GRID">grid.4970.a</institution-id>
<institution>Department of Biological Sciences,</institution>
<institution>Royal Holloway University of London,</institution>
</institution-wrap>
Egham, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Egham</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fraser, Paul D" sort="Fraser, Paul D" uniqKey="Fraser P" first="Paul D." last="Fraser">Paul D. Fraser</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2188 881X</institution-id>
<institution-id institution-id-type="GRID">grid.4970.a</institution-id>
<institution>Department of Biological Sciences,</institution>
<institution>Royal Holloway University of London,</institution>
</institution-wrap>
Egham, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Egham</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Walling, Linda L" sort="Walling, Linda L" uniqKey="Walling L" first="Linda L." last="Walling">Linda L. Walling</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">31996126</idno>
<idno type="pmc">6990599</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6990599</idno>
<idno type="RBID">PMC:6990599</idno>
<idno type="doi">10.1186/s12864-019-6443-1</idno>
<date when="2020">2020</date>
<idno type="wicri:Area/Pmc/Corpus">000169</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000169</idno>
<idno type="wicri:Area/Pmc/Curation">000169</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000169</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000077</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000077</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genome-wide analyses of cassava <italic>Pathogenesis-related</italic>
(<italic>PR</italic>
) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid</title>
<author><name sortKey="Irigoyen, Maria L" sort="Irigoyen, Maria L" uniqKey="Irigoyen M" first="Maria L." last="Irigoyen">Maria L. Irigoyen</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Garceau, Danielle C" sort="Garceau, Danielle C" uniqKey="Garceau D" first="Danielle C." last="Garceau">Danielle C. Garceau</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Bohorquez Chaux, Adriana" sort="Bohorquez Chaux, Adriana" uniqKey="Bohorquez Chaux A" first="Adriana" last="Bohorquez-Chaux">Adriana Bohorquez-Chaux</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0943 556X</institution-id>
<institution-id institution-id-type="GRID">grid.418348.2</institution-id>
<institution>International Center of Tropical Agriculture (CIAT),</institution>
</institution-wrap>
Cali, Colombia</nlm:aff>
<country xml:lang="fr">Colombie</country>
<wicri:regionArea>Cali</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Lopez Lavalle, Luis Augusto Becerra" sort="Lopez Lavalle, Luis Augusto Becerra" uniqKey="Lopez Lavalle L" first="Luis Augusto Becerra" last="Lopez-Lavalle">Luis Augusto Becerra Lopez-Lavalle</name>
<affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0943 556X</institution-id>
<institution-id institution-id-type="GRID">grid.418348.2</institution-id>
<institution>International Center of Tropical Agriculture (CIAT),</institution>
</institution-wrap>
Cali, Colombia</nlm:aff>
<country xml:lang="fr">Colombie</country>
<wicri:regionArea>Cali</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Perez Fons, Laura" sort="Perez Fons, Laura" uniqKey="Perez Fons L" first="Laura" last="Perez-Fons">Laura Perez-Fons</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2188 881X</institution-id>
<institution-id institution-id-type="GRID">grid.4970.a</institution-id>
<institution>Department of Biological Sciences,</institution>
<institution>Royal Holloway University of London,</institution>
</institution-wrap>
Egham, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Egham</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Fraser, Paul D" sort="Fraser, Paul D" uniqKey="Fraser P" first="Paul D." last="Fraser">Paul D. Fraser</name>
<affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2188 881X</institution-id>
<institution-id institution-id-type="GRID">grid.4970.a</institution-id>
<institution>Department of Biological Sciences,</institution>
<institution>Royal Holloway University of London,</institution>
</institution-wrap>
Egham, UK</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Egham</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Walling, Linda L" sort="Walling, Linda L" uniqKey="Walling L" first="Linda L." last="Walling">Linda L. Walling</name>
<affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Californie</region>
</placeName>
<wicri:cityArea>Riverside</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC Genomics</title>
<idno type="eISSN">1471-2164</idno>
<imprint><date when="2020">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p id="Par1">Whiteflies are a threat to cassava (<italic>Manihot esculenta</italic>
), an important staple food in many tropical/subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein families are an integral part of plant immunity. With the availability of whole genome sequences, the annotation and expression programs of the full complement of <italic>PR</italic>
genes in an organism can now be achieved. An understanding of the responses of the entire complement of <italic>PR</italic>
genes during biotic stress and to the defense hormones, salicylic acid (SA) and jasmonic acid (JA), is lacking. Here, we analyze the responses of cassava <italic>PR</italic>
genes to whiteflies, SA, JA, and other biotic aggressors.</p>
</sec>
<sec><title>Results</title>
<p id="Par2">The cassava genome possesses 14 of the 17 plant <italic>PR</italic>
families, with a total of 447 <italic>PR</italic>
genes. A cassava <italic>PR</italic>
gene nomenclature is proposed. Phylogenetic relatedness of cassava PR proteins to each other and to homologs in poplar, rice and Arabidopsis identified cassava-specific <italic>PR</italic>
gene family expansions. The temporal programs of <italic>PR</italic>
gene expression in response to the whitefly (<italic>Aleurotrachelus socialis</italic>
) in four whitefly-susceptible cassava genotypes showed that 167 of the 447 <italic>PR</italic>
genes were regulated after whitefly infestation. While the timing of <italic>PR</italic>
gene expression varied, over 37% of whitefly-regulated <italic>PR</italic>
genes were downregulated in all four genotypes. Notably, whitefly-responsive <italic>PR</italic>
genes were largely coordinately regulated by SA and JA. The analysis of cassava <italic>PR</italic>
gene expression in response to five other biotic stresses revealed a strong positive correlation between whitefly and <italic>Xanthomonas axonopodis</italic>
and <italic>Cassava Brown Streak Virus</italic>
responses and negative correlations between whitefly and <italic>Cassava Mosaic Virus</italic>
responses<italic>.</italic>
Finally, certain associations between <italic>PR</italic>
genes in cassava expansions and response to biotic stresses were observed among <italic>PR</italic>
families.</p>
</sec>
<sec><title>Conclusions</title>
<p id="Par3">This study represents the first genome-wide characterization of <italic>PR</italic>
genes in cassava. <italic>PR</italic>
gene responses to six biotic stresses and to SA and JA are demonstrably different to other angiosperms. We propose that our approach could be applied in other species to fully understand <italic>PR</italic>
gene regulation by pathogens, pests and the canonical defense hormones SA and JA.</p>
</sec>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Ceballos, H" uniqKey="Ceballos H">H Ceballos</name>
</author>
<author><name sortKey="Ramirez, J" uniqKey="Ramirez J">J Ramirez</name>
</author>
<author><name sortKey="Bellotti, Ac" uniqKey="Bellotti A">AC Bellotti</name>
</author>
<author><name sortKey="Jarvis, A" uniqKey="Jarvis A">A Jarvis</name>
</author>
<author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jarvis, A" uniqKey="Jarvis A">A Jarvis</name>
</author>
<author><name sortKey="Ramirez Villegas, J" uniqKey="Ramirez Villegas J">J Ramirez-Villegas</name>
</author>
<author><name sortKey="Herrera Campo, Bv" uniqKey="Herrera Campo B">BV Herrera Campo</name>
</author>
<author><name sortKey="Navarro Racines, C" uniqKey="Navarro Racines C">C Navarro-Racines</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ravi, V" uniqKey="Ravi V">V Ravi</name>
</author>
<author><name sortKey="Saravan, R" uniqKey="Saravan R">R Saravan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bellotti, A C" uniqKey="Bellotti A">A.C Bellotti</name>
</author>
<author><name sortKey="Arias, B" uniqKey="Arias B">B Arias</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lundgren, Jg" uniqKey="Lundgren J">JG Lundgren</name>
</author>
<author><name sortKey="L Pez Lavalle, Lab" uniqKey="L Pez Lavalle L">LAB López-Lavalle</name>
</author>
<author><name sortKey="Parsa, S" uniqKey="Parsa S">S Parsa</name>
</author>
<author><name sortKey="Wyckhuys, Kag" uniqKey="Wyckhuys K">KAG Wyckhuys</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bellotti, Ac" uniqKey="Bellotti A">AC Bellotti</name>
</author>
<author><name sortKey="Smith, L" uniqKey="Smith L">L Smith</name>
</author>
<author><name sortKey="Lapointe, Sl" uniqKey="Lapointe S">SL Lapointe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alicai, T" uniqKey="Alicai T">T Alicai</name>
</author>
<author><name sortKey="Omongo, Ca" uniqKey="Omongo C">CA Omongo</name>
</author>
<author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Hillocks, Rj" uniqKey="Hillocks R">RJ Hillocks</name>
</author>
<author><name sortKey="Baguma, Y" uniqKey="Baguma Y">Y Baguma</name>
</author>
<author><name sortKey="Kawuki, R" uniqKey="Kawuki R">R Kawuki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Legg, Jp" uniqKey="Legg J">JP Legg</name>
</author>
<author><name sortKey="Ogwal, S" uniqKey="Ogwal S">S Ogwal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Legg, Jp" uniqKey="Legg J">JP Legg</name>
</author>
<author><name sortKey="Sseruwagi, P" uniqKey="Sseruwagi P">P Sseruwagi</name>
</author>
<author><name sortKey="Boniface, S" uniqKey="Boniface S">S Boniface</name>
</author>
<author><name sortKey="Okao Okuja, G" uniqKey="Okao Okuja G">G Okao-Okuja</name>
</author>
<author><name sortKey="Shirima, R" uniqKey="Shirima R">R Shirima</name>
</author>
<author><name sortKey="Bigirimana, S" uniqKey="Bigirimana S">S Bigirimana</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Colvin, J" uniqKey="Colvin J">J Colvin</name>
</author>
<author><name sortKey="Omongo, Ca" uniqKey="Omongo C">CA Omongo</name>
</author>
<author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Otim Nape, Gw" uniqKey="Otim Nape G">GW Otim-Nape</name>
</author>
<author><name sortKey="Thresh, Jm" uniqKey="Thresh J">JM Thresh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fauquet, C" uniqKey="Fauquet C">C Fauquet</name>
</author>
<author><name sortKey="Fargette, D" uniqKey="Fargette D">D Fargette</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hillocks, Rj" uniqKey="Hillocks R">RJ Hillocks</name>
</author>
<author><name sortKey="Raya, Md" uniqKey="Raya M">MD Raya</name>
</author>
<author><name sortKey="Mtunda, K" uniqKey="Mtunda K">K Mtunda</name>
</author>
<author><name sortKey="Kiozia, H" uniqKey="Kiozia H">H Kiozia</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Macfadyen, S" uniqKey="Macfadyen S">S Macfadyen</name>
</author>
<author><name sortKey="Paull, C" uniqKey="Paull C">C Paull</name>
</author>
<author><name sortKey="Boykin, Lm" uniqKey="Boykin L">LM Boykin</name>
</author>
<author><name sortKey="De Barro, P" uniqKey="De Barro P">P De Barro</name>
</author>
<author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Otim, M" uniqKey="Otim M">M Otim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jones, Jd" uniqKey="Jones J">JD Jones</name>
</author>
<author><name sortKey="Dangl, Jl" uniqKey="Dangl J">JL Dangl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Monaghan, J" uniqKey="Monaghan J">J Monaghan</name>
</author>
<author><name sortKey="Zipfel, C" uniqKey="Zipfel C">C Zipfel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Duran Flores, D" uniqKey="Duran Flores D">D Duran-Flores</name>
</author>
<author><name sortKey="Heil, M" uniqKey="Heil M">M Heil</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cui, H" uniqKey="Cui H">H Cui</name>
</author>
<author><name sortKey="Tsuda, K" uniqKey="Tsuda K">K Tsuda</name>
</author>
<author><name sortKey="Parker, Je" uniqKey="Parker J">JE Parker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Loon, Lc" uniqKey="Van Loon L">LC van Loon</name>
</author>
<author><name sortKey="Van Kammen, A" uniqKey="Van Kammen A">A van Kammen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hou, M" uniqKey="Hou M">M Hou</name>
</author>
<author><name sortKey="Xu, W" uniqKey="Xu W">W Xu</name>
</author>
<author><name sortKey="Bai, H" uniqKey="Bai H">H Bai</name>
</author>
<author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name>
</author>
<author><name sortKey="Li, L" uniqKey="Li L">L Li</name>
</author>
<author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Loon, Lc" uniqKey="Van Loon L">LC van Loon</name>
</author>
<author><name sortKey="Rep, M" uniqKey="Rep M">M Rep</name>
</author>
<author><name sortKey="Pieterse, Cm" uniqKey="Pieterse C">CM Pieterse</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fister, As" uniqKey="Fister A">AS Fister</name>
</author>
<author><name sortKey="Mejia, Lc" uniqKey="Mejia L">LC Mejia</name>
</author>
<author><name sortKey="Zhang, Y" uniqKey="Zhang Y">Y Zhang</name>
</author>
<author><name sortKey="Herre, Ea" uniqKey="Herre E">EA Herre</name>
</author>
<author><name sortKey="Maximova, Sn" uniqKey="Maximova S">SN Maximova</name>
</author>
<author><name sortKey="Guiltinan, Mj" uniqKey="Guiltinan M">MJ Guiltinan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Visser, Ea" uniqKey="Visser E">EA Visser</name>
</author>
<author><name sortKey="Wegrzyn, Jl" uniqKey="Wegrzyn J">JL Wegrzyn</name>
</author>
<author><name sortKey="Myburg, Aa" uniqKey="Myburg A">AA Myburg</name>
</author>
<author><name sortKey="Naidoo, S" uniqKey="Naidoo S">S Naidoo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wanderley Nogueira, Ac" uniqKey="Wanderley Nogueira A">AC Wanderley-Nogueira</name>
</author>
<author><name sortKey="Belarmino, Lc" uniqKey="Belarmino L">LC Belarmino</name>
</author>
<author><name sortKey="Soares Cavalcanti, Nm" uniqKey="Soares Cavalcanti N">NM Soares-Cavalcanti</name>
</author>
<author><name sortKey="Bezerra Neto, Jp" uniqKey="Bezerra Neto J">JP Bezerra-Neto</name>
</author>
<author><name sortKey="Kido, Ea" uniqKey="Kido E">EA Kido</name>
</author>
<author><name sortKey="Pandolfi, V" uniqKey="Pandolfi V">V Pandolfi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S Duplessis</name>
</author>
<author><name sortKey="Major, I" uniqKey="Major I">I Major</name>
</author>
<author><name sortKey="Martin, F" uniqKey="Martin F">F Martin</name>
</author>
<author><name sortKey="Seguin, A" uniqKey="Seguin A">A Séguin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rauwane, Me" uniqKey="Rauwane M">ME Rauwane</name>
</author>
<author><name sortKey="Odeny, Da" uniqKey="Odeny D">DA Odeny</name>
</author>
<author><name sortKey="Millar, I" uniqKey="Millar I">I Millar</name>
</author>
<author><name sortKey="Rey, C" uniqKey="Rey C">C Rey</name>
</author>
<author><name sortKey="Rees, J" uniqKey="Rees J">J Rees</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lopez, C" uniqKey="Lopez C">C Lopez</name>
</author>
<author><name sortKey="Soto, M" uniqKey="Soto M">M Soto</name>
</author>
<author><name sortKey="Restrepo, S" uniqKey="Restrepo S">S Restrepo</name>
</author>
<author><name sortKey="Piegu, B" uniqKey="Piegu B">B Piegu</name>
</author>
<author><name sortKey="Cooke, R" uniqKey="Cooke R">R Cooke</name>
</author>
<author><name sortKey="Delseny, M" uniqKey="Delseny M">M Delseny</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mu Oz Bodnar, A" uniqKey="Mu Oz Bodnar A">A Muñoz-Bodnar</name>
</author>
<author><name sortKey="Perez Quintero, A" uniqKey="Perez Quintero A">A Perez-Quintero</name>
</author>
<author><name sortKey="Gomez Cano, F" uniqKey="Gomez Cano F">F Gomez-Cano</name>
</author>
<author><name sortKey="Gil, J" uniqKey="Gil J">J Gil</name>
</author>
<author><name sortKey="Michelmore, R" uniqKey="Michelmore R">R Michelmore</name>
</author>
<author><name sortKey="Bernal, A" uniqKey="Bernal A">A Bernal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Utsumi, Y" uniqKey="Utsumi Y">Y Utsumi</name>
</author>
<author><name sortKey="Tanaka, M" uniqKey="Tanaka M">M Tanaka</name>
</author>
<author><name sortKey="Kurotani, A" uniqKey="Kurotani A">A Kurotani</name>
</author>
<author><name sortKey="Yoshida, T" uniqKey="Yoshida T">T Yoshida</name>
</author>
<author><name sortKey="Mochida, K" uniqKey="Mochida K">K Mochida</name>
</author>
<author><name sortKey="Matsui, A" uniqKey="Matsui A">A Matsui</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Allie, F" uniqKey="Allie F">F Allie</name>
</author>
<author><name sortKey="Pierce, Ej" uniqKey="Pierce E">EJ Pierce</name>
</author>
<author><name sortKey="Okoniewski, Mj" uniqKey="Okoniewski M">MJ Okoniewski</name>
</author>
<author><name sortKey="Rey, C" uniqKey="Rey C">C Rey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Amuge, T" uniqKey="Amuge T">T Amuge</name>
</author>
<author><name sortKey="Berger, Dk" uniqKey="Berger D">DK Berger</name>
</author>
<author><name sortKey="Katari, Ms" uniqKey="Katari M">MS Katari</name>
</author>
<author><name sortKey="Myburg, Aa" uniqKey="Myburg A">AA Myburg</name>
</author>
<author><name sortKey="Goldman, Sl" uniqKey="Goldman S">SL Goldman</name>
</author>
<author><name sortKey="Ferguson, Me" uniqKey="Ferguson M">ME Ferguson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Bouvaine, S" uniqKey="Bouvaine S">S Bouvaine</name>
</author>
<author><name sortKey="Tufan, Ha" uniqKey="Tufan H">HA Tufan</name>
</author>
<author><name sortKey="Mohammed, Iu" uniqKey="Mohammed I">IU Mohammed</name>
</author>
<author><name sortKey="Hillocks, Rj" uniqKey="Hillocks R">RJ Hillocks</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Mur, La" uniqKey="Mur L">LA Mur</name>
</author>
<author><name sortKey="Kenton, P" uniqKey="Kenton P">P Kenton</name>
</author>
<author><name sortKey="Atzorn, R" uniqKey="Atzorn R">R Atzorn</name>
</author>
<author><name sortKey="Miersch, O" uniqKey="Miersch O">O Miersch</name>
</author>
<author><name sortKey="Wasternack, C" uniqKey="Wasternack C">C Wasternack</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Robert Seilaniantz, A" uniqKey="Robert Seilaniantz A">A Robert-Seilaniantz</name>
</author>
<author><name sortKey="Grant, M" uniqKey="Grant M">M Grant</name>
</author>
<author><name sortKey="Jones, Jd" uniqKey="Jones J">JD Jones</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stec, B" uniqKey="Stec B">B Stec</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Silverstein, Ka" uniqKey="Silverstein K">KA Silverstein</name>
</author>
<author><name sortKey="Graham, Ma" uniqKey="Graham M">MA Graham</name>
</author>
<author><name sortKey="Paape, Td" uniqKey="Paape T">TD Paape</name>
</author>
<author><name sortKey="Vandenbosch, Ka" uniqKey="Vandenbosch K">KA VandenBosch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jorda, L" uniqKey="Jorda L">L Jorda</name>
</author>
<author><name sortKey="Coego, A" uniqKey="Coego A">A Coego</name>
</author>
<author><name sortKey="Conejero, V" uniqKey="Conejero V">V Conejero</name>
</author>
<author><name sortKey="Vera, P" uniqKey="Vera P">P Vera</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lebel, S" uniqKey="Lebel S">S Lebel</name>
</author>
<author><name sortKey="Schellenbaum, P" uniqKey="Schellenbaum P">P Schellenbaum</name>
</author>
<author><name sortKey="Walter, B" uniqKey="Walter B">B Walter</name>
</author>
<author><name sortKey="Maillot, P" uniqKey="Maillot P">P Maillot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jacob, F" uniqKey="Jacob F">F Jacob</name>
</author>
<author><name sortKey="Vernaldi, S" uniqKey="Vernaldi S">S Vernaldi</name>
</author>
<author><name sortKey="Maekawa, T" uniqKey="Maekawa T">T Maekawa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Yin, G" uniqKey="Yin G">G Yin</name>
</author>
<author><name sortKey="Xu, H" uniqKey="Xu H">H Xu</name>
</author>
<author><name sortKey="Xiao, S" uniqKey="Xiao S">S Xiao</name>
</author>
<author><name sortKey="Qin, Y" uniqKey="Qin Y">Y Qin</name>
</author>
<author><name sortKey="Li, Y" uniqKey="Li Y">Y Li</name>
</author>
<author><name sortKey="Yan, Y" uniqKey="Yan Y">Y Yan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gupta, P" uniqKey="Gupta P">P Gupta</name>
</author>
<author><name sortKey="Ravi, I" uniqKey="Ravi I">I Ravi</name>
</author>
<author><name sortKey="Sharma, V" uniqKey="Sharma V">V Sharma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sridevi, G" uniqKey="Sridevi G">G Sridevi</name>
</author>
<author><name sortKey="Parameswari, C" uniqKey="Parameswari C">C Parameswari</name>
</author>
<author><name sortKey="Sabapathi, N" uniqKey="Sabapathi N">N Sabapathi</name>
</author>
<author><name sortKey="Raghupathy, V" uniqKey="Raghupathy V">V Raghupathy</name>
</author>
<author><name sortKey="Veluthambi, K" uniqKey="Veluthambi K">K Veluthambi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jongedijk, E" uniqKey="Jongedijk E">E Jongedijk</name>
</author>
<author><name sortKey="Tigelaar, H" uniqKey="Tigelaar H">H Tigelaar</name>
</author>
<author><name sortKey="Van Roekel, Jsc" uniqKey="Van Roekel J">JSC van Roekel</name>
</author>
<author><name sortKey="Bres Vloemans, Sa" uniqKey="Bres Vloemans S">SA Bres-Vloemans</name>
</author>
<author><name sortKey="Dekker, I" uniqKey="Dekker I">I Dekker</name>
</author>
<author><name sortKey="Van Den Elzen, Pjm" uniqKey="Van Den Elzen P">PJM van den Elzen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kaloshian, I" uniqKey="Kaloshian I">I Kaloshian</name>
</author>
<author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, X W" uniqKey="Wang X">X-W Wang</name>
</author>
<author><name sortKey="Li, P" uniqKey="Li P">P Li</name>
</author>
<author><name sortKey="Shu Sheng, L" uniqKey="Shu Sheng L">L Shu-Sheng</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Antony, B" uniqKey="Antony B">B Antony</name>
</author>
<author><name sortKey="Palaniswami, Ms" uniqKey="Palaniswami M">MS Palaniswami</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Roman, V" uniqKey="Roman V">V Román</name>
</author>
<author><name sortKey="Bossa Castro, Am" uniqKey="Bossa Castro A">AM Bossa-Castro</name>
</author>
<author><name sortKey="Vasquez, A" uniqKey="Vasquez A">A Vásquez</name>
</author>
<author><name sortKey="Bernal, V" uniqKey="Bernal V">V Bernal</name>
</author>
<author><name sortKey="Schuster, M" uniqKey="Schuster M">M Schuster</name>
</author>
<author><name sortKey="Bernal, N" uniqKey="Bernal N">N Bernal</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, Jh" uniqKey="Lee J">JH Lee</name>
</author>
<author><name sortKey="Takei, K" uniqKey="Takei K">K Takei</name>
</author>
<author><name sortKey="Sakakibara, H" uniqKey="Sakakibara H">H Sakakibara</name>
</author>
<author><name sortKey="Sun Cho, H" uniqKey="Sun Cho H">H Sun Cho</name>
</author>
<author><name sortKey="Kim, Dm" uniqKey="Kim D">DM Kim</name>
</author>
<author><name sortKey="Kim, Ys" uniqKey="Kim Y">YS Kim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kragh, Km" uniqKey="Kragh K">KM Kragh</name>
</author>
<author><name sortKey="Hendriks, T" uniqKey="Hendriks T">T Hendriks</name>
</author>
<author><name sortKey="De Jong, Aj" uniqKey="De Jong A">AJ de Jong</name>
</author>
<author><name sortKey="Lo Schiavo, F" uniqKey="Lo Schiavo F">F Lo Schiavo</name>
</author>
<author><name sortKey="Bucherna, N" uniqKey="Bucherna N">N Bucherna</name>
</author>
<author><name sortKey="Hojrup, P" uniqKey="Hojrup P">P Hojrup</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kempema, La" uniqKey="Kempema L">LA Kempema</name>
</author>
<author><name sortKey="Cui, X" uniqKey="Cui X">X Cui</name>
</author>
<author><name sortKey="Holzer, Fm" uniqKey="Holzer F">FM Holzer</name>
</author>
<author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zarate, Si" uniqKey="Zarate S">SI Zarate</name>
</author>
<author><name sortKey="Kempema, La" uniqKey="Kempema L">LA Kempema</name>
</author>
<author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Glazebrook, J" uniqKey="Glazebrook J">J Glazebrook</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Park, Ys" uniqKey="Park Y">YS Park</name>
</author>
<author><name sortKey="Ryu, Cm" uniqKey="Ryu C">CM Ryu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pieterse, Cmj" uniqKey="Pieterse C">CMJ Pieterse</name>
</author>
<author><name sortKey="Leon Reyes, A" uniqKey="Leon Reyes A">A Leon-reyes</name>
</author>
<author><name sortKey="Van Der Ent, S" uniqKey="Van Der Ent S">S Van Der Ent</name>
</author>
<author><name sortKey="Van Wees, Scm" uniqKey="Van Wees S">SCM Van Wees</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schenk, Pm" uniqKey="Schenk P">PM Schenk</name>
</author>
<author><name sortKey="Kazan, K" uniqKey="Kazan K">K Kazan</name>
</author>
<author><name sortKey="Wilson, L" uniqKey="Wilson L">L Wilson</name>
</author>
<author><name sortKey="Anderson, Jp" uniqKey="Anderson J">JP Anderson</name>
</author>
<author><name sortKey="Richmond, T" uniqKey="Richmond T">T Richmond</name>
</author>
<author><name sortKey="Somerville, Sc" uniqKey="Somerville S">SC Somerville</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tamaoki, D" uniqKey="Tamaoki D">D Tamaoki</name>
</author>
<author><name sortKey="Seo, S" uniqKey="Seo S">S Seo</name>
</author>
<author><name sortKey="Yamada, S" uniqKey="Yamada S">S Yamada</name>
</author>
<author><name sortKey="Kano, A" uniqKey="Kano A">A Kano</name>
</author>
<author><name sortKey="Miyamoto, A" uniqKey="Miyamoto A">A Miyamoto</name>
</author>
<author><name sortKey="Shishido, H" uniqKey="Shishido H">H Shishido</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zhu Salzman, K" uniqKey="Zhu Salzman K">K Zhu-Salzman</name>
</author>
<author><name sortKey="Salzman, Ra" uniqKey="Salzman R">RA Salzman</name>
</author>
<author><name sortKey="Ahn, Je" uniqKey="Ahn J">JE Ahn</name>
</author>
<author><name sortKey="Koiwa, H" uniqKey="Koiwa H">H Koiwa</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Abad, Lr" uniqKey="Abad L">LR Abad</name>
</author>
<author><name sortKey="D Urzo, Mp" uniqKey="D Urzo M">MP D'Urzo</name>
</author>
<author><name sortKey="Liu, D" uniqKey="Liu D">D Liu</name>
</author>
<author><name sortKey="Narasimhan, Ml" uniqKey="Narasimhan M">ML Narasimhan</name>
</author>
<author><name sortKey="Reuveni, M" uniqKey="Reuveni M">M Reuveni</name>
</author>
<author><name sortKey="Zhu, Jk" uniqKey="Zhu J">JK Zhu</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Lagrimini, Lm" uniqKey="Lagrimini L">LM Lagrimini</name>
</author>
<author><name sortKey="Burkhart, W" uniqKey="Burkhart W">W Burkhart</name>
</author>
<author><name sortKey="Moyer, M" uniqKey="Moyer M">M Moyer</name>
</author>
<author><name sortKey="Rothstein, S" uniqKey="Rothstein S">S Rothstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Foyer, Ch" uniqKey="Foyer C">CH Foyer</name>
</author>
<author><name sortKey="Verrall, Sr" uniqKey="Verrall S">SR Verrall</name>
</author>
<author><name sortKey="Hancock, Rd" uniqKey="Hancock R">RD Hancock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Puthoff, Dp" uniqKey="Puthoff D">DP Puthoff</name>
</author>
<author><name sortKey="Holzer, Fm" uniqKey="Holzer F">FM Holzer</name>
</author>
<author><name sortKey="Perring, Tm" uniqKey="Perring T">TM Perring</name>
</author>
<author><name sortKey="Walling, Ll" uniqKey="Walling L">LL Walling</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Quintana Camargo, M" uniqKey="Quintana Camargo M">M Quintana-Camargo</name>
</author>
<author><name sortKey="Mendez Moran, L" uniqKey="Mendez Moran L">L Mendez-Moran</name>
</author>
<author><name sortKey="Ramirez Romero, R" uniqKey="Ramirez Romero R">R Ramirez-Romero</name>
</author>
<author><name sortKey="Gurrola Diaz, Cm" uniqKey="Gurrola Diaz C">CM Gurrola-Diaz</name>
</author>
<author><name sortKey="Carapia Ruiz, V" uniqKey="Carapia Ruiz V">V Carapia-Ruiz</name>
</author>
<author><name sortKey="Ibarra Laclette, E" uniqKey="Ibarra Laclette E">E Ibarra-Laclette</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Moran, Pj" uniqKey="Moran P">PJ Moran</name>
</author>
<author><name sortKey="Cheng, Y" uniqKey="Cheng Y">Y Cheng</name>
</author>
<author><name sortKey="Cassell, Jl" uniqKey="Cassell J">JL Cassell</name>
</author>
<author><name sortKey="Thompson, Ga" uniqKey="Thompson G">GA Thompson</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ng, Jck" uniqKey="Ng J">JCK Ng</name>
</author>
<author><name sortKey="Falk, Bw" uniqKey="Falk B">BW Falk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Colvin, J" uniqKey="Colvin J">J Colvin</name>
</author>
<author><name sortKey="Seal, S" uniqKey="Seal S">S Seal</name>
</author>
<author><name sortKey="Thresh, Jm" uniqKey="Thresh J">JM Thresh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maruthi, Mn" uniqKey="Maruthi M">MN Maruthi</name>
</author>
<author><name sortKey="Jeremiah, Sc" uniqKey="Jeremiah S">SC Jeremiah</name>
</author>
<author><name sortKey="Mohammed, Iu" uniqKey="Mohammed I">IU Mohammed</name>
</author>
<author><name sortKey="Legg, Jp" uniqKey="Legg J">JP Legg</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parsa, S" uniqKey="Parsa S">S Parsa</name>
</author>
<author><name sortKey="Medina, C" uniqKey="Medina C">C Medina</name>
</author>
<author><name sortKey="Rodriguez, V" uniqKey="Rodriguez V">V Rodríguez</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bohorquez, A" uniqKey="Bohorquez A">A Bohorquez</name>
</author>
<author><name sortKey="Tohme, J" uniqKey="Tohme J">J Tohme</name>
</author>
<author><name sortKey="Parsa, S" uniqKey="Parsa S">S Parsa</name>
</author>
<author><name sortKey="Becerra L Pez Lavalle, La" uniqKey="Becerra L Pez Lavalle L">LA Becerra López-Lavalle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Escobar Bravo, R" uniqKey="Escobar Bravo R">R Escobar-Bravo</name>
</author>
<author><name sortKey="Alba, Jm" uniqKey="Alba J">JM Alba</name>
</author>
<author><name sortKey="Pons, C" uniqKey="Pons C">C Pons</name>
</author>
<author><name sortKey="Granell, A" uniqKey="Granell A">A Granell</name>
</author>
<author><name sortKey="Kant, Mr" uniqKey="Kant M">MR Kant</name>
</author>
<author><name sortKey="Moriones, E" uniqKey="Moriones E">E Moriones</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mandal, Sudhamoy" uniqKey="Mandal S">Sudhamoy Mandal</name>
</author>
<author><name sortKey="Mallick, Nirupama" uniqKey="Mallick N">Nirupama Mallick</name>
</author>
<author><name sortKey="Mitra, Adinpunya" uniqKey="Mitra A">Adinpunya Mitra</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Finn, Rd" uniqKey="Finn R">RD Finn</name>
</author>
<author><name sortKey="Clements, J" uniqKey="Clements J">J Clements</name>
</author>
<author><name sortKey="Eddy, Sr" uniqKey="Eddy S">SR Eddy</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Finn, Rd" uniqKey="Finn R">RD Finn</name>
</author>
<author><name sortKey="Coggill, P" uniqKey="Coggill P">P Coggill</name>
</author>
<author><name sortKey="Eberhardt, Ry" uniqKey="Eberhardt R">RY Eberhardt</name>
</author>
<author><name sortKey="Eddy, Sr" uniqKey="Eddy S">SR Eddy</name>
</author>
<author><name sortKey="Mistry, J" uniqKey="Mistry J">J Mistry</name>
</author>
<author><name sortKey="Mitchell, Al" uniqKey="Mitchell A">AL Mitchell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kearse, M" uniqKey="Kearse M">M Kearse</name>
</author>
<author><name sortKey="Moir, R" uniqKey="Moir R">R Moir</name>
</author>
<author><name sortKey="Wilson, A" uniqKey="Wilson A">A Wilson</name>
</author>
<author><name sortKey="Stones Havas, S" uniqKey="Stones Havas S">S Stones-Havas</name>
</author>
<author><name sortKey="Cheung, M" uniqKey="Cheung M">M Cheung</name>
</author>
<author><name sortKey="Sturrock, S" uniqKey="Sturrock S">S Sturrock</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wolfe, D" uniqKey="Wolfe D">D Wolfe</name>
</author>
<author><name sortKey="Dudek, S" uniqKey="Dudek S">S Dudek</name>
</author>
<author><name sortKey="Ritchie, Md" uniqKey="Ritchie M">MD Ritchie</name>
</author>
<author><name sortKey="Pendergrass, Sa" uniqKey="Pendergrass S">SA Pendergrass</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Behnam, B" uniqKey="Behnam B">B Behnam</name>
</author>
<author><name sortKey="Bohorquez Chaux, A" uniqKey="Bohorquez Chaux A">A Bohorquez-Chaux</name>
</author>
<author><name sortKey="Castaneda Mendez, Of" uniqKey="Castaneda Mendez O">OF Castaneda-Mendez</name>
</author>
<author><name sortKey="Tsuji, H" uniqKey="Tsuji H">H Tsuji</name>
</author>
<author><name sortKey="Ishitani, M" uniqKey="Ishitani M">M Ishitani</name>
</author>
<author><name sortKey="Becerra Lopez Lavalle, La" uniqKey="Becerra Lopez Lavalle L">LA Becerra Lopez-Lavalle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, L" uniqKey="Wang L">L Wang</name>
</author>
<author><name sortKey="Si, Y" uniqKey="Si Y">Y Si</name>
</author>
<author><name sortKey="Dedow, L" uniqKey="Dedow L">L Dedow</name>
</author>
<author><name sortKey="Shao, Y" uniqKey="Shao Y">Y Shao</name>
</author>
<author><name sortKey="Liu, P" uniqKey="Liu P">P Liu</name>
</author>
<author><name sortKey="Brutnell, T" uniqKey="Brutnell T">T Brutnell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Goodstein, Dm" uniqKey="Goodstein D">DM Goodstein</name>
</author>
<author><name sortKey="Shu, S" uniqKey="Shu S">S Shu</name>
</author>
<author><name sortKey="Howson, R" uniqKey="Howson R">R Howson</name>
</author>
<author><name sortKey="Neupane, R" uniqKey="Neupane R">R Neupane</name>
</author>
<author><name sortKey="Hayes, Rd" uniqKey="Hayes R">RD Hayes</name>
</author>
<author><name sortKey="Fazo, J" uniqKey="Fazo J">J Fazo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Backman, Twh" uniqKey="Backman T">TWH Backman</name>
</author>
<author><name sortKey="Girke, T" uniqKey="Girke T">T Girke</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gu, Z" uniqKey="Gu Z">Z Gu</name>
</author>
<author><name sortKey="Eils, R" uniqKey="Eils R">R Eils</name>
</author>
<author><name sortKey="Schlesner, M" uniqKey="Schlesner M">M Schlesner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oliveros, Jc" uniqKey="Oliveros J">JC Oliveros</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wilson, Mc" uniqKey="Wilson M">MC Wilson</name>
</author>
<author><name sortKey="Mutka, Am" uniqKey="Mutka A">AM Mutka</name>
</author>
<author><name sortKey="Hummel, Aw" uniqKey="Hummel A">AW Hummel</name>
</author>
<author><name sortKey="Berry, J" uniqKey="Berry J">J Berry</name>
</author>
<author><name sortKey="Chauhan, Rd" uniqKey="Chauhan R">RD Chauhan</name>
</author>
<author><name sortKey="Vijayaraghavan, A" uniqKey="Vijayaraghavan A">A Vijayaraghavan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Livak, Kenneth J" uniqKey="Livak K">Kenneth J. Livak</name>
</author>
<author><name sortKey="Schmittgen, Thomas D" uniqKey="Schmittgen T">Thomas D. Schmittgen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wickman, H" uniqKey="Wickman H">H Wickman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Evans, Jd" uniqKey="Evans J">JD Evans</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Genomics</journal-id>
<journal-id journal-id-type="iso-abbrev">BMC Genomics</journal-id>
<journal-title-group><journal-title>BMC Genomics</journal-title>
</journal-title-group>
<issn pub-type="epub">1471-2164</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">31996126</article-id>
<article-id pub-id-type="pmc">6990599</article-id>
<article-id pub-id-type="publisher-id">6443</article-id>
<article-id pub-id-type="doi">10.1186/s12864-019-6443-1</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Genome-wide analyses of cassava <italic>Pathogenesis-related</italic>
(<italic>PR</italic>
) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Irigoyen</surname>
<given-names>Maria L.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Garceau</surname>
<given-names>Danielle C.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bohorquez-Chaux</surname>
<given-names>Adriana</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lopez-Lavalle</surname>
<given-names>Luis Augusto Becerra</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Perez-Fons</surname>
<given-names>Laura</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fraser</surname>
<given-names>Paul D.</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">http://orcid.org/0000-0003-3006-9220</contrib-id>
<name><surname>Walling</surname>
<given-names>Linda L.</given-names>
</name>
<address><email>lwalling@ucr.edu</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<aff id="Aff1"><label>1</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2222 1582</institution-id>
<institution-id institution-id-type="GRID">grid.266097.c</institution-id>
<institution>Department of Botany and Plant Sciences and Institute of Integrative Genome Biology,</institution>
<institution>University of California,</institution>
</institution-wrap>
Riverside, CA 92521 USA</aff>
<aff id="Aff2"><label>2</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0943 556X</institution-id>
<institution-id institution-id-type="GRID">grid.418348.2</institution-id>
<institution>International Center of Tropical Agriculture (CIAT),</institution>
</institution-wrap>
Cali, Colombia</aff>
<aff id="Aff3"><label>3</label>
<institution-wrap><institution-id institution-id-type="ISNI">0000 0001 2188 881X</institution-id>
<institution-id institution-id-type="GRID">grid.4970.a</institution-id>
<institution>Department of Biological Sciences,</institution>
<institution>Royal Holloway University of London,</institution>
</institution-wrap>
Egham, UK</aff>
</contrib-group>
<pub-date pub-type="epub"><day>29</day>
<month>1</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>29</day>
<month>1</month>
<year>2020</year>
</pub-date>
<pub-date pub-type="collection"><year>2020</year>
</pub-date>
<volume>21</volume>
<elocation-id>93</elocation-id>
<history><date date-type="received"><day>10</day>
<month>10</month>
<year>2019</year>
</date>
<date date-type="accepted"><day>29</day>
<month>12</month>
<year>2019</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s). 2020</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p id="Par1">Whiteflies are a threat to cassava (<italic>Manihot esculenta</italic>
), an important staple food in many tropical/subtropical regions. Understanding the molecular mechanisms regulating cassava’s responses against this pest is crucial for developing control strategies. Pathogenesis-related (PR) protein families are an integral part of plant immunity. With the availability of whole genome sequences, the annotation and expression programs of the full complement of <italic>PR</italic>
genes in an organism can now be achieved. An understanding of the responses of the entire complement of <italic>PR</italic>
genes during biotic stress and to the defense hormones, salicylic acid (SA) and jasmonic acid (JA), is lacking. Here, we analyze the responses of cassava <italic>PR</italic>
genes to whiteflies, SA, JA, and other biotic aggressors.</p>
</sec>
<sec><title>Results</title>
<p id="Par2">The cassava genome possesses 14 of the 17 plant <italic>PR</italic>
families, with a total of 447 <italic>PR</italic>
genes. A cassava <italic>PR</italic>
gene nomenclature is proposed. Phylogenetic relatedness of cassava PR proteins to each other and to homologs in poplar, rice and Arabidopsis identified cassava-specific <italic>PR</italic>
gene family expansions. The temporal programs of <italic>PR</italic>
gene expression in response to the whitefly (<italic>Aleurotrachelus socialis</italic>
) in four whitefly-susceptible cassava genotypes showed that 167 of the 447 <italic>PR</italic>
genes were regulated after whitefly infestation. While the timing of <italic>PR</italic>
gene expression varied, over 37% of whitefly-regulated <italic>PR</italic>
genes were downregulated in all four genotypes. Notably, whitefly-responsive <italic>PR</italic>
genes were largely coordinately regulated by SA and JA. The analysis of cassava <italic>PR</italic>
gene expression in response to five other biotic stresses revealed a strong positive correlation between whitefly and <italic>Xanthomonas axonopodis</italic>
and <italic>Cassava Brown Streak Virus</italic>
responses and negative correlations between whitefly and <italic>Cassava Mosaic Virus</italic>
responses<italic>.</italic>
Finally, certain associations between <italic>PR</italic>
genes in cassava expansions and response to biotic stresses were observed among <italic>PR</italic>
families.</p>
</sec>
<sec><title>Conclusions</title>
<p id="Par3">This study represents the first genome-wide characterization of <italic>PR</italic>
genes in cassava. <italic>PR</italic>
gene responses to six biotic stresses and to SA and JA are demonstrably different to other angiosperms. We propose that our approach could be applied in other species to fully understand <italic>PR</italic>
gene regulation by pathogens, pests and the canonical defense hormones SA and JA.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Cassava</kwd>
<kwd>Jasmonic acid</kwd>
<kwd>Pathogenesis-related</kwd>
<kwd><italic>PR</italic>
genes</kwd>
<kwd>PR proteins</kwd>
<kwd>Salicylic acid</kwd>
<kwd>Transcriptome</kwd>
<kwd>Whitefly</kwd>
<kwd>Stress response</kwd>
<kwd>Defense</kwd>
<kwd>Hormone</kwd>
<kwd>Pest</kwd>
</kwd-group>
<funding-group><award-group><funding-source><institution-wrap><institution-id institution-id-type="FundRef">http://dx.doi.org/10.13039/100000865</institution-id>
<institution>Bill and Melinda Gates Foundation</institution>
</institution-wrap>
</funding-source>
<award-id>Subcontract from the Natural Resources Institute, Greenwich, UK</award-id>
<award-id>Subcontract from National Resources Institute, Greenwich, UK</award-id>
<award-id>Subcontract from the Natural Resources Institute, Greenwich, UK</award-id>
<principal-award-recipient><name><surname>Lopez-Lavalle</surname>
<given-names>Luis Augusto Becerra</given-names>
</name>
<name><surname>Fraser</surname>
<given-names>Paul D.</given-names>
</name>
</principal-award-recipient>
</award-group>
</funding-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2020</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Colombie</li>
<li>Royaume-Uni</li>
<li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Californie"><name sortKey="Irigoyen, Maria L" sort="Irigoyen, Maria L" uniqKey="Irigoyen M" first="Maria L." last="Irigoyen">Maria L. Irigoyen</name>
</region>
<name sortKey="Garceau, Danielle C" sort="Garceau, Danielle C" uniqKey="Garceau D" first="Danielle C." last="Garceau">Danielle C. Garceau</name>
<name sortKey="Walling, Linda L" sort="Walling, Linda L" uniqKey="Walling L" first="Linda L." last="Walling">Linda L. Walling</name>
</country>
<country name="Colombie"><noRegion><name sortKey="Bohorquez Chaux, Adriana" sort="Bohorquez Chaux, Adriana" uniqKey="Bohorquez Chaux A" first="Adriana" last="Bohorquez-Chaux">Adriana Bohorquez-Chaux</name>
</noRegion>
<name sortKey="Lopez Lavalle, Luis Augusto Becerra" sort="Lopez Lavalle, Luis Augusto Becerra" uniqKey="Lopez Lavalle L" first="Luis Augusto Becerra" last="Lopez-Lavalle">Luis Augusto Becerra Lopez-Lavalle</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Perez Fons, Laura" sort="Perez Fons, Laura" uniqKey="Perez Fons L" first="Laura" last="Perez-Fons">Laura Perez-Fons</name>
</noRegion>
<name sortKey="Fraser, Paul D" sort="Fraser, Paul D" uniqKey="Fraser P" first="Paul D." last="Fraser">Paul D. Fraser</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000077 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000077 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= MelampsoraV2 |flux= Pmc |étape= Checkpoint |type= RBID |clé= PMC:6990599 |texte= Genome-wide analyses of cassava Pathogenesis-related (PR) gene families reveal core transcriptome responses to whitefly infestation, salicylic acid and jasmonic acid }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:31996126" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \ | NlmPubMed2Wicri -a MelampsoraV2
![]() | This area was generated with Dilib version V0.6.38. | ![]() |