Ultrastructure of hyphal cells of Trichophyton tonsurans
Identifieur interne : 000004 ( Pmc/Checkpoint ); précédent : 000003; suivant : 000005Ultrastructure of hyphal cells of Trichophyton tonsurans
Auteurs : Amaliya Stepanova [Russie] ; G. Sybren De Hoog [Pays-Bas] ; Nataliya Vasilyeva [Russie] ; Konstantin Raznatovskiy [Russie] ; Galina Chilina [Russie]Source :
- Current Medical Mycology [ 2423-3439 ] ; 2020.
Abstract
Differences in morphogenesis of aerial and substrate hyphae were revealed. Mitochondrial reticulum and fibrosinous bodies were shown in
Mature cells of substrate hyphae appeared more active than comparable cells in the aerial mycelium. During the maturation process, the differences in number and morphology of mitochondria, number of vacuoles, and in the synthesis of different types of storage substances were revealed. Presence of “mitochondrial reticulum” and variable types of storage substances in submerged hyphal cells suggested higher levels of metabolic activity compared to aerial mycelium.
Url:
DOI: 10.18502/cmm.6.1.2508
PubMed: 32420507
PubMed Central: 7217248
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:7217248Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ultrastructure of hyphal cells of <italic>Trichophyton tonsurans</italic></title><author><name sortKey="Stepanova, Amaliya" sort="Stepanova, Amaliya" uniqKey="Stepanova A" first="Amaliya" last="Stepanova">Amaliya Stepanova</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="De Hoog, G Sybren" sort="De Hoog, G Sybren" uniqKey="De Hoog G" first="G. Sybren" last="De Hoog">G. Sybren De Hoog</name><affiliation wicri:level="3"><nlm:aff id="aff2">Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Westerdijk Fungal Biodiversity Institute, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Centre of Expertise in Mycology of Radboud University Medical Centre / Canisius Wilhelmina Hospital, Nijmegen, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Centre of Expertise in Mycology of Radboud University Medical Centre / Canisius Wilhelmina Hospital, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Vasilyeva, Nataliya" sort="Vasilyeva, Nataliya" uniqKey="Vasilyeva N" first="Nataliya" last="Vasilyeva">Nataliya Vasilyeva</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Raznatovskiy, Konstantin" sort="Raznatovskiy, Konstantin" uniqKey="Raznatovskiy K" first="Konstantin" last="Raznatovskiy">Konstantin Raznatovskiy</name><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Dermatovenerology, North-Western State Medical University I.I. Mechnikov, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Dermatovenerology, North-Western State Medical University I.I. Mechnikov, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Chilina, Galina" sort="Chilina, Galina" uniqKey="Chilina G" first="Galina" last="Chilina">Galina Chilina</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">32420507</idno><idno type="pmc">7217248</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7217248</idno><idno type="RBID">PMC:7217248</idno><idno type="doi">10.18502/cmm.6.1.2508</idno><date when="2020">2020</date><idno type="wicri:Area/Pmc/Corpus">000608</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000608</idno><idno type="wicri:Area/Pmc/Curation">000608</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000608</idno><idno type="wicri:Area/Pmc/Checkpoint">000004</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000004</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Ultrastructure of hyphal cells of <italic>Trichophyton tonsurans</italic></title><author><name sortKey="Stepanova, Amaliya" sort="Stepanova, Amaliya" uniqKey="Stepanova A" first="Amaliya" last="Stepanova">Amaliya Stepanova</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="De Hoog, G Sybren" sort="De Hoog, G Sybren" uniqKey="De Hoog G" first="G. Sybren" last="De Hoog">G. Sybren De Hoog</name><affiliation wicri:level="3"><nlm:aff id="aff2">Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Westerdijk Fungal Biodiversity Institute, Utrecht</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2" type="province">Utrecht (province)</region></placeName></affiliation><affiliation wicri:level="3"><nlm:aff id="aff3">Centre of Expertise in Mycology of Radboud University Medical Centre / Canisius Wilhelmina Hospital, Nijmegen, The Netherlands</nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Centre of Expertise in Mycology of Radboud University Medical Centre / Canisius Wilhelmina Hospital, Nijmegen</wicri:regionArea><placeName><settlement type="city">Nimègue</settlement><region type="province" nuts="2">Gueldre</region></placeName></affiliation></author><author><name sortKey="Vasilyeva, Nataliya" sort="Vasilyeva, Nataliya" uniqKey="Vasilyeva N" first="Nataliya" last="Vasilyeva">Nataliya Vasilyeva</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Raznatovskiy, Konstantin" sort="Raznatovskiy, Konstantin" uniqKey="Raznatovskiy K" first="Konstantin" last="Raznatovskiy">Konstantin Raznatovskiy</name><affiliation wicri:level="1"><nlm:aff id="aff4">Department of Dermatovenerology, North-Western State Medical University I.I. Mechnikov, St. Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Department of Dermatovenerology, North-Western State Medical University I.I. Mechnikov, St. Petersburg</wicri:regionArea><wicri:noRegion>St. Petersburg</wicri:noRegion></affiliation></author><author><name sortKey="Chilina, Galina" sort="Chilina, Galina" uniqKey="Chilina G" first="Galina" last="Chilina">Galina Chilina</name><affiliation wicri:level="1"><nlm:aff id="aff1">North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg</wicri:regionArea><wicri:noRegion>Saint Petersburg</wicri:noRegion></affiliation></author></analytic><series><title level="j">Current Medical Mycology</title><idno type="ISSN">2423-3439</idno><idno type="eISSN">2423-3420</idno><imprint><date when="2020">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background and Purpose:</title><p><italic>Trichophyton tonsurans </italic>is a widely distributed anthropophilic dermatophyte causing different diseases of skin. In the literature limited data are available about the morphogenesis of vegetative mycelium of <italic>T. tonsurans</italic> and related anthropophilic dermatophytes. The aim of present study was to describe ultrastructural patterns of development, cellular organellography and septal pore apparatus structure of <italic>in vitro</italic> growing vegetative mycelium of <italic>T. tonsurans</italic>.</p></sec><sec><title>Materials and Methods:</title><p><italic>Trichophyton tonsurans</italic> strain RCPFF 214/898 was grown on solid Czapek’s Agar (CzA) at 28ºС. For investigation of colonies morphology we used methods of light-, scanning and transmission electron microscopy (SEM and TEM).</p></sec><sec><title>Results:</title><p>Differences in morphogenesis of aerial and substrate hyphae were revealed. Mitochondrial reticulum and fibrosinous bodies were shown in <italic>T. tonsurans</italic> for the first time. The septal pore apparatus in hyphal cells of was comprised Woronin bodies and septal pore plugs. Woronin bodies (0.18 µm), located with 1‒4 near the pore, were spherical, membrane-bound, and had a homogeneous, electron-dense content. The cells of aerial and submerged hyphal cells of <italic>T. tonsurans </italic>contain two nuclei.</p></sec><sec><title>Conclusion:</title><p>Mature cells of substrate hyphae appeared more active than comparable cells in the aerial mycelium. During the maturation process, the differences in number and morphology of mitochondria, number of vacuoles, and in the synthesis of different types of storage substances were revealed. Presence of “mitochondrial reticulum” and variable types of storage substances in submerged hyphal cells suggested higher levels of metabolic activity compared to aerial mycelium. </p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="De, Hoog Gs" uniqKey="De H">Hoog GS De</name></author><author><name sortKey="Guarro, J" uniqKey="Guarro J">J Guarro</name></author><author><name sortKey="Gene, J" uniqKey="Gene J">J Gené</name></author><author><name sortKey="Figueras, Mj" uniqKey="Figueras M">MJ Figueras</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gupta, Ak" uniqKey="Gupta A">AK Gupta</name></author><author><name sortKey="Mays, Rr" uniqKey="Mays R">RR Mays</name></author><author><name sortKey="Versteeg, Sg" uniqKey="Versteeg S">SG Versteeg</name></author><author><name sortKey="Piraccini, Bm" uniqKey="Piraccini B">BM Piraccini</name></author><author><name sortKey="Shear, Nm" uniqKey="Shear N">NM Shear</name></author><author><name sortKey="Piguet, V" uniqKey="Piguet V">V Piguet</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gupta, Ak" uniqKey="Gupta A">AK Gupta</name></author><author><name sortKey="Summerbell, Rc" uniqKey="Summerbell R">RC Summerbell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="El, Fari M" uniqKey="El F">Fari M el</name></author><author><name sortKey="Gr Ser, Y" uniqKey="Gr Ser Y">Y Gräser</name></author><author><name sortKey="Presber, W" uniqKey="Presber W">W Presber</name></author><author><name sortKey="Tietz, Hj" uniqKey="Tietz H">HJ Tietz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rasnatovskiy, Ki" uniqKey="Rasnatovskiy K">KI Rasnatovskiy</name></author><author><name sortKey="Rodionov, N" uniqKey="Rodionov ">АN Rodionov</name></author><author><name sortKey="Kotrechova, Lp" uniqKey="Kotrechova L">LP Kotrechova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kandemir, H" uniqKey="Kandemir H">H Kandemir</name></author><author><name sortKey="Dukik, K" uniqKey="Dukik K">K Dukik</name></author><author><name sortKey="Hagen, F" uniqKey="Hagen F">F Hagen</name></author><author><name sortKey="Ilkit, M" uniqKey="Ilkit M">M Ilkit</name></author><author><name sortKey="Gr Ser, Y" uniqKey="Gr Ser Y">Y Gräser</name></author><author><name sortKey="De, Hoog Gs" uniqKey="De H">Hoog GS de</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amer, Aa" uniqKey="Amer A">AA Amer</name></author><author><name sortKey="Tana, M" uniqKey="Tana M">M Tana</name></author><author><name sortKey="Diab, Na" uniqKey="Diab N">NA Diab</name></author><author><name sortKey="El, Moughith A" uniqKey="El M">Moughith A el</name></author><author><name sortKey="El, Harras M" uniqKey="El H">Harras M el</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Naka, W" uniqKey="Naka W">W Naka</name></author><author><name sortKey="Fukuda, T" uniqKey="Fukuda T">T Fukuda</name></author><author><name sortKey="Ohmi, T" uniqKey="Ohmi T">T Ohmi</name></author><author><name sortKey="Kanai, K" uniqKey="Kanai K">K Kanai</name></author><author><name sortKey="Nishikawa, T" uniqKey="Nishikawa T">T Nishikawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Savitskaya, Ti" uniqKey="Savitskaya T">TI Savitskaya</name></author><author><name sortKey="Vasilyeva" uniqKey="Vasilyeva">Vasilyeva</name></author><author><name sortKey="Nv" uniqKey="Nv">NV</name></author><author><name sortKey="Martynov, Aa" uniqKey="Martynov A">AA Martynov</name></author><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="Rasnatovskiy, Ki" uniqKey="Rasnatovskiy K">KI Rasnatovskiy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yue, X" uniqKey="Yue X">X Yue</name></author><author><name sortKey="Li, Q" uniqKey="Li Q">Q Li</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H Wang</name></author><author><name sortKey="Sun, Y" uniqKey="Sun Y">Y Sun</name></author><author><name sortKey="Wang, A" uniqKey="Wang A">A Wang</name></author><author><name sortKey="Zhang, Q" uniqKey="Zhang Q">Q Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pock Steen, B" uniqKey="Pock Steen B">B Pock-Steen</name></author><author><name sortKey="Kobayasi, T" uniqKey="Kobayasi T">T Kobayasi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey=", " uniqKey=" ">АА Степанова</name></author><author><name sortKey=", " uniqKey=" ">ИА Синицкая</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sagar, K" uniqKey="Sagar K">K Sagar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mochizuki, T" uniqKey="Mochizuki T">T Mochizuki</name></author><author><name sortKey="Anzawa, K" uniqKey="Anzawa K">K Anzawa</name></author><author><name sortKey="Sakata, Y" uniqKey="Sakata Y">Y Sakata</name></author><author><name sortKey="Fujihiro, M" uniqKey="Fujihiro M">M Fujihiro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey=", " uniqKey=" ">АЕ Васильев</name></author><author><name sortKey=", " uniqKey=" ">ФИ Камалетдинова</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harder, De" uniqKey="Harder D">DE Harder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hassan, Zm" uniqKey="Hassan Z">ZM Hassan</name></author><author><name sortKey="Littlefield, Lj" uniqKey="Littlefield L">LJ Littlefield</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey=", " uniqKey=" ">АА Степанова</name></author><author><name sortKey=", " uniqKey=" ">ИА Синицкая</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="Vasilyeva, Nv" uniqKey="Vasilyeva N">NV Vasilyeva</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Tong, D" uniqKey="Tong D">D Tong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Vasilyeva, Nv" uniqKey="Vasilyeva N">NV Vasilyeva</name></author><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="Sinitskaya, Ia" uniqKey="Sinitskaya I">IA Sinitskaya</name></author><author><name sortKey="Semenov, Vv" uniqKey="Semenov V">VV Semenov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="Vasilyeva, Nv" uniqKey="Vasilyeva N">NV Vasilyeva</name></author><author><name sortKey="Yamaguchi, M" uniqKey="Yamaguchi M">M Yamaguchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="Synitskaiya, Ia" uniqKey="Synitskaiya I">IA Synitskaiya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stepanova, Aa" uniqKey="Stepanova A">AA Stepanova</name></author><author><name sortKey="De, Hoog Gs" uniqKey="De H">Hoog GS de</name></author><author><name sortKey="Vasilyeva, Nv" uniqKey="Vasilyeva N">NV Vasilyeva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, Wh" uniqKey="Muller W">WH Muller</name></author><author><name sortKey="Montijn, Rc" uniqKey="Montijn R">RC Montijn</name></author><author><name sortKey="Humbel, Bm" uniqKey="Humbel B">BM Humbel</name></author><author><name sortKey="Van, Aelst Ac" uniqKey="Van A">Aelst AC van</name></author><author><name sortKey="Boon, Ejm" uniqKey="Boon E">EJM Boon</name></author><author><name sortKey="Van, Der Krift Tp" uniqKey="Van D">der Krift TP van</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Curr Med Mycol</journal-id><journal-id journal-id-type="iso-abbrev">Curr Med Mycol</journal-id><journal-id journal-id-type="publisher-id">CMM</journal-id><journal-title-group><journal-title>Current Medical Mycology</journal-title></journal-title-group><issn pub-type="ppub">2423-3439</issn><issn pub-type="epub">2423-3420</issn><publisher><publisher-name>Iranian Society of Medical Mycology</publisher-name><publisher-loc>Sari, Iran</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">32420507</article-id><article-id pub-id-type="pmc">7217248</article-id><article-id pub-id-type="doi">10.18502/cmm.6.1.2508</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject></subj-group></article-categories><title-group><article-title>Ultrastructure of hyphal cells of <italic>Trichophyton tonsurans</italic></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Stepanova</surname><given-names>Amaliya</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>de Hoog</surname><given-names>G. Sybren</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Vasilyeva</surname><given-names>Nataliya</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Raznatovskiy</surname><given-names>Konstantin</given-names></name><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Chilina</surname><given-names>Galina</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib></contrib-group><aff id="aff1"><label>1</label>North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia</aff><aff id="aff2"><label>2</label>Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands</aff><aff id="aff3"><label>3</label>Centre of Expertise in Mycology of Radboud University Medical Centre / Canisius Wilhelmina Hospital, Nijmegen, The Netherlands</aff><aff id="aff4"><label>4</label>Department of Dermatovenerology, North-Western State Medical University I.I. Mechnikov, St. Petersburg, Russia</aff><author-notes><corresp id="cor1"><label>*</label>Corresponding author: Amaliya Stepanova, North-Western State Medical University named after I.I. Mechnikov: Kashkin Research Institute of Medical Mycology, Saint Petersburg, Russia. Email: amalya_53@inbox.ru</corresp></author-notes><pub-date pub-type="ppub"><year>2020</year></pub-date><volume>6</volume><issue>1</issue><fpage>42</fpage><lpage>46</lpage><history><date date-type="received"><day>21</day><month>10</month><year>2019</year></date><date date-type="rev-recd"><day>15</day><month>11</month><year>2019</year></date><date date-type="accepted"><day>17</day><month>12</month><year>2019</year></date></history><permissions><license license-type="open-access"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><sec><title>Background and Purpose:</title><p><italic>Trichophyton tonsurans </italic>is a widely distributed anthropophilic dermatophyte causing different diseases of skin. In the literature limited data are available about the morphogenesis of vegetative mycelium of <italic>T. tonsurans</italic> and related anthropophilic dermatophytes. The aim of present study was to describe ultrastructural patterns of development, cellular organellography and septal pore apparatus structure of <italic>in vitro</italic> growing vegetative mycelium of <italic>T. tonsurans</italic>.</p></sec><sec><title>Materials and Methods:</title><p><italic>Trichophyton tonsurans</italic> strain RCPFF 214/898 was grown on solid Czapek’s Agar (CzA) at 28ºС. For investigation of colonies morphology we used methods of light-, scanning and transmission electron microscopy (SEM and TEM).</p></sec><sec><title>Results:</title><p>Differences in morphogenesis of aerial and substrate hyphae were revealed. Mitochondrial reticulum and fibrosinous bodies were shown in <italic>T. tonsurans</italic> for the first time. The septal pore apparatus in hyphal cells of was comprised Woronin bodies and septal pore plugs. Woronin bodies (0.18 µm), located with 1‒4 near the pore, were spherical, membrane-bound, and had a homogeneous, electron-dense content. The cells of aerial and submerged hyphal cells of <italic>T. tonsurans </italic>contain two nuclei.</p></sec><sec><title>Conclusion:</title><p>Mature cells of substrate hyphae appeared more active than comparable cells in the aerial mycelium. During the maturation process, the differences in number and morphology of mitochondria, number of vacuoles, and in the synthesis of different types of storage substances were revealed. Presence of “mitochondrial reticulum” and variable types of storage substances in submerged hyphal cells suggested higher levels of metabolic activity compared to aerial mycelium. </p></sec></abstract><kwd-group><title>Key Words</title><kwd>Dermatophytes</kwd><kwd>Morphogenesis</kwd><kwd>Septal pore apparatus</kwd><kwd>Ultrastructure</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Pays-Bas</li><li>Russie</li></country><region><li>Gueldre</li><li>Utrecht (province)</li></region><settlement><li>Nimègue</li><li>Utrecht</li></settlement></list><tree><country name="Russie"><noRegion><name sortKey="Stepanova, Amaliya" sort="Stepanova, Amaliya" uniqKey="Stepanova A" first="Amaliya" last="Stepanova">Amaliya Stepanova</name></noRegion><name sortKey="Chilina, Galina" sort="Chilina, Galina" uniqKey="Chilina G" first="Galina" last="Chilina">Galina Chilina</name><name sortKey="Raznatovskiy, Konstantin" sort="Raznatovskiy, Konstantin" uniqKey="Raznatovskiy K" first="Konstantin" last="Raznatovskiy">Konstantin Raznatovskiy</name><name sortKey="Vasilyeva, Nataliya" sort="Vasilyeva, Nataliya" uniqKey="Vasilyeva N" first="Nataliya" last="Vasilyeva">Nataliya Vasilyeva</name></country><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="De Hoog, G Sybren" sort="De Hoog, G Sybren" uniqKey="De Hoog G" first="G. Sybren" last="De Hoog">G. Sybren De Hoog</name></region><name sortKey="De Hoog, G Sybren" sort="De Hoog, G Sybren" uniqKey="De Hoog G" first="G. Sybren" last="De Hoog">G. Sybren De Hoog</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000004 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 000004 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= MelampsoraV2
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:7217248
|texte= Ultrastructure of hyphal cells of Trichophyton tonsurans
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:32420507" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MelampsoraV2
| This area was generated with Dilib version V0.6.38. |  |