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Developing a transposon tagging system to isolate rust-resistance genes from flax

Identifieur interne : 000230 ( PascalFrancis/Checkpoint ); précédent : 000229; suivant : 000231

Developing a transposon tagging system to isolate rust-resistance genes from flax

Auteurs : J. G. Ellis [Australie] ; E. J. Finnegan ; G. J. Lawrence

Source :

RBID : Pascal:93-0032775

Descripteurs français

English descriptors

Abstract

A line of flax, homozygous for four genes controlling resistance to flax rust, was transformed with T-DNA vectors carrying the maize transposable elements Ac and Ds to assess whether transposition frequency would be high enough to allow transposon tagging of the resistance genes. Transposition was much less frequent in flax than in Solanaceous hosts such as tobacco, tomato and potato. Transposition frequency in callus tissue, but not in plants, was increased by modifications to the transposase gene of Ac


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Pascal:93-0032775

Le document en format XML

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