Isolation and Diversity Analysis of Resistance Gene Homologues from Switchgrass
Identifieur interne : 000389 ( Ncbi/Merge ); précédent : 000388; suivant : 000390Isolation and Diversity Analysis of Resistance Gene Homologues from Switchgrass
Auteurs : Qihui Zhu [États-Unis] ; Jeffrey L. Bennetzen [États-Unis] ; Shavannor M. Smith [États-Unis]Source :
- G3: Genes|Genomes|Genetics [ 2160-1836 ] ; 2013.
Abstract
Resistance gene homologs (RGHs) were isolated from the switchgrass variety Alamo by a combination of polymerase chain reaction and expressed sequence tag (EST) database mining. Fifty-eight RGHs were isolated by polymerase chain reaction and 295 RGHs were identified in 424,545 switchgrass ESTs. Four nucleotide binding site−leucine-rich repeat RGHs were selected to investigate RGH haplotypic diversity in seven switchgrass varieties chosen for their representation of a broad range of the switchgrass germplasm. Lowland and upland ecotypes were found to be less similar, even from nearby populations, than were more distant populations with similar growth environments. Most (83.5%) of the variability in these four RGHs was found to be attributable to the within-population component. The difference in nucleotide diversity between and within populations was observed to be small, whereas this diversity is maintained to similar degrees at both population and ecotype levels. The results also revealed that the analyzed RGHs were under positive selection in the studied switchgrass accessions. Intragenic recombination was detected in switchgrass RGHs, thereby demonstrating an active genetic process that has the potential to generate new resistance genes with new specificities that might act against newly-arising pathogen races.
Url:
DOI: 10.1534/g3.112.005447
PubMed: 23589518
PubMed Central: 3689800
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Bennetzen</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Genetics, The University of Georgia, Athens, Georgia 30602</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, The University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Smith, Shavannor M" sort="Smith, Shavannor M" uniqKey="Smith S" first="Shavannor M." last="Smith">Shavannor M. Smith</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Plant Pathology, The University of Georgia, Athens, Georgia 30602</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Plant Pathology, The University of Georgia, Athens</wicri:cityArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23589518</idno><idno type="pmc">3689800</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689800</idno><idno type="RBID">PMC:3689800</idno><idno type="doi">10.1534/g3.112.005447</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000867</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000867</idno><idno type="wicri:Area/Pmc/Curation">000867</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000867</idno><idno type="wicri:Area/Pmc/Checkpoint">000876</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000876</idno><idno type="wicri:Area/Ncbi/Merge">000389</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Isolation and Diversity Analysis of Resistance Gene Homologues from Switchgrass</title><author><name sortKey="Zhu, Qihui" sort="Zhu, Qihui" uniqKey="Zhu Q" first="Qihui" last="Zhu">Qihui Zhu</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Genetics, The University of Georgia, Athens, Georgia 30602</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, The University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Bennetzen, Jeffrey L" sort="Bennetzen, Jeffrey L" uniqKey="Bennetzen J" first="Jeffrey L." last="Bennetzen">Jeffrey L. Bennetzen</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Genetics, The University of Georgia, Athens, Georgia 30602</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Genetics, The University of Georgia, Athens</wicri:cityArea></affiliation></author><author><name sortKey="Smith, Shavannor M" sort="Smith, Shavannor M" uniqKey="Smith S" first="Shavannor M." last="Smith">Shavannor M. Smith</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Plant Pathology, The University of Georgia, Athens, Georgia 30602</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Géorgie (États-Unis)</region></placeName><wicri:cityArea>Department of Plant Pathology, The University of Georgia, Athens</wicri:cityArea></affiliation></author></analytic><series><title level="j">G3: Genes|Genomes|Genetics</title><idno type="eISSN">2160-1836</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Resistance gene homologs (RGHs) were isolated from the switchgrass variety Alamo by a combination of polymerase chain reaction and expressed sequence tag (EST) database mining. Fifty-eight RGHs were isolated by polymerase chain reaction and 295 RGHs were identified in 424,545 switchgrass ESTs. Four nucleotide binding site−leucine-rich repeat RGHs were selected to investigate RGH haplotypic diversity in seven switchgrass varieties chosen for their representation of a broad range of the switchgrass germplasm. Lowland and upland ecotypes were found to be less similar, even from nearby populations, than were more distant populations with similar growth environments. Most (83.5%) of the variability in these four RGHs was found to be attributable to the within-population component. The difference in nucleotide diversity between and within populations was observed to be small, whereas this diversity is maintained to similar degrees at both population and ecotype levels. The results also revealed that the analyzed RGHs were under positive selection in the studied switchgrass accessions. Intragenic recombination was detected in switchgrass RGHs, thereby demonstrating an active genetic process that has the potential to generate new resistance genes with new specificities that might act against newly-arising pathogen races.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Altschul, S F" uniqKey="Altschul S">S. F. Altschul</name></author><author><name sortKey="Madden, T L" uniqKey="Madden T">T. L. Madden</name></author><author><name sortKey="Schaffer, A A" uniqKey="Schaffer A">A. A. Schaffer</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J. Zhang</name></author><author><name sortKey="Zhang, Z" uniqKey="Zhang Z">Z. Zhang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ameline Torregrosa, C" uniqKey="Ameline Torregrosa C">C. Ameline-Torregrosa</name></author><author><name sortKey="Wang, B B" uniqKey="Wang B">B. B. Wang</name></author><author><name sortKey="O Leness, M S" uniqKey="O Leness M">M. S. O’bleness</name></author><author><name sortKey="Deshpande, S" uniqKey="Deshpande S">S. Deshpande</name></author><author><name sortKey="Zhu, H" uniqKey="Zhu H">H. Zhu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ashfield, T" uniqKey="Ashfield T">T. Ashfield</name></author><author><name sortKey="Bocian, A" uniqKey="Bocian A">A. Bocian</name></author><author><name sortKey="Held, D" uniqKey="Held D">D. Held</name></author><author><name sortKey="Henk, A D" uniqKey="Henk A">A. D. Henk</name></author><author><name sortKey="Marek, L F" uniqKey="Marek L">L. F. Marek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bakker, E G" uniqKey="Bakker E">E. G. Bakker</name></author><author><name sortKey="Toomajian, C" uniqKey="Toomajian C">C. Toomajian</name></author><author><name sortKey="Kreitman, M" uniqKey="Kreitman M">M. Kreitman</name></author><author><name sortKey="Bergelson, J" uniqKey="Bergelson J">J. Bergelson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Barrett, L G" uniqKey="Barrett L">L. G. Barrett</name></author><author><name sortKey="Thrall, P H" uniqKey="Thrall P">P. H. Thrall</name></author><author><name sortKey="Dobbs, P N" uniqKey="Dobbs P">P. N. Dobbs</name></author><author><name sortKey="Van Der Merwe, M" uniqKey="Van Der Merwe M">M. van der Merwe</name></author><author><name sortKey="Linde, C C" uniqKey="Linde C">C. C. Linde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Baurens, F C" uniqKey="Baurens F">F. C. Baurens</name></author><author><name sortKey="Bocs, S" uniqKey="Bocs S">S. Bocs</name></author><author><name sortKey="Rouard, M" uniqKey="Rouard M">M. Rouard</name></author><author><name sortKey="Matsumoto, T" uniqKey="Matsumoto T">T. Matsumoto</name></author><author><name sortKey="Miller, R" uniqKey="Miller R">R. Miller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bendahmane, A" uniqKey="Bendahmane A">A. Bendahmane</name></author><author><name sortKey="Farnham, G" uniqKey="Farnham G">G. Farnham</name></author><author><name sortKey="Moffett, P" uniqKey="Moffett P">P. Moffett</name></author><author><name sortKey="Baulcombe, D C" uniqKey="Baulcombe D">D. C. Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bennetzen, J L" uniqKey="Bennetzen J">J. L. Bennetzen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bennetzen, J L" uniqKey="Bennetzen J">J. L. Bennetzen</name></author><author><name sortKey="Qin, M M" uniqKey="Qin M">M. M. Qin</name></author><author><name sortKey="Ingels, S" uniqKey="Ingels S">S. Ingels</name></author><author><name sortKey="Ellingboe, A H" uniqKey="Ellingboe A">A. H. Ellingboe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bennetzen, J L" uniqKey="Bennetzen J">J. L. Bennetzen</name></author><author><name sortKey="Schmutz, J" uniqKey="Schmutz J">J. Schmutz</name></author><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name></author><author><name sortKey="Percifield, R" uniqKey="Percifield R">R. Percifield</name></author><author><name sortKey="Hawkins, J" uniqKey="Hawkins J">J. Hawkins</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bent, A F" uniqKey="Bent A">A. F. Bent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bergelson, J" uniqKey="Bergelson J">J. Bergelson</name></author><author><name sortKey="Kreitman, M" uniqKey="Kreitman M">M. Kreitman</name></author><author><name sortKey="Stahl, E A" uniqKey="Stahl E">E. A. Stahl</name></author><author><name sortKey="Tian, D" uniqKey="Tian D">D. Tian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Birney, E" uniqKey="Birney E">E. Birney</name></author><author><name sortKey="Durbin, R" uniqKey="Durbin R">R. Durbin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brunken, J N" uniqKey="Brunken J">J. N. Brunken</name></author><author><name sortKey="Estes, J R" uniqKey="Estes J">J. R. Estes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Caicedo, A L" uniqKey="Caicedo A">A. L. Caicedo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Calenge, F" uniqKey="Calenge F">F. Calenge</name></author><author><name sortKey="Van Der Linden, C G" uniqKey="Van Der Linden C">C. G. Van Der Linden</name></author><author><name sortKey="Van De Weg, E" uniqKey="Van De Weg E">E. Van De Weg</name></author><author><name sortKey="Schouten, H J" uniqKey="Schouten H">H. J. Schouten</name></author><author><name sortKey="Van Arkel, G" uniqKey="Van Arkel G">G. Van Arkel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casler, M" uniqKey="Casler M">M. Casler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casler, M" uniqKey="Casler M">M. Casler</name></author><author><name sortKey="Vogel, K" uniqKey="Vogel K">K. Vogel</name></author><author><name sortKey="Taliaferro, C" uniqKey="Taliaferro C">C. Taliaferro</name></author><author><name sortKey="Wynia, R" uniqKey="Wynia R">R. Wynia</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chin, D B" uniqKey="Chin D">D. B. Chin</name></author><author><name sortKey="Arroyo Garcia, R" uniqKey="Arroyo Garcia R">R. Arroyo-Garcia</name></author><author><name sortKey="Ochoa, O E" uniqKey="Ochoa O">O. E. Ochoa</name></author><author><name sortKey="Kesseli, R V" uniqKey="Kesseli R">R. V. Kesseli</name></author><author><name sortKey="Lavelle, D O" uniqKey="Lavelle D">D. O. Lavelle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Clay, K" uniqKey="Clay K">K. Clay</name></author><author><name sortKey="Kover, P" uniqKey="Kover P">P. Kover</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, N C" uniqKey="Collins N">N. C. Collins</name></author><author><name sortKey="Webb, C A" uniqKey="Webb C">C. A. Webb</name></author><author><name sortKey="Seah, S" uniqKey="Seah S">S. Seah</name></author><author><name sortKey="Ellis, J G" uniqKey="Ellis J">J. G. Ellis</name></author><author><name sortKey="Hulbert, S H" uniqKey="Hulbert S">S. H. Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cortese, L M" uniqKey="Cortese L">L. M. Cortese</name></author><author><name sortKey="Honig, J" uniqKey="Honig J">J. Honig</name></author><author><name sortKey="Miller, C" uniqKey="Miller C">C. Miller</name></author><author><name sortKey="Bonos, S A" uniqKey="Bonos S">S. A. Bonos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dangl, J L" uniqKey="Dangl J">J. L. Dangl</name></author><author><name sortKey="Jones, J D G" uniqKey="Jones J">J. D. G. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diaz, V" uniqKey="Diaz V">V. Diaz</name></author><author><name sortKey="Ferrer, E" uniqKey="Ferrer E">E. Ferrer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dilbirligi, M" uniqKey="Dilbirligi M">M. Dilbirligi</name></author><author><name sortKey="Gill, K S" uniqKey="Gill K">K. S. Gill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ding, J" uniqKey="Ding J">J. Ding</name></author><author><name sortKey="Zhang, W" uniqKey="Zhang W">W. Zhang</name></author><author><name sortKey="Jing, Z" uniqKey="Jing Z">Z. Jing</name></author><author><name sortKey="Chen, J Q" uniqKey="Chen J">J. Q. Chen</name></author><author><name sortKey="Tian, D" uniqKey="Tian D">D. Tian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dixon, M S" uniqKey="Dixon M">M. S. Dixon</name></author><author><name sortKey="Hatzixanthis, K" uniqKey="Hatzixanthis K">K. Hatzixanthis</name></author><author><name sortKey="Jones, D A" uniqKey="Jones D">D. A. Jones</name></author><author><name sortKey="Harrison, K" uniqKey="Harrison K">K. Harrison</name></author><author><name sortKey="Jones, J D G" uniqKey="Jones J">J. D. G. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Donald, T M" uniqKey="Donald T">T. M. Donald</name></author><author><name sortKey="Pellerone, F" uniqKey="Pellerone F">F. Pellerone</name></author><author><name sortKey="Adam Blondon, A F" uniqKey="Adam Blondon A">A. F. Adam-Blondon</name></author><author><name sortKey="Bouquet, A" uniqKey="Bouquet A">A. Bouquet</name></author><author><name sortKey="Thomas, M R" uniqKey="Thomas M">M. R. Thomas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ellis, J G" uniqKey="Ellis J">J. G. Ellis</name></author><author><name sortKey="Lawrence, G J" uniqKey="Lawrence G">G. J. Lawrence</name></author><author><name sortKey="Luck, J E" uniqKey="Luck J">J. E. Luck</name></author><author><name sortKey="Dodds, P N" uniqKey="Dodds P">P. N. Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Glynn, N C" uniqKey="Glynn N">N. C. Glynn</name></author><author><name sortKey="Comstock, J C" uniqKey="Comstock J">J. C. Comstock</name></author><author><name sortKey="Sood, S G" uniqKey="Sood S">S. G. Sood</name></author><author><name sortKey="Dang, P M" uniqKey="Dang P">P. M. Dang</name></author><author><name sortKey="Chaparro, J X" uniqKey="Chaparro J">J. X. Chaparro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Grant, M R" uniqKey="Grant M">M. R. Grant</name></author><author><name sortKey="Godiard, L" uniqKey="Godiard L">L. Godiard</name></author><author><name sortKey="Straube, E" uniqKey="Straube E">E. Straube</name></author><author><name sortKey="Ashfield, T" uniqKey="Ashfield T">T. Ashfield</name></author><author><name sortKey="Lewald, J" uniqKey="Lewald J">J. Lewald</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gunter, L E" uniqKey="Gunter L">L. E. Gunter</name></author><author><name sortKey="Tuskan, G A" uniqKey="Tuskan G">G. A. Tuskan</name></author><author><name sortKey="Wullschleger, S D" uniqKey="Wullschleger S">S. D. Wullschleger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hall, T A" uniqKey="Hall T">T. A. Hall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hammond Kosack, K E" uniqKey="Hammond Kosack K">K. E. Hammond-Kosack</name></author><author><name sortKey="Jones, J D G" uniqKey="Jones J">J. D. G. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hao, D C" uniqKey="Hao D">D. C. Hao</name></author><author><name sortKey="Huang, B L" uniqKey="Huang B">B. L. Huang</name></author><author><name sortKey="Chen, S L" uniqKey="Chen S">S. L. Chen</name></author><author><name sortKey="Mu, J" uniqKey="Mu J">J. Mu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huang, S" uniqKey="Huang S">S. Huang</name></author><author><name sortKey="Su, X" uniqKey="Su X">X. Su</name></author><author><name sortKey="Haselkorn, R" uniqKey="Haselkorn R">R. Haselkorn</name></author><author><name sortKey="Gornicki, P" uniqKey="Gornicki P">P. Gornicki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hudson, R R" uniqKey="Hudson R">R. R. Hudson</name></author><author><name sortKey="Kreitman, M" uniqKey="Kreitman M">M. Kreitman</name></author><author><name sortKey="Aguade, M" uniqKey="Aguade M">M. Aguade</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulbert, S H" uniqKey="Hulbert S">S. H. Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hulbert, S H" uniqKey="Hulbert S">S. H. Hulbert</name></author><author><name sortKey="Webb, C A" uniqKey="Webb C">C. A. Webb</name></author><author><name sortKey="Smith, S M" uniqKey="Smith S">S. M. Smith</name></author><author><name sortKey="Sun, Q" uniqKey="Sun Q">Q. Sun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hultquist, S J" uniqKey="Hultquist S">S. J. Hultquist</name></author><author><name sortKey="Vogel, K" uniqKey="Vogel K">K. Vogel</name></author><author><name sortKey="Lee, D" uniqKey="Lee D">D. Lee</name></author><author><name sortKey="Arumuganathan, K" uniqKey="Arumuganathan K">K. Arumuganathan</name></author><author><name sortKey="Kaeppler, S" uniqKey="Kaeppler S">S. Kaeppler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hultquist, S J" uniqKey="Hultquist S">S. J. Hultquist</name></author><author><name sortKey="Vogel, K" uniqKey="Vogel K">K. Vogel</name></author><author><name sortKey="Lee, D" uniqKey="Lee D">D. Lee</name></author><author><name sortKey="Arumuganathan, K" uniqKey="Arumuganathan K">K. Arumuganathan</name></author><author><name sortKey="Kaeppler, S" uniqKey="Kaeppler S">S. Kaeppler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hwang, C F" uniqKey="Hwang C">C. F. Hwang</name></author><author><name sortKey="Williamson, V M" uniqKey="Williamson V">V. M. Williamson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jiang, H" uniqKey="Jiang H">H. Jiang</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C. Wang</name></author><author><name sortKey="Ping, L" uniqKey="Ping L">L. Ping</name></author><author><name sortKey="Tian, D" uniqKey="Tian D">D. Tian</name></author><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kanazin, V" uniqKey="Kanazin V">V. Kanazin</name></author><author><name sortKey="Marek, L F" uniqKey="Marek L">L. F. Marek</name></author><author><name sortKey="Shoemaker, R C" uniqKey="Shoemaker R">R. C. Shoemaker</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kobe, B" uniqKey="Kobe B">B. Kobe</name></author><author><name sortKey="Deisenhofer, J" uniqKey="Deisenhofer J">J. Deisenhofer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kohler, A" uniqKey="Kohler A">A. Kohler</name></author><author><name sortKey="Rinaldi, C" uniqKey="Rinaldi C">C. Rinaldi</name></author><author><name sortKey="Duplessis, S" uniqKey="Duplessis S">S. Duplessis</name></author><author><name sortKey="Baucher, M" uniqKey="Baucher M">M. Baucher</name></author><author><name sortKey="Geelen, D" uniqKey="Geelen D">D. Geelen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Larkin, M A" uniqKey="Larkin M">M. A. Larkin</name></author><author><name sortKey="Blackshields, G" uniqKey="Blackshields G">G. Blackshields</name></author><author><name sortKey="Brown, N P" uniqKey="Brown N">N. P. Brown</name></author><author><name sortKey="Chenna, R" uniqKey="Chenna R">R. Chenna</name></author><author><name sortKey="Mcgettigan, P A" uniqKey="Mcgettigan P">P. A. Mcgettigan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, B" uniqKey="Lee B">B. Lee</name></author><author><name sortKey="Hong, T" uniqKey="Hong T">T. Hong</name></author><author><name sortKey="Byun, S J" uniqKey="Byun S">S. J. Byun</name></author><author><name sortKey="Woo, T" uniqKey="Woo T">T. Woo</name></author><author><name sortKey="Choi, Y J" uniqKey="Choi Y">Y. J. Choi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leister, R T" uniqKey="Leister R">R. T. Leister</name></author><author><name sortKey="Katagiri, F" uniqKey="Katagiri F">F. Katagiri</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Librado, P" uniqKey="Librado P">P. Librado</name></author><author><name sortKey="Rozas, J" uniqKey="Rozas J">J. Rozas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lisch, D" uniqKey="Lisch D">D. Lisch</name></author><author><name sortKey="Bennetzen, J L" uniqKey="Bennetzen J">J. L. Bennetzen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Luck, J E" uniqKey="Luck J">J. E. Luck</name></author><author><name sortKey="Lawrence, G J" uniqKey="Lawrence G">G. J. Lawrence</name></author><author><name sortKey="Dodds, P N" uniqKey="Dodds P">P. N. Dodds</name></author><author><name sortKey="Shepherd, K W" uniqKey="Shepherd K">K. W. Shepherd</name></author><author><name sortKey="Ellis, J G" uniqKey="Ellis J">J. G. Ellis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lukashin, A V" uniqKey="Lukashin A">A. V. Lukashin</name></author><author><name sortKey="Borodovsky, M" uniqKey="Borodovsky M">M. Borodovsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mclaughlin, S" uniqKey="Mclaughlin S">S. McLaughlin</name></author><author><name sortKey="Bouton, J" uniqKey="Bouton J">J. Bouton</name></author><author><name sortKey="Bransby, D" uniqKey="Bransby D">D. Bransby</name></author><author><name sortKey="Conger, B" uniqKey="Conger B">B. Conger</name></author><author><name sortKey="Ocumpaugh, W" uniqKey="Ocumpaugh W">W. Ocumpaugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meyers, B C" uniqKey="Meyers B">B. C. Meyers</name></author><author><name sortKey="Kozik, A" uniqKey="Kozik A">A. Kozik</name></author><author><name sortKey="Griego, A" uniqKey="Griego A">A. Griego</name></author><author><name sortKey="Kuang, H" uniqKey="Kuang H">H. Kuang</name></author><author><name sortKey="Michelmore, R W" uniqKey="Michelmore R">R. W. Michelmore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Michelmore, R W" uniqKey="Michelmore R">R. W. Michelmore</name></author><author><name sortKey="Meyers, B C" uniqKey="Meyers B">B. C. Meyers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Missaoui, A M" uniqKey="Missaoui A">A. M. Missaoui</name></author><author><name sortKey="Paterson, A H" uniqKey="Paterson A">A. H. Paterson</name></author><author><name sortKey="Bouton, J H" uniqKey="Bouton J">J. H. Bouton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Moffett, P" uniqKey="Moffett P">P. Moffett</name></author><author><name sortKey="Farnham, G" uniqKey="Farnham G">G. Farnham</name></author><author><name sortKey="Peart, J" uniqKey="Peart J">J. Peart</name></author><author><name sortKey="Baulcombe, D C" uniqKey="Baulcombe D">D. C. Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mondrag N Palomino, M" uniqKey="Mondrag N Palomino M">M. Mondragón-Palomino</name></author><author><name sortKey="Meyers, B C" uniqKey="Meyers B">B. C. Meyers</name></author><author><name sortKey="Michelmore, R W" uniqKey="Michelmore R">R. W. Michelmore</name></author><author><name sortKey="Gaut, B S" uniqKey="Gaut B">B. S. Gaut</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Monosi, B" uniqKey="Monosi B">B. Monosi</name></author><author><name sortKey="Wisser, R J" uniqKey="Wisser R">R. J. Wisser</name></author><author><name sortKey="Pennill, L" uniqKey="Pennill L">L. Pennill</name></author><author><name sortKey="Hulbert, S H" uniqKey="Hulbert S">S. H. Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Murray, M G" uniqKey="Murray M">M. G. Murray</name></author><author><name sortKey="Thompson, W F" uniqKey="Thompson W">W. F. Thompson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nagy, E D" uniqKey="Nagy E">E. D. Nagy</name></author><author><name sortKey="Bennetzen, J L" uniqKey="Bennetzen J">J. L. Bennetzen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Narasimhamoorthy, B" uniqKey="Narasimhamoorthy B">B. Narasimhamoorthy</name></author><author><name sortKey="Saha, M C" uniqKey="Saha M">M. C. Saha</name></author><author><name sortKey="Swaller, T" uniqKey="Swaller T">T. Swaller</name></author><author><name sortKey="Bouton, J H" uniqKey="Bouton J">J. H. Bouton</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nei, M" uniqKey="Nei M">M. Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nybom, H" uniqKey="Nybom H">H. Nybom</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, Q" uniqKey="Pan Q">Q. Pan</name></author><author><name sortKey="Wendel, J" uniqKey="Wendel J">J. Wendel</name></author><author><name sortKey="Fluhr, R" uniqKey="Fluhr R">R. Fluhr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Parniske, M" uniqKey="Parniske M">M. Parniske</name></author><author><name sortKey="Hammond Kosack, K E" uniqKey="Hammond Kosack K">K. E. Hammond-Kosack</name></author><author><name sortKey="Golstein, C" uniqKey="Golstein C">C. Golstein</name></author><author><name sortKey="Thomas, C M" uniqKey="Thomas C">C. M. Thomas</name></author><author><name sortKey="Jones, D A" uniqKey="Jones D">D. A. Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peakall, R O D" uniqKey="Peakall R">R. O. D. Peakall</name></author><author><name sortKey="Smouse, P E" uniqKey="Smouse P">P. E. Smouse</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Radwan, O" uniqKey="Radwan O">O. Radwan</name></author><author><name sortKey="Gandhi, S" uniqKey="Gandhi S">S. Gandhi</name></author><author><name sortKey="Heesacker, A" uniqKey="Heesacker A">A. Heesacker</name></author><author><name sortKey="Whitaker, B" uniqKey="Whitaker B">B. Whitaker</name></author><author><name sortKey="Taylor, C" uniqKey="Taylor C">C. Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ramakrishna, W" uniqKey="Ramakrishna W">W. Ramakrishna</name></author><author><name sortKey="Emberton, J" uniqKey="Emberton J">J. Emberton</name></author><author><name sortKey="Sanmiguel, P" uniqKey="Sanmiguel P">P. Sanmiguel</name></author><author><name sortKey="Ogden, M" uniqKey="Ogden M">M. Ogden</name></author><author><name sortKey="Llaca, V" uniqKey="Llaca V">V. Llaca</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ravensdale, M" uniqKey="Ravensdale M">M. Ravensdale</name></author><author><name sortKey="Nemri, A" uniqKey="Nemri A">A. Nemri</name></author><author><name sortKey="Thrall, P H" uniqKey="Thrall P">P. H. Thrall</name></author><author><name sortKey="Ellis, J G" uniqKey="Ellis J">J. G. Ellis</name></author><author><name sortKey="Dodds, P N" uniqKey="Dodds P">P. N. Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ravensdale, M" uniqKey="Ravensdale M">M. Ravensdale</name></author><author><name sortKey="Bernoux, M" uniqKey="Bernoux M">M. Bernoux</name></author><author><name sortKey="Ve, T" uniqKey="Ve T">T. Ve</name></author><author><name sortKey="Kobe, B" uniqKey="Kobe B">B. Kobe</name></author><author><name sortKey="Thrall, P H" uniqKey="Thrall P">P. H. Thrall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rice, P" uniqKey="Rice P">P. Rice</name></author><author><name sortKey="Longden, I" uniqKey="Longden I">I. Longden</name></author><author><name sortKey="Bleasby, A" uniqKey="Bleasby A">A. Bleasby</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rose, L E" uniqKey="Rose L">L. E. Rose</name></author><author><name sortKey="Michelmore, R W" uniqKey="Michelmore R">R. W. Michelmore</name></author><author><name sortKey="Langley, C H" uniqKey="Langley C">C. H. Langley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rouse, M N" uniqKey="Rouse M">M. N. Rouse</name></author><author><name sortKey="Saleh, A A" uniqKey="Saleh A">A. A. Saleh</name></author><author><name sortKey="Seck, A" uniqKey="Seck A">A. Seck</name></author><author><name sortKey="Keeler, K H" uniqKey="Keeler K">K. H. Keeler</name></author><author><name sortKey="Travers, S E" uniqKey="Travers S">S. E. Travers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saitou, N" uniqKey="Saitou N">N. Saitou</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M. Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanderson, M A" uniqKey="Sanderson M">M. A. Sanderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sela, H" uniqKey="Sela H">H. Sela</name></author><author><name sortKey="Cheng, J" uniqKey="Cheng J">J. Cheng</name></author><author><name sortKey="Jun, Y" uniqKey="Jun Y">Y. Jun</name></author><author><name sortKey="Nevo, E" uniqKey="Nevo E">E. Nevo</name></author><author><name sortKey="Fahima, T" uniqKey="Fahima T">T. Fahima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, S M" uniqKey="Smith S">S. M. Smith</name></author><author><name sortKey="Hulbert, S H" uniqKey="Hulbert S">S. H. Hulbert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, W Y" uniqKey="Song W">W. Y. Song</name></author><author><name sortKey="Pi, L Y" uniqKey="Pi L">L. Y. Pi</name></author><author><name sortKey="Wang, G L" uniqKey="Wang G">G. L. Wang</name></author><author><name sortKey="Gardner, J" uniqKey="Gardner J">J. Gardner</name></author><author><name sortKey="Holsten, T" uniqKey="Holsten T">T. Holsten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tajima, F" uniqKey="Tajima F">F. Tajima</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tameling, W I L" uniqKey="Tameling W">W. I. L. Tameling</name></author><author><name sortKey="Elzinga, S D J" uniqKey="Elzinga S">S. D. J. Elzinga</name></author><author><name sortKey="Darmin, P S" uniqKey="Darmin P">P. S. Darmin</name></author><author><name sortKey="Vossen, J H" uniqKey="Vossen J">J. H. Vossen</name></author><author><name sortKey="Takken, F L W" uniqKey="Takken F">F. L. W. Takken</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tamura, K" uniqKey="Tamura K">K. Tamura</name></author><author><name sortKey="Peterson, D" uniqKey="Peterson D">D. Peterson</name></author><author><name sortKey="Peterson, N" uniqKey="Peterson N">N. Peterson</name></author><author><name sortKey="Stecher, G" uniqKey="Stecher G">G. Stecher</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M. Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tan, X" uniqKey="Tan X">X. Tan</name></author><author><name sortKey="Meyers, B C" uniqKey="Meyers B">B. C. Meyers</name></author><author><name sortKey="Kozik, A" uniqKey="Kozik A">A. Kozik</name></author><author><name sortKey="West, M L" uniqKey="West M">M. L. West</name></author><author><name sortKey="Morgante, M" uniqKey="Morgante M">M. Morgante</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Z X" uniqKey="Wang Z">Z. X. Wang</name></author><author><name sortKey="Yano, M" uniqKey="Yano M">M. Yano</name></author><author><name sortKey="Yamanouchi, U" uniqKey="Yamanouchi U">U. Yamanouchi</name></author><author><name sortKey="Iwamoto, M" uniqKey="Iwamoto M">M. Iwamoto</name></author><author><name sortKey="Monna, L" uniqKey="Monna L">L. Monna</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watterson, G A" uniqKey="Watterson G">G. A. Watterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wei, F" uniqKey="Wei F">F. Wei</name></author><author><name sortKey="Gobelman Werner, K" uniqKey="Gobelman Werner K">K. Gobelman-Werner</name></author><author><name sortKey="Morroll, S M" uniqKey="Morroll S">S. M. Morroll</name></author><author><name sortKey="Kurth, J" uniqKey="Kurth J">J. Kurth</name></author><author><name sortKey="Mao, L" uniqKey="Mao L">L. Mao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Welter, L J" uniqKey="Welter L">L. J. Welter</name></author><author><name sortKey="Gokturk Bayder, N" uniqKey="Gokturk Bayder N">N. Göktürk-Bayder</name></author><author><name sortKey="Akkurt, M" uniqKey="Akkurt M">M. Akkurt</name></author><author><name sortKey="Maul, E" uniqKey="Maul E">E. Maul</name></author><author><name sortKey="Eibach, R" uniqKey="Eibach R">R. Eibach</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wessler, S R" uniqKey="Wessler S">S. R. Wessler</name></author><author><name sortKey="Bureau, T E" uniqKey="Bureau T">T. E. Bureau</name></author><author><name sortKey="White, S E" uniqKey="White S">S. E. White</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author><author><name sortKey="Gu, T" uniqKey="Gu T">T. Gu</name></author><author><name sortKey="Pan, C" uniqKey="Pan C">C. Pan</name></author><author><name sortKey="Feng, Z" uniqKey="Feng Z">Z. Feng</name></author><author><name sortKey="Ding, J" uniqKey="Ding J">J. Ding</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, S" uniqKey="Yang S">S. Yang</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X. Zhang</name></author><author><name sortKey="Yue, J X" uniqKey="Yue J">J. X. Yue</name></author><author><name sortKey="Tian, D" uniqKey="Tian D">D. Tian</name></author><author><name sortKey="Chen, J Q" uniqKey="Chen J">J. Q. Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, Z" uniqKey="Yang Z">Z. Yang</name></author><author><name sortKey="Nielsen, R" uniqKey="Nielsen R">R. Nielsen</name></author><author><name sortKey="Goldman, N" uniqKey="Goldman N">N. Goldman</name></author><author><name sortKey="Pedersen, A M K" uniqKey="Pedersen A">A. M. K. Pedersen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yao, H" uniqKey="Yao H">H. Yao</name></author><author><name sortKey="Guo, L" uniqKey="Guo L">L. Guo</name></author><author><name sortKey="Fu, Y" uniqKey="Fu Y">Y. Fu</name></author><author><name sortKey="Borsuk, L A" uniqKey="Borsuk L">L. A. Borsuk</name></author><author><name sortKey="Wen, T J" uniqKey="Wen T">T. J. Wen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Young, N D" uniqKey="Young N">N. D. Young</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, T" uniqKey="Zhou T">T. Zhou</name></author><author><name sortKey="Wang, Y" uniqKey="Wang Y">Y. Wang</name></author><author><name sortKey="Chen, J Q" uniqKey="Chen J">J. Q. Chen</name></author><author><name sortKey="Araki, H" uniqKey="Araki H">H. Araki</name></author><author><name sortKey="Jing, Z" uniqKey="Jing Z">Z. Jing</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">G3 (Bethesda)</journal-id><journal-id journal-id-type="iso-abbrev">Genetics</journal-id><journal-id journal-id-type="hwp">G3: Genes, Genomes, Genetics</journal-id><journal-id journal-id-type="pmc">G3: Genes, Genomes, Genetics</journal-id><journal-id journal-id-type="publisher-id">G3: Genes, Genomes, Genetics</journal-id><journal-title-group><journal-title>G3: Genes|Genomes|Genetics</journal-title></journal-title-group><issn pub-type="epub">2160-1836</issn><publisher><publisher-name>Genetics Society of America</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23589518</article-id><article-id pub-id-type="pmc">3689800</article-id><article-id pub-id-type="publisher-id">GGG_005447</article-id><article-id pub-id-type="doi">10.1534/g3.112.005447</article-id><article-categories><subj-group subj-group-type="heading"><subject>Investigations</subject></subj-group></article-categories><title-group><article-title>Isolation and Diversity Analysis of Resistance Gene Homologues from Switchgrass</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhu</surname><given-names>Qihui</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Bennetzen</surname><given-names>Jeffrey L.</given-names></name><xref ref-type="aff" rid="aff1">*</xref></contrib><contrib contrib-type="author"><name><surname>Smith</surname><given-names>Shavannor M.</given-names></name><xref ref-type="aff" rid="aff2"><sup>†</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1"><label>*</label>Department of Genetics, The University of Georgia, Athens, Georgia 30602</aff><aff id="aff2"><label>†</label>Department of Plant Pathology, The University of Georgia, Athens, Georgia 30602</aff></contrib-group><author-notes><fn id="afn2"><p>Supporting information is available online at <ext-link ext-link-type="uri" xlink:href="http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.112.005447/-/DC1">http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.112.005447/-/DC1</ext-link></p></fn><fn id="afn3"><p>Sequence data from this article have been deposited in Genbank under accession nos. JN231541-JN232038</p></fn><corresp id="cor1"><label>1</label>Corresponding author: Department of Plant Pathology, Miller Plant Sciences 4309, University of Georgia, Athens, GA 30602. E-mail: <email>shavs@uga.edu</email></corresp></author-notes><pub-date pub-type="epub"><day>1</day><month>6</month><year>2013</year></pub-date><pub-date pub-type="collection"><month>6</month><year>2013</year></pub-date><volume>3</volume><issue>6</issue><fpage>1031</fpage><lpage>1042</lpage><history><date date-type="received"><day>18</day><month>12</month><year>2012</year></date><date date-type="accepted"><day>10</day><month>4</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013 Zhu <italic>et al.</italic></copyright-statement><copyright-year>2013</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by/3.0/"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/3.0/">http://creativecommons.org/licenses/by/3.0/</ext-link>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="1031.pdf"></self-uri><abstract><p>Resistance gene homologs (RGHs) were isolated from the switchgrass variety Alamo by a combination of polymerase chain reaction and expressed sequence tag (EST) database mining. Fifty-eight RGHs were isolated by polymerase chain reaction and 295 RGHs were identified in 424,545 switchgrass ESTs. Four nucleotide binding site−leucine-rich repeat RGHs were selected to investigate RGH haplotypic diversity in seven switchgrass varieties chosen for their representation of a broad range of the switchgrass germplasm. Lowland and upland ecotypes were found to be less similar, even from nearby populations, than were more distant populations with similar growth environments. Most (83.5%) of the variability in these four RGHs was found to be attributable to the within-population component. The difference in nucleotide diversity between and within populations was observed to be small, whereas this diversity is maintained to similar degrees at both population and ecotype levels. The results also revealed that the analyzed RGHs were under positive selection in the studied switchgrass accessions. Intragenic recombination was detected in switchgrass RGHs, thereby demonstrating an active genetic process that has the potential to generate new resistance genes with new specificities that might act against newly-arising pathogen races.</p></abstract><kwd-group><kwd>resistance gene homologue (RGH)</kwd><kwd>genetic diversity</kwd><kwd>population structure</kwd><kwd>switchgrass</kwd><kwd>bioenergy crop</kwd><kwd>NBS-LRR</kwd></kwd-group><counts><page-count count="12"></page-count></counts><custom-meta-group><custom-meta><meta-name> DJS Export </meta-name><meta-value>v1</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Géorgie (États-Unis)</li></region></list><tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Zhu, Qihui" sort="Zhu, Qihui" uniqKey="Zhu Q" first="Qihui" last="Zhu">Qihui Zhu</name></region><name sortKey="Bennetzen, Jeffrey L" sort="Bennetzen, Jeffrey L" uniqKey="Bennetzen J" first="Jeffrey L." last="Bennetzen">Jeffrey L. Bennetzen</name><name sortKey="Smith, Shavannor M" sort="Smith, Shavannor M" uniqKey="Smith S" first="Shavannor M." last="Smith">Shavannor M. Smith</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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