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The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina

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The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina

Auteurs : Xiben Wang ; Mingzhe Z. Che ; Hala B. Khalil ; Brent D. Mccallum ; Guus Bakkeren ; Christof Rampitsch ; Barry J. Saville

Source :

Environmental Microbiology [ 1462-2912 ] ; 2020.

RBID : PMC:7496513

Abstract

Summary

Reactive oxygen species (ROS) play an important role during host–pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that Puccinia triticina (Pt) generates ROS, including superoxide, H₂O₂ and hydroxyl radicals, during wheat infection. Through pharmacological inhibition, we found that ROS are critical for both Pt urediniospore germination and pathogenic development on wheat. A comparative RNA‐Seq analysis of different stages of Pt infection process revealed 291 putative Pt genes associated with the oxidation–reduction process. Thirty‐seven of these genes encode known proteins. The expressions of five Pt genes, including PtNoxA, PtNoxB, PtNoxR, PtCat and PtSod, were subsequently verified using RT‐qPCR analysis. The results show that the expressions of PtNoxA, PtNoxB, PtNoxR, PtCat and PtSod are up‐regulated during urediniospore germination. In comparison, the expressions of PtNoxA, PtNoxB, PtNoxR and PtCat are down‐regulated during wheat infection from 12 to 120 h after inoculation (HAI), whereas the expression of PtSod is up‐regulated with a peak of expression at 120 HAI. We conclude that ROS are critical for the full virulence of Pt and a coordinate down‐regulation of PtNox genes may be important for successful infection in wheat.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496513

DOI: 10.1111/1462-2920.15063
PubMed: 32390310
PubMed Central: 7496513

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<p>Reactive oxygen species (ROS) play an important role during host–pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that <italic>Puccinia triticina</italic>
 (<italic>Pt</italic>
) generates ROS, including superoxide, H<sub>2</sub>
O<sub>2</sub>
 and hydroxyl radicals, during wheat infection. Through pharmacological inhibition, we found that ROS are critical for both <italic>Pt</italic>
 urediniospore germination and pathogenic development on wheat. A comparative RNA‐Seq analysis of different stages of <italic>Pt</italic>
 infection process revealed 291 putative <italic>Pt</italic>
 genes associated with the oxidation–reduction process. Thirty‐seven of these genes encode known proteins. The expressions of five <italic>Pt</italic>
 genes, including <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
, <italic>PtCat</italic>
 and <italic>PtSod</italic>
, were subsequently verified using RT‐qPCR analysis. The results show that the expressions of <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
, <italic>PtCat</italic>
 and <italic>PtSod</italic>
 are up‐regulated during urediniospore germination. In comparison, the expressions of <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
 and <italic>PtCat</italic>
 are down‐regulated during wheat infection from 12 to 120 h after inoculation (HAI), whereas the expression of <italic>PtSod</italic>
 is up‐regulated with a peak of expression at 120 HAI. We conclude that ROS are critical for the full virulence of <italic>Pt</italic>
 and a coordinate down‐regulation of <italic>PtNox</italic>
 genes may be important for successful infection in wheat.</p>
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The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina

The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina

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Abstract

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