The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina
Identifieur interne : 000B51 ( Ncbi/Curation ); précédent : 000B50; suivant : 000B52The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina
Auteurs : Xiben Wang ; Mingzhe Z. Che ; Hala B. Khalil ; Brent D. Mccallum ; Guus Bakkeren ; Christof Rampitsch ; Barry J. SavilleSource :
- Environmental Microbiology [ 1462-2912 ] ; 2020.
Abstract
Reactive oxygen species (ROS) play an important role during host–pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that
Url:
DOI: 10.1111/1462-2920.15063
PubMed: 32390310
PubMed Central: 7496513
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000762
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000762
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000010
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000B51
Links to Exploration step
PMC:7496513Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The role of reactive oxygen species in the virulence of wheat leaf rust fungus <italic>Puccinia triticina</italic>
</title>
<author><name sortKey="Wang, Xiben" sort="Wang, Xiben" uniqKey="Wang X" first="Xiben" last="Wang">Xiben Wang</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Che, Mingzhe Z" sort="Che, Mingzhe Z" uniqKey="Che M" first="Mingzhe Z." last="Che">Mingzhe Z. Che</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="emi15063-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Khalil, Hala B" sort="Khalil, Hala B" uniqKey="Khalil H" first="Hala B." last="Khalil">Hala B. Khalil</name>
<affiliation><nlm:aff id="emi15063-aff-0003"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="emi15063-aff-0004"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mccallum, Brent D" sort="Mccallum, Brent D" uniqKey="Mccallum B" first="Brent D." last="Mccallum">Brent D. Mccallum</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name>
<affiliation><nlm:aff id="emi15063-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rampitsch, Christof" sort="Rampitsch, Christof" uniqKey="Rampitsch C" first="Christof" last="Rampitsch">Christof Rampitsch</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Saville, Barry J" sort="Saville, Barry J" uniqKey="Saville B" first="Barry J." last="Saville">Barry J. Saville</name>
<affiliation><nlm:aff id="emi15063-aff-0005"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">32390310</idno>
<idno type="pmc">7496513</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7496513</idno>
<idno type="RBID">PMC:7496513</idno>
<idno type="doi">10.1111/1462-2920.15063</idno>
<date when="2020">2020</date>
<idno type="wicri:Area/Pmc/Corpus">000762</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000762</idno>
<idno type="wicri:Area/Pmc/Curation">000762</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000762</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000010</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000010</idno>
<idno type="wicri:Area/Ncbi/Merge">000B51</idno>
<idno type="wicri:Area/Ncbi/Curation">000B51</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">The role of reactive oxygen species in the virulence of wheat leaf rust fungus <italic>Puccinia triticina</italic>
</title>
<author><name sortKey="Wang, Xiben" sort="Wang, Xiben" uniqKey="Wang X" first="Xiben" last="Wang">Xiben Wang</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Che, Mingzhe Z" sort="Che, Mingzhe Z" uniqKey="Che M" first="Mingzhe Z." last="Che">Mingzhe Z. Che</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="emi15063-aff-0002"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Khalil, Hala B" sort="Khalil, Hala B" uniqKey="Khalil H" first="Hala B." last="Khalil">Hala B. Khalil</name>
<affiliation><nlm:aff id="emi15063-aff-0003"></nlm:aff>
</affiliation>
<affiliation><nlm:aff id="emi15063-aff-0004"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Mccallum, Brent D" sort="Mccallum, Brent D" uniqKey="Mccallum B" first="Brent D." last="Mccallum">Brent D. Mccallum</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bakkeren, Guus" sort="Bakkeren, Guus" uniqKey="Bakkeren G" first="Guus" last="Bakkeren">Guus Bakkeren</name>
<affiliation><nlm:aff id="emi15063-aff-0003"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Rampitsch, Christof" sort="Rampitsch, Christof" uniqKey="Rampitsch C" first="Christof" last="Rampitsch">Christof Rampitsch</name>
<affiliation><nlm:aff id="emi15063-aff-0001"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Saville, Barry J" sort="Saville, Barry J" uniqKey="Saville B" first="Barry J." last="Saville">Barry J. Saville</name>
<affiliation><nlm:aff id="emi15063-aff-0005"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Environmental Microbiology</title>
<idno type="ISSN">1462-2912</idno>
<idno type="eISSN">1462-2920</idno>
<imprint><date when="2020">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Summary</title>
<p>Reactive oxygen species (ROS) play an important role during host–pathogen interactions and are often an indication of induced host defence responses. In this study, we demonstrate for the first time that <italic>Puccinia triticina</italic>
(<italic>Pt</italic>
) generates ROS, including superoxide, H<sub>2</sub>
O<sub>2</sub>
and hydroxyl radicals, during wheat infection. Through pharmacological inhibition, we found that ROS are critical for both <italic>Pt</italic>
urediniospore germination and pathogenic development on wheat. A comparative RNA‐Seq analysis of different stages of <italic>Pt</italic>
infection process revealed 291 putative <italic>Pt</italic>
genes associated with the oxidation–reduction process. Thirty‐seven of these genes encode known proteins. The expressions of five <italic>Pt</italic>
genes, including <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
, <italic>PtCat</italic>
and <italic>PtSod</italic>
, were subsequently verified using RT‐qPCR analysis. The results show that the expressions of <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
, <italic>PtCat</italic>
and <italic>PtSod</italic>
are up‐regulated during urediniospore germination. In comparison, the expressions of <italic>PtNoxA</italic>
, <italic>PtNoxB</italic>
, <italic>PtNoxR</italic>
and <italic>PtCat</italic>
are down‐regulated during wheat infection from 12 to 120 h after inoculation (HAI), whereas the expression of <italic>PtSod</italic>
is up‐regulated with a peak of expression at 120 HAI. We conclude that ROS are critical for the full virulence of <italic>Pt</italic>
and a coordinate down‐regulation of <italic>PtNox</italic>
genes may be important for successful infection in wheat.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/MelampsoraV2/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B51 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000B51 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Bois |area= MelampsoraV2 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:7496513 |texte= The role of reactive oxygen species in the virulence of wheat leaf rust fungus Puccinia triticina }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:32390310" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a MelampsoraV2
![]() | This area was generated with Dilib version V0.6.38. | ![]() |