Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene
Identifieur interne : 000E47 ( Main/Merge ); précédent : 000E46; suivant : 000E48Transformation of the flax rust fungus, Melampsora lini: selection via silencing of an avirulence gene
Auteurs : Gregory J. Lawrence ; Peter N. Dodds ; Jeffrey G. EllisSource :
- The Plant journal : for cell and molecular biology [ 0960-7412 ] ; 2009.
Descripteurs français
- KwdFr :
- Basidiomycota (génétique), Basidiomycota (pathogénicité), Extinction de l'expression des gènes (MeSH), Génie génétique (méthodes), Lin (génétique), Lin (microbiologie), Maladies des plantes (génétique), Maladies des plantes (microbiologie), Transformation génétique (MeSH), Virulence (génétique), Végétaux génétiquement modifiés (MeSH).
- MESH :
- génétique : Basidiomycota, Lin, Maladies des plantes, Virulence.
- microbiologie : Lin, Maladies des plantes.
- méthodes : Génie génétique.
- pathogénicité : Basidiomycota.
- Extinction de l'expression des gènes, Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- genetics : Basidiomycota, Flax, Plant Diseases, Virulence.
- methods : Genetic Engineering.
- microbiology : Flax, Plant Diseases.
- pathogenicity : Basidiomycota.
- Gene Silencing, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an
Url:
DOI: 10.1111/j.1365-313X.2009.04052.x
PubMed: 19874543
PubMed Central: 3142615
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PMC:3142615Le document en format XML
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: selection via silencing of an avirulence gene</title>
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: selection via silencing of an avirulence gene</title>
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<term>Flax (genetics)</term>
<term>Flax (microbiology)</term>
<term>Gene Silencing (MeSH)</term>
<term>Genetic Engineering (methods)</term>
<term>Plant Diseases (genetics)</term>
<term>Plant Diseases (microbiology)</term>
<term>Plants, Genetically Modified (MeSH)</term>
<term>Transformation, Genetic (MeSH)</term>
<term>Virulence (genetics)</term>
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<term>Basidiomycota (pathogénicité)</term>
<term>Extinction de l'expression des gènes (MeSH)</term>
<term>Génie génétique (méthodes)</term>
<term>Lin (génétique)</term>
<term>Lin (microbiologie)</term>
<term>Maladies des plantes (génétique)</term>
<term>Maladies des plantes (microbiologie)</term>
<term>Transformation génétique (MeSH)</term>
<term>Virulence (génétique)</term>
<term>Végétaux génétiquement modifiés (MeSH)</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Basidiomycota</term>
<term>Flax</term>
<term>Plant Diseases</term>
<term>Virulence</term>
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<term>Transformation, Genetic</term>
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<front><div type="abstract" xml:lang="en"><title>SUMMARY</title>
<p id="P2">Rust fungi cause devastating diseases on many important food crops, with a damaging stem rust epidemic currently affecting wheat production in Africa and the Middle East. These parasitic fungi propagate exclusively on plants, precluding the use of many biotechnological tools available for other culturable fungi. In particular the lack of a stable transformation system has been an impediment to the genetic manipulation required for molecular analysis of rust pathogenicity. We have developed an <italic>Agrobacterium</italic>
-mediated genetic transformation procedure for the model flax rust fungus <italic>Melampsora lini</italic>
, which infects flax (<italic>Linum usitatissimum</italic>
). Selection of transgenic rust lines is based on silencing of <italic>AvrL567</italic>
, which encodes a rust effector protein that is recognised by the flax <italic>L6</italic>
immune receptor. The non-transgenic rust line is unable to infect flax plants expressing <italic>L6</italic>
, while silenced transgenic lines are virulent on these plants, providing an effective selection system. This directly confirms that the cloned <italic>AvrL567</italic>
gene is responsible for flax rust virulence phenotypes, and demonstrates the utility of this system to probe rust gene function.</p>
</div>
</front>
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