A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors
Identifieur interne : 000259 ( Main/Exploration ); précédent : 000258; suivant : 000260A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors
Auteurs : Isabel M. L. Saur [Allemagne] ; Saskia Bauer [Allemagne] ; Xunli Lu [Allemagne, République populaire de Chine] ; Paul Schulze-Lefert [Allemagne]Source :
- Plant Methods [ 1746-4811 ] ; 2019.
Abstract
Plant disease resistance to host-adapted pathogens is often mediated by host nucleotide-binding and leucine-rich repeat (NLR) receptors that detect matching pathogen avirulence effectors (AVR) inside plant cells. AVR-triggered NLR activation is typically associated with a rapid host cell death at sites of attempted infection and this response constitutes a widely used surrogate for NLR activation. However, it is challenging to assess this cell death in cereal hosts.
Here we quantify cell death upon NLR-mediated recognition of fungal pathogen AVRs in mesophyll leaf protoplasts of barley and wheat. We provide measurements for the recognition of the fungal AVRs
Our data demonstrate that the here described approach can be effectively used to detect and quantify death of wheat and barley cells induced by overexpression of
Url:
DOI: 10.1186/s13007-019-0502-0
PubMed: 31666804
PubMed Central: 6813131
Affiliations:
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Saur</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation></author><author><name sortKey="Bauer, Saskia" sort="Bauer, Saskia" uniqKey="Bauer S" first="Saskia" last="Bauer">Saskia Bauer</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Xunli" sort="Lu, Xunli" uniqKey="Lu X" first="Xunli" last="Lu">Xunli Lu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0530 8290</institution-id><institution-id institution-id-type="GRID">grid.22935.3f</institution-id><institution>Present Address: Department of Plant Pathology, College of Plant Protection,</institution><institution>China Agricultural University,</institution></institution-wrap>Beijing, 100193 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.503026.2</institution-id><institution>Cluster of Excellence on Plant Sciences,</institution></institution-wrap>40225 Düsseldorf, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>40225 Düsseldorf</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31666804</idno><idno type="pmc">6813131</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6813131</idno><idno type="RBID">PMC:6813131</idno><idno type="doi">10.1186/s13007-019-0502-0</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000216</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000216</idno><idno type="wicri:Area/Pmc/Curation">000216</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000216</idno><idno type="wicri:Area/Pmc/Checkpoint">000250</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000250</idno><idno type="wicri:Area/Ncbi/Merge">000A86</idno><idno type="wicri:Area/Ncbi/Curation">000A86</idno><idno type="wicri:Area/Ncbi/Checkpoint">000A86</idno><idno type="wicri:Area/Main/Merge">000259</idno><idno type="wicri:Area/Main/Curation">000259</idno><idno type="wicri:Area/Main/Exploration">000259</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A cell death assay in barley and wheat protoplasts for identification and validation of matching pathogen AVR effector and plant NLR immune receptors</title><author><name sortKey="Saur, Isabel M L" sort="Saur, Isabel M L" uniqKey="Saur I" first="Isabel M. L." last="Saur">Isabel M. L. Saur</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation></author><author><name sortKey="Bauer, Saskia" sort="Bauer, Saskia" uniqKey="Bauer S" first="Saskia" last="Bauer">Saskia Bauer</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation></author><author><name sortKey="Lu, Xunli" sort="Lu, Xunli" uniqKey="Lu X" first="Xunli" last="Lu">Xunli Lu</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 0530 8290</institution-id><institution-id institution-id-type="GRID">grid.22935.3f</institution-id><institution>Present Address: Department of Plant Pathology, College of Plant Protection,</institution><institution>China Agricultural University,</institution></institution-wrap>Beijing, 100193 China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing</wicri:regionArea></affiliation></author><author><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0001 0660 6765</institution-id><institution-id institution-id-type="GRID">grid.419498.9</institution-id><institution>Department of Plant-Microbe Interactions,</institution><institution>Max Planck Institute for Plant Breeding Research,</institution></institution-wrap>50829 Cologne, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>50829 Cologne</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.503026.2</institution-id><institution>Cluster of Excellence on Plant Sciences,</institution></institution-wrap>40225 Düsseldorf, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>40225 Düsseldorf</wicri:regionArea></affiliation></author></analytic><series><title level="j">Plant Methods</title><idno type="eISSN">1746-4811</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Plant disease resistance to host-adapted pathogens is often mediated by host nucleotide-binding and leucine-rich repeat (NLR) receptors that detect matching pathogen avirulence effectors (AVR) inside plant cells. AVR-triggered NLR activation is typically associated with a rapid host cell death at sites of attempted infection and this response constitutes a widely used surrogate for NLR activation. However, it is challenging to assess this cell death in cereal hosts.</p></sec><sec><title>Results</title><p id="Par2">Here we quantify cell death upon NLR-mediated recognition of fungal pathogen AVRs in mesophyll leaf protoplasts of barley and wheat. We provide measurements for the recognition of the fungal AVRs <italic>AvrSr50</italic> and <italic>AVR</italic><sub><italic>a1</italic></sub> by their respective cereal NLRs <italic>Sr50</italic> and <italic>Mla1</italic> upon overexpression of the <italic>AVR</italic> and <italic>NLR</italic> pairs in mesophyll protoplast of both, wheat and barley.</p></sec><sec><title>Conclusions</title><p id="Par3">Our data demonstrate that the here described approach can be effectively used to detect and quantify death of wheat and barley cells induced by overexpression of <italic>NLR</italic> and <italic>AVR</italic> effectors or <italic>AVR</italic> effector candidate genes from diverse fungal pathogens within 24 h.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author><author><name sortKey="Rathjen, Jp" uniqKey="Rathjen J">JP Rathjen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maekawa, T" uniqKey="Maekawa T">T Maekawa</name></author><author><name sortKey="Kufer, Ta" uniqKey="Kufer T">TA Kufer</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Biezen, Ea" uniqKey="Van Der Biezen E">EA van der Biezen</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Hoorn, Ral" uniqKey="Van Der Hoorn R">RAL van der Hoorn</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, Wj" uniqKey="Thomas W">WJ Thomas</name></author><author><name sortKey="Thireault, Ca" uniqKey="Thireault C">CA Thireault</name></author><author><name sortKey="Kimbrel, Ja" uniqKey="Kimbrel J">JA Kimbrel</name></author><author><name sortKey="Chang, Jh" uniqKey="Chang J">JH Chang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Upadhyaya, Nm" uniqKey="Upadhyaya N">NM Upadhyaya</name></author><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Upadhyaya, Nm" uniqKey="Upadhyaya N">NM Upadhyaya</name></author><author><name sortKey="Mago, R" uniqKey="Mago R">R Mago</name></author><author><name sortKey="Staskawicz, Bj" uniqKey="Staskawicz B">BJ Staskawicz</name></author><author><name sortKey="Ayliffe, Ma" uniqKey="Ayliffe M">MA Ayliffe</name></author><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, Xl" uniqKey="Lu X">XL Lu</name></author><author><name sortKey="Kracher, B" uniqKey="Kracher B">B Kracher</name></author><author><name sortKey="Saur, Iml" uniqKey="Saur I">IML Saur</name></author><author><name sortKey="Bauer, S" uniqKey="Bauer S">S Bauer</name></author><author><name sortKey="Ellwood, Sr" uniqKey="Ellwood S">SR Ellwood</name></author><author><name sortKey="Wise, R" uniqKey="Wise R">R Wise</name></author><author><name sortKey="Yaeno, T" uniqKey="Yaeno T">T Yaeno</name></author><author><name sortKey="Maekawa, T" uniqKey="Maekawa T">T Maekawa</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author><author><name sortKey="Lawrence, Gj" uniqKey="Lawrence G">GJ Lawrence</name></author><author><name sortKey="Catanzariti, Am" uniqKey="Catanzariti A">AM Catanzariti</name></author><author><name sortKey="Ayliffe, Ma" uniqKey="Ayliffe M">MA Ayliffe</name></author><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, Ws" uniqKey="Lee W">WS Lee</name></author><author><name sortKey="Hammond Kosack, Ke" uniqKey="Hammond Kosack K">KE Hammond-Kosack</name></author><author><name sortKey="Kanyuka, K" uniqKey="Kanyuka K">K Kanyuka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yuan, C" uniqKey="Yuan C">C Yuan</name></author><author><name sortKey="Li, C" uniqKey="Li C">C Li</name></author><author><name sortKey="Yan, L" uniqKey="Yan L">L Yan</name></author><author><name sortKey="Jackson, Ao" uniqKey="Jackson A">AO Jackson</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z Liu</name></author><author><name sortKey="Han, C" uniqKey="Han C">C Han</name></author><author><name sortKey="Yu, J" uniqKey="Yu J">J Yu</name></author><author><name sortKey="Li, D" uniqKey="Li D">D Li</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bouton, C" uniqKey="Bouton C">C Bouton</name></author><author><name sortKey="King, Rc" uniqKey="King R">RC King</name></author><author><name sortKey="Chen, Hx" uniqKey="Chen H">HX Chen</name></author><author><name sortKey="Azhakanandam, K" uniqKey="Azhakanandam K">K Azhakanandam</name></author><author><name sortKey="Bieri, S" uniqKey="Bieri S">S Bieri</name></author><author><name sortKey="Hammond Kosack, Ke" uniqKey="Hammond Kosack K">KE Hammond-Kosack</name></author><author><name sortKey="Kanyuka, K" uniqKey="Kanyuka K">K Kanyuka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cesari, S" uniqKey="Cesari S">S Cesari</name></author><author><name sortKey="Bernoux, M" uniqKey="Bernoux M">M Bernoux</name></author><author><name sortKey="Moncuquet, P" uniqKey="Moncuquet P">P Moncuquet</name></author><author><name sortKey="Kroj, T" uniqKey="Kroj T">T Kroj</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cesari, S" uniqKey="Cesari S">S Cesari</name></author><author><name sortKey="Moore, J" uniqKey="Moore J">J Moore</name></author><author><name sortKey="Chen, Ch" uniqKey="Chen C">CH Chen</name></author><author><name sortKey="Webb, D" uniqKey="Webb D">D Webb</name></author><author><name sortKey="Periyannan, S" uniqKey="Periyannan S">S Periyannan</name></author><author><name sortKey="Mago, R" uniqKey="Mago R">R Mago</name></author><author><name sortKey="Bernoux, M" uniqKey="Bernoux M">M Bernoux</name></author><author><name sortKey="Lagudah, Es" uniqKey="Lagudah E">ES Lagudah</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bai, S" uniqKey="Bai S">S Bai</name></author><author><name sortKey="Liu, J" uniqKey="Liu J">J Liu</name></author><author><name sortKey="Chang, C" uniqKey="Chang C">C Chang</name></author><author><name sortKey="Zhang, L" uniqKey="Zhang L">L Zhang</name></author><author><name sortKey="Maekawa, T" uniqKey="Maekawa T">T Maekawa</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author><author><name sortKey="Xiao, W" uniqKey="Xiao W">W Xiao</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y Liu</name></author><author><name sortKey="Chai, J" uniqKey="Chai J">J Chai</name></author><author><name sortKey="Takken, Fl" uniqKey="Takken F">FL Takken</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author><author><name sortKey="Shen, Qh" uniqKey="Shen Q">QH Shen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bourras, S" uniqKey="Bourras S">S Bourras</name></author><author><name sortKey="Kunz, L" uniqKey="Kunz L">L Kunz</name></author><author><name sortKey="Xue, M" uniqKey="Xue M">M Xue</name></author><author><name sortKey="Praz, Cr" uniqKey="Praz C">CR Praz</name></author><author><name sortKey="Muller, Mc" uniqKey="Muller M">MC Müller</name></author><author><name sortKey="K Lin, C" uniqKey="K Lin C">C Kälin</name></author><author><name sortKey="Schl Fli, M" uniqKey="Schl Fli M">M Schläfli</name></author><author><name sortKey="Ackermann, P" uniqKey="Ackermann P">P Ackermann</name></author><author><name sortKey="Fluckiger, S" uniqKey="Fluckiger S">S Flückiger</name></author><author><name sortKey="Parlange, F" uniqKey="Parlange F">F Parlange</name></author><author><name sortKey="Menardo, F" uniqKey="Menardo F">F Menardo</name></author><author><name sortKey="Schaefer, Lk" uniqKey="Schaefer L">LK Schaefer</name></author><author><name sortKey="Ben David, R" uniqKey="Ben David R">R Ben-David</name></author><author><name sortKey="Roffler, S" uniqKey="Roffler S">S Roffler</name></author><author><name sortKey="Oberhaensli, S" uniqKey="Oberhaensli S">S Oberhaensli</name></author><author><name sortKey="Widrig, V" uniqKey="Widrig V">V Widrig</name></author><author><name sortKey="Lindner, S" uniqKey="Lindner S">S Lindner</name></author><author><name sortKey="Isaksson, J" uniqKey="Isaksson J">J Isaksson</name></author><author><name sortKey="Wicker, T" uniqKey="Wicker T">T Wicker</name></author><author><name sortKey="Yu, Dz" uniqKey="Yu D">DZ Yu</name></author><author><name sortKey="Keller, B" uniqKey="Keller B">B Keller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saur, Im" uniqKey="Saur I">IM Saur</name></author><author><name sortKey="Bauer, S" uniqKey="Bauer S">S Bauer</name></author><author><name sortKey="Kracher, B" uniqKey="Kracher B">B Kracher</name></author><author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name></author><author><name sortKey="Franzeskakis, L" uniqKey="Franzeskakis L">L Franzeskakis</name></author><author><name sortKey="Muller, Mc" uniqKey="Muller M">MC Müller</name></author><author><name sortKey="Sabelleck, B" uniqKey="Sabelleck B">B Sabelleck</name></author><author><name sortKey="Kummel, F" uniqKey="Kummel F">F Kümmel</name></author><author><name sortKey="Panstruga, R" uniqKey="Panstruga R">R Panstruga</name></author><author><name sortKey="Maekawa, T" uniqKey="Maekawa T">T Maekawa</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bourras, S" uniqKey="Bourras S">S Bourras</name></author><author><name sortKey="Mcnally, Ke" uniqKey="Mcnally K">KE McNally</name></author><author><name sortKey="Ben David, R" uniqKey="Ben David R">R Ben-David</name></author><author><name sortKey="Parlange, F" uniqKey="Parlange F">F Parlange</name></author><author><name sortKey="Roffler, S" uniqKey="Roffler S">S Roffler</name></author><author><name sortKey="Praz, Cr" uniqKey="Praz C">CR Praz</name></author><author><name sortKey="Oberhaensli, S" uniqKey="Oberhaensli S">S Oberhaensli</name></author><author><name sortKey="Menardo, F" uniqKey="Menardo F">F Menardo</name></author><author><name sortKey="Stirnweis, D" uniqKey="Stirnweis D">D Stirnweis</name></author><author><name sortKey="Frenkel, Z" uniqKey="Frenkel Z">Z Frenkel</name></author><author><name sortKey="Schaefer, Lk" uniqKey="Schaefer L">LK Schaefer</name></author><author><name sortKey="Fluckiger, S" uniqKey="Fluckiger S">S Fluckiger</name></author><author><name sortKey="Treier, G" uniqKey="Treier G">G Treier</name></author><author><name sortKey="Herren, G" uniqKey="Herren G">G Herren</name></author><author><name sortKey="Korol, Ab" uniqKey="Korol A">AB Korol</name></author><author><name sortKey="Wicker, T" uniqKey="Wicker T">T Wicker</name></author><author><name sortKey="Keller, B" uniqKey="Keller B">B Keller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoo, Sd" uniqKey="Yoo S">SD Yoo</name></author><author><name sortKey="Cho, Yh" uniqKey="Cho Y">YH Cho</name></author><author><name sortKey="Sheen, J" uniqKey="Sheen J">J Sheen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Upadhyaya, Nm" uniqKey="Upadhyaya N">NM Upadhyaya</name></author><author><name sortKey="Ortiz, D" uniqKey="Ortiz D">D Ortiz</name></author><author><name sortKey="Sperschneider, J" uniqKey="Sperschneider J">J Sperschneider</name></author><author><name sortKey="Li, F" uniqKey="Li F">F Li</name></author><author><name sortKey="Bouton, C" uniqKey="Bouton C">C Bouton</name></author><author><name sortKey="Breen, S" uniqKey="Breen S">S Breen</name></author><author><name sortKey="Dong, C" uniqKey="Dong C">C Dong</name></author><author><name sortKey="Xu, B" uniqKey="Xu B">B Xu</name></author><author><name sortKey="Zhang, X" uniqKey="Zhang X">X Zhang</name></author><author><name sortKey="Mago, R" uniqKey="Mago R">R Mago</name></author><author><name sortKey="Newell, K" uniqKey="Newell K">K Newell</name></author><author><name sortKey="Xia, X" uniqKey="Xia X">X Xia</name></author><author><name sortKey="Bernoux, M" uniqKey="Bernoux M">M Bernoux</name></author><author><name sortKey="Taylor, Jm" uniqKey="Taylor J">JM Taylor</name></author><author><name sortKey="Steffenson, B" uniqKey="Steffenson B">B Steffenson</name></author><author><name sortKey="Jin, Y" uniqKey="Jin Y">Y Jin</name></author><author><name sortKey="Zhang, P" uniqKey="Zhang P">P Zhang</name></author><author><name sortKey="Kanyuka, K" uniqKey="Kanyuka K">K Kanyuka</name></author><author><name sortKey="Figueroa, M" uniqKey="Figueroa M">M Figueroa</name></author><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author><author><name sortKey="Park, Rf" uniqKey="Park R">RF Park</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mago, R" uniqKey="Mago R">R Mago</name></author><author><name sortKey="Zhang, P" uniqKey="Zhang P">P Zhang</name></author><author><name sortKey="Vautrin, S" uniqKey="Vautrin S">S Vautrin</name></author><author><name sortKey="Simkova, H" uniqKey="Simkova H">H Šimková</name></author><author><name sortKey="Bansal, U" uniqKey="Bansal U">U Bansal</name></author><author><name sortKey="Luo, Mc" uniqKey="Luo M">MC Luo</name></author><author><name sortKey="Rouse, M" uniqKey="Rouse M">M Rouse</name></author><author><name sortKey="Karaoglu, H" uniqKey="Karaoglu H">H Karaoglu</name></author><author><name sortKey="Periyannan, S" uniqKey="Periyannan S">S Periyannan</name></author><author><name sortKey="Kolmer, J" uniqKey="Kolmer J">J Kolmer</name></author><author><name sortKey="Jin, Y" uniqKey="Jin Y">Y Jin</name></author><author><name sortKey="Ayliffe, Ma" uniqKey="Ayliffe M">MA Ayliffe</name></author><author><name sortKey="Bariana, H" uniqKey="Bariana H">H Bariana</name></author><author><name sortKey="Park, Rf" uniqKey="Park R">RF Park</name></author><author><name sortKey="Mcintosh, R" uniqKey="Mcintosh R">R McIntosh</name></author><author><name sortKey="Dolezel, J" uniqKey="Dolezel J">J Dolezel</name></author><author><name sortKey="Berges, H" uniqKey="Berges H">H Berges</name></author><author><name sortKey="Spielmeyer, W" uniqKey="Spielmeyer W">W Spielmeyer</name></author><author><name sortKey="Lagudah, Es" uniqKey="Lagudah E">ES Lagudah</name></author><author><name sortKey="Ellis, Jg" uniqKey="Ellis J">JG Ellis</name></author><author><name sortKey="Dodds, Pn" uniqKey="Dodds P">PN Dodds</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stirnweis, D" uniqKey="Stirnweis D">D Stirnweis</name></author><author><name sortKey="Milani, Sd" uniqKey="Milani S">SD Milani</name></author><author><name sortKey="Brunner, S" uniqKey="Brunner S">S Brunner</name></author><author><name sortKey="Herren, G" uniqKey="Herren G">G Herren</name></author><author><name sortKey="Buchmann, G" uniqKey="Buchmann G">G Buchmann</name></author><author><name sortKey="Peditto, D" uniqKey="Peditto D">D Peditto</name></author><author><name sortKey="Jordan, T" uniqKey="Jordan T">T Jordan</name></author><author><name sortKey="Keller, B" uniqKey="Keller B">B Keller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hurni, S" uniqKey="Hurni S">S Hurni</name></author><author><name sortKey="Brunner, S" uniqKey="Brunner S">S Brunner</name></author><author><name sortKey="Stirnweis, D" uniqKey="Stirnweis D">D Stirnweis</name></author><author><name sortKey="Herren, G" uniqKey="Herren G">G Herren</name></author><author><name sortKey="Peditto, D" uniqKey="Peditto D">D Peditto</name></author><author><name sortKey="Mcintosh, Ra" uniqKey="Mcintosh R">RA McIntosh</name></author><author><name sortKey="Keller, B" uniqKey="Keller B">B Keller</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tran, Dtn" uniqKey="Tran D">DTN Tran</name></author><author><name sortKey="Chung, Eh" uniqKey="Chung E">EH Chung</name></author><author><name sortKey="Habring Muller, A" uniqKey="Habring Muller A">A Habring-Muller</name></author><author><name sortKey="Demar, M" uniqKey="Demar M">M Demar</name></author><author><name sortKey="Schwab, R" uniqKey="Schwab R">R Schwab</name></author><author><name sortKey="Dangl, Jl" uniqKey="Dangl J">JL Dangl</name></author><author><name sortKey="Weigel, D" uniqKey="Weigel D">D Weigel</name></author><author><name sortKey="Chae, E" uniqKey="Chae E">E Chae</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshida, K" uniqKey="Yoshida K">K Yoshida</name></author><author><name sortKey="Saitoh, H" uniqKey="Saitoh H">H Saitoh</name></author><author><name sortKey="Fujisawa, S" uniqKey="Fujisawa S">S Fujisawa</name></author><author><name sortKey="Kanzaki, H" uniqKey="Kanzaki H">H Kanzaki</name></author><author><name sortKey="Matsumura, H" uniqKey="Matsumura H">H Matsumura</name></author><author><name sortKey="Yoshida, K" uniqKey="Yoshida K">K Yoshida</name></author><author><name sortKey="Tosa, Y" uniqKey="Tosa Y">Y Tosa</name></author><author><name sortKey="Chuma, I" uniqKey="Chuma I">I Chuma</name></author><author><name sortKey="Takano, Y" uniqKey="Takano Y">Y Takano</name></author><author><name sortKey="Win, J" uniqKey="Win J">J Win</name></author><author><name sortKey="Kamoun, S" uniqKey="Kamoun S">S Kamoun</name></author><author><name sortKey="Terauchi, R" uniqKey="Terauchi R">R Terauchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Halterman, Da" uniqKey="Halterman D">DA Halterman</name></author><author><name sortKey="Wei, Fs" uniqKey="Wei F">FS Wei</name></author><author><name sortKey="Wise, Rp" uniqKey="Wise R">RP Wise</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bendahmane, A" uniqKey="Bendahmane A">A Bendahmane</name></author><author><name sortKey="Kanyuka, K" uniqKey="Kanyuka K">K Kanyuka</name></author><author><name sortKey="Baulcombe, Dc" uniqKey="Baulcombe D">DC Baulcombe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sarris, Pf" uniqKey="Sarris P">PF Sarris</name></author><author><name sortKey="Duxbury, Z" uniqKey="Duxbury Z">Z Duxbury</name></author><author><name sortKey="Huh, Su" uniqKey="Huh S">SU Huh</name></author><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y Ma</name></author><author><name sortKey="Segonzac, C" uniqKey="Segonzac C">C Segonzac</name></author><author><name sortKey="Sklenar, J" uniqKey="Sklenar J">J Sklenar</name></author><author><name sortKey="Derbyshire, P" uniqKey="Derbyshire P">P Derbyshire</name></author><author><name sortKey="Cevik, V" uniqKey="Cevik V">V Cevik</name></author><author><name sortKey="Rallapalli, G" uniqKey="Rallapalli G">G Rallapalli</name></author><author><name sortKey="Saucet, Sb" uniqKey="Saucet S">SB Saucet</name></author><author><name sortKey="Wirthmueller, L" uniqKey="Wirthmueller L">L Wirthmueller</name></author><author><name sortKey="Menke, Flh" uniqKey="Menke F">FLH Menke</name></author><author><name sortKey="Sohn, Kh" uniqKey="Sohn K">KH Sohn</name></author><author><name sortKey="Jones, Jdg" uniqKey="Jones J">JDG Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, Ic" uniqKey="Yu I">IC Yu</name></author><author><name sortKey="Parker, J" uniqKey="Parker J">J Parker</name></author><author><name sortKey="Bent, Af" uniqKey="Bent A">AF Bent</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bulgarelli, D" uniqKey="Bulgarelli D">D Bulgarelli</name></author><author><name sortKey="Biselli, C" uniqKey="Biselli C">C Biselli</name></author><author><name sortKey="Collins, Nc" uniqKey="Collins N">NC Collins</name></author><author><name sortKey="Consonni, G" uniqKey="Consonni G">G Consonni</name></author><author><name sortKey="Stanca, Am" uniqKey="Stanca A">AM Stanca</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author><author><name sortKey="Vale, G" uniqKey="Vale G">G Valè</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Himmelbach, A" uniqKey="Himmelbach A">A Himmelbach</name></author><author><name sortKey="Zierold, U" uniqKey="Zierold U">U Zierold</name></author><author><name sortKey="Hensel, G" uniqKey="Hensel G">G Hensel</name></author><author><name sortKey="Riechen, J" uniqKey="Riechen J">J Riechen</name></author><author><name sortKey="Douchkov, D" uniqKey="Douchkov D">D Douchkov</name></author><author><name sortKey="Schweizer, P" uniqKey="Schweizer P">P Schweizer</name></author><author><name sortKey="Kumlehn, J" uniqKey="Kumlehn J">J Kumlehn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Christensen, Ah" uniqKey="Christensen A">AH Christensen</name></author><author><name sortKey="Quail, Ph" uniqKey="Quail P">PH Quail</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muller, J" uniqKey="Muller J">J Muller</name></author><author><name sortKey="Piffanelli, P" uniqKey="Piffanelli P">P Piffanelli</name></author><author><name sortKey="Devoto, A" uniqKey="Devoto A">A Devoto</name></author><author><name sortKey="Miklis, M" uniqKey="Miklis M">M Miklis</name></author><author><name sortKey="Elliott, C" uniqKey="Elliott C">C Elliott</name></author><author><name sortKey="Ortmann, B" uniqKey="Ortmann B">B Ortmann</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author><author><name sortKey="Panstruga, R" uniqKey="Panstruga R">R Panstruga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bhat, Ra" uniqKey="Bhat R">RA Bhat</name></author><author><name sortKey="Miklis, M" uniqKey="Miklis M">M Miklis</name></author><author><name sortKey="Schmelzer, E" uniqKey="Schmelzer E">E Schmelzer</name></author><author><name sortKey="Schulze Lefert, P" uniqKey="Schulze Lefert P">P Schulze-Lefert</name></author><author><name sortKey="Panstruga, R" uniqKey="Panstruga R">R Panstruga</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li><li>République populaire de Chine</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Saur, Isabel M L" sort="Saur, Isabel M L" uniqKey="Saur I" first="Isabel M. L." last="Saur">Isabel M. L. Saur</name></noRegion><name sortKey="Bauer, Saskia" sort="Bauer, Saskia" uniqKey="Bauer S" first="Saskia" last="Bauer">Saskia Bauer</name><name sortKey="Lu, Xunli" sort="Lu, Xunli" uniqKey="Lu X" first="Xunli" last="Lu">Xunli Lu</name><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name><name sortKey="Schulze Lefert, Paul" sort="Schulze Lefert, Paul" uniqKey="Schulze Lefert P" first="Paul" last="Schulze-Lefert">Paul Schulze-Lefert</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Xunli" sort="Lu, Xunli" uniqKey="Lu X" first="Xunli" last="Lu">Xunli Lu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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