GrapevineDiseaseV1, Main, Exploration, bibRecord, 000606

Virus-derived gene expression and RNA interference vector for grapevine.

Identifieur interne : 000606 ( Main/Exploration ); précédent : 000605; suivant : 000607

Virus-derived gene expression and RNA interference vector for grapevine.

Auteurs : Elizabeth G. Kurth [États-Unis] ; Valera V. Peremyslov ; Alexey I. Prokhnevsky ; Kristin D. Kasschau ; Marilyn Miller ; James C. Carrington ; Valerian V. Dolja

Source :

Journal of virology [ 1098-5514 ] ; 2012.

RBID : pubmed:22438553

Descripteurs français

KwdFr :
- Analyse de séquence d'ADN (MeSH), Closteroviridae (génétique), Données de séquences moléculaires (MeSH), Expression des gènes (MeSH), Interférence par ARN (MeSH), Métabolisme glucidique (MeSH), Protéines végétales (biosynthèse), Régulation de l'expression des gènes (MeSH), Vecteurs génétiques (MeSH), Vitis (génétique), Vitis (métabolisme), Vitis (virologie).
MESH :
- biosynthèse : Protéines végétales.
- génétique : Closteroviridae, Vitis.
- métabolisme : Vitis.
- virologie : Vitis.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Expression des gènes, Interférence par ARN, Métabolisme glucidique, Régulation de l'expression des gènes, Vecteurs génétiques.

English descriptors

KwdEn :
- Carbohydrate Metabolism (MeSH), Closteroviridae (genetics), Gene Expression (MeSH), Gene Expression Regulation (MeSH), Genetic Vectors (MeSH), Molecular Sequence Data (MeSH), Plant Proteins (biosynthesis), RNA Interference (MeSH), Sequence Analysis, DNA (MeSH), Vitis (genetics), Vitis (metabolism), Vitis (virology).
MESH :
- chemical , biosynthesis : Plant Proteins.
- genetics : Closteroviridae, Vitis.
- metabolism : Vitis.
- virology : Vitis.
- Carbohydrate Metabolism, Gene Expression, Gene Expression Regulation, Genetic Vectors, Molecular Sequence Data, RNA Interference, Sequence Analysis, DNA.

Abstract

The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.

DOI: 10.1128/JVI.00436-12
PubMed: 22438553
PubMed Central: PMC3372183

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000643
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Le document en format XML

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<front><div type="abstract" xml:lang="en">The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction of desired traits into grapevine without heritable modifications to the genome. This vector expresses recombinant proteins in the phloem tissue that is involved in sugar transport throughout the plant, from leaves to roots to berries. Furthermore, the vector provides a powerful RNA interference (RNAi) capability of regulating the expression of endogenous genes via virus-induced gene-silencing (VIGS) technology. Additional advantages of this vector include superb genetic capacity and stability, as well as the swiftness of technology implementation. The most significant applications of the viral vector include functional genomics of the grapevine and disease control via RNAi-enabled vaccination against pathogens or invertebrate pests.</div>
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	Serveur d'exploration sur les maladies des plantes grimpantes
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Virus-derived gene expression and RNA interference vector for grapevine.

Virus-derived gene expression and RNA interference vector for grapevine.

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