Development of infectious cDNA clones of Grapevine leafroll-associated virus 3 and analyses of the 5' non-translated region for replication and virion formation.
Identifieur interne : 000268 ( Main/Exploration ); précédent : 000267; suivant : 000269Development of infectious cDNA clones of Grapevine leafroll-associated virus 3 and analyses of the 5' non-translated region for replication and virion formation.
Auteurs : Sridhar Jarugula [États-Unis] ; Siddarame Gowda [États-Unis] ; William O. Dawson [États-Unis] ; Rayapati A. Naidu [États-Unis]Source :
- Virology [ 1096-0341 ] ; 2018.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (métabolisme), ARN viral (génétique), ARN viral (métabolisme), Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Clones cellulaires (MeSH), Closteroviridae (génétique), Closteroviridae (métabolisme), Closteroviridae (pathogénicité), Feuilles de plante (virologie), Maladies des plantes (virologie), Mutation (MeSH), Plasmides (composition chimique), Plasmides (métabolisme), Protoplastes (virologie), Régions 5' non traduites (MeSH), Réplication virale (MeSH), Tabac (virologie), Transformation génétique (MeSH), Virion (génétique), Virion (métabolisme), Virion (pathogénicité), Vitis (virologie), Végétaux génétiquement modifiés (virologie).
- MESH :
- composition chimique : Plasmides.
- génétique : ADN complémentaire, ARN viral, Agrobacterium tumefaciens, Closteroviridae, Virion.
- métabolisme : ADN complémentaire, ARN viral, Agrobacterium tumefaciens, Closteroviridae, Plasmides, Virion.
- pathogénicité : Closteroviridae, Virion.
- virologie : Feuilles de plante, Maladies des plantes, Protoplastes, Tabac, Vitis, Végétaux génétiquement modifiés.
- Clones cellulaires, Mutation, Régions 5' non traduites, Réplication virale, Transformation génétique.
English descriptors
- KwdEn :
- 5' Untranslated Regions (MeSH), Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Clone Cells (MeSH), Closteroviridae (genetics), Closteroviridae (metabolism), Closteroviridae (pathogenicity), DNA, Complementary (genetics), DNA, Complementary (metabolism), Mutation (MeSH), Plant Diseases (virology), Plant Leaves (virology), Plants, Genetically Modified (virology), Plasmids (chemistry), Plasmids (metabolism), Protoplasts (virology), RNA, Viral (genetics), RNA, Viral (metabolism), Tobacco (virology), Transformation, Genetic (MeSH), Virion (genetics), Virion (metabolism), Virion (pathogenicity), Virus Replication (MeSH), Vitis (virology).
- MESH :
- chemical , genetics : DNA, Complementary, RNA, Viral.
- chemical , metabolism : DNA, Complementary, RNA, Viral.
- chemical : 5' Untranslated Regions.
- chemistry : Plasmids.
- genetics : Agrobacterium tumefaciens, Closteroviridae, Virion.
- metabolism : Agrobacterium tumefaciens, Closteroviridae, Plasmids, Virion.
- pathogenicity : Closteroviridae, Virion.
- virology : Plant Diseases, Plant Leaves, Plants, Genetically Modified, Protoplasts, Tobacco, Vitis.
- Clone Cells, Mutation, Transformation, Genetic, Virus Replication.
Abstract
Infectious cDNA clones were developed for Grapevine leafroll-associated virus 3 (GLRaV-3, genus Ampelovirus, family Closteroviridae). In vitro RNA transcripts generated from cDNA clones showed replication via the production of 3'-coterminal subgenomic (sg) mRNAs in Nicotiana benthamiana protoplasts. The detection of sgRNAs and the recovery of progeny recombinant virions from N. benthamiana leaves agroinfiltrated with full-length cDNA clones confirmed RNA replication and virion formation. The 5' non-translated region (5' NTR) of GLRaV-3 was exchangeable between genetic variants and complement the corresponding cognate RNA functions in trans. Mutational analysis of the 5' NTR in minireplicon cDNA clones showed that the conserved 40 nucleotides at the 5'-terminus were indispensable for replication, compared to downstream variable portion of the 5' NTR. Some of the functional mutations in the 5' NTR were tolerated in full-length cDNA clones and produced sgRNAs and virions in N. benthamiana leaves, whereas other mutations affected replication and virion formation.
DOI: 10.1016/j.virol.2018.07.023
PubMed: 30103103
Affiliations:
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(metabolism)</term><term>Mutation (MeSH)</term><term>Plant Diseases (virology)</term><term>Plant Leaves (virology)</term><term>Plants, Genetically Modified (virology)</term><term>Plasmids (chemistry)</term><term>Plasmids (metabolism)</term><term>Protoplasts (virology)</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Tobacco (virology)</term><term>Transformation, Genetic (MeSH)</term><term>Virion (genetics)</term><term>Virion (metabolism)</term><term>Virion (pathogenicity)</term><term>Virus Replication (MeSH)</term><term>Vitis (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (métabolisme)</term><term>ARN viral (génétique)</term><term>ARN viral (métabolisme)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (métabolisme)</term><term>Clones cellulaires (MeSH)</term><term>Closteroviridae (génétique)</term><term>Closteroviridae (métabolisme)</term><term>Closteroviridae (pathogénicité)</term><term>Feuilles de plante (virologie)</term><term>Maladies des plantes (virologie)</term><term>Mutation (MeSH)</term><term>Plasmides (composition chimique)</term><term>Plasmides (métabolisme)</term><term>Protoplastes (virologie)</term><term>Régions 5' non traduites (MeSH)</term><term>Réplication virale (MeSH)</term><term>Tabac (virologie)</term><term>Transformation génétique (MeSH)</term><term>Virion (génétique)</term><term>Virion (métabolisme)</term><term>Virion (pathogénicité)</term><term>Vitis (virologie)</term><term>Végétaux génétiquement modifiés (virologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Complementary</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>5' Untranslated Regions</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Plasmides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Closteroviridae</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ARN viral</term><term>Agrobacterium tumefaciens</term><term>Closteroviridae</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Closteroviridae</term><term>Plasmids</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN complémentaire</term><term>ARN viral</term><term>Agrobacterium tumefaciens</term><term>Closteroviridae</term><term>Plasmides</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Closteroviridae</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Closteroviridae</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Feuilles de plante</term><term>Maladies des plantes</term><term>Protoplastes</term><term>Tabac</term><term>Vitis</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Protoplasts</term><term>Tobacco</term><term>Vitis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term><term>Mutation</term><term>Transformation, Genetic</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clones cellulaires</term><term>Mutation</term><term>Régions 5' non traduites</term><term>Réplication virale</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infectious cDNA clones were developed for Grapevine leafroll-associated virus 3 (GLRaV-3, genus Ampelovirus, family Closteroviridae). In vitro RNA transcripts generated from cDNA clones showed replication via the production of 3'-coterminal subgenomic (sg) mRNAs in Nicotiana benthamiana protoplasts. The detection of sgRNAs and the recovery of progeny recombinant virions from N. benthamiana leaves agroinfiltrated with full-length cDNA clones confirmed RNA replication and virion formation. The 5' non-translated region (5' NTR) of GLRaV-3 was exchangeable between genetic variants and complement the corresponding cognate RNA functions in trans. Mutational analysis of the 5' NTR in minireplicon cDNA clones showed that the conserved 40 nucleotides at the 5'-terminus were indispensable for replication, compared to downstream variable portion of the 5' NTR. Some of the functional mutations in the 5' NTR were tolerated in full-length cDNA clones and produced sgRNAs and virions in N. benthamiana leaves, whereas other mutations affected replication and virion formation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30103103</PMID><DateCompleted><Year>2018</Year><Month>09</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>09</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0341</ISSN><JournalIssue CitedMedium="Internet"><Volume>523</Volume><PubDate><Year>2018</Year><Month>10</Month></PubDate></JournalIssue><Title>Virology</Title><ISOAbbreviation>Virology</ISOAbbreviation></Journal><ArticleTitle>Development of infectious cDNA clones of Grapevine leafroll-associated virus 3 and analyses of the 5' non-translated region for replication and virion formation.</ArticleTitle><Pagination><MedlinePgn>89-99</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0042-6822(18)30225-3</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.virol.2018.07.023</ELocationID><Abstract><AbstractText>Infectious cDNA clones were developed for Grapevine leafroll-associated virus 3 (GLRaV-3, genus Ampelovirus, family Closteroviridae). In vitro RNA transcripts generated from cDNA clones showed replication via the production of 3'-coterminal subgenomic (sg) mRNAs in Nicotiana benthamiana protoplasts. The detection of sgRNAs and the recovery of progeny recombinant virions from N. benthamiana leaves agroinfiltrated with full-length cDNA clones confirmed RNA replication and virion formation. The 5' non-translated region (5' NTR) of GLRaV-3 was exchangeable between genetic variants and complement the corresponding cognate RNA functions in trans. Mutational analysis of the 5' NTR in minireplicon cDNA clones showed that the conserved 40 nucleotides at the 5'-terminus were indispensable for replication, compared to downstream variable portion of the 5' NTR. Some of the functional mutations in the 5' NTR were tolerated in full-length cDNA clones and produced sgRNAs and virions in N. benthamiana leaves, whereas other mutations affected replication and virion formation.</AbstractText><CopyrightInformation>Copyright © 2018 Elsevier Inc. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jarugula</LastName><ForeName>Sridhar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, WA 99350, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gowda</LastName><ForeName>Siddarame</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dawson</LastName><ForeName>William O</ForeName><Initials>WO</Initials><AffiliationInfo><Affiliation>Citrus Research and Education Center, University of Florida, Lake Alfred, FL, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Naidu</LastName><ForeName>Rayapati A</ForeName><Initials>RA</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, WA 99350, United States. Electronic address: naidu.rayapati@wsu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>08</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Virology</MedlineTA><NlmUniqueID>0110674</NlmUniqueID><ISSNLinking>0042-6822</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020121">5' Untranslated Regions</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><SupplMeshList><SupplMeshName Type="Organism" UI="C000623917">Grapevine leafroll-associated virus 3</SupplMeshName></SupplMeshList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D020121" MajorTopicYN="N">5' Untranslated Regions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002999" MajorTopicYN="N">Clone Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029381" MajorTopicYN="N">Closteroviridae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011523" MajorTopicYN="N">Protoplasts</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014771" MajorTopicYN="N">Virion</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Ampelovirus</Keyword><Keyword MajorTopicYN="Y">Closteroviridae</Keyword><Keyword MajorTopicYN="Y">Grapevine leafroll disease</Keyword><Keyword MajorTopicYN="Y">Grapevine leafroll-associated virus 3</Keyword><Keyword MajorTopicYN="Y">Infectious cDNA clone</Keyword><Keyword MajorTopicYN="Y">Vitis vinifera</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>05</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>9</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>8</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30103103</ArticleId><ArticleId IdType="pii">S0042-6822(18)30225-3</ArticleId><ArticleId IdType="doi">10.1016/j.virol.2018.07.023</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li><li>Washington (État)</li></region></list><tree><country name="États-Unis"><region name="Washington (État)"><name sortKey="Jarugula, Sridhar" sort="Jarugula, Sridhar" uniqKey="Jarugula S" first="Sridhar" last="Jarugula">Sridhar Jarugula</name></region><name sortKey="Dawson, William O" sort="Dawson, William O" uniqKey="Dawson W" first="William O" last="Dawson">William O. Dawson</name><name sortKey="Gowda, Siddarame" sort="Gowda, Siddarame" uniqKey="Gowda S" first="Siddarame" last="Gowda">Siddarame Gowda</name><name sortKey="Naidu, Rayapati A" sort="Naidu, Rayapati A" uniqKey="Naidu R" first="Rayapati A" last="Naidu">Rayapati A. Naidu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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