[Purification, serology and detection of grapevine leaf roll virus].
Identifieur interne : 000980 ( Main/Corpus ); précédent : 000979; suivant : 000981[Purification, serology and detection of grapevine leaf roll virus].
Auteurs : W. Cai ; S. Xu ; K. Mang ; B. Meng ; Y. MaSource :
- Wei sheng wu xue bao = Acta microbiologica Sinica [ 0001-6209 ] ; 1997.
Abstract
Closterovirus-like particles associated with grapevine leaf roll disease were purified from stem phloem tissue by differential centrifugation and cesium sulphate-sucrose density gradient centrifugation. The particles were between 660-2000 nm long. Most of them were about 1400 nm long and also decorated by the antiserum against GLRV NY-1 isolate. The purified preparation was tested for their serological relatedness in indirect ELISA. The results indicated that these closterovirus-like particles reacted with serotype II, III and IV of GLRV. The antiserum to NY-1 (type III) reacted more strongly than type IV (to VA-4) and type II. (to 1800 nm). In sodium dodecyl sulfate immunodiffusion test, the extract of diseased petiole reacted with serotype III, IV and II. It is possible to be infected by 2 or 3 closterovirus-like particles in China. We have made antiserum to the purified closterovirus-like GLRV and set up a PAS-ELISA to detect GLRV-free grapevine plantelets. We have obtaimed 21 varieties of GLRV-free and GFLV-free grapevine. They shown high quality and quantity in field test.
PubMed: 11189365
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Ma</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:11189365</idno><idno type="pmid">11189365</idno><idno type="wicri:Area/Main/Corpus">000980</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000980</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Purification, serology and detection of grapevine leaf roll virus].</title><author><name sortKey="Cai, W" sort="Cai, W" uniqKey="Cai W" first="W" last="Cai">W. Cai</name><affiliation><nlm:affiliation>Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080.</nlm:affiliation></affiliation></author><author><name sortKey="Xu, S" sort="Xu, S" uniqKey="Xu S" first="S" last="Xu">S. Xu</name></author><author><name sortKey="Mang, K" sort="Mang, K" uniqKey="Mang K" first="K" last="Mang">K. Mang</name></author><author><name sortKey="Meng, B" sort="Meng, B" uniqKey="Meng B" first="B" last="Meng">B. Meng</name></author><author><name sortKey="Ma, Y" sort="Ma, Y" uniqKey="Ma Y" first="Y" last="Ma">Y. Ma</name></author></analytic><series><title level="j">Wei sheng wu xue bao = Acta microbiologica Sinica</title><idno type="ISSN">0001-6209</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Closterovirus-like particles associated with grapevine leaf roll disease were purified from stem phloem tissue by differential centrifugation and cesium sulphate-sucrose density gradient centrifugation. The particles were between 660-2000 nm long. Most of them were about 1400 nm long and also decorated by the antiserum against GLRV NY-1 isolate. The purified preparation was tested for their serological relatedness in indirect ELISA. The results indicated that these closterovirus-like particles reacted with serotype II, III and IV of GLRV. The antiserum to NY-1 (type III) reacted more strongly than type IV (to VA-4) and type II. (to 1800 nm). In sodium dodecyl sulfate immunodiffusion test, the extract of diseased petiole reacted with serotype III, IV and II. It is possible to be infected by 2 or 3 closterovirus-like particles in China. We have made antiserum to the purified closterovirus-like GLRV and set up a PAS-ELISA to detect GLRV-free grapevine plantelets. We have obtaimed 21 varieties of GLRV-free and GFLV-free grapevine. They shown high quality and quantity in field test.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">11189365</PMID><DateCompleted><Year>2001</Year><Month>03</Month><Day>01</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0001-6209</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>5</Issue><PubDate><Year>1997</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Wei sheng wu xue bao = Acta microbiologica Sinica</Title><ISOAbbreviation>Wei Sheng Wu Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Purification, serology and detection of grapevine leaf roll virus].</ArticleTitle><Pagination><MedlinePgn>385-92</MedlinePgn></Pagination><Abstract><AbstractText>Closterovirus-like particles associated with grapevine leaf roll disease were purified from stem phloem tissue by differential centrifugation and cesium sulphate-sucrose density gradient centrifugation. The particles were between 660-2000 nm long. Most of them were about 1400 nm long and also decorated by the antiserum against GLRV NY-1 isolate. The purified preparation was tested for their serological relatedness in indirect ELISA. The results indicated that these closterovirus-like particles reacted with serotype II, III and IV of GLRV. The antiserum to NY-1 (type III) reacted more strongly than type IV (to VA-4) and type II. (to 1800 nm). In sodium dodecyl sulfate immunodiffusion test, the extract of diseased petiole reacted with serotype III, IV and II. It is possible to be infected by 2 or 3 closterovirus-like particles in China. We have made antiserum to the purified closterovirus-like GLRV and set up a PAS-ELISA to detect GLRV-free grapevine plantelets. We have obtaimed 21 varieties of GLRV-free and GFLV-free grapevine. They shown high quality and quantity in field test.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>W</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Institute of Microbiology, The Chinese Academy of Sciences, Beijing 100080.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Mang</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Meng</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Wei Sheng Wu Xue Bao</MedlineTA><NlmUniqueID>21610860R</NlmUniqueID><ISSNLinking>0001-6209</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2001</Year><Month>2</Month><Day>24</Day><Hour>12</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">11189365</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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