First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.
Identifieur interne : 000679 ( Main/Corpus ); précédent : 000678; suivant : 000680First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.
Auteurs : J-Y Yan ; Y-L Peng ; Y. Xie ; X-H Li ; S-W Yao ; M-L Tang ; Z-Y WangSource :
- Plant disease [ 0191-2917 ] ; 2011.
Abstract
In September 2010, grapevine (Vitis vinifera) trunk diseases were observed in several vineyards of Yantai District in Shandong Provinces and Changli County of Hebei Provinces of China. Characteristic symptoms of Botryosphaeria canker were apparent, including dark brown discoloration on the trunk (visible in cross-section), cob base shriveling, drying of fruit clusters, and berry falling (2). To identify the causal pathogen, culturing of fungi was attempted from 387 small pieces of tissue from the canker margins of 43 diseased plants. Samples were surface disinfected by placing them in 75% ethanol for 1 min and rinsing with sterilized water three times before culturing on potato dextrose agar (PDA) at 28°C for 7 to 10 days. Fungi isolated were single spored to obtain pure cultures. On the basis of colony characteristics on PDA, 18 isolates from the 387 tissue pieces were eventually identified as Botryosphaeria obtusa (1), Most of the other fungi isolated were B. dothidea. B. obtusa colonies were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 7 days. Conidia measured 8 to 11 × 17 to 26 μm (n= 50). Two isolates were used for rDNA internal transcribed spacer (ITS) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified for legation with PMD-18T (Takara Company, Dalian, China) vector for sequencing. BLAST searches of two ITS sequences had 99 to 100% identity to B. obtusa. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the greenhouse on grape shoots inoculated with two isolates of B. obtusa originally isolated from diseased plants in the field. Inoculations were made on green shoots of V. vinifera cv. Dunkelfelder T. Six shoots were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep) and placing a colonized agar plug from a 5-day-old culture on the wound and wrapping it with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the dark under moist conditions in the laboratory for 8 to 10 days at 25°C. Inoculated shoots had necrotic cankers after 8 to 10 days and B. obtusa was recovered from each canker margin. The results indicated that some grapevines in China with symptoms of Botryosphaeria canker were infected by B. obtusa. To our knowledge, this is the first report of this pathogen causing trunk disease on grapevine in China. References: (1) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (2) J. R. Úrbez-Torres et al. Plant Dis. 92:519, 2008. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.
DOI: 10.1094/PDIS-11-10-0821
PubMed: 30731973
Links to Exploration step
pubmed:30731973Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.</title><author><name sortKey="Yan, J Y" sort="Yan, J Y" uniqKey="Yan J" first="J-Y" last="Yan">J-Y Yan</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, Y L" sort="Peng, Y L" uniqKey="Peng Y" first="Y-L" last="Peng">Y-L Peng</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Y" sort="Xie, Y" uniqKey="Xie Y" first="Y" last="Xie">Y. Xie</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, X H" sort="Li, X H" uniqKey="Li X" first="X-H" last="Li">X-H Li</name><affiliation><nlm:affiliation>Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yao, S W" sort="Yao, S W" uniqKey="Yao S" first="S-W" last="Yao">S-W Yao</name><affiliation><nlm:affiliation>Laboratory of Plant Resource Utilization, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tang, M L" sort="Tang, M L" uniqKey="Tang M" first="M-L" last="Tang">M-L Tang</name><affiliation><nlm:affiliation>Yantai Agricultural Science and Technology Institute, Yantai, 265500, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Z Y" sort="Wang, Z Y" uniqKey="Wang Z" first="Z-Y" last="Wang">Z-Y Wang</name><affiliation><nlm:affiliation>Institute of Plant Protection, Chinese Academy of Agricultural, Beijing 100193, China. The research was supported by the earmarked fund for Modern Agro-Industry Technology Research System (nycytx-30).</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:30731973</idno><idno type="pmid">30731973</idno><idno type="doi">10.1094/PDIS-11-10-0821</idno><idno type="wicri:Area/Main/Corpus">000679</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000679</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.</title><author><name sortKey="Yan, J Y" sort="Yan, J Y" uniqKey="Yan J" first="J-Y" last="Yan">J-Y Yan</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Peng, Y L" sort="Peng, Y L" uniqKey="Peng Y" first="Y-L" last="Peng">Y-L Peng</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Xie, Y" sort="Xie, Y" uniqKey="Xie Y" first="Y" last="Xie">Y. Xie</name><affiliation><nlm:affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</nlm:affiliation></affiliation></author><author><name sortKey="Li, X H" sort="Li, X H" uniqKey="Li X" first="X-H" last="Li">X-H Li</name><affiliation><nlm:affiliation>Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yao, S W" sort="Yao, S W" uniqKey="Yao S" first="S-W" last="Yao">S-W Yao</name><affiliation><nlm:affiliation>Laboratory of Plant Resource Utilization, Hunan Agricultural University, Changsha, China.</nlm:affiliation></affiliation></author><author><name sortKey="Tang, M L" sort="Tang, M L" uniqKey="Tang M" first="M-L" last="Tang">M-L Tang</name><affiliation><nlm:affiliation>Yantai Agricultural Science and Technology Institute, Yantai, 265500, China.</nlm:affiliation></affiliation></author><author><name sortKey="Wang, Z Y" sort="Wang, Z Y" uniqKey="Wang Z" first="Z-Y" last="Wang">Z-Y Wang</name><affiliation><nlm:affiliation>Institute of Plant Protection, Chinese Academy of Agricultural, Beijing 100193, China. The research was supported by the earmarked fund for Modern Agro-Industry Technology Research System (nycytx-30).</nlm:affiliation></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In September 2010, grapevine (Vitis vinifera) trunk diseases were observed in several vineyards of Yantai District in Shandong Provinces and Changli County of Hebei Provinces of China. Characteristic symptoms of Botryosphaeria canker were apparent, including dark brown discoloration on the trunk (visible in cross-section), cob base shriveling, drying of fruit clusters, and berry falling (2). To identify the causal pathogen, culturing of fungi was attempted from 387 small pieces of tissue from the canker margins of 43 diseased plants. Samples were surface disinfected by placing them in 75% ethanol for 1 min and rinsing with sterilized water three times before culturing on potato dextrose agar (PDA) at 28°C for 7 to 10 days. Fungi isolated were single spored to obtain pure cultures. On the basis of colony characteristics on PDA, 18 isolates from the 387 tissue pieces were eventually identified as Botryosphaeria obtusa (1), Most of the other fungi isolated were B. dothidea. B. obtusa colonies were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 7 days. Conidia measured 8 to 11 × 17 to 26 μm (n= 50). Two isolates were used for rDNA internal transcribed spacer (ITS) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified for legation with PMD-18T (Takara Company, Dalian, China) vector for sequencing. BLAST searches of two ITS sequences had 99 to 100% identity to B. obtusa. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the greenhouse on grape shoots inoculated with two isolates of B. obtusa originally isolated from diseased plants in the field. Inoculations were made on green shoots of V. vinifera cv. Dunkelfelder T. Six shoots were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep) and placing a colonized agar plug from a 5-day-old culture on the wound and wrapping it with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the dark under moist conditions in the laboratory for 8 to 10 days at 25°C. Inoculated shoots had necrotic cankers after 8 to 10 days and B. obtusa was recovered from each canker margin. The results indicated that some grapevines in China with symptoms of Botryosphaeria canker were infected by B. obtusa. To our knowledge, this is the first report of this pathogen causing trunk disease on grapevine in China. References: (1) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (2) J. R. Úrbez-Torres et al. Plant Dis. 92:519, 2008. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30731973</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>95</Volume><Issue>5</Issue><PubDate><Year>2011</Year><Month>May</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.</ArticleTitle><Pagination><MedlinePgn>616</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-11-10-0821</ELocationID><Abstract><AbstractText>In September 2010, grapevine (Vitis vinifera) trunk diseases were observed in several vineyards of Yantai District in Shandong Provinces and Changli County of Hebei Provinces of China. Characteristic symptoms of Botryosphaeria canker were apparent, including dark brown discoloration on the trunk (visible in cross-section), cob base shriveling, drying of fruit clusters, and berry falling (2). To identify the causal pathogen, culturing of fungi was attempted from 387 small pieces of tissue from the canker margins of 43 diseased plants. Samples were surface disinfected by placing them in 75% ethanol for 1 min and rinsing with sterilized water three times before culturing on potato dextrose agar (PDA) at 28°C for 7 to 10 days. Fungi isolated were single spored to obtain pure cultures. On the basis of colony characteristics on PDA, 18 isolates from the 387 tissue pieces were eventually identified as Botryosphaeria obtusa (1), Most of the other fungi isolated were B. dothidea. B. obtusa colonies were grayish white, becoming dark brown with age, and pycnidia were formed after incubation for approximately 7 days. Conidia measured 8 to 11 × 17 to 26 μm (n= 50). Two isolates were used for rDNA internal transcribed spacer (ITS) sequence analysis with primers ITS1 and ITS4 (3). PCR products were separated by electrophoresis and bands were purified for legation with PMD-18T (Takara Company, Dalian, China) vector for sequencing. BLAST searches of two ITS sequences had 99 to 100% identity to B. obtusa. EF1-α and β-tubulin sequence analysis gave similar results. Koch's postulates were completed in the greenhouse on grape shoots inoculated with two isolates of B. obtusa originally isolated from diseased plants in the field. Inoculations were made on green shoots of V. vinifera cv. Dunkelfelder T. Six shoots were inoculated per isolate by wounding with a 4-mm cork borer (2 mm deep) and placing a colonized agar plug from a 5-day-old culture on the wound and wrapping it with Parafilm. Controls were mock inoculated with an agar plug from sterile PDA. Inoculated shoots were incubated in the dark under moist conditions in the laboratory for 8 to 10 days at 25°C. Inoculated shoots had necrotic cankers after 8 to 10 days and B. obtusa was recovered from each canker margin. The results indicated that some grapevines in China with symptoms of Botryosphaeria canker were infected by B. obtusa. To our knowledge, this is the first report of this pathogen causing trunk disease on grapevine in China. References: (1) A. Taylor et al. Australas. Plant Pathol. 34:187, 2005. (2) J. R. Úrbez-Torres et al. Plant Dis. 92:519, 2008. (3) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, 1990.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>J-Y</ForeName><Initials>JY</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Y-L</ForeName><Initials>YL</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Y</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, China Agricultural University, Beijing 100193, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>X-H</ForeName><Initials>XH</Initials><AffiliationInfo><Affiliation>Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Science, Beijing 100097, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yao</LastName><ForeName>S-W</ForeName><Initials>SW</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Resource Utilization, Hunan Agricultural University, Changsha, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tang</LastName><ForeName>M-L</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>Yantai Agricultural Science and Technology Institute, Yantai, 265500, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Z-Y</ForeName><Initials>ZY</Initials><AffiliationInfo><Affiliation>Institute of Plant Protection, Chinese Academy of Agricultural, Beijing 100193, China. The research was supported by the earmarked fund for Modern Agro-Industry Technology Research System (nycytx-30).</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30731973</ArticleId><ArticleId IdType="doi">10.1094/PDIS-11-10-0821</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/GrapevineDiseaseV1/Data/Main/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000679 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd -nk 000679 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= GrapevineDiseaseV1
|flux= Main
|étape= Corpus
|type= RBID
|clé= pubmed:30731973
|texte= First Report of Grapevine Trunk Disease Caused by Botryosphaeria obtusa in China.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Corpus/RBID.i -Sk "pubmed:30731973" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a GrapevineDiseaseV1
| This area was generated with Dilib version V0.6.37. |  |