Analyses of the temporal dynamics of fungal communities colonizing the healthy wood tissues of esca leaf-symptomatic and asymptomatic vines.
Identifieur interne : 000508 ( Main/Corpus ); précédent : 000507; suivant : 000509Analyses of the temporal dynamics of fungal communities colonizing the healthy wood tissues of esca leaf-symptomatic and asymptomatic vines.
Auteurs : Emilie Bruez ; Jessica Vallance ; Jonathan Gerbore ; Pascal Lecomte ; Jean-Pierre Da Costa ; Lucia Guerin-Dubrana ; Patrice ReySource :
- PloS one [ 1932-6203 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Fungal.
- classification : Fungi.
- genetics : Fungi.
- microbiology : Plant Diseases, Plant Leaves, Vitis, Wood.
- Biodiversity, Haplotypes, Microbiota.
Abstract
Esca, a Grapevine Trunk Disease (GTD), is of major concern for viticulture worldwide. Our study compares the fungal communities that inhabit the wood tissues of vines that expressed or not foliar esca-symptoms. The trunk and rootstock tissues were apparently healthy, whether the 10 year-old plants were symptomatic or not. The only difference was in the cordon, which contained white rot, a typical form of esca, in 79% of symptomatic plants. Observations over a period of one year using a fingerprint method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi, showed that shifts occurred in the fungal communities colonizing the healthy wood tissues. However, whatever the sampling time, spring, summer, autumn or winter, the fungi colonizing the healthy tissues of asymptomatic or symptomatic plants were not significantly different. Forty-eight genera were isolated, with species of Hypocreaceae and Botryosphaeriaceae being the most abundant species. Diverse fungal assemblages, made up of potentially plant-pathogenic and -protective fungi, colonized these non-necrotic tissues. Some fungi, possibly involved in GTD, inhabited the non-necrotic wood of young plants, but no increase in necrosis areas was observed over the one-year period.
DOI: 10.1371/journal.pone.0095928
PubMed: 24788412
PubMed Central: PMC4006835
Links to Exploration step
pubmed:24788412Le document en format XML
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INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France; BIOVITIS, Saint Etienne de Chomeil, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lecomte, Pascal" sort="Lecomte, Pascal" uniqKey="Lecomte P" first="Pascal" last="Lecomte">Pascal Lecomte</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Da Costa, Jean Pierre" sort="Da Costa, Jean Pierre" uniqKey="Da Costa J" first="Jean-Pierre" last="Da Costa">Jean-Pierre Da Costa</name><affiliation><nlm:affiliation>IMS, Université de Bordeaux, Bordeaux Sciences Agro, Talence, France.</nlm:affiliation></affiliation></author><author><name sortKey="Guerin Dubrana, Lucia" sort="Guerin Dubrana, Lucia" uniqKey="Guerin Dubrana L" first="Lucia" last="Guerin-Dubrana">Lucia Guerin-Dubrana</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Rey, Patrice" sort="Rey, Patrice" uniqKey="Rey P" first="Patrice" last="Rey">Patrice Rey</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:24788412</idno><idno type="pmid">24788412</idno><idno type="doi">10.1371/journal.pone.0095928</idno><idno type="pmc">PMC4006835</idno><idno type="wicri:Area/Main/Corpus">000508</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000508</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Analyses of the temporal dynamics of fungal communities colonizing the healthy wood tissues of esca leaf-symptomatic and asymptomatic vines.</title><author><name sortKey="Bruez, Emilie" sort="Bruez, Emilie" uniqKey="Bruez E" first="Emilie" last="Bruez">Emilie Bruez</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Vallance, Jessica" sort="Vallance, Jessica" uniqKey="Vallance J" first="Jessica" last="Vallance">Jessica Vallance</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Gerbore, Jonathan" sort="Gerbore, Jonathan" uniqKey="Gerbore J" first="Jonathan" last="Gerbore">Jonathan Gerbore</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France; BIOVITIS, Saint Etienne de Chomeil, France.</nlm:affiliation></affiliation></author><author><name sortKey="Lecomte, Pascal" sort="Lecomte, Pascal" uniqKey="Lecomte P" first="Pascal" last="Lecomte">Pascal Lecomte</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Da Costa, Jean Pierre" sort="Da Costa, Jean Pierre" uniqKey="Da Costa J" first="Jean-Pierre" last="Da Costa">Jean-Pierre Da Costa</name><affiliation><nlm:affiliation>IMS, Université de Bordeaux, Bordeaux Sciences Agro, Talence, France.</nlm:affiliation></affiliation></author><author><name sortKey="Guerin Dubrana, Lucia" sort="Guerin Dubrana, Lucia" uniqKey="Guerin Dubrana L" first="Lucia" last="Guerin-Dubrana">Lucia Guerin-Dubrana</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author><author><name sortKey="Rey, Patrice" sort="Rey, Patrice" uniqKey="Rey P" first="Patrice" last="Rey">Patrice Rey</name><affiliation><nlm:affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</nlm:affiliation></affiliation></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodiversity (MeSH)</term><term>DNA, Fungal (genetics)</term><term>Fungi (classification)</term><term>Fungi (genetics)</term><term>Haplotypes (MeSH)</term><term>Microbiota (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Vitis (microbiology)</term><term>Wood (microbiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Diseases</term><term>Plant Leaves</term><term>Vitis</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodiversity</term><term>Haplotypes</term><term>Microbiota</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Esca, a Grapevine Trunk Disease (GTD), is of major concern for viticulture worldwide. Our study compares the fungal communities that inhabit the wood tissues of vines that expressed or not foliar esca-symptoms. The trunk and rootstock tissues were apparently healthy, whether the 10 year-old plants were symptomatic or not. The only difference was in the cordon, which contained white rot, a typical form of esca, in 79% of symptomatic plants. Observations over a period of one year using a fingerprint method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi, showed that shifts occurred in the fungal communities colonizing the healthy wood tissues. However, whatever the sampling time, spring, summer, autumn or winter, the fungi colonizing the healthy tissues of asymptomatic or symptomatic plants were not significantly different. Forty-eight genera were isolated, with species of Hypocreaceae and Botryosphaeriaceae being the most abundant species. Diverse fungal assemblages, made up of potentially plant-pathogenic and -protective fungi, colonized these non-necrotic tissues. Some fungi, possibly involved in GTD, inhabited the non-necrotic wood of young plants, but no increase in necrosis areas was observed over the one-year period. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24788412</PMID><DateCompleted><Year>2014</Year><Month>12</Month><Day>18</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Electronic">1932-6203</ISSN><JournalIssue CitedMedium="Internet"><Volume>9</Volume><Issue>5</Issue><PubDate><Year>2014</Year></PubDate></JournalIssue><Title>PloS one</Title><ISOAbbreviation>PLoS One</ISOAbbreviation></Journal><ArticleTitle>Analyses of the temporal dynamics of fungal communities colonizing the healthy wood tissues of esca leaf-symptomatic and asymptomatic vines.</ArticleTitle><Pagination><MedlinePgn>e95928</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1371/journal.pone.0095928</ELocationID><Abstract><AbstractText>Esca, a Grapevine Trunk Disease (GTD), is of major concern for viticulture worldwide. Our study compares the fungal communities that inhabit the wood tissues of vines that expressed or not foliar esca-symptoms. The trunk and rootstock tissues were apparently healthy, whether the 10 year-old plants were symptomatic or not. The only difference was in the cordon, which contained white rot, a typical form of esca, in 79% of symptomatic plants. Observations over a period of one year using a fingerprint method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi, showed that shifts occurred in the fungal communities colonizing the healthy wood tissues. However, whatever the sampling time, spring, summer, autumn or winter, the fungi colonizing the healthy tissues of asymptomatic or symptomatic plants were not significantly different. Forty-eight genera were isolated, with species of Hypocreaceae and Botryosphaeriaceae being the most abundant species. Diverse fungal assemblages, made up of potentially plant-pathogenic and -protective fungi, colonized these non-necrotic tissues. Some fungi, possibly involved in GTD, inhabited the non-necrotic wood of young plants, but no increase in necrosis areas was observed over the one-year period. </AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bruez</LastName><ForeName>Emilie</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vallance</LastName><ForeName>Jessica</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerbore</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France; BIOVITIS, Saint Etienne de Chomeil, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lecomte</LastName><ForeName>Pascal</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Da Costa</LastName><ForeName>Jean-Pierre</ForeName><Initials>JP</Initials><AffiliationInfo><Affiliation>IMS, Université de Bordeaux, Bordeaux Sciences Agro, Talence, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guerin-Dubrana</LastName><ForeName>Lucia</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rey</LastName><ForeName>Patrice</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Université de Bordeaux, ISVV, UMR1065 Santé et Agroécologie du Vignoble (SAVE), Bordeaux Sciences Agro, Villenave d'Ornon, France; INRA, ISVV, UMR1065 SAVE, Villenave d'Ornon, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>05</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>PLoS One</MedlineTA><NlmUniqueID>101285081</NlmUniqueID><ISSNLinking>1932-6203</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D044822" MajorTopicYN="N">Biodiversity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006239" MajorTopicYN="N">Haplotypes</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064307" MajorTopicYN="Y">Microbiota</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027843" MajorTopicYN="N">Vitis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>10</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>12</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24788412</ArticleId><ArticleId IdType="doi">10.1371/journal.pone.0095928</ArticleId><ArticleId IdType="pii">PONE-D-13-43225</ArticleId><ArticleId IdType="pmc">PMC4006835</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>C R Biol. 2009 Sep;332(9):765-83</Citation><ArticleIdList><ArticleId IdType="pubmed">19748452</ArticleId></ArticleIdList></Reference><Reference><Citation>Phytopathology. 2007 Oct;97(10):1284-9</Citation><ArticleIdList><ArticleId IdType="pubmed">18943686</ArticleId></ArticleIdList></Reference><Reference><Citation>Microb Pathog. 2012 Apr;52(4):201-5</Citation><ArticleIdList><ArticleId IdType="pubmed">22261114</ArticleId></ArticleIdList></Reference><Reference><Citation>Ecology. 2007 Mar;88(3):541-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17503580</ArticleId></ArticleIdList></Reference><Reference><Citation>Appl Environ Microbiol. 2009 Jul;75(14):4790-800</Citation><ArticleIdList><ArticleId IdType="pubmed">19447961</ArticleId></ArticleIdList></Reference><Reference><Citation>Philos Trans R Soc Lond B Biol Sci. 2005 Feb 28;360(1454):243-54</Citation><ArticleIdList><ArticleId IdType="pubmed">15814343</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2010 Apr;186(2):281-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20409185</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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