DetoxFungiV1, Main, Exploration, bibRecord, 000509

Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene.

Identifieur interne : 000509 ( Main/Exploration ); précédent : 000508; suivant : 000510

Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene.

Auteurs : Orathai Pakdee [Thaïlande] ; Wisuwat Songnuan [Thaïlande] ; Nathinee Panvisavas [Thaïlande] ; Prayad Pokethitiyook [Thaïlande] ; Kittisak Yokthongwattana [Thaïlande] ; Metha Meetam [Thaïlande]

Source :

Planta [ 1432-2048 ] ; 2019.

RBID : pubmed:31037485

Descripteurs français

KwdFr :
- Alignement de séquences (MeSH), Bryopsida (génétique), Bryopsida (physiologie), Cadmium (toxicité), Chlorure de sodium (toxicité), Cuivre (toxicité), Isoformes de protéines (MeSH), Métallothionéine (génétique), Métallothionéine (métabolisme), Métaux lourds (toxicité), Peroxyde d'hydrogène (toxicité), Phylogenèse (MeSH), Protéines végétales (génétique), Protéines végétales (métabolisme), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (physiologie), Stress oxydatif (effets des médicaments et des substances chimiques), Stress physiologique (MeSH), Séquence d'acides aminés (MeSH), Techniques de knock-out de gènes (MeSH), Zinc (toxicité).
MESH :
- effets des médicaments et des substances chimiques : Stress oxydatif.
- génétique : Bryopsida, Métallothionéine, Protéines végétales, Saccharomyces cerevisiae.
- métabolisme : Métallothionéine, Protéines végétales.
- physiologie : Bryopsida, Saccharomyces cerevisiae.
- toxicité : Cadmium, Chlorure de sodium, Cuivre, Métaux lourds, Peroxyde d'hydrogène, Zinc.
- Alignement de séquences, Isoformes de protéines, Phylogenèse, Stress physiologique, Séquence d'acides aminés, Techniques de knock-out de gènes.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Bryopsida (genetics), Bryopsida (physiology), Cadmium (toxicity), Copper (toxicity), Gene Knockout Techniques (MeSH), Hydrogen Peroxide (toxicity), Metallothionein (genetics), Metallothionein (metabolism), Metals, Heavy (toxicity), Oxidative Stress (drug effects), Phylogeny (MeSH), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Isoforms (MeSH), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (physiology), Sequence Alignment (MeSH), Sodium Chloride (toxicity), Stress, Physiological (MeSH), Zinc (toxicity).
MESH :
- chemical , genetics : Metallothionein, Plant Proteins.
- chemical , metabolism : Metallothionein, Plant Proteins.
- chemical , toxicity : Cadmium, Copper, Hydrogen Peroxide, Metals, Heavy, Sodium Chloride, Zinc.
- drug effects : Oxidative Stress.
- genetics : Bryopsida, Saccharomyces cerevisiae.
- physiology : Bryopsida, Saccharomyces cerevisiae.
- Amino Acid Sequence, Gene Knockout Techniques, Phylogeny, Protein Isoforms, Sequence Alignment, Stress, Physiological.

Abstract

MAIN CONCLUSION

Physcomitrella patens contains four metallothionein-like genes. Three were shown to confer metal tolerance in yeast. Transcript profiling suggests their roles in senescence and reproductive development or cadmium and oxidative stress. Metallothioneins (MTs) have been suggested to play various roles including metal detoxification, nutrient remobilization, ROS scavenging, stress tolerance, and plant development. However, little is known about the forms and functions of MTs in bryophytes. The moss Physcomitrella patens genome was found to contain four MT-like genes. Amino acid sequence composition showed that the P. patens MTs (PpMTs) were clustered with Type 1 plant MTs, and could be further classified into two sub-types, herein referred to as sub-type 1: PpMT1.1a and PpMT1.1b and sub-type 2: PpMT1.2a and PpMT1.2b. Transcript abundance of PpMT1.1b and PpMT1.2b was upregulated in the gametophore compared to protonema, and all, except PpMT1.2a, were highly induced in senescing gametophytes. PpMT1.1a and PpMT1.1b transcripts were upregulated in protonema treated with cadmium and hydrogen peroxide. Unlike many higher plant MTs, the PpMT transcript abundance was not strongly induced in response to copper and zinc. These results suggest that PpMTs may play a role in protecting P. patens from cadmium and oxidative stress and may be involved in tissues senescence and reproductive development. The PpMTs, except PpMT1.2b, were also able to confer metal tolerance and accumulation when heterologously expressed in the ∆cup1 yeast. A P. patens mutant lacking PpMT1.2a through targeted gene disruption was generated. However, it did not show any alteration in growth phenotypes under senescence-induced conditions or hypersensitivity to cadmium, copper, zinc, H

DOI: 10.1007/s00425-019-03173-8
PubMed: 31037485

Affiliations:

Thaïlande

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Le document en format XML

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<author><name sortKey="Meetam, Metha" sort="Meetam, Metha" uniqKey="Meetam M" first="Metha" last="Meetam">Metha Meetam</name>
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<series><title level="j">Planta</title>
<idno type="eISSN">1432-2048</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term>
<term>Bryopsida (genetics)</term>
<term>Bryopsida (physiology)</term>
<term>Cadmium (toxicity)</term>
<term>Copper (toxicity)</term>
<term>Gene Knockout Techniques (MeSH)</term>
<term>Hydrogen Peroxide (toxicity)</term>
<term>Metallothionein (genetics)</term>
<term>Metallothionein (metabolism)</term>
<term>Metals, Heavy (toxicity)</term>
<term>Oxidative Stress (drug effects)</term>
<term>Phylogeny (MeSH)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Protein Isoforms (MeSH)</term>
<term>Saccharomyces cerevisiae (genetics)</term>
<term>Saccharomyces cerevisiae (physiology)</term>
<term>Sequence Alignment (MeSH)</term>
<term>Sodium Chloride (toxicity)</term>
<term>Stress, Physiological (MeSH)</term>
<term>Zinc (toxicity)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term>
<term>Bryopsida (génétique)</term>
<term>Bryopsida (physiologie)</term>
<term>Cadmium (toxicité)</term>
<term>Chlorure de sodium (toxicité)</term>
<term>Cuivre (toxicité)</term>
<term>Isoformes de protéines (MeSH)</term>
<term>Métallothionéine (génétique)</term>
<term>Métallothionéine (métabolisme)</term>
<term>Métaux lourds (toxicité)</term>
<term>Peroxyde d'hydrogène (toxicité)</term>
<term>Phylogenèse (MeSH)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Saccharomyces cerevisiae (génétique)</term>
<term>Saccharomyces cerevisiae (physiologie)</term>
<term>Stress oxydatif (effets des médicaments et des substances chimiques)</term>
<term>Stress physiologique (MeSH)</term>
<term>Séquence d'acides aminés (MeSH)</term>
<term>Techniques de knock-out de gènes (MeSH)</term>
<term>Zinc (toxicité)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Metallothionein</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Metallothionein</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cadmium</term>
<term>Copper</term>
<term>Hydrogen Peroxide</term>
<term>Metals, Heavy</term>
<term>Sodium Chloride</term>
<term>Zinc</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Oxidative Stress</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Stress oxydatif</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bryopsida</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bryopsida</term>
<term>Métallothionéine</term>
<term>Protéines végétales</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Métallothionéine</term>
<term>Protéines végétales</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Bryopsida</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Bryopsida</term>
<term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Cadmium</term>
<term>Chlorure de sodium</term>
<term>Cuivre</term>
<term>Métaux lourds</term>
<term>Peroxyde d'hydrogène</term>
<term>Zinc</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Gene Knockout Techniques</term>
<term>Phylogeny</term>
<term>Protein Isoforms</term>
<term>Sequence Alignment</term>
<term>Stress, Physiological</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term>
<term>Isoformes de protéines</term>
<term>Phylogenèse</term>
<term>Stress physiologique</term>
<term>Séquence d'acides aminés</term>
<term>Techniques de knock-out de gènes</term>
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<front><div type="abstract" xml:lang="en"><p><b>MAIN CONCLUSION</b>
</p>
<p>Physcomitrella patens contains four metallothionein-like genes. Three were shown to confer metal tolerance in yeast. Transcript profiling suggests their roles in senescence and reproductive development or cadmium and oxidative stress. Metallothioneins (MTs) have been suggested to play various roles including metal detoxification, nutrient remobilization, ROS scavenging, stress tolerance, and plant development. However, little is known about the forms and functions of MTs in bryophytes. The moss Physcomitrella patens genome was found to contain four MT-like genes. Amino acid sequence composition showed that the P. patens MTs (PpMTs) were clustered with Type 1 plant MTs, and could be further classified into two sub-types, herein referred to as sub-type 1: PpMT1.1a and PpMT1.1b and sub-type 2: PpMT1.2a and PpMT1.2b. Transcript abundance of PpMT1.1b and PpMT1.2b was upregulated in the gametophore compared to protonema, and all, except PpMT1.2a, were highly induced in senescing gametophytes. PpMT1.1a and PpMT1.1b transcripts were upregulated in protonema treated with cadmium and hydrogen peroxide. Unlike many higher plant MTs, the PpMT transcript abundance was not strongly induced in response to copper and zinc. These results suggest that PpMTs may play a role in protecting P. patens from cadmium and oxidative stress and may be involved in tissues senescence and reproductive development. The PpMTs, except PpMT1.2b, were also able to confer metal tolerance and accumulation when heterologously expressed in the ∆cup1 yeast. A P. patens mutant lacking PpMT1.2a through targeted gene disruption was generated. However, it did not show any alteration in growth phenotypes under senescence-induced conditions or hypersensitivity to cadmium, copper, zinc, H</p>
</div>
</front>
</TEI>
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<DateCompleted><Year>2019</Year>
<Month>08</Month>
<Day>05</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>02</Month>
<Day>25</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>250</Volume>
<Issue>2</Issue>
<PubDate><Year>2019</Year>
<Month>Aug</Month>
</PubDate>
</JournalIssue>
<Title>Planta</Title>
<ISOAbbreviation>Planta</ISOAbbreviation>
</Journal>
<ArticleTitle>Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene.</ArticleTitle>
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<ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-019-03173-8</ELocationID>
<Abstract><AbstractText Label="MAIN CONCLUSION" NlmCategory="UNASSIGNED">Physcomitrella patens contains four metallothionein-like genes. Three were shown to confer metal tolerance in yeast. Transcript profiling suggests their roles in senescence and reproductive development or cadmium and oxidative stress. Metallothioneins (MTs) have been suggested to play various roles including metal detoxification, nutrient remobilization, ROS scavenging, stress tolerance, and plant development. However, little is known about the forms and functions of MTs in bryophytes. The moss Physcomitrella patens genome was found to contain four MT-like genes. Amino acid sequence composition showed that the P. patens MTs (PpMTs) were clustered with Type 1 plant MTs, and could be further classified into two sub-types, herein referred to as sub-type 1: PpMT1.1a and PpMT1.1b and sub-type 2: PpMT1.2a and PpMT1.2b. Transcript abundance of PpMT1.1b and PpMT1.2b was upregulated in the gametophore compared to protonema, and all, except PpMT1.2a, were highly induced in senescing gametophytes. PpMT1.1a and PpMT1.1b transcripts were upregulated in protonema treated with cadmium and hydrogen peroxide. Unlike many higher plant MTs, the PpMT transcript abundance was not strongly induced in response to copper and zinc. These results suggest that PpMTs may play a role in protecting P. patens from cadmium and oxidative stress and may be involved in tissues senescence and reproductive development. The PpMTs, except PpMT1.2b, were also able to confer metal tolerance and accumulation when heterologously expressed in the ∆cup1 yeast. A P. patens mutant lacking PpMT1.2a through targeted gene disruption was generated. However, it did not show any alteration in growth phenotypes under senescence-induced conditions or hypersensitivity to cadmium, copper, zinc, H<sub>2</sub>
O<sub>2</sub>
, and NaCl stresses. Further characterization of additional P. patens mutants lacking single or multiple PpMTs may provide insight into the physiological roles of bryophytic MTs.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pakdee</LastName>
<ForeName>Orathai</ForeName>
<Initials>O</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-3920-5487</Identifier>
<AffiliationInfo><Affiliation>Department of Biology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Center of Excellence on Environmental Health and Toxicology, CHE, Ministry of Education, Bangkok, 10400, Thailand.</Affiliation>
</AffiliationInfo>
</Author>
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Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene.

Functional characterization of metallothionein-like genes from Physcomitrella patens: expression profiling, yeast heterologous expression, and disruption of PpMT1.2a gene.

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