Structural Basis for the Limited Response to Oxidative and Thiol-Conjugating Agents by Triosephosphate Isomerase From the Photosynthetic Bacteria Synechocystis.
Identifieur interne : 000214 ( Main/Exploration ); précédent : 000213; suivant : 000215Structural Basis for the Limited Response to Oxidative and Thiol-Conjugating Agents by Triosephosphate Isomerase From the Photosynthetic Bacteria Synechocystis.
Auteurs : Eduardo Castro-Torres [Mexique] ; Pedro Jimenez-Sandoval [Mexique] ; Eli Fernández-De Gortari [États-Unis] ; Margarita L Pez-Castillo [Mexique] ; Noe Baruch-Torres [Mexique] ; Marisol L Pez-Hidalgo [Mexique] ; Antolín Peralta-Castro [Mexique] ; Corina Díaz-Quezada [Mexique] ; Rogerio R. Sotelo-Mundo [Mexique] ; Claudia G. Benitez-Cardoza [Mexique] ; L Michel Espinoza-Fonseca [États-Unis] ; Adrian Ochoa-Leyva [Mexique] ; Luis G. Brieba [Mexique]Source :
- Frontiers in molecular biosciences [ 2296-889X ] ; 2018.
Abstract
In plants, the ancestral cyanobacterial triosephosphate isomerase (TPI) was replaced by a duplicated version of the cytosolic TPI. This isoform acquired a transit peptide for chloroplast localization and functions in the Calvin-Benson cycle. To gain insight into the reasons for this gene replacement in plants, we characterized the TPI from the photosynthetic bacteria Synechocystis (SyTPI). SyTPI presents typical TPI enzyme kinetics profiles and assembles as a homodimer composed of two subunits that arrange in a (β-α)8 fold. We found that oxidizing agents diamide (DA) and H2O2, as well as thiol-conjugating agents such as oxidized glutathione (GSSG) and methyl methanethiosulfonate (MMTS), do not inhibit the catalytic activity of SyTPI at concentrations required to inactivate plastidic and cytosolic TPIs from the plant model Arabidopsis thaliana (AtpdTPI and AtcTPI, respectively). The crystal structure of SyTPI revealed that each monomer contains three cysteines, C47, C127, and C176; however only the thiol group of C176 is solvent exposed. While AtcTPI and AtpdTPI are redox-regulated by chemical modifications of their accessible and reactive cysteines, we found that C176 of SyTPI is not sensitive to redox modification in vitro. Our data let us postulate that SyTPI was replaced by a eukaryotic TPI, because the latter contains redox-sensitive cysteines that may be subject to post-translational modifications required for modulating TPI's enzymatic activity.
DOI: 10.3389/fmolb.2018.00103
PubMed: 30538993
PubMed Central: PMC6277545
Affiliations:
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para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Sotelo Mundo, Rogerio R" sort="Sotelo Mundo, Rogerio R" uniqKey="Sotelo Mundo R" first="Rogerio R" last="Sotelo-Mundo">Rogerio R. 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Brieba</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30538993</idno><idno type="pmid">30538993</idno><idno type="doi">10.3389/fmolb.2018.00103</idno><idno type="pmc">PMC6277545</idno><idno type="wicri:Area/Main/Corpus">000186</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000186</idno><idno type="wicri:Area/Main/Curation">000186</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000186</idno><idno type="wicri:Area/Main/Exploration">000186</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Structural Basis for the Limited Response to Oxidative and Thiol-Conjugating Agents by Triosephosphate Isomerase From the Photosynthetic Bacteria <i>Synechocystis</i>.</title><author><name sortKey="Castro Torres, Eduardo" sort="Castro Torres, Eduardo" uniqKey="Castro Torres E" first="Eduardo" last="Castro-Torres">Eduardo Castro-Torres</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Jimenez Sandoval, Pedro" sort="Jimenez Sandoval, Pedro" uniqKey="Jimenez Sandoval P" first="Pedro" last="Jimenez-Sandoval">Pedro Jimenez-Sandoval</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Fernandez De Gortari, Eli" sort="Fernandez De Gortari, Eli" uniqKey="Fernandez De Gortari E" first="Eli" last="Fernández-De Gortari">Eli Fernández-De Gortari</name><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="L Pez Castillo, Margarita" sort="L Pez Castillo, Margarita" uniqKey="L Pez Castillo M" first="Margarita" last="L Pez-Castillo">Margarita L Pez-Castillo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Baruch Torres, Noe" sort="Baruch Torres, Noe" uniqKey="Baruch Torres N" first="Noe" last="Baruch-Torres">Noe Baruch-Torres</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="L Pez Hidalgo, Marisol" sort="L Pez Hidalgo, Marisol" uniqKey="L Pez Hidalgo M" first="Marisol" last="L Pez-Hidalgo">Marisol L Pez-Hidalgo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México</wicri:regionArea><wicri:noRegion>Ciudad de México</wicri:noRegion></affiliation></author><author><name sortKey="Peralta Castro, Antolin" sort="Peralta Castro, Antolin" uniqKey="Peralta Castro A" first="Antolín" last="Peralta-Castro">Antolín Peralta-Castro</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Diaz Quezada, Corina" sort="Diaz Quezada, Corina" uniqKey="Diaz Quezada C" first="Corina" last="Díaz-Quezada">Corina Díaz-Quezada</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author><author><name sortKey="Sotelo Mundo, Rogerio R" sort="Sotelo Mundo, Rogerio R" uniqKey="Sotelo Mundo R" first="Rogerio R" last="Sotelo-Mundo">Rogerio R. Sotelo-Mundo</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Estructura Biomolecular, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio de Estructura Biomolecular, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo</wicri:regionArea><wicri:noRegion>Hermosillo</wicri:noRegion></affiliation></author><author><name sortKey="Benitez Cardoza, Claudia G" sort="Benitez Cardoza, Claudia G" uniqKey="Benitez Cardoza C" first="Claudia G" last="Benitez-Cardoza">Claudia G. Benitez-Cardoza</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México</wicri:regionArea><wicri:noRegion>Ciudad de México</wicri:noRegion></affiliation></author><author><name sortKey="Espinoza Fonseca, L Michel" sort="Espinoza Fonseca, L Michel" uniqKey="Espinoza Fonseca L" first="L Michel" last="Espinoza-Fonseca">L Michel Espinoza-Fonseca</name><affiliation wicri:level="2"><nlm:affiliation>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author><author><name sortKey="Ochoa Leyva, Adrian" sort="Ochoa Leyva, Adrian" uniqKey="Ochoa Leyva A" first="Adrian" last="Ochoa-Leyva">Adrian Ochoa-Leyva</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca</wicri:regionArea><wicri:noRegion>Cuernavaca</wicri:noRegion></affiliation></author><author><name sortKey="Brieba, Luis G" sort="Brieba, Luis G" uniqKey="Brieba L" first="Luis G" last="Brieba">Luis G. Brieba</name><affiliation wicri:level="1"><nlm:affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</nlm:affiliation><country xml:lang="fr">Mexique</country><wicri:regionArea>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato</wicri:regionArea><wicri:noRegion>Guanajuato</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in molecular biosciences</title><idno type="ISSN">2296-889X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In plants, the ancestral cyanobacterial triosephosphate isomerase (TPI) was replaced by a duplicated version of the cytosolic TPI. This isoform acquired a transit peptide for chloroplast localization and functions in the Calvin-Benson cycle. To gain insight into the reasons for this gene replacement in plants, we characterized the TPI from the photosynthetic bacteria <i>Synechocystis</i> (SyTPI). SyTPI presents typical TPI enzyme kinetics profiles and assembles as a homodimer composed of two subunits that arrange in a (β-α)<sub>8</sub> fold. We found that oxidizing agents diamide (DA) and H<sub>2</sub>O<sub>2</sub>, as well as thiol-conjugating agents such as oxidized glutathione (GSSG) and methyl methanethiosulfonate (MMTS), do not inhibit the catalytic activity of SyTPI at concentrations required to inactivate plastidic and cytosolic TPIs from the plant model <i>Arabidopsis thaliana</i> (AtpdTPI and AtcTPI, respectively). The crystal structure of SyTPI revealed that each monomer contains three cysteines, C47, C127, and C176; however only the thiol group of C176 is solvent exposed. While AtcTPI and AtpdTPI are redox-regulated by chemical modifications of their accessible and reactive cysteines, we found that C176 of SyTPI is not sensitive to redox modification <i>in vitro</i>. Our data let us postulate that SyTPI was replaced by a eukaryotic TPI, because the latter contains redox-sensitive cysteines that may be subject to post-translational modifications required for modulating TPI's enzymatic activity.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30538993</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">2296-889X</ISSN><JournalIssue CitedMedium="Print"><Volume>5</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in molecular biosciences</Title><ISOAbbreviation>Front Mol Biosci</ISOAbbreviation></Journal><ArticleTitle>Structural Basis for the Limited Response to Oxidative and Thiol-Conjugating Agents by Triosephosphate Isomerase From the Photosynthetic Bacteria <i>Synechocystis</i>.</ArticleTitle><Pagination><MedlinePgn>103</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmolb.2018.00103</ELocationID><Abstract><AbstractText>In plants, the ancestral cyanobacterial triosephosphate isomerase (TPI) was replaced by a duplicated version of the cytosolic TPI. This isoform acquired a transit peptide for chloroplast localization and functions in the Calvin-Benson cycle. To gain insight into the reasons for this gene replacement in plants, we characterized the TPI from the photosynthetic bacteria <i>Synechocystis</i> (SyTPI). SyTPI presents typical TPI enzyme kinetics profiles and assembles as a homodimer composed of two subunits that arrange in a (β-α)<sub>8</sub> fold. We found that oxidizing agents diamide (DA) and H<sub>2</sub>O<sub>2</sub>, as well as thiol-conjugating agents such as oxidized glutathione (GSSG) and methyl methanethiosulfonate (MMTS), do not inhibit the catalytic activity of SyTPI at concentrations required to inactivate plastidic and cytosolic TPIs from the plant model <i>Arabidopsis thaliana</i> (AtpdTPI and AtcTPI, respectively). The crystal structure of SyTPI revealed that each monomer contains three cysteines, C47, C127, and C176; however only the thiol group of C176 is solvent exposed. While AtcTPI and AtpdTPI are redox-regulated by chemical modifications of their accessible and reactive cysteines, we found that C176 of SyTPI is not sensitive to redox modification <i>in vitro</i>. Our data let us postulate that SyTPI was replaced by a eukaryotic TPI, because the latter contains redox-sensitive cysteines that may be subject to post-translational modifications required for modulating TPI's enzymatic activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Castro-Torres</LastName><ForeName>Eduardo</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jimenez-Sandoval</LastName><ForeName>Pedro</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fernández-de Gortari</LastName><ForeName>Eli</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Castillo</LastName><ForeName>Margarita</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Baruch-Torres</LastName><ForeName>Noe</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>López-Hidalgo</LastName><ForeName>Marisol</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peralta-Castro</LastName><ForeName>Antolín</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Díaz-Quezada</LastName><ForeName>Corina</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sotelo-Mundo</LastName><ForeName>Rogerio R</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Laboratorio de Estructura Biomolecular, Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Benitez-Cardoza</LastName><ForeName>Claudia G</ForeName><Initials>CG</Initials><AffiliationInfo><Affiliation>Laboratorio de Investigación Bioquímica, Programa Institucional en Biomedicina Molecular ENMyH-IPN, Ciudad de México, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Espinoza-Fonseca</LastName><ForeName>L Michel</ForeName><Initials>LM</Initials><AffiliationInfo><Affiliation>Division of Cardiovascular Medicine, Department of Internal Medicine, Center for Arrhythmia Research, University of Michigan, Ann Arbor, MI, United States.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ochoa-Leyva</LastName><ForeName>Adrian</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Departamento de Microbiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brieba</LastName><ForeName>Luis G</ForeName><Initials>LG</Initials><AffiliationInfo><Affiliation>Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Guanajuato, Mexico.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>11</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Mol Biosci</MedlineTA><NlmUniqueID>101653173</NlmUniqueID><ISSNLinking>2296-889X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">X-ray structure</Keyword><Keyword MajorTopicYN="N">oxidative damage</Keyword><Keyword MajorTopicYN="N">protein evolution</Keyword><Keyword MajorTopicYN="N">thiol-based redox regulation</Keyword><Keyword MajorTopicYN="N">triosephosphate isomerase</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>04</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>11</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>12</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>12</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">28378917</ArticleId></ArticleIdList></Reference><Reference><Citation>Eukaryot Cell. 2013 Jun;12(6):776-93</Citation><ArticleIdList><ArticleId IdType="pubmed">23543671</ArticleId></ArticleIdList></Reference><Reference><Citation>Biophys J. 2017 Dec 5;113(11):2354-2363</Citation><ArticleIdList><ArticleId IdType="pubmed">29211989</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1988 Feb 23;27(4):1158-67</Citation><ArticleIdList><ArticleId IdType="pubmed">3365378</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant. 2014 Jan;7(1):101-20</Citation><ArticleIdList><ArticleId IdType="pubmed">24157611</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2015 Nov 30;10(11):e0141747</Citation><ArticleIdList><ArticleId IdType="pubmed">26618356</ArticleId></ArticleIdList></Reference><Reference><Citation>Genome Inform. 2005;16(2):56-68</Citation><ArticleIdList><ArticleId IdType="pubmed">16901089</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem J. 2016 Dec 1;473(23):4413-4426</Citation><ArticleIdList><ArticleId IdType="pubmed">27647935</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1999 Apr 27;96(9):4862-7</Citation><ArticleIdList><ArticleId IdType="pubmed">10220384</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioessays. 2008 Jun;30(6):556-66</Citation><ArticleIdList><ArticleId IdType="pubmed">18478535</ArticleId></ArticleIdList></Reference><Reference><Citation>J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674</Citation><ArticleIdList><ArticleId IdType="pubmed">19461840</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS Lett. 1999 Sep 10;458(1):69-71</Citation><ArticleIdList><ArticleId IdType="pubmed">10518936</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1990 Jul 17;29(28):6609-18</Citation><ArticleIdList><ArticleId IdType="pubmed">2204417</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein J. 2010 Jan;29(1):1-10</Citation><ArticleIdList><ArticleId IdType="pubmed">19924403</ArticleId></ArticleIdList></Reference><Reference><Citation>Anal Biochem. 2004 Sep 1;332(1):153-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15301960</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2012 Apr;185-186:86-96</Citation><ArticleIdList><ArticleId IdType="pubmed">22325869</ArticleId></ArticleIdList></Reference><Reference><Citation>Free Radic Biol Med. 2015 Mar;80:148-57</Citation><ArticleIdList><ArticleId IdType="pubmed">25433365</ArticleId></ArticleIdList></Reference><Reference><Citation>Cell Mol Life Sci. 2010 Dec;67(23):3961-82</Citation><ArticleIdList><ArticleId IdType="pubmed">20694739</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteome Res. 2015 Jan 2;14(1):59-71</Citation><ArticleIdList><ArticleId IdType="pubmed">25208982</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2003 Jul;44(7):655-60</Citation><ArticleIdList><ArticleId IdType="pubmed">12881492</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2002 Jul 26;295(4):958-63</Citation><ArticleIdList><ArticleId IdType="pubmed">12127988</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteins. 2014 Jan;82(1):22-33</Citation><ArticleIdList><ArticleId IdType="pubmed">23733417</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2004 Aug 27;341(5):1355-65</Citation><ArticleIdList><ArticleId IdType="pubmed">15321726</ArticleId></ArticleIdList></Reference><Reference><Citation>Proteomics. 2010 Mar;10(5):987-1001</Citation><ArticleIdList><ArticleId IdType="pubmed">20049866</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1992 Apr 20;224(4):1115-26</Citation><ArticleIdList><ArticleId IdType="pubmed">1569570</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2012;13(8):10010-21</Citation><ArticleIdList><ArticleId IdType="pubmed">22949845</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 1976 Dec 14;15(25):5627-31</Citation><ArticleIdList><ArticleId IdType="pubmed">999838</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Sci. 1994 May;3(5):810-21</Citation><ArticleIdList><ArticleId IdType="pubmed">8061610</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS J. 2007 Jan;274(1):212-26</Citation><ArticleIdList><ArticleId IdType="pubmed">17140414</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2001 Mar 2;306(4):745-57</Citation><ArticleIdList><ArticleId IdType="pubmed">11243785</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2006 Feb 28;45(8):2556-60</Citation><ArticleIdList><ArticleId IdType="pubmed">16489748</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Nov;169(3):1469-87</Citation><ArticleIdList><ArticleId IdType="pubmed">26338952</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Protoc Protein Sci. 2015 Feb 02;79:28.9.1-14</Citation><ArticleIdList><ArticleId IdType="pubmed">25640896</ArticleId></ArticleIdList></Reference><Reference><Citation>Bioorg Med Chem Lett. 2006 Dec 15;16(24):6288-92</Citation><ArticleIdList><ArticleId IdType="pubmed">16997551</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2004 Mar 19;32(5):1792-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15034147</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2010 Jul;38(Web Server issue):W407-11</Citation><ArticleIdList><ArticleId IdType="pubmed">20525787</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Chem. 2005 Dec;26(16):1781-802</Citation><ArticleIdList><ArticleId IdType="pubmed">16222654</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2008 Aug 1;283(31):21571-8</Citation><ArticleIdList><ArticleId IdType="pubmed">18534986</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Evol Biol. 2008 Apr 23;8:117</Citation><ArticleIdList><ArticleId IdType="pubmed">18433492</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2016 Dec 22;7:1942</Citation><ArticleIdList><ArticleId IdType="pubmed">28066493</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2016 Dec 06;7:1817</Citation><ArticleIdList><ArticleId IdType="pubmed">27999583</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Biol Evol. 2016 Jul;33(7):1870-4</Citation><ArticleIdList><ArticleId IdType="pubmed">27004904</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2016 Dec;1864(12):1696-1706</Citation><ArticleIdList><ArticleId IdType="pubmed">27614148</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1959 May;82(1):70-7</Citation><ArticleIdList><ArticleId IdType="pubmed">13650640</ArticleId></ArticleIdList></Reference><Reference><Citation>J Am Chem Soc. 2015 Dec 9;137(48):15185-97</Citation><ArticleIdList><ArticleId IdType="pubmed">26570983</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2016 May 10;113(19):5441-6</Citation><ArticleIdList><ArticleId IdType="pubmed">27114545</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Biochem Biophys. 1971 Sep;146(1):312-20</Citation><ArticleIdList><ArticleId IdType="pubmed">5169140</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Plant Biol. 2008;59:491-517</Citation><ArticleIdList><ArticleId IdType="pubmed">18315522</ArticleId></ArticleIdList></Reference><Reference><Citation>Mass Spectrom Rev. 2017 Nov;36(6):677-692</Citation><ArticleIdList><ArticleId IdType="pubmed">26763661</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Chem. 2010 Jan 30;31(2):455-61</Citation><ArticleIdList><ArticleId IdType="pubmed">19499576</ArticleId></ArticleIdList></Reference><Reference><Citation>Redox Biol. 2018 Jul;17:35-46</Citation><ArticleIdList><ArticleId IdType="pubmed">29673699</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochim Biophys Acta. 2015 Aug;1850(8):1479-96</Citation><ArticleIdList><ArticleId IdType="pubmed">25676896</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Nov 12;4:450</Citation><ArticleIdList><ArticleId IdType="pubmed">24282406</ArticleId></ArticleIdList></Reference><Reference><Citation>Antioxid Redox Signal. 2012 Mar 15;16(6):567-86</Citation><ArticleIdList><ArticleId IdType="pubmed">22053845</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2015 Apr 10;427(7):1549-63</Citation><ArticleIdList><ArticleId IdType="pubmed">25698115</ArticleId></ArticleIdList></Reference><Reference><Citation>FEBS J. 2011 Jun;278(11):1932-43</Citation><ArticleIdList><ArticleId IdType="pubmed">21447068</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Jun 16;106 Suppl 1:9995-10000</Citation><ArticleIdList><ArticleId IdType="pubmed">19528653</ArticleId></ArticleIdList></Reference><Reference><Citation>DNA Res. 1996 Jun 30;3(3):109-36</Citation><ArticleIdList><ArticleId IdType="pubmed">8905231</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 1973 Oct 10;248(19):6583-91</Citation><ArticleIdList><ArticleId IdType="pubmed">4583259</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Enzymol. 1995;251:123-33</Citation><ArticleIdList><ArticleId IdType="pubmed">7651192</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Med Chem. 2010 Dec;45(12):5767-72</Citation><ArticleIdList><ArticleId IdType="pubmed">20889239</ArticleId></ArticleIdList></Reference><Reference><Citation>Protein Sci. 2014 Feb;23(2):213-28</Citation><ArticleIdList><ArticleId IdType="pubmed">24318986</ArticleId></ArticleIdList></Reference><Reference><Citation>J Proteomics. 2009 May 2;72(4):677-89</Citation><ArticleIdList><ArticleId IdType="pubmed">19367685</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 Nov 25;4:470</Citation><ArticleIdList><ArticleId IdType="pubmed">24324475</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2004 Mar 23;43(11):3255-63</Citation><ArticleIdList><ArticleId IdType="pubmed">15023076</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2011 Oct 14;413(1):195-208</Citation><ArticleIdList><ArticleId 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Sotelo-Mundo</name></country><country name="États-Unis"><region name="Michigan"><name sortKey="Fernandez De Gortari, Eli" sort="Fernandez De Gortari, Eli" uniqKey="Fernandez De Gortari E" first="Eli" last="Fernández-De Gortari">Eli Fernández-De Gortari</name></region><name sortKey="Espinoza Fonseca, L Michel" sort="Espinoza Fonseca, L Michel" uniqKey="Espinoza Fonseca L" first="L Michel" last="Espinoza-Fonseca">L Michel Espinoza-Fonseca</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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