Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom.
Identifieur interne : 000162 ( Main/Exploration ); précédent : 000161; suivant : 000163Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom.
Auteurs : Avia Mizrachi [Israël] ; Shiri Graff Van Creveld [Israël] ; Orr H. Shapiro [Israël] ; Shilo Rosenwasser [Israël] ; Assaf Vardi [Israël]Source :
- eLife [ 2050-084X ] ; 2019.
Descripteurs français
- KwdFr :
- Chloroplastes (métabolisme), Diatomées (effets des médicaments et des substances chimiques), Diatomées (effets des radiations), Diatomées (physiologie), Lumière (MeSH), Oxydoréduction (MeSH), Peroxyde d'hydrogène (toxicité), Stress physiologique (MeSH), Survie cellulaire (effets des médicaments et des substances chimiques), Survie cellulaire (effets des radiations).
- MESH :
- effets des médicaments et des substances chimiques : Diatomées, Survie cellulaire.
- effets des radiations : Diatomées, Survie cellulaire.
- métabolisme : Chloroplastes.
- physiologie : Diatomées.
- toxicité : Peroxyde d'hydrogène.
- Lumière, Oxydoréduction, Stress physiologique.
English descriptors
- KwdEn :
- MESH :
- chemical , toxicity : Hydrogen Peroxide.
- drug effects : Cell Survival, Diatoms.
- metabolism : Chloroplasts.
- physiology : Diatoms.
- radiation effects : Cell Survival, Diatoms.
- Light, Oxidation-Reduction, Stress, Physiological.
Abstract
Diatoms are photosynthetic microorganisms of great ecological and biogeochemical importance, forming vast blooms in aquatic ecosystems. However, we are still lacking fundamental understanding of how individual cells sense and respond to diverse stress conditions, and what acclimation strategies are employed during bloom dynamics. We investigated cellular responses to environmental stress at the single-cell level using the redox sensor roGFP targeted to various organelles in the diatom Phaeodactylum tricornutum. We detected cell-to-cell variability using flow cytometry cell sorting and a microfluidics system for live imaging of oxidation dynamics. Chloroplast-targeted roGFP exhibited a light-dependent, bi-stable oxidation pattern in response to H2O2 and high light, revealing distinct subpopulations of sensitive oxidized cells and resilient reduced cells. Early oxidation in the chloroplast preceded commitment to cell death, and can be used for sensing stress cues and regulating cell fate. We propose that light-dependent metabolic heterogeneity regulates diatoms' sensitivity to environmental stressors in the ocean.
DOI: 10.7554/eLife.47732
PubMed: 31232691
PubMed Central: PMC6682412
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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However, we are still lacking fundamental understanding of how individual cells sense and respond to diverse stress conditions, and what acclimation strategies are employed during bloom dynamics. We investigated cellular responses to environmental stress at the single-cell level using the redox sensor roGFP targeted to various organelles in the diatom <i>Phaeodactylum tricornutum</i>. We detected cell-to-cell variability using flow cytometry cell sorting and a microfluidics system for live imaging of oxidation dynamics. Chloroplast-targeted roGFP exhibited a light-dependent, bi-stable oxidation pattern in response to H<sub>2</sub>O<sub>2</sub> and high light, revealing distinct subpopulations of sensitive oxidized cells and resilient reduced cells. Early oxidation in the chloroplast preceded commitment to cell death, and can be used for sensing stress cues and regulating cell fate. We propose that light-dependent metabolic heterogeneity regulates diatoms' sensitivity to environmental stressors in the ocean.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31232691</PMID><DateCompleted><Year>2020</Year><Month>02</Month><Day>25</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>09</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2050-084X</ISSN><JournalIssue CitedMedium="Internet"><Volume>8</Volume><PubDate><Year>2019</Year><Month>06</Month><Day>24</Day></PubDate></JournalIssue><Title>eLife</Title><ISOAbbreviation>Elife</ISOAbbreviation></Journal><ArticleTitle>Light-dependent single-cell heterogeneity in the chloroplast redox state regulates cell fate in a marine diatom.</ArticleTitle><ELocationID EIdType="doi" ValidYN="Y">10.7554/eLife.47732</ELocationID><ELocationID EIdType="pii" ValidYN="Y">e47732</ELocationID><Abstract><AbstractText>Diatoms are photosynthetic microorganisms of great ecological and biogeochemical importance, forming vast blooms in aquatic ecosystems. However, we are still lacking fundamental understanding of how individual cells sense and respond to diverse stress conditions, and what acclimation strategies are employed during bloom dynamics. We investigated cellular responses to environmental stress at the single-cell level using the redox sensor roGFP targeted to various organelles in the diatom <i>Phaeodactylum tricornutum</i>. We detected cell-to-cell variability using flow cytometry cell sorting and a microfluidics system for live imaging of oxidation dynamics. Chloroplast-targeted roGFP exhibited a light-dependent, bi-stable oxidation pattern in response to H<sub>2</sub>O<sub>2</sub> and high light, revealing distinct subpopulations of sensitive oxidized cells and resilient reduced cells. Early oxidation in the chloroplast preceded commitment to cell death, and can be used for sensing stress cues and regulating cell fate. We propose that light-dependent metabolic heterogeneity regulates diatoms' sensitivity to environmental stressors in the ocean.</AbstractText><CopyrightInformation>© 2019, Mizrachi et al.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mizrachi</LastName><ForeName>Avia</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0001-7724-9275</Identifier><AffiliationInfo><Affiliation>Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Graff van Creveld</LastName><ForeName>Shiri</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0002-3445-3046</Identifier><AffiliationInfo><Affiliation>Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shapiro</LastName><ForeName>Orr H</ForeName><Initials>OH</Initials><Identifier Source="ORCID">0000-0002-3222-9809</Identifier><AffiliationInfo><Affiliation>Department of Food Quality and Safety, Institute of Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rosenwasser</LastName><ForeName>Shilo</ForeName><Initials>S</Initials><Identifier Source="ORCID">0000-0001-8565-9979</Identifier><AffiliationInfo><Affiliation>Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vardi</LastName><ForeName>Assaf</ForeName><Initials>A</Initials><Identifier Source="ORCID">0000-0002-7079-0234</Identifier><AffiliationInfo><Affiliation>Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>712233</GrantID><Agency>Israel Science Foundation</Agency><Country>International</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>06</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Elife</MedlineTA><NlmUniqueID>101579614</NlmUniqueID><ISSNLinking>2050-084X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002470" MajorTopicYN="N">Cell Survival</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000528" MajorTopicYN="N">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017377" MajorTopicYN="N">Diatoms</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName><QualifierName UI="Q000528" MajorTopicYN="Y">radiation effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000633" MajorTopicYN="N">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008027" MajorTopicYN="Y">Light</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010084" MajorTopicYN="N">Oxidation-Reduction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">ROS</Keyword><Keyword MajorTopicYN="Y">cell fate</Keyword><Keyword MajorTopicYN="Y">chloroplast</Keyword><Keyword MajorTopicYN="Y">diatom</Keyword><Keyword MajorTopicYN="Y">ecology</Keyword><Keyword MajorTopicYN="Y">infectious disease</Keyword><Keyword MajorTopicYN="Y">microbiology</Keyword><Keyword MajorTopicYN="Y">phenotypic variability</Keyword><Keyword MajorTopicYN="Y">single-cell</Keyword></KeywordList><CoiStatement>AM, SG, OS, SR, AV No competing interests declared</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>06</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId 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