Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts.
Identifieur interne : 000156 ( Main/Corpus ); précédent : 000155; suivant : 000157Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts.
Auteurs : Markéta Hájková ; Marie Kummerová ; Št Pán Zezulka ; Petr Babula ; Peter VácziSource :
- Chemosphere [ 1879-1298 ] ; 2019.
English descriptors
- KwdEn :
- Araceae (drug effects), Araceae (growth & development), Araceae (ultrastructure), Chloroplasts (drug effects), Chloroplasts (metabolism), Diclofenac (pharmacology), Diclofenac (toxicity), Electron Transport (drug effects), Lipid Peroxidation (drug effects), Oxidative Stress (drug effects), Photosynthesis (drug effects), Photosystem I Protein Complex (MeSH), Photosystem II Protein Complex (drug effects), Photosystem II Protein Complex (metabolism).
- MESH :
- chemical , drug effects : Photosystem II Protein Complex.
- chemical , metabolism : Photosystem II Protein Complex.
- chemical , pharmacology : Diclofenac.
- drug effects : Araceae, Chloroplasts, Electron Transport, Lipid Peroxidation, Oxidative Stress, Photosynthesis.
- growth & development : Araceae.
- metabolism : Chloroplasts.
- chemical , toxicity : Diclofenac.
- ultrastructure : Araceae.
- chemical : Photosystem I Protein Complex.
Abstract
Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 μM DCF. Decreases in the potential and effective quantum yields of photosystem II (FV/FM by 21%, ΦII by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 μM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 μM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 μM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 μM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 μM DCF, and lipid peroxidation increased even at 1 μM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.
DOI: 10.1016/j.chemosphere.2019.02.197
PubMed: 30986895
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Electronic address: kumerova@sci.muni.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Zezulka, St Pan" sort="Zezulka, St Pan" uniqKey="Zezulka S" first="Št Pán" last="Zezulka">Št Pán Zezulka</name><affiliation><nlm:affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: zezulka@sci.muni.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Babula, Petr" sort="Babula, Petr" uniqKey="Babula P" first="Petr" last="Babula">Petr Babula</name><affiliation><nlm:affiliation>Department of Physiology, Faculty of Medicine, Masaryk University Brno, Kamenice 753/5, 625 00 Brno, Czech Republic. 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Electronic address: hajkova.marketa@email.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Kummerova, Marie" sort="Kummerova, Marie" uniqKey="Kummerova M" first="Marie" last="Kummerová">Marie Kummerová</name><affiliation><nlm:affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: kumerova@sci.muni.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Zezulka, St Pan" sort="Zezulka, St Pan" uniqKey="Zezulka S" first="Št Pán" last="Zezulka">Št Pán Zezulka</name><affiliation><nlm:affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: zezulka@sci.muni.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Babula, Petr" sort="Babula, Petr" uniqKey="Babula P" first="Petr" last="Babula">Petr Babula</name><affiliation><nlm:affiliation>Department of Physiology, Faculty of Medicine, Masaryk University Brno, Kamenice 753/5, 625 00 Brno, Czech Republic. Electronic address: babula@med.muni.cz.</nlm:affiliation></affiliation></author><author><name sortKey="Vaczi, Peter" sort="Vaczi, Peter" uniqKey="Vaczi P" first="Peter" last="Váczi">Peter Váczi</name><affiliation><nlm:affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: vaczi@sci.muni.cz.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Araceae (drug effects)</term><term>Araceae (growth & development)</term><term>Araceae (ultrastructure)</term><term>Chloroplasts (drug effects)</term><term>Chloroplasts (metabolism)</term><term>Diclofenac (pharmacology)</term><term>Diclofenac (toxicity)</term><term>Electron Transport (drug effects)</term><term>Lipid Peroxidation (drug effects)</term><term>Oxidative Stress (drug effects)</term><term>Photosynthesis (drug effects)</term><term>Photosystem I Protein Complex (MeSH)</term><term>Photosystem II Protein Complex (drug effects)</term><term>Photosystem II Protein Complex (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Photosystem II Protein Complex</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Photosystem II Protein Complex</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Diclofenac</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Araceae</term><term>Chloroplasts</term><term>Electron Transport</term><term>Lipid Peroxidation</term><term>Oxidative Stress</term><term>Photosynthesis</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Araceae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chloroplasts</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Diclofenac</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Araceae</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Photosystem I Protein Complex</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 μM DCF. Decreases in the potential and effective quantum yields of photosystem II (F<sub>V</sub>/F<sub>M</sub> by 21%, Φ<sub>II</sub> by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 μM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 μM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 μM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 μM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 μM DCF, and lipid peroxidation increased even at 1 μM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">30986895</PMID><DateCompleted><Year>2019</Year><Month>06</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>06</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1298</ISSN><JournalIssue CitedMedium="Internet"><Volume>224</Volume><PubDate><Year>2019</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>Diclofenac as an environmental threat: Impact on the photosynthetic processes of Lemna minor chloroplasts.</ArticleTitle><Pagination><MedlinePgn>892-899</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0045-6535(19)30427-8</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2019.02.197</ELocationID><Abstract><AbstractText>Mechanisms of pharmaceuticals action on biochemical and physiological processes in plants that determine plant growth and development are still mostly unknown. This study deals with the effects of non-steroidal anti-inflammatory drug diclofenac (DCF) on photosynthesis as an essential anabolic process. Changes in primary and secondary photosynthetic processes were assessed in chloroplasts isolated from Lemna minor exposed to 1, 10, 100, and 1000 μM DCF. Decreases in the potential and effective quantum yields of photosystem II (F<sub>V</sub>/F<sub>M</sub> by 21%, Φ<sub>II</sub> by 44% compared to control), changes in non-photochemical fluorescence quenching (NPQ), and a substantial drop in Hill reaction activity (by 73%), especially under 1000 μM DCF, were found. Limitation of electron transport through photosystem II was confirmed by increased fluorescence signals in steps J and I (by 50% and 23%, respectively, under 1000 μM DCF) in OJIP fluorescence transient. Photosystem I exhibited changes only in the redox state of P700 reaction centres (decrease in Pm by 10%, increase in reduced P700 by 5% under 1000 μM DCF). Similarly, RuBisCO activity was only lowered by 30% under 1000 μM DCF. In contrast, a significant increase in reactive oxygen and nitrogen species (by 116% and 157%, respectively) was observed under 10 μM DCF, and lipid peroxidation increased even at 1 μM DCF (by nearly seven times compared to the control). Results demonstrate the ability of environmentally relevant DCF concentrations to induce oxidative stress in isolated duckweed chloroplasts; however, photosynthetic processes were affected considerably only by the highest DCF treatments.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hájková</LastName><ForeName>Markéta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: hajkova.marketa@email.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kummerová</LastName><ForeName>Marie</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: kumerova@sci.muni.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zezulka</LastName><ForeName>Štěpán</ForeName><Initials>Š</Initials><AffiliationInfo><Affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: zezulka@sci.muni.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babula</LastName><ForeName>Petr</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Physiology, Faculty of Medicine, Masaryk University Brno, Kamenice 753/5, 625 00 Brno, Czech Republic. Electronic address: babula@med.muni.cz.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Váczi</LastName><ForeName>Peter</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Plant Physiology and Anatomy, Institute of Experimental Biology, Faculty of Science, Masaryk University Brno, Kotlářská 2, 611 37 Brno, Czech Republic. Electronic address: vaczi@sci.muni.cz.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>03</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045331">Photosystem I Protein Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D045332">Photosystem II Protein Complex</NameOfSubstance></Chemical><Chemical><RegistryNumber>144O8QL0L1</RegistryNumber><NameOfSubstance UI="D004008">Diclofenac</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D029064" MajorTopicYN="N">Araceae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002736" MajorTopicYN="N">Chloroplasts</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004008" MajorTopicYN="N">Diclofenac</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName><QualifierName UI="Q000633" MajorTopicYN="Y">toxicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004579" MajorTopicYN="N">Electron Transport</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015227" MajorTopicYN="N">Lipid Peroxidation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010788" MajorTopicYN="N">Photosynthesis</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045331" MajorTopicYN="N">Photosystem I Protein Complex</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045332" MajorTopicYN="N">Photosystem II Protein Complex</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Diclofenac</Keyword><Keyword MajorTopicYN="N">Duckweed chloroplasts</Keyword><Keyword MajorTopicYN="N">Oxidative stress</Keyword><Keyword MajorTopicYN="N">Photosystems II and I</Keyword><Keyword MajorTopicYN="N">RuBisCO</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>02</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>02</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>4</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>6</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30986895</ArticleId><ArticleId IdType="pii">S0045-6535(19)30427-8</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2019.02.197</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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