Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism
Identifieur interne : 000008 ( Pmc/Curation ); précédent : 000007; suivant : 000009Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism
Auteurs : João Paulo Coutinho ; Ana Carvalho ; José Lima-BritoSource :
- AoB Plants [ 2041-2851 ] ; 2014.
Abstract
In 30 Fagaceae individuals from the
Url:
DOI: 10.1093/aobpla/plu079
PubMed: 25429047
PubMed Central: 4294445
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism</title><author><name sortKey="Coutinho, Joao Paulo" sort="Coutinho, Joao Paulo" uniqKey="Coutinho J" first="João Paulo" last="Coutinho">João Paulo Coutinho</name></author><author><name sortKey="Carvalho, Ana" sort="Carvalho, Ana" uniqKey="Carvalho A" first="Ana" last="Carvalho">Ana Carvalho</name></author><author><name sortKey="Lima Brito, Jose" sort="Lima Brito, Jose" uniqKey="Lima Brito J" first="José" last="Lima-Brito">José Lima-Brito</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">25429047</idno><idno type="pmc">4294445</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4294445</idno><idno type="RBID">PMC:4294445</idno><idno type="doi">10.1093/aobpla/plu079</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000008</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000008</idno><idno type="wicri:Area/Pmc/Curation">000008</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000008</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism</title><author><name sortKey="Coutinho, Joao Paulo" sort="Coutinho, Joao Paulo" uniqKey="Coutinho J" first="João Paulo" last="Coutinho">João Paulo Coutinho</name></author><author><name sortKey="Carvalho, Ana" sort="Carvalho, Ana" uniqKey="Carvalho A" first="Ana" last="Carvalho">Ana Carvalho</name></author><author><name sortKey="Lima Brito, Jose" sort="Lima Brito, Jose" uniqKey="Lima Brito J" first="José" last="Lima-Brito">José Lima-Brito</name></author></analytic><series><title level="j">AoB Plants</title><idno type="eISSN">2041-2851</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In 30 Fagaceae individuals from the <italic>Castanea</italic>, <italic>Fagus</italic> and <italic>Quercus</italic> genera, the internal transcribed spacer region (ITS) of the ribosomal DNA (rDNA) was amplified and digested with four restriction enzymes (<italic>Hae</italic>III, <italic>Hpa</italic>II, <italic>Sau</italic>96I, and <italic>Taq</italic>I), producing ITS PCR-RFLP markers. This technique allowed the discrimination of the Fagaceae species according to genus, infrageneric group and ecological area. This study constitutes the first application of the alternative co-dominant marker system ITS PCR-RFLP in Fagaceae species and proved to be highly suitable for taxonomic and ecological discrimination, constituting a useful molecular tool for taxonomists and forestry researchers.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Baldwin, Bg" uniqKey="Baldwin B">BG Baldwin</name></author><author><name sortKey="Sanderson, Mj" uniqKey="Sanderson M">MJ Sanderson</name></author><author><name sortKey="Porter, Jm" uniqKey="Porter J">JM Porter</name></author><author><name sortKey="Wojeiechowski, Mf" uniqKey="Wojeiechowski M">MF Wojeiechowski</name></author><author><name sortKey="Capbell, Cs" uniqKey="Capbell C">CS Capbell</name></author><author><name sortKey="Donoghe, Mj" uniqKey="Donoghe M">MJ Donoghe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belahbib, N" uniqKey="Belahbib N">N Belahbib</name></author><author><name sortKey="Pemonge, Mh" uniqKey="Pemonge M">MH Pemonge</name></author><author><name sortKey="Ouassou, A" uniqKey="Ouassou A">A Ouassou</name></author><author><name sortKey="Sbay, H" uniqKey="Sbay H">H Sbay</name></author><author><name sortKey="Kremer, A" uniqKey="Kremer A">A Kremer</name></author><author><name sortKey="Petit, Rj" uniqKey="Petit R">RJ Petit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bellarosa, R" uniqKey="Bellarosa R">R Bellarosa</name></author><author><name sortKey="Simeone, Mc" uniqKey="Simeone M">MC Simeone</name></author><author><name sortKey="Papini, A" uniqKey="Papini A">A Papini</name></author><author><name sortKey="Schirone, B" uniqKey="Schirone B">B Schirone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Camus, A" uniqKey="Camus A">A Camus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Polanco, C" uniqKey="Polanco C">C Polanco</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Polanco, C" uniqKey="Polanco C">C Polanco</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casas, Rr" uniqKey="Casas R">RR Casas</name></author><author><name sortKey="Cano, E" uniqKey="Cano E">E Cano</name></author><author><name sortKey="Balaguer, L" uniqKey="Balaguer L">L Balaguer</name></author><author><name sortKey="Perez Corona, E" uniqKey="Perez Corona E">E Pérez-Corona</name></author><author><name sortKey="Manrique, E" uniqKey="Manrique E">E Manrique</name></author><author><name sortKey="Garcia Verdugo, C" uniqKey="Garcia Verdugo C">C García-Verdugo</name></author><author><name sortKey="Vargas, P" uniqKey="Vargas P">P Vargas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Conte, L" uniqKey="Conte L">L Conte</name></author><author><name sortKey="Cotti, C" uniqKey="Cotti C">C Cotti</name></author><author><name sortKey="Cristofolini, G" uniqKey="Cristofolini G">G Cristofolini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cottrell, Je" uniqKey="Cottrell J">JE Cottrell</name></author><author><name sortKey="Munro, Rc" uniqKey="Munro R">RC Munro</name></author><author><name sortKey="Tabbener, He" uniqKey="Tabbener H">HE Tabbener</name></author><author><name sortKey="Gillies, Acm" uniqKey="Gillies A">ACM Gillies</name></author><author><name sortKey="Forrest, Gi" uniqKey="Forrest G">GI Forrest</name></author><author><name sortKey="Deans, Jd" uniqKey="Deans J">JD Deans</name></author><author><name sortKey="Lowe, Aj" uniqKey="Lowe A">AJ Lowe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coutinho, Jp" uniqKey="Coutinho J">JP Coutinho</name></author><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Csaikl, Um" uniqKey="Csaikl U">UM Csaikl</name></author><author><name sortKey="Burg, K" uniqKey="Burg K">K Burg</name></author><author><name sortKey="Fineschi, S" uniqKey="Fineschi S">S Fineschi</name></author><author><name sortKey="Konig, Ao" uniqKey="Konig A">AO König</name></author><author><name sortKey="Matyas, G" uniqKey="Matyas G">G Mátyás</name></author><author><name sortKey="Petit, Rj" uniqKey="Petit R">RJ Petit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denk, T" uniqKey="Denk T">T Denk</name></author><author><name sortKey="Grimm, Gw" uniqKey="Grimm G">GW Grimm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denk, T" uniqKey="Denk T">T Denk</name></author><author><name sortKey="Grimm, Gw" uniqKey="Grimm G">GW Grimm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denk, T" uniqKey="Denk T">T Denk</name></author><author><name sortKey="Grimm, G" uniqKey="Grimm G">G Grimm</name></author><author><name sortKey="Stogerer, K" uniqKey="Stogerer K">K Stögerer</name></author><author><name sortKey="Langer, M" uniqKey="Langer M">M Langer</name></author><author><name sortKey="Hemleben, V" uniqKey="Hemleben V">V Hemleben</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denk, T" uniqKey="Denk T">T Denk</name></author><author><name sortKey="Grimsson, F" uniqKey="Grimsson F">F Grímsson</name></author><author><name sortKey="Zetter, R" uniqKey="Zetter R">R Zetter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Denk, T" uniqKey="Denk T">T Denk</name></author><author><name sortKey="Grimsson, F" uniqKey="Grimsson F">F Grímsson</name></author><author><name sortKey="Zetter, R" uniqKey="Zetter R">R Zetter</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dover, Ga" uniqKey="Dover G">GA Dover</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dover, Ga" uniqKey="Dover G">GA Dover</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doyle, Jj" uniqKey="Doyle J">JJ Doyle</name></author><author><name sortKey="Doyle, Jl" uniqKey="Doyle J">JL Doyle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Felsenstein, J" uniqKey="Felsenstein J">J Felsenstein</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gonzalez Rodriguez, A" uniqKey="Gonzalez Rodriguez A">A González-Rodríguez</name></author><author><name sortKey="Bain, Jf" uniqKey="Bain J">JF Bain</name></author><author><name sortKey="Golden, Jl" uniqKey="Golden J">JL Golden</name></author><author><name sortKey="Oyama, K" uniqKey="Oyama K">K Oyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harpke, D" uniqKey="Harpke D">D Harpke</name></author><author><name sortKey="Peterson, A" uniqKey="Peterson A">A Peterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jimenez, P" uniqKey="Jimenez P">P Jiménez</name></author><author><name sortKey="Heredia, Ul" uniqKey="Heredia U">UL Heredia</name></author><author><name sortKey="Collada, C" uniqKey="Collada C">C Collada</name></author><author><name sortKey="Lorenzo, Z" uniqKey="Lorenzo Z">Z Lorenzo</name></author><author><name sortKey="Gil, L" uniqKey="Gil L">L Gil</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lang, P" uniqKey="Lang P">P Lang</name></author><author><name sortKey="Dane, F" uniqKey="Dane F">F Dane</name></author><author><name sortKey="Kubisiak, Tl" uniqKey="Kubisiak T">TL Kubisiak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lang, P" uniqKey="Lang P">P Lang</name></author><author><name sortKey="Dane, F" uniqKey="Dane F">F Dane</name></author><author><name sortKey="Kubisiak, Tl" uniqKey="Kubisiak T">TL Kubisiak</name></author><author><name sortKey="Huang, H" uniqKey="Huang H">H Huang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lewontin, Rc" uniqKey="Lewontin R">RC Lewontin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lima Brito, J" uniqKey="Lima Brito J">J Lima-Brito</name></author><author><name sortKey="Castro, L" uniqKey="Castro L">L Castro</name></author><author><name sortKey="Coutinho, J" uniqKey="Coutinho J">J Coutinho</name></author><author><name sortKey="Morais, F" uniqKey="Morais F">F Morais</name></author><author><name sortKey="Gomes, L" uniqKey="Gomes L">L Gomes</name></author><author><name sortKey="Guedes Pinto, H" uniqKey="Guedes Pinto H">H Guedes-Pinto</name></author><author><name sortKey="Carvalho, A" uniqKey="Carvalho A">A Carvalho</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lumaret, R" uniqKey="Lumaret R">R Lumaret</name></author><author><name sortKey="Tryphon Dionnet, M" uniqKey="Tryphon Dionnet M">M Tryphon-Dionnet</name></author><author><name sortKey="Michaud, H" uniqKey="Michaud H">H Michaud</name></author><author><name sortKey="Sanuy, A" uniqKey="Sanuy A">A Sanuy</name></author><author><name sortKey="Ipotesi, E" uniqKey="Ipotesi E">E Ipotesi</name></author><author><name sortKey="Born, C" uniqKey="Born C">C Born</name></author><author><name sortKey="Mir, C" uniqKey="Mir C">C Mir</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manos, Ps" uniqKey="Manos P">PS Manos</name></author><author><name sortKey="Doyle, Jj" uniqKey="Doyle J">JJ Doyle</name></author><author><name sortKey="Nixon, Kc" uniqKey="Nixon K">KC Nixon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Manos, Ps" uniqKey="Manos P">PS Manos</name></author><author><name sortKey="Zhou, Zk" uniqKey="Zhou Z">ZK Zhou</name></author><author><name sortKey="Cannon, Ch" uniqKey="Cannon C">CH Cannon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mayol, M" uniqKey="Mayol M">M Mayol</name></author><author><name sortKey="Rossell, Ja" uniqKey="Rossell J">JA Rosselló</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mcclintock, B" uniqKey="Mcclintock B">B McClintock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muir, G" uniqKey="Muir G">G Muir</name></author><author><name sortKey="Schlotterer, C" uniqKey="Schlotterer C">C Schlötterer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Muir, G" uniqKey="Muir G">G Muir</name></author><author><name sortKey="Fleming, Cc" uniqKey="Fleming C">CC Fleming</name></author><author><name sortKey="Schlotterer, C" uniqKey="Schlotterer C">C Schlötterer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nalini, E" uniqKey="Nalini E">E Nalini</name></author><author><name sortKey="Bhagwat, Sg" uniqKey="Bhagwat S">SG Bhagwat</name></author><author><name sortKey="Jawali, N" uniqKey="Jawali N">N Jawali</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Navashin, M" uniqKey="Navashin M">M Navashin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nixon, Kc" uniqKey="Nixon K">KC Nixon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nwakanma, Dc" uniqKey="Nwakanma D">DC Nwakanma</name></author><author><name sortKey="Pillay, M" uniqKey="Pillay M">M Pillay</name></author><author><name sortKey="Okoli, Be" uniqKey="Okoli B">BE Okoli</name></author><author><name sortKey="Tenkouano, A" uniqKey="Tenkouano A">A Tenkouano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Page, Rdm" uniqKey="Page R">RDM Page</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Papini, A" uniqKey="Papini A">A Papini</name></author><author><name sortKey="Simeone, Mc" uniqKey="Simeone M">MC Simeone</name></author><author><name sortKey="Bellarosa, R" uniqKey="Bellarosa R">R Bellarosa</name></author><author><name sortKey="Spada, F" uniqKey="Spada F">F Spada</name></author><author><name sortKey="Schirone, B" uniqKey="Schirone B">B Schirone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pritchard, Jk" uniqKey="Pritchard J">JK Pritchard</name></author><author><name sortKey="Stephens, M" uniqKey="Stephens M">M Stephens</name></author><author><name sortKey="Donnelly, P" uniqKey="Donnelly P">P Donnelly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pritchard, Jk" uniqKey="Pritchard J">JK Pritchard</name></author><author><name sortKey="Wen, X" uniqKey="Wen X">X Wen</name></author><author><name sortKey="Falush, D" uniqKey="Falush D">D Falush</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Quijada, A" uniqKey="Quijada A">A Quijada</name></author><author><name sortKey="Liston, A" uniqKey="Liston A">A Liston</name></author><author><name sortKey="Delgado, P" uniqKey="Delgado P">P Delgado</name></author><author><name sortKey="Vazquez Lobo, A" uniqKey="Vazquez Lobo A">A Vázquez-Lobo</name></author><author><name sortKey="Alvarez Buylla, Er" uniqKey="Alvarez Buylla E">ER Alvarez-Buylla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Redecker, D" uniqKey="Redecker D">D Redecker</name></author><author><name sortKey="Thierfelder, H" uniqKey="Thierfelder H">H Thierfelder</name></author><author><name sortKey="Walker, C" uniqKey="Walker C">C Walker</name></author><author><name sortKey="Werner, A" uniqKey="Werner A">A Werner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rohlf, Fj" uniqKey="Rohlf F">FJ Rohlf</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saini, A" uniqKey="Saini A">A Saini</name></author><author><name sortKey="Reddy, Sk" uniqKey="Reddy S">SK Reddy</name></author><author><name sortKey="Jawali, N" uniqKey="Jawali N">N Jawali</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saitou, N" uniqKey="Saitou N">N Saitou</name></author><author><name sortKey="Nei, M" uniqKey="Nei M">M Nei</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Samuel, R" uniqKey="Samuel R">R Samuel</name></author><author><name sortKey="Bachmair, A" uniqKey="Bachmair A">A Bachmair</name></author><author><name sortKey="Jobst, J" uniqKey="Jobst J">J Jobst</name></author><author><name sortKey="Ehrendorfer, F" uniqKey="Ehrendorfer F">F Ehrendorfer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thiers, B" uniqKey="Thiers B">B Thiers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="White, Tj" uniqKey="White T">TJ White</name></author><author><name sortKey="Bruns, T" uniqKey="Bruns T">T Bruns</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S Lee</name></author><author><name sortKey="Taylor, J" uniqKey="Taylor J">J Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yap, Iv" uniqKey="Yap I">IV Yap</name></author><author><name sortKey="Nelson, Rj" uniqKey="Nelson R">RJ Nelson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeh, Fc" uniqKey="Yeh F">FC Yeh</name></author><author><name sortKey="Yang, Rc" uniqKey="Yang R">RC Yang</name></author><author><name sortKey="Boyle, Tbj" uniqKey="Boyle T">TBJ Boyle</name></author><author><name sortKey="Ye, Zh" uniqKey="Ye Z">ZH Ye</name></author><author><name sortKey="Mao, Jx" uniqKey="Mao J">JX Mao</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">AoB Plants</journal-id><journal-id journal-id-type="iso-abbrev">AoB Plants</journal-id><journal-id journal-id-type="publisher-id">aobpla</journal-id><journal-id journal-id-type="hwp">aobpla</journal-id><journal-title-group><journal-title>AoB Plants</journal-title></journal-title-group><issn pub-type="epub">2041-2851</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">25429047</article-id><article-id pub-id-type="pmc">4294445</article-id><article-id pub-id-type="doi">10.1093/aobpla/plu079</article-id><article-id pub-id-type="publisher-id">plu079</article-id><article-categories><subj-group subj-group-type="hwp-journal-coll"><subject>1013</subject><subject>1008</subject></subj-group><subj-group subj-group-type="heading"><subject>Research Articles</subject></subj-group></article-categories><title-group><article-title>Taxonomic and ecological discrimination of Fagaceae species based on internal transcribed spacer polymerase chain reaction–restriction fragment length polymorphism</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Coutinho</surname><given-names>João Paulo</given-names></name></contrib><contrib contrib-type="author"><name><surname>Carvalho</surname><given-names>Ana</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lima-Brito</surname><given-names>José</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff><institution>Institute of Biotechnology and Bioengineering (IBB), Centre of Genomics and Biotechnology (CGB), University of Tras-os-Montes and</institution><addr-line>Alto Douro, 5001-801 Vila Real</addr-line>,<country>Portugal</country></aff></contrib-group><author-notes><corresp id="cor1"><label>*</label>Corresponding author's e-mail address: <email>jbrito@utad.pt</email></corresp><fn fn-type="con" id="an1"><p><bold>Associate Editor:</bold> Kermit Ritland</p></fn></author-notes><pub-date pub-type="collection"><year>2015</year></pub-date><pub-date pub-type="epub"><day>26</day><month>11</month><year>2014</year></pub-date><volume>7</volume><elocation-id>plu079</elocation-id><history><date date-type="received"><day>30</day><month>8</month><year>2014</year></date><date date-type="accepted"><day>15</day><month>11</month><year>2014</year></date></history><permissions><copyright-statement>Published by Oxford University Press on behalf of the Annals of Botany Company.</copyright-statement><copyright-year>2014</copyright-year><license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by/4.0/"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<uri xlink:type="simple" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</uri>), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><self-uri content-type="pdf" xlink:type="simple" xlink:href="plu079.pdf"></self-uri><abstract abstract-type="precis"><p>In 30 Fagaceae individuals from the <italic>Castanea</italic>, <italic>Fagus</italic> and <italic>Quercus</italic> genera, the internal transcribed spacer region (ITS) of the ribosomal DNA (rDNA) was amplified and digested with four restriction enzymes (<italic>Hae</italic>III, <italic>Hpa</italic>II, <italic>Sau</italic>96I, and <italic>Taq</italic>I), producing ITS PCR-RFLP markers. This technique allowed the discrimination of the Fagaceae species according to genus, infrageneric group and ecological area. This study constitutes the first application of the alternative co-dominant marker system ITS PCR-RFLP in Fagaceae species and proved to be highly suitable for taxonomic and ecological discrimination, constituting a useful molecular tool for taxonomists and forestry researchers.</p></abstract><abstract><p>The internal transcribed spacer (ITS) of ribosomal DNA has been used to confirm taxonomic classifications and define phylogenies in several plant species following sequencing or polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) techniques. In this study, co-dominant ITS PCR–RFLP molecular markers were produced in 30 Fagaceae individuals belonging to the <italic>Castanea</italic>, <italic>Fagus</italic> and <italic>Quercus</italic> genera in order to assess the potential of this technique for taxonomic discrimination and determination of phylogenies. The complete ITS region (ITS1-5.8S rRNA-ITS2) was amplified in most of the Fagaceae individuals as a single fragment of ∼700 bp. The ITS amplified products were digested with nine restriction enzymes, but only four (HaeIII, HpaII, TaqI and Sau96I) produced polymorphic/discriminative patterns. The total expected heterozygosity (<italic>H</italic><sub>E</sub>) was 20.31 % and the gene diversity (<italic>I</italic>), 32.97 %. The ITS polymorphism was higher within the <italic>Quercus</italic> genus (85.3 %). The ITS PCR–RFLP markers clustered the Fagaceae species according to genus or infrageneric group (in the case of <italic>Quercus</italic> sp. individuals). Five oaks did not cluster in line with the adopted infrageneric classification, but three of these were grouped according to their actual ecological distributions. The ITS PCR–RFLP markers indicated their potential for phylogenetic studies since all Fagaceae individuals were discriminated according to genus, and most of the oaks were clustered according to infrageneric group or ecological area.</p></abstract><kwd-group><kwd><italic>Castanea</italic></kwd><kwd><italic>Fagus</italic></kwd><kwd>internal transcribed spacer (ITS)</kwd><kwd><italic>Quercus</italic></kwd><kwd>restriction fragment length polymorphism (RFLP).</kwd></kwd-group><counts><page-count count="13"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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