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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Binding Site for <italic>Bacillus thuringiensis</italic> Cry1Ab Toxin Is Lost during Larval Development in Two Forest Pests</title><author><name sortKey="Rausell, Carolina" sort="Rausell, Carolina" uniqKey="Rausell C" first="Carolina" last="Rausell">Carolina Rausell</name></author><author><name sortKey="Martinez Ramirez, Amparo Consuelo" sort="Martinez Ramirez, Amparo Consuelo" uniqKey="Martinez Ramirez A" first="Amparo Consuelo" last="Martínez-Ramírez">Amparo Consuelo Martínez-Ramírez</name></author><author><name sortKey="Garcia Robles, Inmaculada" sort="Garcia Robles, Inmaculada" uniqKey="Garcia Robles I" first="Inmaculada" last="García-Robles">Inmaculada García-Robles</name></author><author><name sortKey="Real, Maria Dolores" sort="Real, Maria Dolores" uniqKey="Real M" first="María Dolores" last="Real">María Dolores Real</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">10742241</idno><idno type="pmc">92022</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92022</idno><idno type="RBID">PMC:92022</idno><date when="2000">2000</date><idno type="wicri:Area/Pmc/Corpus">000060</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000060</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Binding Site for <italic>Bacillus thuringiensis</italic> Cry1Ab Toxin Is Lost during Larval Development in Two Forest Pests</title><author><name sortKey="Rausell, Carolina" sort="Rausell, Carolina" uniqKey="Rausell C" first="Carolina" last="Rausell">Carolina Rausell</name></author><author><name sortKey="Martinez Ramirez, Amparo Consuelo" sort="Martinez Ramirez, Amparo Consuelo" uniqKey="Martinez Ramirez A" first="Amparo Consuelo" last="Martínez-Ramírez">Amparo Consuelo Martínez-Ramírez</name></author><author><name sortKey="Garcia Robles, Inmaculada" sort="Garcia Robles, Inmaculada" uniqKey="Garcia Robles I" first="Inmaculada" last="García-Robles">Inmaculada García-Robles</name></author><author><name sortKey="Real, Maria Dolores" sort="Real, Maria Dolores" uniqKey="Real M" first="María Dolores" last="Real">María Dolores Real</name></author></analytic><series><title level="j">Applied and Environmental Microbiology</title><idno type="ISSN">0099-2240</idno><idno type="eISSN">1098-5336</idno><imprint><date when="2000">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The insecticidal activity and receptor binding properties of <italic>Bacillus thuringiensis</italic> Cry1A toxins towards the forest pests <italic>Thaumetopoea pityocampa</italic> (processionary moth) and <italic>Lymantria monacha</italic> (nun moth) were investigated. Cry1Aa, Cry1Ab, and Cry1Ac were highly toxic (corresponding 50% lethal concentration values: 956, 895, and 379 pg/μl, respectively) to first-instar <italic>T. pityocampa</italic> larvae. During larval development, Cry1Ab and Cry1Ac toxicity decreased with increasing age, although the loss of activity was more pronounced for Cry1Ab. Binding assays with <sup>125</sup>I-labelled Cry1Ab and brush border membrane vesicles from <italic>T. pityocampa</italic> first- and last-instar larvae detected a remarkable decrease in the overall Cry1Ab binding affinity in last-instar larvae, although saturable Cry1Ab binding to both instars was observed. Homologous competition experiments demonstrated the loss of one of the two Cry1Ab high-affinity binding sites detected in first-instar larvae. Growth inhibition assays with sublethal doses of Cry1Aa, Cry1Ab, and Cry1Ac in <italic>L. monacha</italic> showed that all three toxins were able to delay molting from second instar to third instar. Specific saturable binding of Cry1Ab was detected only in first- and second-instar larvae. Cry1Ab binding was not detected in last-instar larvae, although specific binding of Cry1Aa and Cry1Ac was observed. These results demonstrate a loss of Cry1Ab binding sites during development on the midgut epithelium of <italic>T. pityocampa</italic> and <italic>L. monacha</italic>, correlating in <italic>T. pityocampa</italic> with a decrease in Cry1Ab toxicity with increasing age.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Appl Environ Microbiol</journal-id><journal-id journal-id-type="publisher-id">APPL ENVIRON MICROBIOL</journal-id><journal-title>Applied and Environmental Microbiology</journal-title><issn pub-type="ppub">0099-2240</issn><issn pub-type="epub">1098-5336</issn><publisher><publisher-name>American Society for Microbiology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">10742241</article-id><article-id pub-id-type="pmc">92022</article-id><article-id pub-id-type="publisher-id">1003</article-id><article-categories><subj-group subj-group-type="heading"><subject>Invertebrate Microbiology</subject></subj-group></article-categories><title-group><article-title>A Binding Site for <italic>Bacillus thuringiensis</italic> Cry1Ab Toxin Is Lost during Larval Development in Two Forest Pests</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Rausell</surname><given-names>Carolina</given-names></name></contrib><contrib contrib-type="author"><name><surname>Martínez-Ramírez</surname><given-names>Amparo Consuelo</given-names></name></contrib><contrib contrib-type="author"><name><surname>García-Robles</surname><given-names>Inmaculada</given-names></name></contrib><contrib contrib-type="author"><name><surname>Real</surname><given-names>María Dolores</given-names></name><xref ref-type="author-notes" rid="FN150">*</xref></contrib></contrib-group><aff id="N0x986d2b0.0x9c0e1f0">Departamento de Genética, Facultad de Ciencias Biológicas, 46100-Burjassot (Valencia), Spain</aff><author-notes><fn id="FN150"><label>*</label><p>Corresponding author. Mailing address: Departamento de Genética (Facultad de Ciencias Biológicas), Doctor Moliner, 50, 46100-Burjassot (Valencia), Spain. Phone: 34 96 398 30 28. Fax: 34 96 398 30 29. E-mail: <email>realmd@uv.es</email>.</p></fn></author-notes><pub-date pub-type="ppub"><month>4</month><year>2000</year></pub-date><volume>66</volume><issue>4</issue><fpage>1553</fpage><lpage>1558</lpage><history><date date-type="received"><day>28</day><month>6</month><year>1999</year></date><date date-type="accepted"><day>27</day><month>1</month><year>2000</year></date></history><copyright-statement>Copyright © 2000, American Society for Microbiology</copyright-statement><copyright-year>2000</copyright-year><abstract><p>The insecticidal activity and receptor binding properties of <italic>Bacillus thuringiensis</italic> Cry1A toxins towards the forest pests <italic>Thaumetopoea pityocampa</italic> (processionary moth) and <italic>Lymantria monacha</italic> (nun moth) were investigated. Cry1Aa, Cry1Ab, and Cry1Ac were highly toxic (corresponding 50% lethal concentration values: 956, 895, and 379 pg/μl, respectively) to first-instar <italic>T. pityocampa</italic> larvae. During larval development, Cry1Ab and Cry1Ac toxicity decreased with increasing age, although the loss of activity was more pronounced for Cry1Ab. Binding assays with <sup>125</sup>I-labelled Cry1Ab and brush border membrane vesicles from <italic>T. pityocampa</italic> first- and last-instar larvae detected a remarkable decrease in the overall Cry1Ab binding affinity in last-instar larvae, although saturable Cry1Ab binding to both instars was observed. Homologous competition experiments demonstrated the loss of one of the two Cry1Ab high-affinity binding sites detected in first-instar larvae. Growth inhibition assays with sublethal doses of Cry1Aa, Cry1Ab, and Cry1Ac in <italic>L. monacha</italic> showed that all three toxins were able to delay molting from second instar to third instar. Specific saturable binding of Cry1Ab was detected only in first- and second-instar larvae. Cry1Ab binding was not detected in last-instar larvae, although specific binding of Cry1Aa and Cry1Ac was observed. These results demonstrate a loss of Cry1Ab binding sites during development on the midgut epithelium of <italic>T. pityocampa</italic> and <italic>L. monacha</italic>, correlating in <italic>T. pityocampa</italic> with a decrease in Cry1Ab toxicity with increasing age.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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