Molecular and Genetic Evidence for a Tetrapolar Mating System in the Basidiomycetous Yeast Kwoniella mangrovensis and Two Novel Sibling Species
Identifieur interne : 000133 ( Pmc/Checkpoint ); précédent : 000132; suivant : 000134Molecular and Genetic Evidence for a Tetrapolar Mating System in the Basidiomycetous Yeast Kwoniella mangrovensis and Two Novel Sibling Species
Auteurs : Marco A. Guerreiro ; Deborah J. Springer ; Joana A. Rodrigues ; Laura N. Rusche ; Keisha Findley ; Joseph Heitman ; Álvaro FonsecaSource :
- Eukaryotic Cell [ 1535-9778 ] ; 2013.
Abstract
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DOI: 10.1128/EC.00065-13
PubMed: 23524993
PubMed Central: 3647770
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular and Genetic Evidence for a Tetrapolar Mating System in the Basidiomycetous Yeast <named-content content-type="genus-species">Kwoniella mangrovensis</named-content> and Two Novel Sibling Species</title><author><name sortKey="Guerreiro, Marco A" sort="Guerreiro, Marco A" uniqKey="Guerreiro M" first="Marco A." last="Guerreiro">Marco A. Guerreiro</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Springer, Deborah J" sort="Springer, Deborah J" uniqKey="Springer D" first="Deborah J." last="Springer">Deborah J. Springer</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Rodrigues, Joana A" sort="Rodrigues, Joana A" uniqKey="Rodrigues J" first="Joana A." last="Rodrigues">Joana A. Rodrigues</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rusche, Laura N" sort="Rusche, Laura N" uniqKey="Rusche L" first="Laura N." last="Rusche">Laura N. Rusche</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Findley, Keisha" sort="Findley, Keisha" uniqKey="Findley K" first="Keisha" last="Findley">Keisha Findley</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Heitman, Joseph" sort="Heitman, Joseph" uniqKey="Heitman J" first="Joseph" last="Heitman">Joseph Heitman</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Fonseca, Alvaro" sort="Fonseca, Alvaro" uniqKey="Fonseca A" first="Álvaro" last="Fonseca">Álvaro Fonseca</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23524993</idno><idno type="pmc">3647770</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3647770</idno><idno type="RBID">PMC:3647770</idno><idno type="doi">10.1128/EC.00065-13</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000144</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000144</idno><idno type="wicri:Area/Pmc/Curation">000144</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000144</idno><idno type="wicri:Area/Pmc/Checkpoint">000133</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000133</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular and Genetic Evidence for a Tetrapolar Mating System in the Basidiomycetous Yeast <named-content content-type="genus-species">Kwoniella mangrovensis</named-content> and Two Novel Sibling Species</title><author><name sortKey="Guerreiro, Marco A" sort="Guerreiro, Marco A" uniqKey="Guerreiro M" first="Marco A." last="Guerreiro">Marco A. Guerreiro</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Springer, Deborah J" sort="Springer, Deborah J" uniqKey="Springer D" first="Deborah J." last="Springer">Deborah J. Springer</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Rodrigues, Joana A" sort="Rodrigues, Joana A" uniqKey="Rodrigues J" first="Joana A." last="Rodrigues">Joana A. Rodrigues</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rusche, Laura N" sort="Rusche, Laura N" uniqKey="Rusche L" first="Laura N." last="Rusche">Laura N. Rusche</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Findley, Keisha" sort="Findley, Keisha" uniqKey="Findley K" first="Keisha" last="Findley">Keisha Findley</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Heitman, Joseph" sort="Heitman, Joseph" uniqKey="Heitman J" first="Joseph" last="Heitman">Joseph Heitman</name><affiliation><nlm:aff id="aff2"></nlm:aff></affiliation></author><author><name sortKey="Fonseca, Alvaro" sort="Fonseca, Alvaro" uniqKey="Fonseca A" first="Álvaro" last="Fonseca">Álvaro Fonseca</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Eukaryotic Cell</title><idno type="ISSN">1535-9778</idno><idno type="eISSN">1535-9786</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><named-content content-type="genus-species">Kwoniella mangrovensis</named-content> has been described as a sexual species with a bipolar mating system. Phylogenetic analysis of multiple genes places this species together with <named-content content-type="genus-species">Kwoniella heveanensis</named-content> in the <named-content content-type="genus-species">Kwoniella</named-content> clade, a sister clade to that containing two pathogenic species of global importance, <named-content content-type="genus-species">Cryptococcus neoformans</named-content> and <named-content content-type="genus-species">Cryptococcus gattii</named-content>, within the Tremellales. Recent studies defining the mating type loci (<italic>MAT</italic>) of species in these clades showed that, with the exception of <named-content content-type="genus-species">C. neoformans</named-content> and <named-content content-type="genus-species">C. gattii</named-content>, which are bipolar with a single biallelic multigene <italic>MAT</italic> locus, several other species feature a tetrapolar mating system with two unlinked loci (homeodomain [HD] and pheromone/receptor [P/R] loci). We characterized several strains from the original study describing <named-content content-type="genus-species">K. mangrovensis</named-content>; two <italic>MAT</italic> regions were amplified and sequenced: the <italic>STE20</italic> gene (P/R locus) and the divergently transcribed <italic>SXI1</italic> and <italic>SXI2</italic> genes (HD locus). We identified five different mating types with different <italic>STE20</italic>/<italic>SXI</italic> allele combinations that together with results of mating experiments demonstrate that <named-content content-type="genus-species">K. mangrovensis</named-content> is not bipolar but instead has a tetrapolar mating system. Sequence and gene analysis for a 43-kb segment of the <named-content content-type="genus-species">K. mangrovensis</named-content> type strain <italic>MAT</italic> locus revealed remarkable synteny with the homologous <named-content content-type="genus-species">K. heveanensis</named-content><italic>MAT</italic> P/R region, providing new insights into slower evolution of <italic>MAT</italic> loci in the <named-content content-type="genus-species">Kwoniella</named-content> compared to the <named-content content-type="genus-species">Cryptococcus</named-content> clade of the Tremellales. The study of additional isolates from plant substrates in Europe and Botswana using a combination of multilocus sequencing with <italic>MAT</italic> gene analysis revealed two novel sibling species that we name <named-content content-type="genus-species">Kwoniella europaea</named-content> and <named-content content-type="genus-species">Kwoniella botswanensis</named-content> and which appear to also have tetrapolar mating systems.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Eukaryot Cell</journal-id><journal-id journal-id-type="iso-abbrev">Eukaryotic Cell</journal-id><journal-id journal-id-type="hwp">eukcell</journal-id><journal-id journal-id-type="pmc">eukcell</journal-id><journal-id journal-id-type="publisher-id">EUKCELL</journal-id><journal-title-group><journal-title>Eukaryotic Cell</journal-title></journal-title-group><issn pub-type="ppub">1535-9778</issn><issn pub-type="epub">1535-9786</issn><publisher><publisher-name>American Society for Microbiology</publisher-name><publisher-loc>1752 N St., N.W., Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23524993</article-id><article-id pub-id-type="pmc">3647770</article-id><article-id pub-id-type="publisher-id">00065-13</article-id><article-id pub-id-type="doi">10.1128/EC.00065-13</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>Molecular and Genetic Evidence for a Tetrapolar Mating System in the Basidiomycetous Yeast <named-content content-type="genus-species">Kwoniella mangrovensis</named-content> and Two Novel Sibling Species</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Guerreiro</surname><given-names>Marco A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><contrib contrib-type="author"><name><surname>Springer</surname><given-names>Deborah J.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author"><name><surname>Rodrigues</surname><given-names>Joana A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Rusche</surname><given-names>Laura N.</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Findley</surname><given-names>Keisha</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib><contrib contrib-type="author"><name><surname>Heitman</surname><given-names>Joseph</given-names></name><xref ref-type="aff" rid="aff2"><sup>b</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Fonseca</surname><given-names>Álvaro</given-names></name><xref ref-type="aff" rid="aff1"><sup>a</sup></xref></contrib><aff id="aff1">Centro de Recursos Microbiológicos (CREM), Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica, Portugal<label>a</label></aff><aff id="aff2">Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, North Carolina, USA<label>b</label></aff></contrib-group><author-notes><corresp>Address correspondence to Álvaro Fonseca, <email>amrf@fct.unl.pt</email>.</corresp><fn id="FN1" fn-type="present-address"><label>*</label><p>Present address: Joana A. Rodrigues, Departamento das Ciências e Tecnologia da Biomassa, FCT/UNL, Caparica, Portugal; Laura N. Rusche, Department of Biological Sciences, University at Buffalo, SUNY, Buffalo, New York, USA; Keisha Findley, National Human Genome Research Institute, Bethesda, Maryland, USA.</p></fn></author-notes><pub-date pub-type="ppub"><month>5</month><year>2013</year></pub-date><volume>12</volume><issue>5</issue><fpage>746</fpage><lpage>760</lpage><history><date date-type="received"><day>9</day><month>3</month><year>2013</year></date><date date-type="accepted"><day>15</day><month>3</month><year>2013</year></date></history><permissions><copyright-statement>Copyright © 2013, American Society for Microbiology. All Rights Reserved.</copyright-statement><copyright-year>2013</copyright-year><copyright-holder>American Society for Microbiology</copyright-holder></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="zek00513000746.pdf"></self-uri><abstract><p><named-content content-type="genus-species">Kwoniella mangrovensis</named-content> has been described as a sexual species with a bipolar mating system. Phylogenetic analysis of multiple genes places this species together with <named-content content-type="genus-species">Kwoniella heveanensis</named-content> in the <named-content content-type="genus-species">Kwoniella</named-content> clade, a sister clade to that containing two pathogenic species of global importance, <named-content content-type="genus-species">Cryptococcus neoformans</named-content> and <named-content content-type="genus-species">Cryptococcus gattii</named-content>, within the Tremellales. Recent studies defining the mating type loci (<italic>MAT</italic>) of species in these clades showed that, with the exception of <named-content content-type="genus-species">C. neoformans</named-content> and <named-content content-type="genus-species">C. gattii</named-content>, which are bipolar with a single biallelic multigene <italic>MAT</italic> locus, several other species feature a tetrapolar mating system with two unlinked loci (homeodomain [HD] and pheromone/receptor [P/R] loci). We characterized several strains from the original study describing <named-content content-type="genus-species">K. mangrovensis</named-content>; two <italic>MAT</italic> regions were amplified and sequenced: the <italic>STE20</italic> gene (P/R locus) and the divergently transcribed <italic>SXI1</italic> and <italic>SXI2</italic> genes (HD locus). We identified five different mating types with different <italic>STE20</italic>/<italic>SXI</italic> allele combinations that together with results of mating experiments demonstrate that <named-content content-type="genus-species">K. mangrovensis</named-content> is not bipolar but instead has a tetrapolar mating system. Sequence and gene analysis for a 43-kb segment of the <named-content content-type="genus-species">K. mangrovensis</named-content> type strain <italic>MAT</italic> locus revealed remarkable synteny with the homologous <named-content content-type="genus-species">K. heveanensis</named-content><italic>MAT</italic> P/R region, providing new insights into slower evolution of <italic>MAT</italic> loci in the <named-content content-type="genus-species">Kwoniella</named-content> compared to the <named-content content-type="genus-species">Cryptococcus</named-content> clade of the Tremellales. The study of additional isolates from plant substrates in Europe and Botswana using a combination of multilocus sequencing with <italic>MAT</italic> gene analysis revealed two novel sibling species that we name <named-content content-type="genus-species">Kwoniella europaea</named-content> and <named-content content-type="genus-species">Kwoniella botswanensis</named-content> and which appear to also have tetrapolar mating systems.</p></abstract></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Findley, Keisha" sort="Findley, Keisha" uniqKey="Findley K" first="Keisha" last="Findley">Keisha Findley</name><name sortKey="Fonseca, Alvaro" sort="Fonseca, Alvaro" uniqKey="Fonseca A" first="Álvaro" last="Fonseca">Álvaro Fonseca</name><name sortKey="Guerreiro, Marco A" sort="Guerreiro, Marco A" uniqKey="Guerreiro M" first="Marco A." last="Guerreiro">Marco A. Guerreiro</name><name sortKey="Heitman, Joseph" sort="Heitman, Joseph" uniqKey="Heitman J" first="Joseph" last="Heitman">Joseph Heitman</name><name sortKey="Rodrigues, Joana A" sort="Rodrigues, Joana A" uniqKey="Rodrigues J" first="Joana A." last="Rodrigues">Joana A. Rodrigues</name><name sortKey="Rusche, Laura N" sort="Rusche, Laura N" uniqKey="Rusche L" first="Laura N." last="Rusche">Laura N. Rusche</name><name sortKey="Springer, Deborah J" sort="Springer, Deborah J" uniqKey="Springer D" first="Deborah J." last="Springer">Deborah J. Springer</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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