Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE.
Identifieur interne : 000033 ( PubMed/Corpus ); précédent : 000032; suivant : 000034Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE.
Auteurs : Nele Weyens ; Safiyh Taghavi ; Tanja Barac ; Daniel Van Der Lelie ; Jana Boulet ; Tom Artois ; Robert Carleer ; Jaco VangronsveldSource :
- Environmental science and pollution research international [ 1614-7499 ] ; 2009.
English descriptors
- KwdEn :
- Bacteria (genetics), Bacteria (metabolism), DNA, Bacterial (genetics), Fraxinus (microbiology), Phylogeny, Plant Transpiration (physiology), Quercus (microbiology), RNA, Ribosomal, 16S (genetics), Soil (analysis), Soil Microbiology, Soil Pollutants (chemistry), Soil Pollutants (metabolism), Trichloroethylene (chemistry), Trichloroethylene (metabolism).
- MESH :
- chemical , analysis : Soil.
- chemical , chemistry : Soil Pollutants, Trichloroethylene.
- chemical , genetics : DNA, Bacterial, RNA, Ribosomal, 16S.
- genetics : Bacteria.
- metabolism : Bacteria, Soil Pollutants, Trichloroethylene.
- microbiology : Fraxinus, Quercus.
- physiology : Plant Transpiration.
- Phylogeny, Soil Microbiology.
Abstract
Along transects under a mixed woodland of English Oak (Quercus robur) and Common Ash (Fraxinus excelsior) growing on a trichloroethylene (TCE)-contaminated groundwater plume, sharp decreases in TCE concentrations were observed, while transects outside the planted area did not show this remarkable decrease. This suggested a possibly active role of the trees and their associated bacteria in the remediation process. Therefore, the cultivable bacterial communities associated with both tree species growing on this TCE-contaminated groundwater plume were investigated in order to assess the possibilities and practical aspects of using these common native tree species and their associated bacteria for phytoremediation. In this study, only the cultivable bacteria were characterized because the final aim was to isolate TCE-degrading, heavy metal resistant bacteria that might be used as traceable inocula to enhance bioremediation.
DOI: 10.1007/s11356-009-0154-0
PubMed: 19401827
Links to Exploration step
pubmed:19401827Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE.</title><author><name sortKey="Weyens, Nele" sort="Weyens, Nele" uniqKey="Weyens N" first="Nele" last="Weyens">Nele Weyens</name><affiliation><nlm:affiliation>Environmental Biology, Hasselt University, Agoralaan Building D, 3590, Diepenbeek, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Taghavi, Safiyh" sort="Taghavi, Safiyh" uniqKey="Taghavi S" first="Safiyh" last="Taghavi">Safiyh Taghavi</name></author><author><name sortKey="Barac, Tanja" sort="Barac, Tanja" uniqKey="Barac T" first="Tanja" last="Barac">Tanja Barac</name></author><author><name sortKey="Van Der Lelie, Daniel" sort="Van Der Lelie, Daniel" uniqKey="Van Der Lelie D" first="Daniel" last="Van Der Lelie">Daniel Van Der Lelie</name></author><author><name sortKey="Boulet, Jana" sort="Boulet, Jana" uniqKey="Boulet J" first="Jana" last="Boulet">Jana Boulet</name></author><author><name sortKey="Artois, Tom" sort="Artois, Tom" uniqKey="Artois T" first="Tom" last="Artois">Tom Artois</name></author><author><name sortKey="Carleer, Robert" sort="Carleer, Robert" uniqKey="Carleer R" first="Robert" last="Carleer">Robert Carleer</name></author><author><name sortKey="Vangronsveld, Jaco" sort="Vangronsveld, Jaco" uniqKey="Vangronsveld J" first="Jaco" last="Vangronsveld">Jaco Vangronsveld</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19401827</idno><idno type="pmid">19401827</idno><idno type="doi">10.1007/s11356-009-0154-0</idno><idno type="wicri:Area/PubMed/Corpus">000033</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000033</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE.</title><author><name sortKey="Weyens, Nele" sort="Weyens, Nele" uniqKey="Weyens N" first="Nele" last="Weyens">Nele Weyens</name><affiliation><nlm:affiliation>Environmental Biology, Hasselt University, Agoralaan Building D, 3590, Diepenbeek, Belgium.</nlm:affiliation></affiliation></author><author><name sortKey="Taghavi, Safiyh" sort="Taghavi, Safiyh" uniqKey="Taghavi S" first="Safiyh" last="Taghavi">Safiyh Taghavi</name></author><author><name sortKey="Barac, Tanja" sort="Barac, Tanja" uniqKey="Barac T" first="Tanja" last="Barac">Tanja Barac</name></author><author><name sortKey="Van Der Lelie, Daniel" sort="Van Der Lelie, Daniel" uniqKey="Van Der Lelie D" first="Daniel" last="Van Der Lelie">Daniel Van Der Lelie</name></author><author><name sortKey="Boulet, Jana" sort="Boulet, Jana" uniqKey="Boulet J" first="Jana" last="Boulet">Jana Boulet</name></author><author><name sortKey="Artois, Tom" sort="Artois, Tom" uniqKey="Artois T" first="Tom" last="Artois">Tom Artois</name></author><author><name sortKey="Carleer, Robert" sort="Carleer, Robert" uniqKey="Carleer R" first="Robert" last="Carleer">Robert Carleer</name></author><author><name sortKey="Vangronsveld, Jaco" sort="Vangronsveld, Jaco" uniqKey="Vangronsveld J" first="Jaco" last="Vangronsveld">Jaco Vangronsveld</name></author></analytic><series><title level="j">Environmental science and pollution research international</title><idno type="eISSN">1614-7499</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteria (genetics)</term><term>Bacteria (metabolism)</term><term>DNA, Bacterial (genetics)</term><term>Fraxinus (microbiology)</term><term>Phylogeny</term><term>Plant Transpiration (physiology)</term><term>Quercus (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Soil (analysis)</term><term>Soil Microbiology</term><term>Soil Pollutants (chemistry)</term><term>Soil Pollutants (metabolism)</term><term>Trichloroethylene (chemistry)</term><term>Trichloroethylene (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Soil</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Soil Pollutants</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteria</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacteria</term><term>Soil Pollutants</term><term>Trichloroethylene</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Fraxinus</term><term>Quercus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Transpiration</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Phylogeny</term><term>Soil Microbiology</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Along transects under a mixed woodland of English Oak (Quercus robur) and Common Ash (Fraxinus excelsior) growing on a trichloroethylene (TCE)-contaminated groundwater plume, sharp decreases in TCE concentrations were observed, while transects outside the planted area did not show this remarkable decrease. This suggested a possibly active role of the trees and their associated bacteria in the remediation process. Therefore, the cultivable bacterial communities associated with both tree species growing on this TCE-contaminated groundwater plume were investigated in order to assess the possibilities and practical aspects of using these common native tree species and their associated bacteria for phytoremediation. In this study, only the cultivable bacteria were characterized because the final aim was to isolate TCE-degrading, heavy metal resistant bacteria that might be used as traceable inocula to enhance bioremediation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19401827</PMID><DateCreated><Year>2009</Year><Month>11</Month><Day>02</Day></DateCreated><DateCompleted><Year>2009</Year><Month>11</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1614-7499</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>7</Issue><PubDate><Year>2009</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Environmental science and pollution research international</Title><ISOAbbreviation>Environ Sci Pollut Res Int</ISOAbbreviation></Journal><ArticleTitle>Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE.</ArticleTitle><Pagination><MedlinePgn>830-43</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s11356-009-0154-0</ELocationID><Abstract><AbstractText Label="BACKGROUND, AIM, AND SCOPE" NlmCategory="OBJECTIVE">Along transects under a mixed woodland of English Oak (Quercus robur) and Common Ash (Fraxinus excelsior) growing on a trichloroethylene (TCE)-contaminated groundwater plume, sharp decreases in TCE concentrations were observed, while transects outside the planted area did not show this remarkable decrease. This suggested a possibly active role of the trees and their associated bacteria in the remediation process. Therefore, the cultivable bacterial communities associated with both tree species growing on this TCE-contaminated groundwater plume were investigated in order to assess the possibilities and practical aspects of using these common native tree species and their associated bacteria for phytoremediation. In this study, only the cultivable bacteria were characterized because the final aim was to isolate TCE-degrading, heavy metal resistant bacteria that might be used as traceable inocula to enhance bioremediation.</AbstractText><AbstractText Label="MATERIALS AND METHODS" NlmCategory="METHODS">Cultivable bacteria isolated from bulk soil, rhizosphere, root, stem, and leaf were genotypically characterized by amplified rDNA restriction analysis (ARDRA) of their 16S rRNA gene and identified by 16S rRNA gene sequencing. Bacteria that displayed distinct ARDRA patterns were screened for heavy metal resistance, as well as TCE tolerance and degradation, as preparation for possible future in situ inoculation experiments. Furthermore, in situ evapotranspiration measurements were performed to investigate if the degradation capacity of the associated bacteria is enough to prevent TCE evapotranspiration to the air.</AbstractText><AbstractText Label="RESULTS AND DISCUSSION" NlmCategory="CONCLUSIONS">Between both tree species, the associated populations of cultivable bacteria clearly differed in composition. In English Oak, more species-specific, most likely obligate endophytes were found. The majority of the isolated bacteria showed increased tolerance to TCE, and TCE degradation capacity was observed in some of the strains. However, in situ evapotranspiration measurements revealed that a significant amount of TCE and its metabolites was evaporating through the leaves to the atmosphere.</AbstractText><AbstractText Label="CONCLUSIONS AND PERSPECTIVES" NlmCategory="CONCLUSIONS">The characterization of the isolates obtained in this study shows that the bacterial community associated with Oak and Ash on a TCE-contaminated site, was strongly enriched with TCE-tolerant strains. However, this was not sufficient to degrade all TCE before it reaches the leaves. A possible strategy to overcome this evapotranspiration to the atmosphere is to enrich the plant-associated TCE-degrading bacteria by in situ inoculation with endophytic strains capable of degrading TCE.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Weyens</LastName><ForeName>Nele</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Environmental Biology, Hasselt University, Agoralaan Building D, 3590, Diepenbeek, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taghavi</LastName><ForeName>Safiyh</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Barac</LastName><ForeName>Tanja</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>van der Lelie</LastName><ForeName>Daniel</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Boulet</LastName><ForeName>Jana</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Artois</LastName><ForeName>Tom</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Carleer</LastName><ForeName>Robert</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Vangronsveld</LastName><ForeName>Jaco</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>04</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Environ Sci Pollut Res Int</MedlineTA><NlmUniqueID>9441769</NlmUniqueID><ISSNLinking>0944-1344</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031661" MajorTopicYN="N">Fraxinus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018526" MajorTopicYN="N">Plant Transpiration</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029963" MajorTopicYN="N">Quercus</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012336" MajorTopicYN="N">RNA, Ribosomal, 16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012987" MajorTopicYN="N">Soil</DescriptorName><QualifierName UI="Q000032" 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